Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

Transaminase biocatalysis: optimization and application

(2017) Fei Guo et al.   GREEN CHEMISTRY

Biocatalytic transamination with near-stoichiometric inexpensive amine donors mediated by bifunctional mono- and di-amine transaminases

(2017) James L. Galman et al.   GREEN CHEMISTRY

A β-Alanine Catabolism Pathway Containing a Highly Promiscuous ω-Transaminase in the 12-Aminododecanate-Degrading Pseudomonas sp. Strain AAC

(2016) Matthew Wilding et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli

(2016) Nadine Ladkau et al.   METABOLIC ENGINEERING

On the Potential Origins of the High Stability of Reconstructed Ancestral Proteins

(2016) Devin L. Trudeau et al.   MOLECULAR BIOLOGY AND EVOLUTION

A quantum chemical study of the ω-transaminase reaction mechanism

(2015) Karim Engelmark Cassimjee et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Identification of novel transaminases from a 12-aminododecanoic acid-metabolizingPseudomonasstrain

(2015) Matthew Wilding et al.   Microbial Biotechnology

Strategic manipulation of an industrial biocatalyst - evolution of a cephalosporin C acylase

(2014) Gianluca Conti et al.   FEBS Journal

Mutational Studies on Resurrected Ancestral Proteins Reveal Conservation of Site-Specific Amino Acid Preferences throughout Evolutionary History

(2014) Valeria A. Risso et al.   MOLECULAR BIOLOGY AND EVOLUTION

Ancestral mutations as a tool for solubilizing proteins: The case of a hydrophobic phosphate-binding protein

(2014) Daniel Gonzalez et al.   FEBS Open Bio

Direct Terminal Alkylamino-FunctionalizationviaMultistep Biocatalysis in One Recombinant Whole-Cell Catalyst

(2013) Manfred Schrewe et al.   ADVANCED SYNTHESIS & CATALYSIS

Biocatalytic retrosynthesis

(2013) Nicholas J Turner et al.   Nature Chemical Biology

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

(2012) Sajid Noor et al.   PLoS One

Directed Evolution of Sulfotransferases and Paraoxonases by Ancestral Libraries

(2011) Uria Alcolombri et al.   JOURNAL OF MOLECULAR BIOLOGY

On the Origin and Evolution of Thermophily: Reconstruction of Functional Precambrian Enzymes from Ancestors of Bacillus

(2011) J. K. Hobbs et al.   MOLECULAR BIOLOGY AND EVOLUTION

Single-molecule paleoenzymology probes the chemistry of resurrected enzymes

(2011) Raul Perez-Jimenez et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Rational assignment of key motifs for function guides in silico enzyme identification

(2010) Matthias Höhne et al.   Nature Chemical Biology

Palaeotemperature trend for Precambrian life inferred from resurrected proteins

(2008) Eric A. Gaucher et al.   NATURE