Engineering of lactic acid bacteria for delivery of therapeutic proteins and peptides

Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications

(2018) Josef R. Bober et al.   Annual Review of Biomedical Engineering

Lipoteichoic acid mediates binding of a Lactobacillus S-layer protein

(2018) Eva Bönisch et al.   GLYCOBIOLOGY

p19-Targeting ILP Protein Blockers of IL-23/Th-17 Pro-Inflammatory Axis Displayed on Engineered Bacteria of Food Origin

(2018) Katja Škrlec et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Probiotic strains detect and suppress cholera in mice

(2018) Ning Mao et al.   Science Translational Medicine

Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickens

(2018) Linlin Liu et al.   VACCINE

Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis

(2018) Aleš Berlec et al.   Scientific Reports

Microbial Delivery Vehicles for Allergens and Allergen-Derived Peptides in Immunotherapy of Allergic Diseases

(2018) Abida Zahirović et al.   Frontiers in Microbiology

Versatile Cas9-Driven Subpopulation Selection Toolbox for Lactococcus lactis

(2018) Simon van der Els et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities

(2018) Katja Škrlec et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods

(2018) Ryan T. Leenay et al.   Biotechnology Journal

Genetically modified bacteria enlisted in fight against disease

(2018) Sara Reardon   NATURE

Lactic Acid Bacteria for Delivery of Endogenous or Engineered Therapeutic Molecules

(2018) Peter A. Bron et al.   Frontiers in Microbiology

Anti-cancer effect of lactic acid bacteria expressing antioxidant enzymes or IL-10 in a colorectal cancer mouse model

(2017) Silvina del Carmen et al.   INTERNATIONAL IMMUNOPHARMACOLOGY

Lactic acid bacteria - promising vaccine vectors: possibilities, limitations, doubts

(2017) K. Szatraj et al.   JOURNAL OF APPLIED MICROBIOLOGY

Cathelicidin-encodingLactococcus lactispromotes mucosal repair in murine experimental colitis

(2017) Clover C M Wong et al.   JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY

A review on Lactococcus lactis: from food to factory

(2017) Adelene Ai-Lian Song et al.   Microbial Cell Factories

Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000

(2017) Duolong Zhu et al.   Microbial Cell Factories

Living therapeutics: Scientists genetically modify bacteria to deliver drugs

(2017) Amy Maxmen   NATURE MEDICINE

Applications of Genetically Modified Immunobiotics with High Immunoregulatory Capacity for Treatment of Inflammatory Bowel Diseases

(2017) Suguru Shigemori et al.   Frontiers in Immunology

Evasin-displaying lactic acid bacteria bind different chemokines and neutralize CXCL8 production in Caco-2 cells

(2017) Katja Škrlec et al.   Microbial Biotechnology

Surface display on lactic acid bacteria without genetic modification: strategies and applications

(2016) Ruifeng Mao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis

(2016) Suguru Shigemori et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Lactic acid bacteria as mucosal delivery vehicles: a realistic therapeutic option

(2016) Miao Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recombinant Lactococcus lactis expressing porcine insulin-like growth factor I ameliorates DSS-induced colitis in mice

(2016) Shujie Liu et al.   BMC BIOTECHNOLOGY

A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei

(2016) W.W. Lu et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Reversal of Diabetes in NOD Mice by Clinical-Grade Proinsulin and IL-10–Secreting Lactococcus lactis in Combination With Low-Dose Anti-CD3 Depends on the Induction of Foxp3-Positive T Cells

(2016) Tatiana Takiishi et al.   DIABETES

Effect of recombinant Lactococcus lactis producing myelin peptides on neuroimmunological changes in rats with experimental allergic encephalomyelitis

(2016) Kaja Kasarełło et al.   FOLIA NEUROPATHOLOGICA

Immunization with r-Lactococcus lactisexpressing outer membrane protein A ofShigella dysenteriaetype-1: evaluation of oral and intranasal route of administration

(2016) B. Yagnik et al.   JOURNAL OF APPLIED MICROBIOLOGY

Critical Role of Antimicrobial Peptide Cathelicidin for ControllingHelicobacter pyloriSurvival and Infection

(2016) Lin Zhang et al.   JOURNAL OF IMMUNOLOGY

Construction of a new shuttle vector for DNA delivery into mammalian cells using non-invasive Lactococcus lactis

(2016) Bhrugu Yagnik et al.   MICROBES AND INFECTION

Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells

(2016) Bo Zhang et al.   Microbial Cell Factories

Display of recombinant proteins at the surface of lactic acid bacteria: strategies and applications

(2016) C. Michon et al.   Microbial Cell Factories

Engineering robust and tunable spatial structures with synthetic gene circuits

(2016) Wentao Kong et al.   NUCLEIC ACIDS RESEARCH

Heterologous Expression and Delivery of Biologically Active Exendin-4 by Lactobacillus paracasei L14

(2016) Zhu Zeng et al.   PLoS One

Characterization of the protective immune response to Yersinia pseudotuberculosis infection in mice vaccinated with an LcrV-secreting strain of Lactococcus lactis

(2016) Catherine Daniel et al.   VACCINE

Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis

(2016) Patrycja A. Kobierecka et al.   Frontiers in Microbiology

Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent

(2016) Ali Baradaran et al.   Journal of Cancer

Microbially produced glucagon-like peptide 1 improves glucose tolerance in mice

(2016) Tulika Arora et al.   Molecular Metabolism

In Vivo and In Vitro Detection of Luminescent and Fluorescent Lactobacillus reuteri and Application of Red Fluorescent mCherry for Assessing Plasmid Persistence

(2016) Shokoufeh Karimi et al.   PLoS One

Development of Recombinant Lactococcus lactis Displaying Albumin-Binding Domain Variants against Shiga Toxin 1 B Subunit

(2016) Petra Zadravec et al.   PLoS One

Oral Delivery of Pentameric Glucagon-Like Peptide-1 by Recombinant Lactobacillus in Diabetic Rats

(2016) Yin Lin et al.   PLoS One

Improvement of LysM-Mediated Surface Display of Designed Ankyrin Repeat Proteins (DARPins) in Recombinant and Nonrecombinant Strains of Lactococcus lactis and Lactobacillus Species

(2015) Petra Zadravec et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing

(2015) Evanna Huynh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protective immunity against influenza H5N1 virus challenge in chickens by oral administration of recombinant Lactococcus lactis expressing neuraminidase

(2015) Han Lei et al.   BMC Veterinary Research

Oral Immunization with a RecombinantLactococcus lactis–Expressing HIV-1 Antigen on Group AStreptococcusPilus Induces Strong Mucosal Immunity in the Gut

(2015) Venkateswarlu Chamcha et al.   JOURNAL OF IMMUNOLOGY

Programmable probiotics for detection of cancer in urine

(2015) Tal Danino et al.   Science Translational Medicine

Recombinant interleukin 6 with M cell-targeting moiety produced in Lactococcus lactis IL1403 as a potent mucosal adjuvant for peroral immunization

(2015) Hui-Shan Li et al.   VACCINE

Trimming of two major type 1 diabetes driving antigens, GAD65 and IA-2, allows for successful expression inLactococcus lactis

(2015) S. Robert et al.   Beneficial Microbes

Heterologous surface display on lactic acid bacteria: non-GMO alternative?

(2015) Petra Zadravec et al.   Bioengineered

Exploiting the peptidoglycan-binding motif, LysM, for medical and industrial applications

(2014) Ganesh Ram R. Visweswaran et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Oral Delivery of Glutamic Acid Decarboxylase (GAD)-65 and IL10 by Lactococcus lactis Reverses Diabetes in Recent-Onset NOD Mice

(2014) S. Robert et al.   DIABETES

Heme Oxygenase-1 Ameliorates Dextran Sulfate Sodium-induced Acute Murine Colitis by Regulating Th17/Treg Cell Balance

(2014) Liya Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Tunable protein degradation in bacteria

(2014) D Ewen Cameron et al.   NATURE BIOTECHNOLOGY

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Heterologous Protein Secretion in Lactobacilli with Modified pSIP Vectors

(2014) Ingrid Lea Karlskås et al.   PLoS One

Mucosal Vaccination with Recombinant Lactobacillus casei-Displayed CTA1-Conjugated Consensus Matrix Protein-2 (sM2) Induces Broad Protection against Divergent Influenza Subtypes in BALB/c Mice

(2014) Mohammed Y. E. Chowdhury et al.   PLoS One

Oral Administration of Recombinant Lactococcus lactis Expressing HSP65 and Tandemly Repeated P277 Reduces the Incidence of Type I Diabetes in Non-Obese Diabetic Mice

(2014) Yanjun Ma et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Oral vaccination against HPV E7 for treatment of cervical intraepithelial neoplasia grade 3 (CIN3) elicits E7-specific mucosal immunity in the cervix of CIN3 patients

(2014) Kei Kawana et al.   VACCINE

Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci

(2014) Juan Borrero et al.   ACS Synthetic Biology

Zirex: a Novel Zinc-Regulated Expression System for Lactococcus lactis

(2013) D. Mu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity

(2013) Aleš Berlec et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Lactococcus lactisAnchoring Avian Infectious Bronchitis Virus Multi-Epitope Peptide EpiC Induced Specific Immune Responses in Chickens

(2013) Hai-Peng CAO et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Phase 1b, multicenter, single blinded, placebo-controlled, sequential dose escalation study to assess the safety and tolerability of topically applied AG013 in subjects with locally advanced head and neck cancer receiving induction chemotherapy

(2013) Sewanti Atul Limaye et al.   CANCER

Oral Delivery of IL-27 Recombinant Bacteria Attenuates Immune Colitis in Mice

(2013) Miranda L. Hanson et al.   GASTROENTEROLOGY

Development of a Stress-Inducible Controlled Expression (SICE) system in Lactococcus lactis for the production and delivery of therapeutic molecules at mucosal surfaces

(2013) Bouasria Benbouziane et al.   JOURNAL OF BIOTECHNOLOGY

Engineering BmpA as a carrier for surface display of IgG-binding domain on Lactococcus lactis

(2013) P. Zadravec et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice

(2013) YIJIE LI et al.   Oncology Letters

Antimicrobial Peptides Targeting Gram-negative Pathogens, Produced and Delivered by Lactic Acid Bacteria

(2013) Katherine Volzing et al.   ACS Synthetic Biology

Relevance and application of sortase and sortase-dependent proteins in lactic acid bacteria

(2013) Emma K. Call et al.   Frontiers in Microbiology

Bioluminescence Imaging Study of Spatial and Temporal Persistence of Lactobacillus plantarum and Lactococcus lactis in Living Mice

(2012) Catherine Daniel et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering Signal Peptides for Enhanced Protein Secretion from Lactococcus lactis

(2012) Daphne T. W. Ng et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli

(2012) Tomás García-Cayuela et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

In vitro and in vivo characterization of DNA delivery using recombinant Lactococcus lactis expressing a mutated form of L. monocytogenes Internalin A

(2012) Marcela de Azevedo et al.   BMC MICROBIOLOGY

Construction and Immunological Evaluation of Dual Cell Surface Display of HIV-1 Gag and Salmonella enterica Serovar Typhimurium FliC in Lactobacillus acidophilus for Vaccine Delivery

(2012) Akinobu Kajikawa et al.   Clinical and Vaccine Immunology

Protective effects of cathelicidin-encoding Lactococcus lactis in murine ulcerative colitis

(2012) Clover Ching Man Wong et al.   JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY

High efficiency recombineering in lactic acid bacteria

(2012) Jan-Peter van Pijkeren et al.   NUCLEIC ACIDS RESEARCH

Food-Grade Bacteria Expressing Elafin Protect Against Inflammation and Restore Colon Homeostasis

(2012) J.-P. Motta et al.   Science Translational Medicine

pSEUDO, a Genetic Integration Standard for Lactococcus lactis

(2011) Joao P. C. Pinto et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Mucosal and Systemic Immune Responses Induced by Recombinant Lactobacillus spp. Expressing the Hemagglutinin of the Avian Influenza Virus H5N1

(2011) Zhisheng Wang et al.   Clinical and Vaccine Immunology

Lactococcus lactis-based vaccines: Current status and future perspectives

(2011) Mohammed Bahey-El-Din et al.   Human vaccines & immunotherapeutics

Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

(2011) Kayo Ohkouchi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Oral immunization with live Lactococcus lactis expressing rotavirus VP8* subunit induces specific immune response in mice

(2011) Belkis Marelli et al.   JOURNAL OF VIROLOGICAL METHODS

Targeted expression of anthrax protective antigen byLactobacillus gasserias an anthrax vaccine

(2010) Mansour Mohamadzadeh et al.   Future Microbiology

Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice

(2010) Laurie Watterlot et al.   INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

Immobilization of Lactococcus lactis to cellulosic material by cellulose-binding domain of Cellvibrio japonicus

(2010) K. Kylä-Nikkilä et al.   JOURNAL OF APPLIED MICROBIOLOGY

AG013, a mouth rinse formulation of Lactococcus lactis secreting human Trefoil Factor 1, provides a safe and efficacious therapeutic tool for treating oral mucositis

(2010) Silvia Caluwaerts et al.   ORAL ONCOLOGY

HSP65 serves as an immunogenic carrier for a diabetogenic peptide P277 inducing anti-inflammatory immune response in NOD mice by nasal administration

(2010) Jin Liang et al.   VACCINE

Translocation of a Thioether-Bridged Azurin Peptide Fragment via the Sec Pathway in Lactococcus lactis

(2009) A. Kuipers et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Lactococcus lactis Expressing either Staphylococcus aureus Fibronectin-Binding Protein A or Listeria monocytogenes Internalin A Can Efficiently Internalize and Deliver DNA in Human Epithelial Cells

(2009) S. Innocentin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Orally administered L. lactis secreting an anti-TNF Nanobody demonstrate efficacy in chronic colitis

(2009) K Vandenbroucke et al.   Mucosal Immunology

Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge

(2009) M. Mohamadzadeh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice

(2007) A. de Moreno de LeBlanc et al.   JOURNAL OF MEDICAL MICROBIOLOGY