期刊
JOURNAL OF BIOTECHNOLOGY
卷 168, 期 2, 页码 120-129出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.04.019
关键词
Lactic acid bacteria; Lactococcus lactis; Immunomodulation; Recombinant heterologous production; Stress-inducible system
In recent years, recombinant lactic acid bacteria (LAB) have been successfully used as safe mucosal delivery vectors. Herein, we report on the development of a Stress-Inducible Controlled Expression (SICE) system in L. lactis for the production and delivery of proteins of health interest (both therapeutic and vaccine related) at mucosal surfaces. This system is episomal in nature and is composed of a vector carrying an expression cassette under the transcriptional control of a stress-inducible promoter. The functionality of the SICE system was validated in vivo using two different routes of administration: oral and intranasal, and in two different murine models of human pathologies: (i) a model of therapy against inflammatory bowel diseases (IBD) and (ii) a model of vaccination against human papillomavirus type-16 (HPV-16). (C) 2013 Elsevier B.V. All rights reserved.
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