Article
Agriculture, Multidisciplinary
Bibek Byanju, Swastik Sen, Thomas Mansell, Buddhi P. P. Lamsal
Summary: Corn steep liquor (CSL) and thin stillage were evaluated as growth media for recombinant Lactococcus lactis to produce antifreeze proteins (AFPs). Light CSL and thin stillage were optimized with additives and trace elements to enhance the growth of wild-type and recombinant strains. The fermentation supernatants showed longer times to supercool and freeze, indicating potential use as antifreeze compounds in frozen food and non-food applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Shouli Yuan, Meng Dong, Hanlin Zhang, Hongde Xu, Qi Wang, Chunlong Yan, Rongcai Ye, Xiaoxiao Jiang, Huiqiao Zhou, Li Chen, Jun Cheng, Wen Xie, Wanzhu Jin
Summary: This study utilized a recombinant Lactococcus lactis expressing the TGF beta R2 extracellular domain to reduce liver fibrosis, showing promising results in both in vitro and in vivo experiments. The recombinant bacteria effectively inhibited collagen synthesis in hepatic stellate cells induced by TGF beta 1, and significantly reduced liver fibrosis in CCl4-treated mice. Additionally, treatment with the recombinant bacteria led to decreased levels of CD11b(+) Kupffer cells and fibrosis-related gene and protein expression. The subchronic toxicity test results showed no significant side effects of the engineered bacteria strain.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mey Fann Lee, Yu Wen Chu, Chu Hui Chiang, Rong San Jiang, Wei Wen Lin, Nancy M. M. Wang
Summary: Lactococcus lactis, a safe and crucial microorganism in the dairy industry, has been used to develop oral immunotherapy for allergies by introducing recombinant allergen-based vaccines. A triple-antigen vaccine formulation has been created and shown to be safe for repeated oral administration in rabbits.
FOOD BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nathalie N. S. E. Henriksen, Mads Frederik Hansen, Heiko T. Kiesewalter, Jakob Russel, Joseph Nesme, Kevin R. Foster, Birte Svensson, Gunnar Oregaard, Jakob Herschend, Mette Burmolle
Summary: This study shows that cultivation in a structured environment facilitates coexistence, evolution, and adaptation of bacterial communities. Co-cultivation in a biofilm model resulted in stable coexistence of two bacterial species and drove the evolution of high-yield variants. This study emphasizes the importance of structured environments for the cultivation and evolution of industrially important bacterial communities.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Alvaro Santibanez, Diego Paine, Mick Parra, Carlos Munoz, Natalia Valdes, Claudia Zapata, Rodrigo Vargas, Alex Gonzalez, Mario Tello
Summary: This study explored the therapeutic application of lactic acid bacteria producing interferon gamma in protecting rainbow trout and Atlantic salmon against pathogens. The results showed that oral administration of the recombinant probiotic stimulated a systemic immune response, doubling the survival rate in fish infected with F. psychrophilum, indicating biotechnological potential for application in aquaculture.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Keila Lopes Mendes, Deborah de Farias Lelis, Daniela Fernanda de Freitas, Luiz Henrique da Silveira, Alfredo Mauricio Batista de Paula, Andre Luiz Sena Guimaraes, Janaina Ribeiro Oliveira, Marileia Chaves Andrade, Sergio Avelino Mota Nobre, Sergio Henrique Sousa Santos
Summary: The study demonstrated that the combination of resveratrol and Lactococcus lactis can reduce body weight, improve lipid metabolism, and decrease the expression of inflammatory markers, showing potential in preventing the development of liver diseases.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Microbiology
Camila Prosperi de Castro, Bianca Mendes Souza, Pamela Mancha-Agresti, Vanessa Bastos Pereira, Meritxell Zurita-Turk, Tatiane Melo Preisser, Vanessa Pecini da Cunha, Janete Soares Coelho dos Santos, Sophie Yvette Leclercq, Vasco Azevedo, Anderson Miyoshi
Summary: The development of a new tuberculosis vaccine strategy utilizing a lactococcal DNA vaccine fusion of ESAT-6 and Ag85A has shown promising results in mouse immunization studies, inducing significant cellular and humoral immune responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Min Li, Weicheng Li, Dongyu Li, Juanjuan Tian, Luyao Xiao, Lai-Yu Kwok, Wei Li, Zhihong Sun
Summary: Exopolysaccharides (EPSs) from Lactococcus lactis subsp. lactis IMAU11823 were found to have strong reducing power and no cytotoxicity. The EPSs had different sugar compositions and molecular weights, with EPS-1 being a linear structure composed of glucose and mannose, and EPS-2 being a more complex, non-linear structure composed of mannose, glucose, and rhamnose. Furthermore, a biosynthesis model for EPS production in the IMAU11823 strain was proposed. This study broadens the understanding of the formation, structure, and function of complex EPSs produced by Lactococcus lactis subsp. lactis IMAU11823.
Article
Food Science & Technology
Taeok Kim, Shakti Chandra Mondal, Chae-Rim Jeong, So-Rim Kim, O-Hyun Ban, Young Hoon Jung, Jungwoo Yang, Soo-Jung Kim
Summary: The research aimed to assess the safety of Lactococcus lactis IDCC 2301 isolated from homemade cheese, using in vitro and in vivo assays to evaluate its antibiotic resistance, hemolytic activity, toxin production, infectivity, and metabolic activity. The results showed that the strain was susceptible to nine antibiotics suggested by EFSA, did not possess toxigenic genes or antibiotic resistance, did not exhibit hemolytic or beta-glucuronidase activity, and did not produce D-lactate or biogenic amines. Single-dose oral toxicity tests in rats showed no toxicity or mortality, indicating that Lactococcus lactis IDCC 2301 can be safely used as probiotics for human consumption.
FOOD SCIENCE & NUTRITION
(2022)
Article
Food Science & Technology
Xin Shen, Weicheng Li, Hongyu Cai, Shuai Guo, Min Li, Yangshuo Liu, Zhihong Sun
Summary: This study analyzed the fermentation capacity and metabolic profiles of 17 L. lactis subsp. lactis strains and found significant metabolic differences between strains with different fermentation rates. Peptides, esters, and tributyrin were identified as biomarkers to distinguish between fast and slow fermentation groups.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Oncology
Katarzyna Kaczmarek, Jerzy Wieckiewicz, Kazimierz Weglarczyk, Maciej Siedlar, Jarek Baran
Summary: This study investigated the use of genetically modified Lactococcus lactis bacteria for local delivery of human soluble TRAIL in CRC treatment. The results showed selective elimination of CRC cells and enhanced anti-tumor activity with oral administration of Metformin.
Article
Biotechnology & Applied Microbiology
Valerie Laroute, Nathalie Aubry, Marjorie Audonnet, Muriel Mercier-Bonin, Marie-Line Daveran-Mingot, Muriel Cocaign-Bousquet
Summary: This study combined genotypic and phenotypic approaches to investigate the diversity of GABA production in lactococci. Through comparative genomic analysis and phenotypic analysis, it was found that GABA production varied widely among different strains and species, and the regulation of GAD gene expression was identified as the major contributor to this diversity.
MICROBIAL CELL FACTORIES
(2023)
Review
Biochemistry & Molecular Biology
Annie Frelet-Barrand
Summary: Membrane proteins play crucial roles in cellular processes, but expressing sufficient amounts of them remains a challenge. Lactococcus lactis has emerged as an alternative expression system for membrane proteins from both prokaryotic and eukaryotic origins.
Article
Microbiology
Marina Kurbanova, Roman Voroshilin, Oksana Kozlova, Victor Atuchin
Summary: This study presents an in silico investigation into the influence of starter cultures on the bioactivity of peptides in cheese. The findings suggest that starter cultures, particularly Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris, have a significant impact on the formation of bioactive peptides.
Article
Endocrinology & Metabolism
Sofie Robert, Conny Gysemans, Tatiana Takiishi, Hannelie Korf, Isabella Spagnuolo, Guido Sebastiani, Karolien Van Huynegem, Lothar Steidler, Silvia Caluwaerts, Pieter Demetter, Clive H. Wasserfall, Mark A. Atkinson, Francesco Dotta, Pieter Rottiers, Tom L. Van Belle, Chantal Mathieu
Article
Medicine, Research & Experimental
Tatiana Takiishi, Hannelie Korf, Tom L. Van Belle, Sofie Robert, Fabio A. Grieco, Silvia Caluwaerts, Letizia Galleri, Isabella Spagnuolo, Lothar Steidler, Karolien Van Huynegem, Pieter Demetter, Clive Wasserfall, Mark A. Atkinson, Francesco Dotta, Pieter Rottiers, Conny Gysemans, Chantal Mathieu
JOURNAL OF CLINICAL INVESTIGATION
(2012)
Article
Immunology
Dana P. Cook, Joao Paulo Monteiro Carvalho Mori Cunha, Pieter-Jan Martens, Gabriele Sassi, Francesca Mancarella, Giuliana Ventriglia, Guido Sebastiani, An-Sofie Vanherwegen, Mark A. Atkinson, Karolien Van Huynegem, Lothar Steidler, Silvia Caluwaerts, Pieter Rottiers, Luc Teyton, Francesco Dotta, Conny Gysemans, Chantal Mathieu
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Endocrinology & Metabolism
Chantal Mathieu, Alice Wiedeman, Karen Cerosaletti, S. Alice Long, Elisavet Serti, Laura Cooney, Joan Vermeiren, Silvia Caluwaerts, Karolien Van Huynegem, Lothar Steidler, Sven Blomme, Pieter Rottiers, Gerald T. Nepom, Kevan C. Herold
Summary: The oral delivery of AG019 was found to be well tolerated and safe as a monotherapy and in combination with teplizumab in patients with type 1 diabetes. The treatment did not interfere with the safety profile of teplizumab and may have additional biological effects, including changes in preproinsulin-specific T cells. Preliminary data support further studies with AG019 alone or in combination with teplizumab or other systemic immunotherapies for type 1 diabetes.
Article
Endocrinology & Metabolism
Silvia Caluwaerts, Suzan Lambin, Rita van Bree, Herman Peeters, Ignace Vergote, Johan Verhaeghe
METABOLISM-CLINICAL AND EXPERIMENTAL
(2007)
Article
Oncology
A. Coosemans, S. Amini Nik, S. Caluwaerts, S. Lambin, G. Verbist, R. Van Bree, V. Schelfhout, E. de Jonge, I. Dalle, G. Jacomen, J. J. Cassiman, Ph. Moerman, I. Vergote, F. Amant
EUROPEAN JOURNAL OF CANCER
(2007)
Article
Endocrinology & Metabolism
S Caluwaerts, K Holemans, R van Bree, J Verhaeghe, FA Van Assche
METABOLISM-CLINICAL AND EXPERIMENTAL
(2006)
Article
Developmental Biology
S Caluwaerts, L Vercruysse, C Luyten, R Pijnenborg
Article
Obstetrics & Gynecology
J Verhaeghe, R van Bree, S Lambin, S Caluwaerts
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
(2005)
Article
Peripheral Vascular Disease
R Dechend, P Gratze, G Wallukat, E Shagdarsuren, R Plehm, JH Bräsen, A Fiebeler, W Schneider, S Caluwaerts, L Vercruysse, R Pijnenborg, FC Luft, DN Müller
Review
Obstetrics & Gynecology
K Holemans, S Caluwaerts, L Poston, FA Van Assche
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2004)
Article
Obstetrics & Gynecology
S Caluwaerts, K Holemans, R van Bree, J Verhaeghe, FA Van Assche
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
(2003)
Letter
Medicine, General & Internal
K Holemans, S Caluwaerts, FA Van Assche
Article
Immunology
S Caluwaerts, R Pijnenborg, C Luyten, JC Keith, FA Van Assche
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2002)
Article
Oncology
Vanita Noronha, Aditya Dhanawat, Vijay Maruti Patil, Nandini Menon, Ajay Kumar Singh, Pankaj Chaturvedi, Prathamesh Pai, Devendra Chaukar, Sarbani Ghosh Laskar, Kumar Prabhash
Summary: Neo-adjuvant chemotherapy has shown long term survival benefits in patients with locally advanced oral cavity squamous cell carcinomas.
Article
Oncology
Andrea Costantino, Claudio Sampieri, Gian Marco Pace, Bianca Maria Festa, Luca Cerri, Giorgio Gregory Giordano, Michael Dale, Giuseppe Spriano, Giorgio Peretti, Armando De Virgilio
Summary: This study aimed to predict the necessity of enteral nutrition at 28 days after surgery in patients with head and neck cancers. The results showed that the machine learning models had optimistic accuracy in predicting the need for enteral nutrition, but had moderate sensitivity.
Article
Oncology
Lirui Zhang, Qiaoshi Xu, Zhien Feng
Summary: This study presents a comprehensive clinical, genomic, and transcriptomic analysis of primary ghost cell odontogenic carcinoma (GCOC). The results provide the first comprehensive molecular atlas for primary GCOC, including previously identified CTNNB1 mutation and novel alterations in MAP3K, EP300, and the 22q11.21 region. The transcriptome analysis reveals significant involvement of cytokine-cytokine receptor interaction and the PI3K-Akt signaling pathway. More GCOC cases should be compared to validate these findings for accurate clinical guidance.
Article
Oncology
Emily Capone, Vittoria Perrotti, Ilaria Cela, Rossano Lattanzio, Lucrezia Togni, Corrado Rubini, Vito Carlo Alberto Caponio, Lorenzo Lo Muzio, Martina Colasante, Francesco Giansanti, Rodolfo Ippoliti, Stefano Iacobelli, Michael J. Wick, Nicole Spardy Burr, Gianluca Sala
Summary: This study explores the potential of galectin-3 binding protein (LGALS3BP) as a novel target for antibody-drug conjugate (ADC) therapy in adenoid cystic carcinoma (ACC). The results show that LGALS3BP is highly expressed in ACC tissues and treatment with anti-LGALS3BP ADC leads to durable tumor growth inhibition in animal models.
Article
Oncology
Andrea Costantino, Claudio Sampieri, Piero Giuseppe Meliante, Armando De Virgilio, Se-Heon Kim
Summary: This study compares the outcomes of transoral resections using the da Vinci SP and da Vinci Xi systems for oropharyngeal squamous cell carcinoma (OPSCC). The results indicate that the da Vinci SP system has advantages in terms of reduced docking and console times, as well as improved rates of wide negative resection margins. However, there were no significant differences in complication rates or postoperative functional outcomes between the two systems.