Article
Food Science & Technology
Jitka Laurent, Nore Struyf, An Bautil, Albina Bakeeva, Mikolaj Chmielarz, Marika Lyly, Beatriz Herrera-Malaver, Volkmar Passoth, Kevin J. Verstrepen, Christophe M. Courtin
Summary: A diet low in FODMAPs can help reduce symptoms in patients with irritable bowel syndrome, but avoiding cereal grain products may lead to nutritional deficiencies. Developing low-FODMAP, high-fiber cereal grain products using K. marxianus in bread production can effectively reduce fructan levels and maintain fructan and fructose levels below the threshold for low-FODMAP products.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sitong Chen, Zhaoxian Xu, Boning Ding, Yuwei Zhang, Shuangmei Liu, Chenggu Cai, Muzi Li, Bruce E. Dale, Mingjie Jin
Summary: This study utilized phylogeny-guided big data mining, rational modification, and ancestral sequence reconstruction strategies to discover 13 new active xylose isomerases (XIs) for Saccharomyces cerevisiae, demonstrating the importance of the amino-terminal fragment for maintaining basic XI activity. These XIs were used to construct four efficient xylose-utilizing S. cerevisiae strains, and the strain S. cerevisiae CRD5HS achieved ethanol titers as high as 85.95 and 94.76 g/liter from pretreated corn stover and corn cob, respectively, without detoxifying or washing pretreated biomass. The genetic regulation mechanisms obtained from adaptive laboratory evolution provide practical references for mining other scarce and valuable enzymes.
Article
Biotechnology & Applied Microbiology
Cristobal A. Onetto, Anthony R. Borneman, Simon A. Schmidt
Summary: Interactions between non-Saccharomyces yeast species and Saccharomyces cerevisiae can impact fermentation performance, with strain selection playing a key role in optimizing fermentation outcomes when using non-Saccharomyces species.
FERMENTATION-BASEL
(2021)
Review
Biology
Kobi Simpson-Lavy, Martin Kupiec
Summary: Glucose is the preferred carbon source for most cells. Yeast Snf1 is the main regulator of the response to low glucose availability. Three different mechanisms, including phosphorylation, SUMOylation, and protonation, independently control Snf1 activity, allowing a flexible response to carbon source availability.
Review
Biotechnology & Applied Microbiology
P. W. J. Labuschagne, B. Divol
Summary: Thiamine is crucial for yeast growth and the production of flavor compounds during alcoholic fermentation, with its concentration affecting fermentation kinetics and wine sensory properties. Its utilization and metabolism in Saccharomyces cerevisiae are influenced by various factors, making it important to optimize thiamine concentrations for industrial yeast applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Akihito Nakanishi, Kuan Zhang, Riri Matsumoto, Naotaka Yamamoto
Summary: In this study, glycerol-assimilating strains were isolated from S. cerevisiae BY4741, and the changes in biomass production, ethanol fermentation, and transcription levels related to glycolysis and the tricarboxylic acid cycle under aerobic and slightly anaerobic conditions were analyzed. The results showed differences in biomass production, ethanol yields, and transcription levels between wildtype and glycerol-assimilating strains under aerobic conditions. Differences in transcription levels downstream of glycolysis were also observed under slightly anaerobic conditions. The study suggests that redox imbalance in glycerol-acclimatized strains may hinder glycerol utilization.
Article
Biotechnology & Applied Microbiology
Esther Molina-Menor, Angela Vidal-Verdu, Carlos Gomis-Olcina, Juli Pereto, Manuel Porcar
Summary: In this study, a 3D printed polylactic acid matrix was used to enhance yeast growth and alter protein expression patterns in liquid media. The matrix also showed differences in sugar assimilation and ethanol production. The findings suggest that these matrixes may have potential applications in yeast fermentation-based biotechnology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Tara K. Bartolec, Joshua J. Hamey, Andrew Keller, Juan D. Chavez, James E. Bruce, Marc. R. Wilkins
Summary: This study investigates the function of histidine protein methyltransferase Hpm1p in yeast and confirms its association with diverse phenotypes, including sugar metabolism regulation, through techniques such as mass spectrometry analysis.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Biotechnology & Applied Microbiology
Zahabiya Malubhoy, Frederico Mendonca Bahia, Sophie Claire de Valk, Erik de Hulster, Toni Rendulic, Juan Paulo Ragas Ortiz, Joeline Xiberras, Mathias Klein, Robert Mans, Elke Nevoigt
Summary: The current study aimed to improve the flux into the reverse TCA (rTCA) pathway and achieve a higher carbon dioxide (CO2)-fixation and succinic acid (SA) yield. By changing the design of the expression cassettes for the rTCA pathway, overexpressing PYC2, and adding CaCO3 to the batch fermentations, an SA yield of 0.63 Cmol Cmol(-1) was achieved on glycerol, which is 47.1% of the theoretical maximum. The results suggest that the major part of dicarboxylic acids in the engineered strain was formed via the rTCA pathway, enabling a net fixation of CO2. The greatly increased capacity of the rTCA pathway and the overexpression of PYC2 further strengthened this capacity.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Fanny Bordet, Remy Romanet, Florian Bahut, Jordi Ballester, Camille Eicher, Cristina Pena, Vicente Ferreira, Regis Gougeon, Anne Julien-Ortiz, Chloe Roullier-Gall, Herve Alexandre
Summary: Yeast co-inoculations in winemaking can result in new aromatic expressions that are not present in the original pure cultures, due to yeast interactions. This study investigated the impact of three cocultures and their corresponding pure cultures on the chemical composition and sensory profile of Chardonnay wine. Esters, fatty acids, and phenol families were affected. The sensory profiles and metabolome of cocultures and pure cultures were found to be different, indicating the influence of interaction. High resolution mass spectrometry identified thousands of coculture biomarkers, with most of them being related to nitrogen metabolism.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Kirsty Black, Graeme Walker
Summary: The production of neutral distilled spirits, such as vodka and gin, is growing globally. This paper discusses the differences between the yeast strains used in neutral spirits and those used in whisky, rum, and brandy fermentation. It also describes the raw materials, yeasts, and fermentation conditions involved in neutral spirit production and explores challenges and opportunities in the technology, including advancements in improving yeast strains.
APPLIED SCIENCES-BASEL
(2023)
Article
Multidisciplinary Sciences
Kevy Pontes Eliodorio, Gabriel Caetano de Gois e Cunha, Felipe Senne de Oliveira Lino, Morten Otto Alexander Sommer, Andreas Karoly Gombert, Reinaldo Giudici, Thiago Olitta Basso
Summary: We developed a fully defined medium called 2SMol that mimics sugarcane molasses and allows for reproducibility and comparability in different laboratories. By validating the medium in actual molasses-based media, we demonstrated its flexibility and investigated the effect of nitrogen availability on ethanol yield during fermentation.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Applied
Hideaki Tokuyama, Ryo Aoyagi, Kazuto Fujita, Yuki Maekawa, Shohei Riya
Summary: Ethanol fermentation using a hybrid system containing free and immobilized yeast cells in macroporous hydrogel scaffold was studied, with immobilization achieved through NIPA and MTGA hydrogels. The hybrid system showed higher ethanol productivity than the control system, with macroporous hydrogel allowing stable growth of immobilized yeast cells. In steady-state fermentation, the hybrid system had significantly higher cell densities and ethanol productivities compared to the control system.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Article
Multidisciplinary Sciences
Alan Gasinski, Joanna Kawa-Rygielska, Agnieszka Kita, Alicja Kucharska
Summary: In recent years, beer brewers have been experimenting with using different substrates, other than the traditional ingredients, for beer production. In this study, purple-fleshed potatoes were utilized as a replacement for a portion of barley malt in the production of beer. The results showed that the beer samples exhibited enhanced antioxidant activity, higher concentration of anthocyanins and polyphenol compounds, as well as modified composition of volatiles and lower ethanol content. Additionally, the beer produced with the addition of 30% purple potatoes was deemed acceptable in terms of sensory qualities.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Arslan Ahmad, Summar A. Naqvi, Muhammad J. Jaskani, Muhammad Waseem, Ehsan Ali, Iqrar A. Khan, Muhammad Faisal Manzoor, Azhari Siddeeg, Rana Muhammad Aadil
Summary: Dates are rich in sugars and can be used for bioethanol production through anaerobic fermentation. Researchers have found that date palm waste in Pakistan, the largest agricultural waste, can be a cost-effective source for bioethanol production. By fermenting the waste with Saccharomyces cerevisiae, researchers have successfully produced 15% ethanol with the presence of glucose, fructose, and sucrose in the date waste, showing the potential for industrial ethanol production. Additionally, the proximate analysis of date fruit revealed various nutrients suitable for microbial growth for ethanol production.
FOOD SCIENCE & NUTRITION
(2021)
Meeting Abstract
Biochemical Research Methods
Aysem Batur, Haluk Hamamci, Ceylan Buyukkileci (Sahin), Ali Oguz Buyukkileci
Article
Environmental Sciences
Sirma Yegin, Ali Oguz Buyukkileci, Sayit Sargin, Yekta Goksungur
WASTE AND BIOMASS VALORIZATION
(2017)
Article
Agricultural Engineering
Kevser Sabanci, Ali Oguz Buyukkileci
BIORESOURCE TECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Ali Oguz Buyukkileci, Marcello Fernandez Lahore, Canan Tari
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2015)
Article
Chemistry, Applied
Can Ucuncu, Canan Tari, Hande Demir, Ali Oguz Buyukkileci, Banu Ozen
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
(2013)
Meeting Abstract
Biochemical Research Methods
Ceylan Buyukkileci, Ali Oguz Buyukkileci, Haluk Hamamci
Meeting Abstract
Biochemical Research Methods
Haluk Hamamci, Ali Oguz Buyukkileci, Nihal Destan Aytekin
Article
Chemistry, Applied
Nuket Zeybek, Robert A. Rastall, Ali Oguz Buyukkileci
CARBOHYDRATE POLYMERS
(2020)
Article
Agricultural Engineering
Ece Surek, Ali Oguz Buyukkileci, Sirma Yegin
Summary: A versatile process involving autohydrolysis and enzymatic hydrolysis was used to produce xylooligosaccharides (XOS) from hazelnut shells, with a maximum production of 22.5 g/L. The study demonstrated the potential of using A. pullulans xylanase for XOS production from agricultural waste materials.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Pharmacology & Pharmacy
Nuket Zeybek, Ali Oguz Buyukkileci, Sukru Gulec, Mehmet Polat, Hurriyet Polat
Summary: Long residence times, near-neutral pH values, and enzymatic release by resident microbiota provide unique opportunities for drug delivery in the colon. A colon-targeted drug delivery system composed of a xylan/chitosan composite shell on curcumin-loaded MSNs was developed. The xylan/chitosan shell showed excellent stability in the acidic upper GI tract and degradation triggered by resident microbiota in the colon. Additionally, the presence of xylan increased beneficial bacteria and improved short-chain fatty acid production for better colon health.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Energy & Fuels
Ali Oguz Buyukkileci, Nuran Temelli
Summary: Xylan, a renewable polysaccharide abundant in agricultural and forestry residues, can be hydrolyzed to produce xylooligosaccharides (XOS) and xylose. This study evaluated the use of organosolv pretreatment on corncob to retain xylan while removing lignin. The findings showed that treating corncob with 70% ethanol at 170 degrees C for 1 hour resulted in 50% lignin removal and 81% xylan recovery. Xylan digestibility and the production of xylose and XOS were achieved through enzymatic hydrolysis using commercial xylanases. The efficient utilization of xylan in lignocellulosic biorefineries can enhance sustainability.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Chemistry, Applied
Ece Surek, Ali Oguz Buyukkileci
CARBOHYDRATE POLYMERS
(2017)