Single-particle cryo-electron microscopy of macromolecular complexes

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

(2015) Thi Hoang Duong Nguyen et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

Structure of a yeast spliceosome at 3.6-angstrom resolution

(2015) Chuangye Yan et al.   SCIENCE

Structure of the Mammalian Ribosome-Sec61 Complex to 3.4 Å Resolution

(2014) Rebecca M. Voorhees et al.   CELL

Architecture of the Nitric-oxide Synthase Holoenzyme Reveals Large Conformational Changes and a Calmodulin-driven Release of the FMN Domain

(2014) Adam L. Yokom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Visualization of arrestin recruitment by a G-protein-coupled receptor

(2014) Arun K. Shukla et al.   NATURE

Structure of a modular polyketide synthase

(2014) Somnath Dutta et al.   NATURE

Structural rearrangements of a polyketide synthase module during its catalytic cycle

(2014) Jonathan R. Whicher et al.   NATURE

Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas

(2014) Christopher J Russo et al.   NATURE METHODS

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Yeast Mitochondrial Large Ribosomal Subunit

(2014) A. Amunts et al.   SCIENCE

Ultrastable gold substrates for electron cryomicroscopy

(2014) C. J. Russo et al.   SCIENCE

The Resolution Revolution

(2014) W. Kuhlbrandt   SCIENCE

Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy

(2014) G. McMullan et al.   ULTRAMICROSCOPY

Beam-induced motion correction for sub-megadalton cryo-EM particles

(2014) Sjors HW Scheres   eLife

Structure and Subunit Topology of the INO80 Chromatin Remodeler and Its Nucleosome Complex

(2013) Alessandro Tosi et al.   CELL

Protein disulfide engineering

(2013) Alan A. Dombkowski et al.   FEBS LETTERS

Quantitative characterization of electron detectors for transmission electron microscopy

(2013) Rachel S. Ruskin et al.   JOURNAL OF STRUCTURAL BIOLOGY

TRPV1 structures in distinct conformations reveal activation mechanisms

(2013) Erhu Cao et al.   NATURE

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Quantifying the local resolution of cryo-EM density maps

(2013) Alp Kucukelbir et al.   NATURE METHODS

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy

(2013) Shaoxia Chen et al.   ULTRAMICROSCOPY

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

(2013) Xiao-chen Bai et al.   eLife

Beam-induced motion of vitrified specimen on holey carbon film

(2012) Axel F. Brilot et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

Subunit order of eukaryotic TRiC/CCT chaperonin by cross-linking, mass spectrometry, and combinatorial homology modeling

(2012) N. Kalisman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DOLORS: Versatile Strategy for Internal Labeling and Domain Localization in Electron Microscopy

(2012) Pick-Wei Lau et al.   STRUCTURE

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

Fabs Enable Single Particle cryoEM Studies of Small Proteins

(2012) Shenping Wu et al.   STRUCTURE

Using Enhanced Sampling and Structural Restraints to Refine Atomic Structures into Low-Resolution Electron Microscopy Maps

(2012) Harish Vashisth et al.   STRUCTURE

Iterative Stable Alignment and Clustering of 2D Transmission Electron Microscope Images

(2012) Zhengfan Yang et al.   STRUCTURE

Tilt-Pair Analysis of Images from a Range of Different Specimens in Single-Particle Electron Cryomicroscopy

(2011) Richard Henderson et al.   JOURNAL OF MOLECULAR BIOLOGY

Client-Loading Conformation of the Hsp90 Molecular Chaperone Revealed in the Cryo-EM Structure of the Human Hsp90:Hop Complex

(2011) Daniel R. Southworth et al.   MOLECULAR CELL

Structural analysis of the core COMPASS family of histone H3K4 methylases from yeast to human

(2011) Y.-h. Takahashi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural flexibility of the G s  -helical domain in the  2-adrenoceptor Gs complex

(2011) G. H. Westfield et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates

(2011) B. S. Strunk et al.   SCIENCE

3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry

(2010) Xing Zhang et al.   CELL

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution

(2010) J.-P. Armache et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CryoEM structure of Hsp104 and its mechanistic implication for protein disaggregation

(2010) S. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Atomic Structure of Human Adenovirus by Cryo-EM Reveals Interactions Among Protein Networks

(2010) H. Liu et al.   SCIENCE

Zernike Phase Plate Cryoelectron Microscopy Facilitates Single Particle Analysis of Unstained Asymmetric Protein Complexes

(2010) Wei-Hau Chang et al.   STRUCTURE

Localization and orientation of the γ-Tubulin Small Complex components using protein tags as labels for single particle EM

(2009) Rebeca M. Choy et al.   JOURNAL OF STRUCTURAL BIOLOGY

Radiation damage effects at four specimen temperatures from 4 to 100K

(2009) Benjamin E. Bammes et al.   JOURNAL OF STRUCTURAL BIOLOGY

Molecular dynamics flexible fitting: A practical guide to combine cryo-electron microscopy and X-ray crystallography

(2009) Leonardo G. Trabuco et al.   METHODS

Detective quantum efficiency of electron area detectors in electron microscopy

(2009) G. McMullan et al.   ULTRAMICROSCOPY

Mechanism of Gate Opening in the 20S Proteasome by the Proteasomal ATPases

(2008) Julius Rabl et al.   MOLECULAR CELL

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

(2008) Xuekui Yu et al.   NATURE

The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae

(2008) Z. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De Novo Backbone Trace of GroEL from Single Particle Electron Cryomicroscopy

(2008) Steven J. Ludtke et al.   STRUCTURE

Flexible Fitting of Atomic Structures into Electron Microscopy Maps Using Molecular Dynamics

(2008) Leonardo G. Trabuco et al.   STRUCTURE