Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry
出版年份 2017 全文链接
标题
Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry
作者
关键词
Thawing, Centrifugation, Cell enumeration techniques, Viable cell counting, Centrifuges, Flow cytometry, White blood cells, Blood donors
出版物
PLoS One
Volume 12, Issue 11, Pages e0187440
出版商
Public Library of Science (PLoS)
发表日期
2017-11-02
DOI
10.1371/journal.pone.0187440
参考文献
相关参考文献
注意：仅列出部分参考文献，下载原文获取全部文献信息。- The effects of storage temperature on PBMC gene expression
- (2016) Jun Yang et al. BMC IMMUNOLOGY
- The effect of cellular isolation and cryopreservation on the expression of markers identifying subsets of regulatory T cells
- (2016) Weiying Zhang et al. JOURNAL OF IMMUNOLOGICAL METHODS
- The impact of cryopreservation on human peripheral blood leucocyte bioenergetics
- (2015) Kevin N. Keane et al. CLINICAL SCIENCE
- The effects of freeze/thawing on the function and phenotype of CD4+ lymphocyte subsets in normal individuals and patients with systemic lupus erythematosus
- (2015) Vanessa da Silva Ferreira et al. CRYOBIOLOGY
- Stable Phenotype Of B-Cell Subsets Following Cryopreservation and Thawing of Normal Human Lymphocytes Stored in a Tissue Biobank
- (2014) Simon Mylius Rasmussen et al. CYTOMETRY PART B-CLINICAL CYTOMETRY
- The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved Human Peripheral Blood Mononuclear Cells
- (2014) Marcella Sarzotti-Kelsoe et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Optimization of a whole blood phenotyping assay for enumeration of peripheral blood leukocyte populations in multicenter clinical trials
- (2014) Tiffany Hensley-McBain et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Effects of cryopreservation on effector cells for antibody dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity in 51Cr-release and CD107a assays
- (2014) Mariana M. Mata et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot
- (2014) Johannes Trück et al. Human Vaccines & Immunotherapeutics
- Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function
- (2013) Anja Germann et al. CRYOBIOLOGY
- Phenotypic and functional traits of peripheral blood mononuclear cells retained by controlled cryopreservation: implications for reliable sequential studies of dynamic interactions with endothelial cells
- (2013) Anike Lockmann et al. EXPERIMENTAL DERMATOLOGY
- Overnight Resting of PBMC Changes Functional Signatures of Antigen Specific T- Cell Responses: Impact for Immune Monitoring within Clinical Trials
- (2013) Sarah Kutscher et al. PLoS One
- Controlled-rate freezer cryopreservation of highly concentrated peripheral blood mononuclear cells results in higher cell yields and superior autologous T-cell stimulation for dendritic cell-based immunotherapy
- (2012) Timo Buhl et al. CANCER IMMUNOLOGY IMMUNOTHERAPY
- DIFFERENTIAL MODULATION OF PHENOTYPIC COMPOSITION OF HIV-INFECTED AND -UNINFECTED PBMCS DURING CRYOPRESERVATION
- (2012) Ashwini Shete et al. JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
- An optimized multi-parameter flow cytometry protocol for human T regulatory cell analysis on fresh and viably frozen cells, correlation with epigenetic analysis, and comparison of cord and adult blood
- (2012) L. Nettenstrom et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies
- (2012) Hari Ramachandran et al. Cells
- Cryopreservation modulates the detection of regulatory T cell markers
- (2011) Sebastian Sattui et al. CYTOMETRY PART B-CLINICAL CYTOMETRY
- Comparison of cryopreservation methods on T-cell responses to islet and control antigens from type 1 diabetic patients and controls
- (2011) B. Brooks-Worrell et al. DIABETES-METABOLISM RESEARCH AND REVIEWS
- Cryopreservation of monocytes or differentiated immature DCs leads to an altered cytokine response to TLR agonists and microbial stimulation
- (2011) Marjolein Meijerink et al. JOURNAL OF IMMUNOLOGICAL METHODS
- The Danish Blood Donor Study: a large, prospective cohort and biobank for medical research
- (2011) O. B. Pedersen et al. VOX SANGUINIS
- Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society
- (2010) R. Mallone et al. CLINICAL AND EXPERIMENTAL IMMUNOLOGY
- Bioequivalence comparison of a new freezing bag (CryoMACS®) with the Cryocyte® freezing bag for cryogenic storage of human hematopoietic progenitor cells
- (2010) Andreas Sputtek et al. CYTOTHERAPY
- Dimethyl sulfoxide (DMSO) exposure to human peripheral blood mononuclear cells (PBMCs) abolish T cell responses only in high concentrations and following coincubation for more than two hours
- (2010) Henrik Kloverpris et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation
- (2009) Eyad Elkord JOURNAL OF IMMUNOLOGICAL METHODS
- Concordant Proficiency in Measurement of T-Cell Immunity in Human Immunodeficiency Virus Vaccine Clinical Trials by Peripheral Blood Mononuclear Cell and Enzyme-Linked Immunospot Assays in Laboratories from Three Continents
- (2008) M. J. Boaz et al. Clinical and Vaccine Immunology
- Effects of cryopreservation on CD4+ CD25+ T cells of HIV-1 infected individuals
- (2008) Anna C. Seale et al. JOURNAL OF CLINICAL LABORATORY ANALYSIS
Find Funding. Review Successful Grants.
Explore over 25,000 new funding opportunities and over 6,000,000 successful grants.
ExploreCreate your own webinar
Interested in hosting your own webinar? Check the schedule and propose your idea to the Peeref Content Team.
Create Now