期刊
CRYOBIOLOGY
卷 71, 期 3, 页码 507-510出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2015.10.149
关键词
Cryopreservation; Systemic lupus erythematosus; T lymphocytes; Apoptosis
资金
- Sao Paulo State Research Foundation (FAPESP) [2009/12930-7]
- Brazilian government agency CNPq [305064/2011-8]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [09/12930-7] Funding Source: FAPESP
Several studies report on lymphocyte phenotypic and functional abnormalities in Systemic Lupus Erythematosus (SLE). Freezing and thawing may alter functional and phenotypic properties of cells. We assessed the effect of the freezing/thawing process (FIT) on Th1 (CD3(+)CD4(+)CCR4(-)CXCR3(+)CCR5(+)), Th2 (CD3(+)CD4(+)CCR5(-)CXCR3(-)CCR4(+)), Th17 (CD3(+)CD4(+)CCR6(+)CD161(+)), and Treg (CD3(+)CD4(+)CD25(high)CD127(-)) cell cultures in healthy controls and SLE patients. F/T was associated with decreased frequency of Th2 and Th17 cells in cultures from SLE patients but not from controls. FIT was also associated with increased frequency of apoptotic cells, as measured by annexin V labeling, in all T cell subtypes analyzed, as well as increased cell proliferation, as measured by Ki-67 labeling, in all cells except Th1 from SLE patients. Thus, F/T can have differentiated effects on T lymphocyte subtypes from SLE patients and controls, and can have significant effects on cell death and proliferation. These findings should be carefully considered when designing and interpreting studies on functional and phenotypic aspects of T lymphocytes in SLE. (C) 2015 Elsevier Inc. All rights reserved.
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