期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 347, 期 1-2, 页码 87-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2009.06.001
关键词
T regulatory cell; Cryopreservation
Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. (C) 2009 Elsevier B.V. All rights reserved.
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