Article
Biochemistry & Molecular Biology
Manas Pratim Chakraborty, Sudipta Bhattacharyya, Souryadip Roy, Indira Bhattacharya, Rahul Das, Arindam Mukherjee
Summary: In this study, a stable Hck inhibitor was developed by functionalizing the 4-arylidene position of the fluorescent curcumin scaffold, showing selective interaction with the inactive conformation of Hck. The lead compound exhibited no inhibitory effects on three other kinases and may mediate cytotoxicity through inhibition of the SFK signaling pathway.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Maria C. Z. Meneghetti, Lucy Naughton, Conor O'Shea, Dindet S. E. Koffi Teki, Vincent Chagnault, Helena B. Nader, Timothy R. Rudd, Edwin A. Yates, Jose Kovensky, Gavin J. Miller, Marcelo A. Lima
Summary: Heparan sulfate is a ubiquitous carbohydrate in animals that plays important roles in biological processes and has potential therapeutic targets. However, its chemical heterogeneity makes it difficult to establish direct structure-function correlations. Through the use of NMR and chemical synthesis, this study explores how different sulfation patterns affect the geometry of the polysaccharide backbone and investigates the conformational changes caused by substitution with more stable S-glycosidic forms.
Correction
Biochemistry & Molecular Biology
C. Kerr, H. Szmacinski, M. L. Fisher, B. Nance, J. R. Lakowicz, A. Akbar, J. W. Keillor, T. Lok Wong, R. Godoy-Ruiz, E. A. Toth, D. J. Weber, R. L. Eckert
Summary: The paper has been corrected.
Article
Chemistry, Multidisciplinary
Zeng-Shuai Yan, Xiao-Lei Li, Yu-Qiang Ma, Hong-Ming Ding
Summary: This study investigates the interaction between graphene nanosheets (GN) and the spike protein of SARS-CoV-2 through molecular simulations. The results show that in the closed state, GN can disrupt the binding of the spike protein to ACE2 and promote the transition from the down conformation to the up conformation in the Omicron variant. Furthermore, GN can insert near the fusion peptide and prevent the detachment of S1 from the spike protein.
Article
Chemistry, Multidisciplinary
Ming Dong, Xiu Liu, Zhi-Yuan Zhang, Chengmao Yu, Bochao Huo, Chunju Li
Summary: A large-cavity carbazole macrocycle (1) was synthesized through condensation of a long and rigid monomer and paraformaldehyde. Compound 1 exhibits highly selective binding towards large-sized tetra(n-propyl) ammonium cation 3(+). The complexation of 3(+) by 1 is dependent on the counter anion, with Cl- showing the highest association constant of 3010 +/- 230 M-1.
CHEMICAL COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yanxiang Meng, Katherine A. Davies, Cheree Fitzgibbon, Samuel N. Young, Sarah E. Garnish, Christopher R. Horne, Cindy Luo, Jean-Marc Garnier, Lung-Yu Liang, Angus D. Cowan, Andre L. Samson, Guillaume Lessene, Jarrod J. Sandow, Peter E. Czabotar, James M. Murphy
Summary: MLKL pseudokinase is activated by the upstream kinase RIPK3 in the necroptotic pathway, but the structural basis of its activation remains unclear. This study reveals structural differences between human and murine RIPK3, shedding light on the mechanistic implications.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Moritz Bauer, Enrique Vidal, Eduard Zorita, Nil Uresin, Stefan F. Pinter, Guillaume J. Filion, Bernhard Payer
Summary: The study reveals the presence of A/B-like compartments on the inactive X chromosome, guiding TAD formation independently of transcription during X-reactivation. The formation of TADs and transcriptional reactivation are shown to be causally independent during X-reactivation, with Xist identified as a common factor.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yan Yan, Somnath Mukherjee, Kaleeckal G. Harikumar, Timothy S. Strutzenberg, X. Edward Zhou, Kelly Suino-Powell, Ting-Hai Xu, Ryan D. Sheldon, Jared Lamp, Joseph S. Brunzelle, Katarzyna Radziwon, Abigail Ellis, Scott J. Novick, Irving E. Vega, Russell G. Jones, Laurence J. Miller, H. Eric Xu, Patrick R. Griffin, Anthony A. Kossiakoff, Karsten Melcher
Summary: AMPK regulates cellular metabolism by modulating AL phosphorylation and accessibility, with ATP inhibiting its activity when levels are low. Researchers have developed antibodies and analyzed structures to reveal the mechanism by which adenine nucleotides and pharmacological agonists regulate AMPK activity.
Article
Biochemistry & Molecular Biology
Elizabeth R. Ballou, Atlanta G. Cook, Edward W. J. Wallace
Summary: The RNase II family of 3'-5' exoribonucleases is found across all domains of life, with eukaryotic members Dis3 and Dis3L2 playing essential roles in RNA degradation. Some yeasts contain both active Dis3 and inactive pseudonucleases, which function as RNA-binding proteins affecting cell growth, cytokinesis, and fungal pathogenicity. The evolutionary origins of these pseudonucleases remain unknown, with their non-nuclease functions requiring specific structural domains across diverse fungal lineages.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Editorial Material
Oncology
Phei Er Saw, Jianing Chen, Erwei Song
Summary: Neutrophil extracellular traps (NETs) are involved in both infection control and tumor metastasis. The study by Mousset et al. published in Cancer Cell reveals that chemotherapy-induced inflammation promotes NETosis in malignant tumors, leading to chemoresistance. This finding highlights the potential of targeting inflammatory NETs for cancer treatment.
Article
Biochemistry & Molecular Biology
Kwabena Owusu Dankwah, Jonathon E. Mohl, Khodeza Begum, Ming-Ying Leung
Summary: This study computationally predicted the binding of a single ligand to GPCRs from different families and uncovered similar binding pockets that contribute to ligand interactions. These findings can be applied to improve protein function inference, drug repurposing, drug toxicity prediction, and the acceleration of new drug development.
Article
Chemistry, Inorganic & Nuclear
Karina Abramov-Harpaz, Yifat Miller
Summary: The switching mechanism between the open-state conformation and the newly closed-state conformation of IDE is stabilized by electrostatic interactions between domain D1 and domain D3. The loss of a Zn2+ ion at the catalytic zinc-binding site in the open-state conformation of IDE prevents the transition to the closed-state conformation.
INORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Biochemistry & Molecular Biology
Kenji Okamoto, Yasushi Sako
Summary: This study used laser excitation technique to measure cytosolic RAF protein in live cells and analyzed its structural states through FRET analysis. The results showed that a 14-3-3 dimer bound to two sites on a single CRAF molecule and induced the formation of the autoinhibitory closed conformation. WT-CRAF mainly adopted two closed conformations, which showed different responsiveness to EGF stimulation.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Xianrui Gao, Hongjun Fan
Summary: This study investigates the modulating effect of the non-redox-inactive component Ca2+ on the redox potential of the photosynthetic oxygen-evolving center. The results show that the non-redox-inactive metal has a weak modulating effect on the potential, while the net charge of the complex has a significant impact. The potential can be regulated through protonation, deprotonation, or ligand modification.
INORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
David S. White, Sandipan Chowdhury, Vinay Idikuda, Ruohan Zhang, Scott T. Retterer, Randall H. Goldsmith, Baron Chanda
Summary: Research has shown both positive and negative cooperativity in cAMP binding, with limited parameter resolution in bulk measurements. By using nanophotonic zero-mode waveguides, individual ligand binding dynamics to multimeric ion channels have been directly resolved, revealing different kinetics in cAMP binding and isomerization. This approach can provide insights into binding allostery at single-molecule resolution in other intact membrane proteins and receptors.
Article
Medicine, General & Internal
Irene Rodriguez-Gomez, Stuart R. Gray, Frederick K. Ho, Fanny Petermann-Rocha, Paul Welsh, John Cleland, Stamatina Iliodromiti, Ignacio Ara, Jill Pell, Naveed Sattar, Lyn D. Ferguson, Carlos Celis-Morales
Summary: Male osteoporosis patients have a higher risk of developing cardiovascular disease, respiratory disease, and COPD, with a higher mortality risk. Female osteoporosis patients are more likely to develop cardiovascular disease, respiratory disease, and have a higher risk of mortality from respiratory disease and breast cancer.
MAYO CLINIC PROCEEDINGS
(2022)
Correction
Multidisciplinary Sciences
Christopher D. Go, James D. R. Knight, Archita Rajasekharan, Bhavisha Rathod, Geoffrey G. Hesketh, Kento T. Abe, Ji-Young Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y. Zhu, Evelyn Popiel, Jean-Philippe Lambert, Etienne Coyaud, Sally W. T. Cheung, Dushyandi Rajendran, Cassandra J. Wong, Hana Antonicka, Laurence Pelletier, Alexander F. Palazzo, Eric A. Shoubridge, Brian Raught, Anne-Claude Gingras
Review
Geriatrics & Gerontology
Fanny Petermann-Rocha, Viktoria Balntzi, Stuart R. Gray, Jose Lara, Frederick K. Ho, Jill P. Pell, Carlos Celis-Morales
Summary: The diagnosis and prevalence of sarcopenia vary considerably, and future studies should follow current guidelines to facilitate comparison of results across different studies and populations globally.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Endocrinology & Metabolism
Jirapitcha Boonpor, Fanny Petermann-Rocha, Solange Parra-Soto, Jill P. Pell, Stuart R. Gray, Carlos Celis-Morales, Frederick K. Ho
Summary: This study investigated the associations between different types of diet and incident type 2 diabetes, and found that fish eaters and fish and poultry eaters had a lower risk of developing type 2 diabetes compared to meat eaters, partially due to lower risk of obesity.
DIABETES OBESITY & METABOLISM
(2022)
Article
Sport Sciences
Jirapitcha Boonpor, Solange Parra-Soto, Fanny Petermann-Rocha, Frederick K. Ho, Carlos Celis-Morales, Stuart R. Gray
Summary: The study investigates the combined association of walking pace and grip strength with incident type 2 diabetes. The findings suggest that slower pace and weaker grip strength are associated with a higher risk of developing type 2 diabetes, independent of other factors.
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
(2022)
Article
Medicine, Research & Experimental
A. Morrow, Stuart R. Gray, H. K. Bayes, R. Sykes, E. McGarry, D. Anderson, D. Boiskin, C. Burke, J. G. F. Cleland, C. Goodyear, T. Ibbotson, C. C. Lang, McConnachie, F. Mair, K. Mangion, M. Patel, N. Sattar, D. Taggart, R. Taylor, S. Dawkes, C. Berry
Summary: This clinical trial aims to investigate the effect of resistance exercise intervention on exercise capacity and health status in individuals who have recovered from COVID-19. The study will include 220 adults diagnosed with COVID-19 and will compare the outcomes of usual care versus usual care plus resistance exercise intervention over a 12-week period. The primary outcome measure is the incremental shuttle walks test (ISWT) at three months post-randomisation.
Article
Multidisciplinary Sciences
Paula P. Coelho, Geoffrey G. Hesketh, Annika Pedersen, Elena Kuzmin, Anne-Marie N. Fortier, Emily S. Bell, Colin D. H. Ratcliffe, Anne-Claude Gingras, Morag Park
Summary: This study uncovers an LC3C-Endocytic-Associated-Pathway (LEAP) that selectively recruits plasma membrane cargo to autophagosomes, providing new understanding of the selective coupling of plasma membrane signaling with autophagic degradation.
NATURE COMMUNICATIONS
(2022)
Article
Geriatrics & Gerontology
Veronica Cabanas-Sanchez, Irene Esteban-Cornejo, Solange Parra-Soto, Fanny Petermann-Rocha, Stuart R. Gray, Fernando Rodriguez-Artalejo, Frederick K. Ho, Jill P. Pell, David Martinez-Gomez, Carlos Celis-Morales
Summary: Handgrip strength is inversely associated with incident depression and anxiety. It could be used as a simple, non-invasive, and inexpensive measure to stratify patients and identify those at elevated risk of mental health problems. Future research should investigate if resistance training can prevent the occurrence of mental health conditions.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Biology
Yong Zi Tan, Yazan M. Abbas, Jing Ze Wu, Di Wu, Kristine A. Keon, Geoffrey G. Hesketh, Stephanie A. Bueler, Anne-Claude Gingras, Carol Robinson, Sergio Grinstein, John L. Rubinstein
Summary: CryoEM analysis revealed an interaction between mEAK-7 and V-ATPase, which is disrupted by ATP-induced rotation. Exogenous mEAK-7 does not inhibit V-ATPase activity unlike its yeast homolog.
LIFE SCIENCE ALLIANCE
(2022)
Article
Medicine, General & Internal
Jirapitcha Boonpor, Frederick K. Ho, Stuart R. Gray, Carlos A. Celis-Morales
Summary: The study found that self-reported average and slow walking pace were associated with a higher risk of developing type 2 diabetes. This association was consistent across different physical activity levels and walking time.
MAYO CLINIC PROCEEDINGS
(2022)
Article
Rheumatology
Huey Yi Chong, Paul McNamee, Eva-Maria Bachmair, Kathryn Martin, Lorna Aucott, Neeraj Dhaun, Emma Dures, Richard Emsley, Stuart. R. Gray, Elizabeth Kidd, Vinod Kumar, Karina Lovell, Graeme MacLennan, John Norrie, Lorna Paul, Jonathan Packham, Stuart. H. Ralston, Stefan Siebert, Alison Wearden, Gary Macfarlane, Neil Basu
Summary: This study estimated the cost-effectiveness of combining cognitive behavioural approach (CBA) or personalized exercise programme (PEP) with usual care (UC) for patients with inflammatory rheumatic diseases and chronic, moderate to severe fatigue. The results showed that PEP was more expensive but significantly more effective than UC, while CBA was more expensive but not significantly more effective. Therefore, combining PEP with UC is likely to be a cost-effective use of healthcare resources.
Article
Sport Sciences
Maryam Soltanisarvestani, Nathan Lynskey, Stuart Gray, Jason M. R. Gill, Jill P. Pell, Naveed Sattar, Paul Welsh, Frederick K. Ho, Carlos Celis-Morales, Fanny Peterman-Rocha
Summary: This study investigated the associations between walking pace, grip strength, and mortality in stroke survivors. The results showed that low grip strength and slow walking pace were associated with a higher risk of stroke and all-cause mortality. Improving physical capability among stroke survivors may potentially prolong survival.
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
(2023)
Article
Endocrinology & Metabolism
Jirapitcha Boonpor, Jill P. Pell, Frederick K. Ho, Carlos Celis-Morales, Stuart R. Gray
Summary: Sarcopenia is associated with an increased risk of cardiovascular disease in people with type 2 diabetes, leading to earlier onset of the disease. Screening and prevention of sarcopenia in patients with type 2 diabetes may be useful in preventing complications of cardiovascular disease.
DIABETES OBESITY & METABOLISM
(2023)
Review
Nutrition & Dietetics
Maha Timraz, Ahmad Binmahfoz, Terry J. Quinn, Emilie Combet, Stuart R. Gray
Summary: The aim of this study was to examine the effects of LCn-3 PUFA supplementation on muscle strength in older individuals. A systematic literature review was conducted, and five relevant studies were included. The results showed that LCn-3 PUFA supplementation did not significantly improve grip strength compared to the control group.
Article
Rheumatology
Sarah E. Bennett, Celia Almeida, Eva-Maria Bachmair, Stuart R. Gray, Karina Lovell, Lorna Paul, Alison Wearden, Gary J. Macfarlane, Neil Basu, Emma Dures
Summary: This study aimed to understand the perspectives of rheumatology health professionals in delivering interventions in the LIFT trial. Therapists improved their clinical skills and satisfaction through training, collaboration with patients, and clinical supervision. Insights from this trial can enhance clinical practice and service provision.
RHEUMATOLOGY ADVANCES IN PRACTICE
(2022)
