Article
Chemistry, Applied
Artem O. Melekhin, Denis P. Bulkatov, Michael Yu. Parfenov, Vladimir V. Apyari, Veronika V. Tolmacheva
Summary: A rapid and simple LC-MS/MS method was proposed for simultaneous determination of polar and non-polar veterinary drugs in milk. The method utilized a robust dual column system and successfully separated 14 veterinary drugs with logP values ranging from -8.30 to 8.74. Validation of the method was performed by spiking blank milk samples with different concentrations of the analytes, and good sensitivity and reliability were observed with LOQs ranging from 1 to 50 μg kg-1 and relative standard deviations <20% for most substances.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Eunjung Kim, Sihyun Park, Hyunjin Park, Jangduck Choi, Hae Jung Yoon, Jeong-Han Kim
Summary: The study aimed to develop a comprehensive and simple method for the simultaneous determination of anthelmintic and antiprotozoal drug residues in fish. The QuEChERS method was used for sample preparation, followed by LC-MS/MS analysis, resulting in high accuracy and recovery rates.
Article
Chemistry, Analytical
Federica Castellani, Matteo Ricci, Maria Novella Colagrande, Giampiero Scortichini, Giorgio Saluti
Summary: A highly selective multi-residue method based on LC-Q-Orbitrap was developed and validated for the determination of 12 regulated coccidiostats in eggs and muscle. The method included ionophore antibiotics and synthetic coccidiostats. Sample preparation involved extraction with acetonitrile, clean-up with Oasis(R) PRiME HLB SPE, and defatting with n-hexane. Validation was performed according to EU regulation 2021/808, starting from 1 μg kg(-1). The method was verified using a certified reference material (CRM) and applied to the Italian National Residue Control Plan (NRCP).
Article
Endocrinology & Metabolism
Heleen Jansen, Antonius E. van Herwaarden, Henk J. Huijgen, Rebecca C. Painter, Jacquelien J. Hillebrand, Anita Boelen, Annemieke C. Heijboer
Summary: This study evaluated the accuracy of several immunoassay methods for measuring fT4 in the serum of pregnant women. The results showed that the immunoassays overestimated fT4 levels in pregnant women, potentially leading to an overestimation of thyroid function.
EUROPEAN THYROID JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Valentin Braun, Hermann Stuppner, Lorenz Risch, Christoph Seger
Summary: Steroid analysis in clinical laboratories is currently dominated by immunoassays, but liquid chromatography coupled to mass spectrometry (LC-MS/MS) has been proven to be a more capable tool with better sensitivity, specificity, and the ability to analyze multiple steroids and metabolites simultaneously, providing more reliable and comprehensive diagnostic information for endocrinologists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Anna Roszkowska, Natalia Treder, Alina Plenis, Natalia Miekus, Ilona Oledzka, Piotr Kowalski, Tomasz Baczek
Summary: This study compared and optimized two microextraction strategies for the extraction of four immunosuppressants in biofluids. The results showed that one strategy exhibited better performance for the extraction of a novel immunosuppressive drug.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Agriculture, Multidisciplinary
Myriam Bustamante-Rangel, Maria Milagros Delgado-Zamarreno, Encarnacion Rodriguez-Gonzalo
Summary: A simple and sensitive method has been developed for the analysis of anthelmintic drugs in milk, involving extraction using QuEChERS method and separation and determination by LC-MS/MS. This method provides high sensitivity for the determination of anthelmintics in milk samples and can be successfully used for routine analysis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Environmental Sciences
Marina Bellot, Carlos Barata, Cristian Gomez-Canela
Summary: The study evaluated the stability of 17 neuroactive compounds in water and found that most compounds did not degrade, except for apomorphine and 6-hydroxydopamine which degraded completely within 24 hours. Most compounds altered motile responses to light in the crustacean Daphnia magna.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Medical Laboratory Technology
Mikael Lindstrom, Mink Schinkelshoek, Pentti J. Tienari, Jyrki P. Kukkonen, Risto Renkonen, Rolf Fronczek, Gert Jan Lammers, Outi Itkonen
Summary: A liquid chromatography mass spectrometry assay (LC-MS/MS) was developed for the quantification of CSF orexin-A and B, revealing lower concentrations of orexin-A in narcolepsy type 1 patients compared to controls. Commercial radioimmunoassay (RIA) yielded higher concentrations than LC-MS/MS.
CLINICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Nadia Arbouche, Jean-Sebastin Raul, Pascal Kintz
Summary: Oral antidiabetics are drugs used to control blood sugar in diabetic subjects. The greatest risk of using these drugs is hypoglycemia, especially in individuals with no history of exposure to these medications. Hair testing for oral antidiabetics can help differentiate the cause of hypoglycemia and has a large detection window with long-term stability. A liquid-chromatography coupled to tandem mass spectrometry method has been developed and successfully applied to analyze 13 oral antidiabetics in hair samples from diabetic patients.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sunyeong Jang, Hyungju Seo, Hojin Kim, Hyoyoung Kim, Jongsung Ahn, Hyunjeong Cho, Sunghie Hong, Seunghwa Lee, Taewoong Na
Summary: A method for the rapid and quantitative analysis of 30 veterinary drugs in feed samples was developed and validated. The method showed good linearity and recovery rates, and can be used for screening residual multiclass veterinary drugs in feed samples.
Article
Chemistry, Analytical
Piotr Adamowicz, Joanna Bigosinska, Dominika Gil, Marta Suchan, Bogdan Tokarczyk
Summary: This study demonstrates the usefulness of fingerprints as an alternative material in forensic toxicology. Drugs can be detected in fingerprints even after single doses, with parent compounds dominating over metabolites in fingerprints. The detection window depends on the type of drug and duration of the treatment.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Review
Biochemistry & Molecular Biology
Cristian D'Ovidio, Marcello Locatelli, Miryam Perrucci, Luigi Ciriolo, Kenneth G. Furton, Isil Gazioglu, Abuzar Kabir, Giuseppe Maria Merone, Ugo de Grazia, Imran Ali, Antonio Maria Catena, Michele Treglia, Luigi T. Marsella, Fabio Savini
Summary: LC-MS/MS plays a crucial role in screening and tracking pharmacotoxicological and forensic cases due to its advanced features. This review paper focuses on its applications in pharmacotoxicology and highlights its importance in the development of pharmacological and forensic research. It discusses therapeutic drug monitoring and clinical approaches as well as the determination of illicit drugs.
Article
Environmental Sciences
Weimin Wang, Changsheng Guo, Like Chen, Ziwen Qiu, Xingxing Yin, Jian Xu
Summary: An enantioselective method was developed to quantify amphetamine-type chiral illicit drugs in wastewater and surface water samples from a wastewater treatment plant and its effluent-receiving river in Beijing, China. The method showed excellent performance in monitoring the variations of enantiomers in the wastewater treatment process.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Andrea Acosta-Dacal, Cristian Rial-Berriel, Ricardo Diaz-Diaz, Maria del Mar Bernal-Suarez, Manuel Zumbado, Luis Alberto Henriquez-Hernandez, Octavio P. Luzardo
Summary: A validated QuEChERS extraction method was developed for the analysis of ARs and PhACs residues in soil samples, proving to be effective for both agricultural monitoring and forensic investigations. The method was successfully validated in clay loam soil samples, with LOQ and LOD values within acceptable ranges.
Article
Biochemical Research Methods
Melissa Di Rocco, Mary Moloney, Deirdre Haren, Montserrat Gutierrez, Sean Earley, Bjorn Berendsen, Ambrose Furey, Martin Danaher
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Food Science & Technology
Antonio Lourenco, Maria Fraga-Corral, Lorenzo De Colli, Mary Moloney, Martin Danaher, Kieran Jordan
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Biochemical Research Methods
Lorenzo De Colli, Christopher Elliott, John Finnan, Jim Grant, Elke K. Arendt, Susan P. McCormick, Martin Danaher
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2020)
Article
Biochemical Research Methods
Tobias Lange, Andreas Thomas, Christian Goergens, Martin Bidlingmaier, Katharina Schilbach, Eric Fichant, Philippe Delahaut, Mario Thevis
Summary: Analytical methods were developed to detect the potential misuse of ghrelin mimetics in sports, identifying reliable target analytes through in vitro/in vivo experiments and high resolution mass spectrometry. The study also found numerous metabolites and implemented intact drugs into doping testing procedures for human urine and blood matrices, with the potential to enhance sensitivity, specificity, and detection window in sports drug testing programs for human athletes.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Environmental Sciences
Chris Fennell, Bruce Misstear, David O'Connell, Donata Dubber, Patrice Behan, Martin Danaher, Mary Moloney, Laurence Gill
Summary: Private wells in rural areas of Ireland are prone to microbial contamination, possibly from agriculture and domestic wastewater treatment systems. Traditional faecal indicator bacteria are not source-specific, but chemical contamination fingerprinting techniques can help identify the contamination sources more accurately.
ENVIRONMENTAL POLLUTION
(2021)
Review
Environmental Sciences
Aishwarya Ray, Chikere Nkwonta, Patrick Forrestal, Martin Danaher, Karl Richards, Tom O'Callaghan, Sean Hogan, Enda Cummins
Summary: The study focuses on urease inhibitor NBPT and nitrification inhibitor DCD, showing that NBPT can significantly reduce ammonia loss while raising concerns about potential risks to human health from DCD. Therefore, a comprehensive risk assessment is recommended to evaluate the potential risks from exposure to these inhibitors.
REVIEWS ON ENVIRONMENTAL HEALTH
(2021)
Article
Immunology
Bernard Vanhove, Odile Duvaux, Juliette Rousse, Pierre-Joseph Royer, Gwenaelle Evanno, Carine Ciron, Elsa Lheriteau, Laurent Vacher, Nadine Gervois, Romain Oger, Yannick Jacques, Sophie Conchon, Apolline Salama, Roberto Duchi, Irina Lagutina, Andrea Perota, Philippe Delahaut, Matthieu Ledure, Melody Paulus, Ray T. So, Chris Ka-Pun Mok, Roberto Bruzzone, Marc Bouillet, Sophie Brouard, Emanuele Cozzi, Cesare Galli, Dominique Blanchard, Jean-Marie Bach, Jean-Paul Soulillou
Summary: Heterologous polyclonal antibodies offer a potential alternative in COVID-19 treatment, but may trigger human xenogeneic antibody responses. A recent study introduced a method to produce glyco-humanized polyclonal antibodies that showed promising results in neutralizing the virus, highlighting the importance of further clinical assessment.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Food Science & Technology
Lorenzo De Colli, Karl De Ruyck, Mohamed F. Abdallah, John Finnan, Ewen Mullins, Steven Kildea, John Spink, Christopher Elliott, Martin Danaher
Summary: This study investigated the natural co-occurrence of 42 mycotoxins in unprocessed oat grains grown in Ireland, identifying the influences of different farming systems on mycotoxin production, with gluten free oats containing lower concentrations of certain toxins. Further research is needed to define optimal agronomic practices and to expand knowledge on the occurrence of various mycotoxins.
Review
Food Science & Technology
Maxime Gavage, Philippe Delahaut, Nathalie Gillard
Summary: In recent years, high-resolution mass spectrometry has become increasingly popular for food, feed, and environmental analysis. Due to its full-scan analysis and ability to screen an unlimited number of compounds in a single analysis, it is well-suited for monitoring contaminants and residues. Through relevant studies and methods, high-resolution mass spectrometry can achieve detection levels in compliance with current legislation, making it a versatile and appropriate tool for routine testing.
Article
Biochemistry & Molecular Biology
Chikere G. Nkwonta, Macdara O'Neill, Niharika Rahman, Mary Moloney, Patrick J. Forrestal, Sean A. Hogan, Karl G. Richards, Enda Cummins, Martin Danaher
Summary: A quick, robust, and sensitive method for the simultaneous determination of NBPT and NBPTo in milk was developed and validated in this study. Analysis of milk samples from a field study found very low concentrations of NBPT and NBPTo, suggesting minimal risk of residues occurring in the milk. The method was deemed fit-for-purpose for future monitoring of NBPT and NBPTo residues in milk products.
Article
Environmental Sciences
D. Mooney, K. G. Richards, M. Danaher, J. Grant, L. Gill, P-E Mellander, C. E. Coxon
Summary: A comprehensive study in the Republic of Ireland investigated the occurrence of 40 anthelmintic residues in groundwaters, revealing 17 compounds detected at 22% of sites, with albendazole and its transformation products being most frequently found. Multivariate statistical analysis identified significant source and pathway factors related to the occurrence of anthelmintics in groundwater, and temporal variations showed higher frequencies and concentrations during specific months coinciding with increased usage and meteorological events. This study provides valuable insights into the presence of anthelmintics in Irish groundwater and seasonal trends critical for assessing potential impacts of climate change.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Applied
Luisa Seyerlein, Nathalie Gillard, Philippe Delahaut, Gilles Pierret, Andreas Thomas, Mario Thevis
Summary: Research has shown that clomiphene can transfer into eggs and muscle tissue of laying hens, with detectable residues in eggs reaching peak concentrations of 10-20 µg per egg within a short period of time after oral administration. The presence of clomiphene in feed poses a potential threat to animal rearing and food safety.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Multidisciplinary Sciences
David Poirier, Jeremie Theolier, Riccardo Marega, Philippe Delahaut, Nathalie Gillard, Samuel Benrejeb Godefroy
Summary: Celiac disease is triggered by ingestion of gluten-containing cereals, and affected individuals need to adopt a gluten-free diet. Commercial immunoassays can help monitor gluten in food, but may lead to over or underestimation of gluten content.
Article
Environmental Sciences
Olivier Wilmart, Anne Legreve, Marie-Louise Scippo, Wim Reybroeck, Bruno Urbain, Dirk C. de Graaf, Pieter Spanoghe, Philippe Delahaut, Claude Saegerman
Summary: The study evaluated the risk to honey bee health posed by residues of pesticides and veterinary drugs in beeswax in Europe. Provisional action limits were proposed, and beeswax exceeding these limits should not be put on the market.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Oluwatobi Kolawole, Karl De Ruyck, Brett Greer, Julie Meneely, Fiona Doohan, Martin Danaher, Christopher Elliott
Summary: LC-MS/MS analysis of 202 oat crops grown in Ireland revealed key factors influencing mycotoxin prevalence and concentration, including farming system and previous crop. Organic farming systems and planting oats after a previous crop of grass were found to decrease contamination by type A trichothecenes.
Article
Food Science & Technology
Xinxin Zhao, Xiankun Sun, Bangcheng Lai, Rui Liu, Mangang Wu, Qingfeng Ge, Hai Yu
Summary: This research investigates the effect of different ultrasonic powers on the quality of pork meatballs. The results show that pork meatballs cooked with ultrasound at 450 W have the most uniform and smooth structures. The water retention capacity of the meatballs first increased and then decreased with increasing ultrasonic powers. The study concludes that appropriate ultrasonic power can improve the physicochemical properties and microstructure of pork meatballs, making ultrasonic technology an effective method for improving meat product quality.
Article
Food Science & Technology
J. Tholen, J. Grosse-Kleimann, G. Schulze Althoff, L. Kreienbrock, M. Upmann
Summary: This study provides an up-to-date evaluation of visual contamination on the slaughter line in a German industrial slaughterhouse. The main contamination types were intestinal contents and the affected areas were mostly small.
Article
Food Science & Technology
Dante T. Valente Jr, Ira B. Mandell, Benjamin M. Bohrer, Justice B. Dorleku, Cheryl P. Campbell, Tadeu E. Silva, Edenio Detmann, Alysson Saraiva, Manuel Juarez, Marcio S. Duarte
Summary: The objective of this study was to assess the influence of carcass traits on consumer evaluation of pork eating quality. The results showed no significant correlations between carcass traits and pork eating quality traits. Tenderness and overall acceptability were negatively correlated with cooking loss and shear force.
Article
Food Science & Technology
Honggyun Kim, Jiseon Lee, Sungmin Jeong, Suyong Lee, Geun-Pyo Hong
Summary: This study investigated the effect of high pressure pretreatment on the stability of pork loins during supercooling preservation. The results showed that high pressure pretreatment improved the stability of pork loins during supercooling preservation and reduced property changes compared to normal refrigeration.
Article
Food Science & Technology
Bulent Ekiz, P. Dilara Kecici, Y. Ziya Ograk, Hulya Yalcintan, Alper Yilmaz
Summary: The study suggests that the EUROP classification system may have different effectiveness in distinguishing carcass and meat quality in thin- and fat-tailed sheep breeds, and that varying levels of fatness and conformation classes significantly influence carcass characteristics.
Article
Food Science & Technology
Luciano Gutierrez, Roberto Lai, Giuseppe Nocella, Maria Sabbagh
Summary: Dry-aged beef provides a unique taste experience with superior qualities compared to traditional beef. This study analyzed the factors influencing consumers' intention to consume and willingness to pay for dry-aged beef, using an extended version of the Theory of Planned Behavior model that includes meat-eater identity. The results showed that meat-eater identity significantly influenced participants' intention to consume, with the most significant impact mediated by attitudes, subjective norms, and perceived behavioral control. Furthermore, the intention to consume strongly influenced the willingness to pay for dry-aged beef.
Article
Food Science & Technology
Patricia M. Ramos, Tracy L. Scheffler, Mariane Beline, Jocelyn Bodmer, David E. Gerrard, Saulo Luz Silva
Summary: The consumption of beef is expected to increase globally, which requires the use of Bos indicus cattle that are well-adapted to harsh climates. However, beef from these cattle is considered inferior due to lower tenderness values and reduced intramuscular fat content. Despite these drawbacks, the benefits of using Bos indicus genetics are significant and cannot be ignored.
Article
Food Science & Technology
Patricia Palma-Granados, Maria Munoz, Miguel A. Delgado-Gutierrez, Cristina Ovilo, Yolanda Nunez, Miguel A. Fernandez-Barrosso, Fernando Sanchez-Esquiliche, Luisa Ramirez, Juan M. Garcia-Casco
Summary: This study evaluated the effects of a set of 26 polymorphisms on premium cuts weights and meat quality in a larger population. The results suggest that these polymorphisms are associated with meat quality traits but may not have undesirable effects on carcass composition. Further research is needed to validate these findings.
Article
Food Science & Technology
Germani Adrian Munoz-Osorio, Cem Tirink, Thobela Louis Tyasi, Marco Antonio Ramirez-Bautista, Alvar Alonzo Cruz-Tamayo, Dany Alejandro Dzib-Cauich, Ricardo A. Garcia-Herrera, Alfonso J. Chay-Canul
Summary: The current study estimated carcass tissue composition in Black Belly ewe lambs using real-time ultrasound measurements and multivariate adaptive regression splines algorithms. The results showed that cold carcass weight and subcutaneous fat thickness were the most effective variables, while longissimus thoracis depth and area were the lowest variables for determining carcass composition.
Article
Food Science & Technology
Ruggero Menci, Giuseppe Luciano, Antonio Natalello, Alessandro Priolo, Fabrizio Mangano, Luisa Biondi, Marco Bella, Manuel Scerra, Massimiliano Lanza
Summary: This study aimed to assess the effect of dietary tara hydrolysable tannins on the performance and meat quality of finishing pigs. The results showed that the addition of tannins tended to reduce the content of certain fatty acids and cholesterol in meat, and delay lipid oxidation. The antioxidant and anti-inflammatory capacity of tara tannins may contribute to these positive effects.
Article
Food Science & Technology
Xiying Li, Minh Ha, Robyn D. Warner, Robert J. E. Hewitt, Darryl N. D'Souza, Frank R. Dunshea
Summary: Genetic lines have significant effects on carcass traits and pork quality. Terminal lines have lower backfat thickness, higher hardness and cohesiveness, and lower IMF content. Different muscles have different texture profiles, and collagen and IMF content greatly contribute to pork texture.
Review
Food Science & Technology
Gianni Battacone, Mondina Francesca Lunesu, Teresa Manso, Ceferina Vieira, Giuseppe Pulina, Anna Nudda
Summary: This paper examines the importance of feeding management practices in improving the nutritional properties of milk from dairy ewes and analyzes the relationship between dietary treatment of lactating ewes and the performance and meat quality of suckling lambs. The findings could be useful in developing production and communication strategies for the lamb meat industry.
Article
Food Science & Technology
B. M. Bohrer, Y. Wang, J. B. Dorleku, C. P. Campbell, I. B. Mandell
Summary: This study evaluated the pH and color of different muscles from pork carcasses and found that the longissimus thoracis had the lowest pH and the lightest and least red color. The pH and color values of the longissimus thoracis were not reliable predictors for other muscles, highlighting the importance of considering individual muscles of interest.
Article
Food Science & Technology
Maria Nobile, Luca Maria Chiesa, Francesco Arioli, Sara Panseri
Summary: This study focuses on the use of biodegradable packaging material (polylactic acid, PLA) for sliced salami as an alternative to the current material (polyethylene terephthalate, PET). The results show that the PLA packaged salami maintains its red color and stable pH throughout the shelf life, with only a slight difference in sensory markers at the end. Consumers still appreciate the product.
Article
Food Science & Technology
Wenxiang Wang, Tianliu Zhang, Lili Du, Keanning Li, Lupei Zhang, Haipeng Li, Xue Gao, Lingyang Xu, Junya Li, Huijiang Gao
Summary: This study compared different types of muscles through transcriptomic analysis and identified differences in gene expression. Several gene modules associated with muscle fiber diameter were also identified. The findings of this study provide insights for breeding to improve meat yield.
