Article
Medicine, General & Internal
In Seol Yoo, Yu-Ran Lee, Seong Wook Kang, Jinhyun Kim, Hee-Kyoung Joo, Su-Jin Yoo, Chan Keol Park, Ha-Reum Lee, Ji Ah Park, Byeong-Hwa Jeon
Summary: The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Lauren Sahakian, Ainsley M. Robinson, Linda Sahakian, Rhian Stavely, Mark R. Kelley, Kulmira Nurgali
Summary: Inflammatory bowel disease (IBD) is a chronic relapsing inflammation of the gastrointestinal tract. The prevalence of IBD is increasing worldwide, calling for the development of new treatments. APE1/Ref-1 has been identified as a potential target for IBD therapy due to its role in regulating crucial pathways in inflammatory diseases. This review discusses the current status of IBD treatments, the role of APE1/Ref-1 in intestinal inflammation, and the potential of small molecule inhibitors to modulate inflammation and oxidative stress in IBD.
Review
Biochemistry & Molecular Biology
Gabriella D. Hartman, Nathan A. Lambert-Cheatham, Mark R. Kelley, Timothy W. Corson
Summary: As global population ages, the prevalence of neovascular eye diseases is increasing, highlighting the importance of developing therapies targeting multiple pathways to alleviate the burden and risks associated with current treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Obstetrics & Gynecology
Xiuwei Wang, Huixuan Yue, Shen Li, Jin Guo, Zhen Guan, Zhiqiang Zhu, Bo Niu, Ting Zhang, Jianhua Wang
Summary: The study found significant associations between three polymorphisms of APE1/Ref-1 and NTD subtypes, suggesting that APE1/Ref-1 may be a potential genetic risk factor for encephalocele in high-risk areas of NTDs in China.
REPRODUCTIVE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arianna Barchiesi, Veronica Bazzani, Agata Jabczynska, Lukasz S. Borowski, Silke Oeljeklaus, Bettina Warscheid, Agnieszka Chacinska, Roman J. Szczesny, Carlo Vascotto
Summary: APE1 is a versatile protein involved in DNA repair, gene expression regulation, and RNA metabolism in mitochondria. Loss of APE1 leads to accumulation of damaged mitochondrial mRNA, impairing protein translation and mitochondrial respiration efficiency. The data demonstrate that APE1 plays a crucial role in maintaining mitochondrial well-being.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Analytical
Qi Kang, Xiaoyan Yang, Yumin Du, Yinxiao Qi, Zhiqiang He, Hua Xiang
Summary: In this study, a molecular gated hyperbranched rolling circle amplification (HRCA) quick sensing system was designed for rapid detection of APE1. The system exhibited rapid, simple, and sensitive APE1 detection and showed potential therapeutic applications for cancer treatment.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Anastasiia T. Davletgildeeva, Alexandra A. Kuznetsova, Darya S. Novopashina, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: AP-endonucleases are multifunctional enzymes that are required for cell viability. In this study, the effect of metal ions on the exo- and endonuclease activities of four homologous APE1-like endonucleases was analyzed. It was found that these enzymes showed similar dependence patterns on metal ions' concentrations in terms of AP-endonuclease activity. The efficiency of the 3'-5' exonuclease activity was highest in hAPE1, while the endoribonuclease activity varied among the enzymes. These findings indicate that substrate specificity and cleavage efficiency are controlled by factors external to the catalytic site.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Suravi Pramanik, Yingling Chen, Heyu Song, Irine Khutsishvili, Luis A. Marky, Sutapa Ray, Amarnath Natarajan, Pankaj K. Singh, Kishor K. Bhakat
Summary: This study identifies a novel role for the protein APE1 in regulating stable G4 formation and KRAS expression in PDAC, suggesting that G4 structures could be potential prognostic markers and therapeutic targets for PDAC.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Eun Young Bae, Hong Ryang Kil, Byeong-Hwa Jeon, Geena Kim
Summary: This study investigated the association between APE1/Ref-1 and Kawasaki disease (KD). The results showed that APE1/Ref-1 levels were significantly higher in KD patients, especially in refractory KD. APE1/Ref-1 could be a beneficial biomarker for the diagnosis and prognosis of KD.
Article
Biochemistry & Molecular Biology
Nicole M. Hoitsma, Timothy H. Click, Pratul K. Agarwal, Bret D. Freudenthal
Summary: The study provides insights into the processing of abasic ribonucleotides (rAPs) by APE1, revealing a decrease in activity compared to canonical deoxy-AP substrates. Novel contacts between rAP and the APE1 active site were identified using X-ray crystallography, showing accommodation of the rAP sugar pucker in a C3'-endo conformation. This work advances the understanding of ribonucleotide processing within genomic DNA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Cell Biology
Anastasiia T. Davletgildeeva, Alexander A. Ishchenko, Murat Saparbaev, Olga S. Fedorova, Nikita A. Kuznetsov
Summary: The study compared conformational changes of three homologous APE1-like endonucleases during their interaction with different damaged DNA substrates, outlining a model of substrate recognition by this class of enzymes. Differences in rates of DNA substrate binding did not significantly affect cleavage efficiency, indicating that enzyme-substrate complex formation is not the limiting factor for enzyme turnover; mechanisms of damage recognition and cleavage efficacy are related to fine conformational tuning inside the active site.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Huanhuan Wang, Linghao Zhang, Huaqing Sun, Shufeng Xu, Kun Li, Xin Su
Summary: In this study, we developed an inhibitory aptamer for APE1 using SELEX technology. The aptamer showed high binding affinity and could entirely inhibit the activity of APE1. These aptamers can be utilized for early cancer diagnosis and treatment, as well as a valuable tool for studying the function of APE1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yu-Ran Lee, Hee-Kyoung Joo, Eun-Ok Lee, Sungmin Kim, Hao Jin, Yeon-Hee Choi, Cuk-Seong Kim, Byeong-Hwa Jeon
Summary: The study found that 17β-estradiol is the most effective hormone in inducing APE1/Ref-1 secretion, which may occur through exosomes dependent on ER and intracellular calcium.
Review
Biochemistry & Molecular Biology
David J. Lopez, Jose A. Rodriguez, Sonia Banuelos
Summary: APE1, a crucial enzyme in DNA repair pathways, has additional functions beyond its endonuclease activity, including redox regulation of transcription factors and involvement in RNA metabolism. The N-terminal region of APE1 plays a significant role in regulating its function, particularly through interactions with nucleophosmin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kaili Long, Lili Gu, Lulu Li, Ziyu Zhang, Enjie Li, Yilan Zhang, Lingfeng He, Feiyan Pan, Zhigang Guo, Zhigang Hu
Summary: APE1 plays a critical role in NSCLC and its inhibitor NO.0449-0145 induces DNA damage and cell death, overcoming resistance to traditional chemotherapy in NSCLC cells. These findings highlight the therapeutic potential of targeting APE1 in NSCLC and developing small-molecule drugs for the treatment of other cancers.
CELL DEATH & DISEASE
(2021)
Article
Genetics & Heredity
Millie M. Georgiadis, Qiujia Chen, Jingwei Meng, Chunlu Guo, Randall Wireman, April Reed, Michael R. Vasko, Mark R. Kelley
Article
Oncology
Derek P. Logsdon, Michelle Grimard, Meihua Luo, Safi Shahda, Yanlin Jiang, Yan Tong, Zhangsheng Yu, Nicholas Zyromski, Ernestina Schipani, Fabrizio Carta, Claudiu T. Supuran, Murray Korc, Mircea Ivan, Mark R. Kelley, Melissa L. Fishel
MOLECULAR CANCER THERAPEUTICS
(2016)
Article
Oncology
Jixin Ding, Melissa L. Fishel, April M. Reed, Erin McAdams, Magdalena B. Czader, Angelo A. Cardoso, Mark R. Kelley
MOLECULAR CANCER THERAPEUTICS
(2017)
Article
Oncology
Fenil Shah, Emery Goossens, Nadia M. Atallah, Michelle Grimard, Mark R. Kelley, Melissa L. Fishel
MOLECULAR ONCOLOGY
(2017)
Editorial Material
Cell Biology
Mark R. Kelley, Jill C. Fehrenbacher
NEURAL REGENERATION RESEARCH
(2017)
Article
Neurosciences
Jill C. Fehrenbacher, Chunlu Guo, Mark R. Kelley, Michael R. Vasko
Article
Geriatrics & Gerontology
Tao Yan, Poornima Venkat, Michael Chopp, Alex Zacharek, Peng Yu, Ruizhuo Ning, Xiaoxi Qiao, Mark R. Kelley, Jieli Chen
Article
Pharmacology & Pharmacy
Sheik Pran Babu Sardar Pasha, Kamakshi Sishtla, Rania S. Sulaiman, Bomina Park, Trupti Shetty, Fenil Shah, Melissa L. Fishel, James H. Wikel, Mark R. Kelley, Timothy W. Corson
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2018)
Article
Multidisciplinary Sciences
Derek P. Logsdon, Fenil Shah, Fabrizio Carta, Claudiu T. Supuran, Malgorzata Kamocka, Max H. Jacobsen, George E. Sandusky, Mark R. Kelley, Melissa L. Fishel
SCIENTIFIC REPORTS
(2018)
Article
Cell & Tissue Engineering
Zhigang Cai, Jonathan J. Kotzin, Baskar Ramdas, Sisi Chen, Sai Nelanuthala, Lakshmi Reddy Palam, Ruchi Pandey, Raghuveer Singh Mali, Yan Liu, Mark R. Kelley, George Sandusky, Morvarid Mohseni, Adam Williams, Jorge Henao-Mejia, Reuben Kapur
Article
Chemistry, Medicinal
Richard Trilles, Dmitri Beglov, Qiujia Chen, Hongzhen He, Randall Wireman, April Reed, Spandan Chennamadhavuni, James S. Panek, Lauren E. Brown, Sandor Vajda, John A. Porco, Mark R. Kelley, Millie M. Georgiadis
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Oncology
Melissa Fishel, Hanyu Xia, Jack McGeown, David W. McIlwain, May Faek Mohamed Elbanna, Ariel A. Craft, Hristos Z. Kaimakliotis, George Sandusky, Chi Zhang, Roberto Pili, Mark R. Kelley, Travis J. Jerde
MOLECULAR CANCER THERAPEUTICS
(2019)
Article
Genetics & Heredity
Marta Codrich, Marina Comelli, Matilde Clarissa Malfatti, Catia Mio, Dilara Ayyildiz, Chi Zhang, Mark R. Kelley, Giovanni Terrosu, Carlo Em Pucillo, Gianluca Tell
Article
Oncology
Charles L. Loprinzi, Christina Lacchetti, Jonathan Bleeker, Guido Cavaletti, Cynthia Chauhan, Daniel L. Hertz, Mark R. Kelley, Antoinette Lavino, Maryam B. Lustberg, Judith A. Paice, Bryan P. Schneider, Ellen M. Lavoie Smith, Mary Lou Smith, Thomas J. Smith, Nina Wagner-Johnston, Dawn L. Hershman
JOURNAL OF CLINICAL ONCOLOGY
(2020)
Article
Pharmacology & Pharmacy
Mark R. Kelley, James H. Wikel, Chunlu Guo, Karen E. Pollok, Barbara J. Bailey, Randy Wireman, Melissa L. Fishel, Michael R. Vasko
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2016)