Article
Food Science & Technology
Alireza Ebrahimi, Gholamreza Pazuki, Mehrdad Mozaffarian, Farzaneh Ghazizadeh Ahsaie, Hamed Abedini
Summary: In this study, an aqueous two-phase system (ATPS) was used for the purification of C-phycocyanin. The Pluronic 10R5/potassium phosphate buffer system was found to be the most suitable system for separating C-phycocyanin from contaminants. Under certain conditions, the purity index of C-phycocyanin can reach 3.92 with a recovery of 90%. Additionally, using the ultrafiltration method, the purity factor can reach 5.9. Circular dichroism (CD) spectroscopy confirmed that the structure of C-phycocyanin remained intact during the purification process. Finally, 78% of the 10R5 copolymer was recovered by increasing the temperature above 57 degrees C.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Environmental Sciences
Yun Deng, Wang Xu, Qinghuai Zeng, Feiyun Sun, Fei Wang, Yanjie Li
Summary: The soil-air partition coefficients (KSA) of PBDEs and OPFRs were measured using a solid-phase fugacity meter, and the effects of temperature and relative humidity on KSA values were analyzed. An inverse relationship was found between temperature/RH and KSA values for both PBDEs and OPFRs. The study also found a significant correlation between log KSA and log KOA. The experimental KSA values differed greatly from the predicted values based on KOA. This study provides a better understanding of the behavior and fate of PBDEs and OPFRs in soil-air systems.
Article
Energy & Fuels
Karthick Murugan Palanisamy, Prakash Bhuyar, Mohd Hasbi Ab. Rahim, Natanamurugaraj Govindan, Gaanty Pragas Maniam
Summary: Palm oil mill effluent (POME) contains nutrients that support the growth of Spirulina platensis, a microalgae which has the potential to produce natural blue pigment phycocyanin for various applications. Cultivating Spirulina platensis in diluted POME medium leads to high biomass and phycocyanin yield, while the lipids extracted from the microalgae can potentially be used for biodiesel production.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Mahammed Ilyas Khazi, Chenshuo Li, Fakhra Liaqat, Przemyslaw Malec, Jian Li, Pengcheng Fu
Summary: This study evaluated two native cyanobacterial strains for phycocyanin production and found that they showed potential for valuable production. Optimal growth rates and accumulation of phycocyanin were observed under specific conditions. Increasing the nitrogen and iron concentration in the media improved growth rate and C-PC accumulation. A combined extraction protocol of freeze-thaw and ultrasonication was effective in increasing C-PC extraction efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Swati Verma, Ki-Hyun Kim
Summary: Ground water contamination by radioactive elements is a critical issue with significant threats to human health. Adsorption, especially using graphene oxide and its derivatives, is recognized as a promising method for removal due to their unique 2D structure, high surface area, and intercalated functional groups. This article provides insights into the practical applicability, preparation, and functionalization of graphene-based adsorbents for uranium removal, with guidance for future research directions in this field.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Joo Shun Tan, Sahar Abbasiliasi, Japareng Lalung, Yew Joon Tam, Paramasivam Murugan, Chee Keong Lee
Summary: This study successfully partially purified phycocyanin from Phormidium tergestinum using an ATPS system, providing a potential method for its extraction.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Han Li, Christopher M. Sims, Rui Kang, Frank Biedermann, Jeffrey A. Fagan, Benjamin S. Flavel
Summary: In this study, highly purified (>80%) left- and right-handed (6,5) single-wall carbon nanotubes (SWCNTs) were separated using aqueous two-phase extraction (ATPE), with limited experimental steps. The optimized surfactant conditions were determined, and analytical ultracentrifugation (AUC) and near-infrared photoluminescence (PL) measurements were used to examine the required conditions for surfactant wrapping and partition coefficient change (PCCCs). This study provides valuable insights into the underlying mechanisms behind surfactant-assisted SWCNT separations and promotes the future use of SWCNT enantiomers in fundamental studies and applications.
Article
Biotechnology & Applied Microbiology
Hui Suan Ng, Phei Er Kee, Hip Seng Yim, Joo Shun Tan, Yin Hui Chow, John Chi-Wei Lan
Summary: By investigating the partition behavior of GA in alcohol/salt ATPS, the optimal conditions were determined for the extraction of GA from various natural sources. This separation method can improve yield and reduce costs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
John Kiran Katari, Mohammed Rafi Uz Zama Khan, Vishal Trivedi, Debasish Das
Summary: The study presents an efficient downstream process for extracting and purifying C-phycocyanin from Spirulina sp. NCIM 5143. By optimizing the engineering strategy, a high yield of C-phycocyanin was achieved with a shorter treatment time. The purified C-phycocyanin showed potential as an antioxidant and anticancer compound with significant effects on cell migration and cell cycle arrest.
PROCESS BIOCHEMISTRY
(2023)
Article
Geochemistry & Geophysics
Qicui Jia, Shuo Zhang, Laura Lammers, Yuefei Huang, Guangqian Wang
Summary: This study reveals the relationship between the strontium partition coefficient and the calcite precipitation rate in synthetic calcite experiments, showing that this relationship varies systematically with the pH of the solution. It is hypothesized that this pH dependence is due to the involvement of HCO3- during calcite precipitation. By using an ion-by-ion calcite growth model, the hypothesis is successfully tested, explaining the pH dependence of the strontium partition coefficient and reproducing the observed relationships at different temperatures.
Article
Food Science & Technology
Yassine Benchikh, Aicha Filali, Sarra Rebai
Summary: This study successfully optimized the extraction conditions of phycocyanins from Spirulina and developed a new product of enriched natural soft beverage. The phycocyanins extract can serve as a natural food additive alternative to synthetic ones, providing antioxidant properties.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2021)
Article
Biotechnology & Applied Microbiology
Yun Ji Cho, Byung Man Lee, Youngbin Baek, Hwa Sung Shin
Summary: This study focuses on the selective extraction of chlorophyll a-pertaining photosystem polypeptides from Spirulina using aqueous two-phase extraction. The formation of a stable interfacial layer is found to be helpful in extracting the target molecules, and the purified C-Phycocyanin is proven to be safe for use.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Chemistry, Physical
Alvaro Silva Lima, Bruno Sales de Oliveira, Selesa Vanessa Shabudin, Mafalda Almeida, Mara Guadalupe Freire, Katharina Bica
Summary: This work aimed to integrate the extraction and purification of anthocyanins from grape pomace using ATPS and scaled-up CPC, with protic ionic liquids as the common solvent. Different operational conditions led to varied purification factors, with ATPS and CPC achieving significant purification of anthocyanins. The choice of optimal operational conditions depends on the specific application of anthocyanins.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Engineering, Chemical
Tania E. Sintra, Sara S. Bagagem, Farzaneh Ghazizadeh Ahsaie, Andreia Fernandes, Margarida Martins, Ines P. E. Macario, Joana L. Pereira, Fernando J. M. Goncalves, Gholamreza Pazuki, Joao A. P. Coutinho, Sonia P. M. Ventura
Summary: This study presents a sequential downstream process for extracting C-phycocyanin and chlorophyll from Anabaena cylindrica. By optimizing operational conditions, high pigment recovery rates were achieved. Additionally, purification of C-phycocyanin using polymer-based ABS led to a significant increase in purity without compromising photostability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Environmental Sciences
Jin Zhang, Kun Wang, Qitao Yi, Tao Zhang, Wenqing Shi, Xuefei Zhou
Summary: This study reveals that metal transport and partitioning in plain river networks with sedimentary resuspension are primarily controlled by suspended solids and seasonal flow regimes. The concentration of metals is associated with suspended solids in the water column and decreases from low flow to high flow, while the partitioning between particulate phase and dissolvable phases is reversed and increases from low flow to high flow. The findings provide insights into the ecological risks posed by metals in downstream lakes and highlight areas with heavy metal pollution and the transport routines of metals in river networks.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Chemical
Chetan A. Nayak, Navin K. Rastogi
DESALINATION AND WATER TREATMENT
(2010)
Article
Engineering, Chemical
Chetan A. Nayak, Navin K. Rastogi
Article
Chemistry, Applied
Chetan A. Nayak, P. Srinivas, Navin K. Rastogi
Article
Food Science & Technology
Chetan A. Nayak, Navin K. Rastogi
FOOD RESEARCH INTERNATIONAL
(2013)
Article
Food Science & Technology
Chetan A. Nayak, Navin K. Rastogi, K. S. M. S. Raghavarao
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2010)
Article
Engineering, Chemical
Chetan A. Nayak, Satya Sriram Valluri, Navin K. Rastogi
JOURNAL OF FOOD ENGINEERING
(2011)
Article
Engineering, Chemical
Chetan A. Nayak, Navin K. Rastogi
SEPARATION AND PURIFICATION TECHNOLOGY
(2010)
Article
Food Science & Technology
H. N. Pradeep, Chetan A. Nayak
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2019)
Article
Engineering, Chemical
S. Chethana, Chetan A. Nayak, K. S. M. S. Raghavarao
JOURNAL OF FOOD ENGINEERING
(2007)
Article
Chemistry, Physical
Revathy Baskaran, A. Usha Devi, Chetan A. Nayak, V. B. Kudachikar, M. N. Keshava Prakash, Maya Prakash, K. V. R. Ramana, N. K. Rastogi
RADIATION PHYSICS AND CHEMISTRY
(2007)
Article
Engineering, Chemical
Chetan A. Nayak, K. Suguna, K. Narasimhamurthy, N. K. Rastogi
JOURNAL OF FOOD ENGINEERING
(2007)
Article
Engineering, Chemical
N. K. Rastogi, K. Suguna, C. A. Nayak, K. S. M. S. Raghavarao
JOURNAL OF FOOD ENGINEERING
(2006)
Article
Engineering, Chemical
CA Nayak, K Suguna, NK Rastogi
JOURNAL OF FOOD ENGINEERING
(2006)
Article
Chemistry, Physical
CA Nayak, S Chethana, NK Rastogi, KSMS Raghavarao
RADIATION PHYSICS AND CHEMISTRY
(2006)