Article
Microbiology
K. S. Sritha, Sarita G. Bhat
Summary: This study demonstrated the potential effectiveness of a cocktail of bacteriophages in reducing Salmonella spp. contamination in different food matrices, showing significant reductions in liquid egg, milk, eggshell, and lettuce. The findings suggest the stability of phages in various food matrices, highlighting their potential as a biocontrol agent for Salmonella spp. in food safety measures.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Food Science & Technology
Hong Pan, Mei Shu, Tian-Jiao Li, Kai-Sheng Shen, Yuan-Yang Zhao, Ning-Bo Liao, Chan Zhong, Guo-Ping Wu
Summary: In this study, two lytic Salmonella phages were isolated and characterized, and they showed effective antibacterial activity against multidrug-resistant Salmonella in foods. These phages demonstrated high activity in a wide range of pH and temperature conditions. Treatment with the phages and their cocktail resulted in significant reduction of bacterial counts in lettuce and milk. These results indicate that these phages can be used as antibacterial agents to control Salmonella contamination, especially when used in combination.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Md. Ashrafudoulla, Hee Jeong Kim, Eun Her, Shanjida Shaila, Si Hong Park, Sang-Do Ha
Summary: The aim of this study was to isolate and characterize bacteriophages specific to Salmonella Thompson, evaluate their efficacy in reducing Salmonella Thompson biofilm on eggshell, and confirm the anti-biofilm activity using advanced microscopic analysis. The isolated bacteriophages CAU-STP-1 and CAU-STP-2 showed high specificity and stability at specific temperature and pH conditions. Treatment with both bacteriophages together was more effective in inhibiting Salmonella Thompson compared to using CAU-STP-1 alone. This study provides promising information for the potential development of an effective biocontrol agent against Salmonella Thomson within the food industry.
Article
Microbiology
Pawel Korzeniowski, Paulina Sliwka, Maciej Kuczkowski, Dusan Misic, Agata Milcarz, Marta Kuzminska-Bajor
Summary: In this study, bacteriophages were used to degrade biofilms formed by Salmonella enterica serovar Enteritidis strains. The phages were effective in reducing biofilm formation on various surfaces, and the phage cocktail completely eradicated Salmonella from contaminated poultry drinkers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Anisha M. Thanki, Guillaume Mignard, Robert J. Atterbury, Paul Barrow, Andrew D. Millard, Martha R. J. Clokie
Summary: Infections caused by Salmonella spp. result in millions of cases of human food poisoning every year, with a significant proportion attributed to contaminated pork products. This study demonstrates that prophylactic feeding of phage-treated feed effectively reduces Salmonella colonization in pigs, without negative impacts on the gut microbiota.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Zhongjing Lu, John Marchant, Samantha Thompson, Henry Melgarejo, Dzhuliya Ignatova, Rana Damaj, Hedy Trejo, Rodrigo Paramo, Ashley Reed, Fred Breidt, Sophia Kathariou
Summary: This study isolated 14 Salmonella phages from turkey fecal and cecal samples, among which Phi Ent exhibited a remarkably broad and unusual host range, as well as high lytic activity against multiple Salmonella serovars.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Pablo Cristobal-Cueto, Alberto Garcia-Quintanilla, Jaime Esteban, Meritxell Garcia-Quintanilla
Summary: Bacteriophages, a promising alternative in biological control, can effectively eliminate multi-drug resistant bacterial strains in the livestock industry. Their use reduces the undesirable effects of chemicals on food, improving food biocontrol. The World Health Organization estimates that around 420,000 people die annually from foodborne illnesses, highlighting the importance of enhancing food biocontrol.
Article
Food Science & Technology
Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: In this study, a lytic phage STG2 capable of reducing S. enteritidis and S. typhimurium contaminations on cabbage and various surfaces was characterized. Genomic characterization revealed that phage STG2 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing both planktonic cells and biofilms on different surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Wattana Pelyuntha, Ananya Yafa, Ruttayaporn Ngasaman, Mingkwan Yingkajorn, Kridda Chukiatsiri, Nidanut Champoochana, Kitiya Vongkamjan
Summary: The study focused on the application of phage treatment in controlling Salmonella during pre-harvest step of poultry meat production. The phage cocktail was found to be an effective biocontrol agent in reducing Salmonella in the gut of broilers.
Article
Agriculture, Dairy & Animal Science
Matias Aguilera, Eduardo Tobar-Calfucoy, Victoria Rojas-Martinez, Rodrigo Norambuena, Maria Jesus Serrano, Onix Cifuentes, Maria Sofia Zamudio, Daniel San Martin, Pabla Lara, Andrea Sabag, Marcela Zabner, Daniel Tichy, Pamela Camejo, Luis Leon, Michael Pino, Soledad Ulloa, Felipe Rojas, Christian Pieringer, Cecilia Muster, Daniel Castillo, Nicolas Ferreira, Camilo Avendano, Mauro Canaval, Hans Pieringer, Pablo Cifuentes, Nicolas Cifuentes Munoz
Summary: In this study, we isolated and characterized 47 bacteriophages that showed variable degrees of lytic activity against 258 Salmonella isolates from a commercial broiler company in Brazil. Three of these bacteriophages were selected to assemble a cocktail, which was found to be highly effective against multiple serovars of Salmonella in vitro, including under conditions that resemble the chicken gut. The cocktail also showed limited or no cross-resistance, highlighting its potential for in vivo application in commercial broiler farms.
Article
Food Science & Technology
Wattana Pelyuntha, Kitiya Vongkamjan
Summary: The study found that a combined treatment using phage and organic acid is effective in controlling Salmonella in poultry meat, with the combination not only completely killing Salmonella but also significantly reducing the number of Salmonella in contaminated chicken meat. This approach could serve as an alternative method to improve food safety in the poultry meat industry.
Article
Microbiology
Janet Y. Nale, Gurinder K. Vinner, Viviana C. Lopez, Anisha M. Thanki, Preeda Phothaworn, Parameth Thiennimitr, Angela Garcia, Manal AbuOun, Muna F. Anjum, Sunee Korbsrisate, Edouard E. Galyov, Danish J. Malik, Martha R. J. Clokie
Summary: The study found that phages can be used to control Salmonella infection in animals, and specific application methods were investigated. Prophylactic regimen was more effective than remedial regimen, showing promising potential for treating infections in pigs and poultry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Tengteng Li, Hong Chen, Jiayi Zhao, Zhenxiang Tao, Weiqing Lan, Yong Zhao, Xiaohong Sun
Summary: In this study, a novel Salmonella phage vB_SalM_SPJ41 was isolated from poultry farms in Shanghai, China. It was found that this phage could lyse multiple serotypes of antibiotic-resistant S. enterica and effectively inhibit the biofilm produced by antibiotic-resistant S. enterica. Additionally, the phage showed promising results in reducing the levels of S. Enteritidis and S. Derby in lettuce and salmon at low temperatures. Genomic analysis revealed the absence of virulence factor genes or antibiotic resistance genes in the phage.
Article
Microbiology
Ahmed Esmael, Ehab Azab, Adil A. Gobouri, Mohamed A. Nasr-Eldin, Mahmoud M. A. Moustafa, Shereen A. Mohamed, Omnia A. M. Badr, Alzahraa M. Abdelatty
Summary: Foodborne salmonellosis is a global threat, and in this study, two broad-spectrum lytic Salmonella phages, SPHG1 and SPHG3, were isolated and characterized for their ability to infect multidrug-resistant Salmonella Typhimurium. These phages showed strong lytic activity with a short lysis time and high burst sizes, and were stable under various environmental conditions. A phage cocktail containing SPHG1 and SPHG3 significantly reduced the viable count of S. Typhimurium in milk, water, and chicken breast, demonstrating their potential as biocontrol agents against foodborne salmonellosis.
Article
Microbiology
Marta Kuzminska-Bajor, Paulina Sliwka, Pawel Korzeniowski, Maciej Kuczkowski, David Saez Moreno, Anna Wozniak-Biel, Emilia Sliwinska, Krzysztof Grzymajlo
Summary: The UPWr_S134 phage cocktail has shown promising results in reducing Salmonella in broiler chickens. The phages in the cocktail remain active under various conditions encountered in the chicken gastrointestinal tract and exhibit strong stability. In mouse and chicken models, the application of the UPWr_S134 phage cocktail delayed infection symptoms and reduced the number of pathogens in internal organs compared to untreated animals.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Organic
Ana Maria Montagut, Albert Granados, Anna Ballesteros, Roser Pleixats, Montserrat Llagostera, Pilar Cortes, Rosa Maria Sebastian, Adelina Vallribera
Article
Genetics & Heredity
Miquel Sanchez-Osuna, Pilar Cortes, Montserrat Llagostera, Jordi Barbe, Ivan Erill
MICROBIAL GENOMICS
(2020)
Article
Engineering, Environmental
David Fernandez-Verdejo, Pilar Cortes, Paqui Blanquez, Ernest Marco-Urrea, Albert Guisasola
Summary: This study demonstrates that using different carbon-based cathodic electrode materials in BES can promote the growth of Dehalogenimonas and the degradation of 1,2-DCP, with the graphite brush electrode showing better performance.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xu Han, Gerard Boix, Mateusz Balcerzak, Oscar Hernando Moriones, Mary Cano-Sarabia, Pilar Cortes, Neus Bastus, Victor Puntes, Montserrat Llagostera, Inhar Imaz, Daniel Maspoch
Summary: This study reports the development of a metal-organic framework based composite for the controlled release of iodine, which exhibits antibacterial properties without causing drug resistance. The composite was successfully integrated into a polymer to create antibacterial films, which showed effective long-term release of iodine. In vitro tests confirmed the antibacterial activity of these films against gram-positive and gram-negative bacteria.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Miquel Sanchez-Osuna, Pilar Cortes, Mark Lee, Aaron T. Smith, Jordi Barbe, Ivan Erill
Summary: Lesions to DNA can threaten cell survival by compromising chromosome integrity, and the bacterial SOS response is a common transcriptional regulatory mechanism to address DNA damage. A novel family of transcriptional repressor proteins in the Bacteroidetes has been identified, orchestrating the canonical response to DNA damage in this phylum. The discovery sheds light on the origin and plasticity of the transcriptional network involved in the SOS response.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Environmental Sciences
Jesica M. Soder-Walz, Clara Torrento, Camelia Algora, Kenneth Wasmund, Pilar Cortes, Albert Soler, Teresa Vicent, Monica Rosell, Ernest Marco-Urrea
Summary: A novel Dehalobacter strain capable of transforming TCM to dichloromethane was obtained, along with its ability to transform other chlorinated compounds. Analysis of 16S rRNA genes confirmed growth of Dehalobacter with TCM and other chlorinated compounds as electron acceptors, and isotope fractionation during the transformation process was studied. These findings demonstrate a potential tool for differentiating TCM degraders in field studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Infectious Diseases
Maria Lajarin-Reinares, Eloy Pena-Rodriguez, Mariona Canellas-Santos, Elisabet Rosell-Vives, Pilar Cortes, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: Topically applied antimicrobial drugs have several advantages, but the widespread use of antibiotics has led to increasing antimicrobial resistance. Drug repurposing, such as with disulfiram, may provide an attractive alternative for treating multidrug-resistant bacteria in skin infections.
Article
Pharmacology & Pharmacy
Maria Lajarin-Reinares, Iria Naveira-Souto, Mireia Mallandrich, Joaquim Suner-Carbo, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: This study aimed to develop and optimize a mucoadhesive drug delivery system for the local administration of disulfiram. The results showed that these gels had antifungal activity against Candida albicans, Candida parapsilosis, and Nakaseomyces glabratus. The physicochemical properties and release profiles of the gels were also characterized and investigated.
Article
Immunology
Julia Lopez-Perez, Jennifer Otero, Miquel Sanchez-Osuna, Ivan Erill, Pilar Cortes, Montserrat Llagostera
Summary: This study investigates the emergence of Salmonella strains with reduced susceptibility to a cocktail of three phages in different scenarios. The results show that the mechanisms of resistance vary in different environments, but they do not significantly impair the success of Salmonella biocontrol and oral phage therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Green & Sustainable Science & Technology
David Fernandez-Verdejo, Pilar Cortes, Albert Guisasola, Paqui Blanquez, Ernest Marco-Urrea
Summary: This study investigated the sequential transformation of chloroform to acetate using bioelectrochemical systems (BESs) and syntrophic dechlorinating cultures. The results showed the advantages of combining bacteria to fully detoxify chlorinated compounds in BESs, expanding the application of this technology for treating polluted water bodies.
ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY
(2022)
Article
Immunology
Jordi Corral, Maria Perez-Varela, Miquel Sanchez-Osuna, Pilar Cortes, Jordi Barbe, Jesus Aranda
Summary: Acinetobacter baumannii is a clinically significant pathogen with increasing worldwide importance, especially due to its ability to acquire resistance genes. This bacterium can move through two types of motility: twitching and surface-associated, with strain MAR002 exhibiting both. Mutants with altered motility patterns were identified through spontaneous rifampin-resistant mutations in the rpoB gene. Transcriptomic analysis revealed deregulated genes within each motility phenotype, highlighting the key role of surface-associated motility and limited role of twitching in the pathogenicity of A. baumannii strain MAR002.
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)