Article
Engineering, Chemical
Jinye Zhang, Sihang Li, Wenjing Wang, Jianfei Pei, Jiawei Zhang, Tianli Yue, Wirote Youravong, Zhenyu Li
Summary: This study successfully developed a food functional microfiltration membrane by multi-stage modification, with polydopamine and nisin microspheres immobilized onto the membrane surface, resulting in enhanced rejection of Alicyclobacillus acidoterrestris and simultaneous exclusion and inactivation. The modified membrane also showed reliable stability under different membrane cleaning procedures.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Food Science & Technology
Hao Ding, Tiecheng Wang, Yuhan Sun, Yuxiang Zhang, Jianping Wei, Rui Cai, Chunfeng Guo, Yahong Yuan, Tianli Yue
Summary: This study used cold plasma to inactivate A. acidoterrestris, a bacterium resistant to heat treatment in fruit juice production. The inactivation efficacy was determined using the plate count method and described using a biphasic model. The study also investigated the effects of food matrix, input power, gas flow rate, and treatment time on inactivation efficacy, and analyzed the inactivation mechanism using scavenging experiments, scanning electron microscopy, and Raman spectra.
Article
Food Science & Technology
Pilar Colas-Meda, Iolanda Nicolau-Lapena, Inmaculada Vinas, Isma Neggazi, Isabel Alegre
Summary: The study evaluated the efficacy of UV-C light in reducing bacterial spores during orange juice production, showing that UV treatment on orange peel significantly reduced spore numbers, while more time was needed for the same reduction in orange juice.
Article
Chemistry, Applied
Hafida Wahia, Cunshan Zhou, Olugbenga Abiola Fakayode, Robert Amanor-Atiemoh, Long Zhang, Abdullateef Taiye Mustapha, Jin Zhang, Baoguo Xu, Rong Zhang, Haile Ma
Summary: The study showed that the use of multifrequency ultrasound technology can effectively inactivate AAT vegetative cells and spores in orange juice without negatively impacting its quality. Additionally, the production of ROS was found to have an inverse relationship with AAT inactivation.
Article
Acoustics
Akila Amir Tahi, Sergio Sousa, Khodir Madani, Cristina L. M. Silva, Fatima A. Miller
Summary: Ultrasounds are considered a good alternative technology in juice preservation, and when combined with heat treatment, it can more effectively reduce the survival of Staphylococcus aureus in orange juice compared to heat treatment alone. This study showed a synergistic effect between sonication and high temperatures, leading to improved microbial reduction and membrane damage in cells.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Chemistry, Applied
Congdi Shang, Tong Zhang, Junnan Xu, Ning Zhao, Wentao Zhang, Mingtao Fan
Summary: Contaminated Alicyclobacillus has caused significant losses in the beverage industry due to fruit juice spoilage. Understanding the growth and metabolism of Alicyclobacillus acidoterrestris (A. acidoterrestris) is crucial in controlling juice spoilage. This study systematically simulated the growth and metabolism of A. acidoterrestris in both medium and fruit juice. The results revealed that low temperature and strong acidic conditions inhibited the growth and reproduction of A. acidoterrestris, leading to improved quality of commercially available juice supplemented with A. acidoterrestris under low temperature. This research provides valuable insights for exploring additive-free methods to control juice spoilage.
Article
Food Science & Technology
Angela Aparecida Silva, Julio Cesar Polonio, Andressa Domingos Polli, Joao Arthur dos Santos Oliveira, Lilian Capelari Soares, Verci Alves Oliveira Junior, Veronica Elisa Pimenta Vicentini, Arildo Jose Braz Oliveira, Jose Eduardo Goncalves, Regina Aparecida Correia Goncalves, Joao Lucio Azevedo, Joao Alencar Pamphile, Benicio Alves Abreu Filho
Summary: The crude extract of the endophytic fungus F. flavus showed significant antibacterial activity against Alicyclobacillus acidoterrestris, especially when combined with the bacteriocin nisin, exhibiting a synergistic interaction. The extract also demonstrated greater selectivity towards bacterial cells over host cells, with observed interference in the cell membrane of A. acidoterrestris. These results suggest biotechnological potential for the crude extract in the food industry, particularly for the treatment of orange juices by controlling A. acidoterrestris.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Hafida Wahia, Long Zhang, Cunshan Zhou, Abdullateef Taiye Mustapha, Olugbenga Abiola Fakayode, Robert Amanor-Atiemoh, Haile Ma, Mokhtar Dabbour
Summary: This study investigated the effectiveness of pulsed multifrequency ultrasound in minimizing Alicyclobacillus acidoterrestris (AAT) spores and vegetative cells. It was found that dual-frequency thermosonication and ultrasonication significantly reduced AAT bacteria, with dual-frequency thermosonication exhibiting higher bactericidal efficacy. Microscopic analysis revealed ultra-structural modifications induced by sonoporation. Fourier-transform infrared spectroscopy showed major changes in the membrane fatty acids and proteins of AAT. The results suggested that pulsed multifrequency ultrasound can be an alternative to mono-frequency ultrasound in the beverage industry.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Qiuxia Fan, Cheng Liu, Zhenpeng Gao, Zhongqiu Hu, Zhouli Wang, Jianbo Xiao, Yahong Yuan, Tianli Yue
Summary: Thymoquinone (TQ) exhibited antibacterial activity against Alicyclobacillus acidoterrestris by disrupting bacterial cell morphology and membrane integrity, exerting killing effect on spores and inactivating mature biofilms. TQ shows promise as a new alternative for controlling A. acidoterrestris contamination in the juice and acid beverage industry.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Zhouli Wang, Yu Tian, Qi Wang, Tianmin Guo, Yahong Yuan, Tianli Yue, Hang Jia, Qian Ge, Zidan Zhao, Rui Cai
Summary: This study investigated the antibacterial activity and underlying mechanism of five preservatives (epsilon-polylysine, propylparaben, monocaprin, octyl gallate, and heptylparaben) against Alicyclobacillus acidoterrestris in apple juice. Results showed that all five preservatives exerted antibacterial activity through a multiple bactericidal mechanism. Monocaprin and octyl gallate had the highest antibacterial activity, with the minimum inhibitory concentration (MIC) values of 22.5 and 6.25 mg/L, respectively. Additionally, the preservatives altered the permeability of the cell membrane, leading to a leakage of intracellular electrolytes. The treatment with epsilon-polylysine, propylparaben, and monocaprin increased the leakage of intracellular protein, while propylparaben and octyl gallate reduced the levels of cellular adenosine triphosphate. Monocaprin and octyl gallate could effectively inactivate the contamination of A. acidoterrestris in apple juice without significant adverse effects on the juice quality.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Jasmine Hadj Saadoun, Alessia Levante, Martina Marrella, Valentina Bernini, Erasmo Neviani, Camilla Lazzi
Summary: The food industry needs to ensure product stability, which is often achieved through heat treatments that can eliminate microorganisms. However, this may result in a loss of nutritional and sensory value. The addition of natural antimicrobials may be a valuable option to combat microbial survival.
Article
Food Science & Technology
Yiheng Shi, Lei Zhou, Xiaowei Qu, Tianli Yue, Yahong Yuan
Summary: An effective method for identifying A. acidoterrestris using is-ELISA was developed, which is highly sensitive and specific, suitable for preventing juice spoilage.
Review
Food Science & Technology
Hafida Wahia, Olugbenga Abiola Fakayode, Abdullateef Taiye Mustapha, Cunshan Zhou, Mokhtar Dabbour
Summary: Due to their high-water activity, acidic fruits are prone to spoilage caused by microbial activity. Juice manufacturers are increasingly exploring non-thermal processing methods, such as ultrasonication, to overcome the challenges of mild heat treatment. This article reviews previous research on the inactivation and germination of Alicyclobacillus acidoterrestris in fruit juice using ultrasound and heat.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Patra Sourri, Anthoula A. Argyri, George-John E. Nychas, Chrysoula C. Tassou, Efstathios Z. Panagou
Summary: The population dynamics of A. acidoterrestris spores and vegetative cells in orange juice, treated with temperature-assisted HHP and stored in different isothermal conditions, were investigated in this study. The results showed that HHP treatment effectively reduced the spore population, and there were minimal changes in the quantity of spores and vegetative cells during storage, especially at low temperatures.
FERMENTATION-BASEL
(2022)
Article
Food Science & Technology
Yiheng Shi, Yuhan Sun, Xiaowei Qu, Lei Zhou, Zhouli Wang, Zhenpeng Gao, Zhongqiu Hu, Tianli Yue, Yahong Yuan
Summary: In this study, a highly specific monoclonal antibody was developed to detect A. acidoterrestris with great potential for preventing spoilage in the fruit juice industry. The immunoassay platform demonstrated rapid, sensitive, and selective detection capabilities, making it a robust tool for industry applications.
Article
Energy & Fuels
Luciana Teresa Dias Cappelini, Patricia Fatima Menegoci Eugenio, Juliana Vieira Alberice, Ana Carolina Urbaczek, Nilson Antonio Assuncao, Patricia Locosque Ramos, Fernando Cesar Sala, Emanuel Carrilho, Luiz Juliano, Elma Neide Vasconcelos Martins Carrilho
Summary: This study evaluated the potential of organic compost from a zoo in Brazil in providing nutrients to the soil and its impact on lettuce cultivation. The results showed that the compost-enriched soil exhibited improved quality and significantly larger plant growth, indicating the benefits of compost for soil fertilization.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Veerle W. Daniels, Jason J. Zoeller, Nick van Gastel, Kelley E. McQueeney, Salma Parvin, Danielle S. Potter, Geoffrey G. Fell, Vinicius G. Ferreira, Binyam Yilma, Rajat Gupta, Johan Spetz, Patrick D. Bhola, Jennifer E. Endress, Isaac S. Harris, Emanuel Carrilho, Kristopher A. Sarosiek, David T. Scadden, Joan S. Brugge, Anthony Letai
Summary: Cancer cells, particularly triple-negative breast cancer cells, were found to exhibit increased mitochondrial apoptotic priming and sensitivity to BH3 mimetics when treated with NAMPT inhibitors. These findings suggest a close interaction between metabolic vulnerabilities and mitochondrial apoptotic signaling pathways in some TNBC cells, providing new insights into potential targeted therapy for this subtype of breast cancer.
Review
Chemistry, Analytical
Weliton Pedro Batiston, Emanuel Carrilho
Summary: The text discusses the different performances of Linus Pauling, James Watson, and Francis Crick in the study of chemical bonds, as well as the description and challenges of proteomics analysis tools.
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lais Canniatti Brazaca, Amanda Hikari Imamura, Nathalia Oezau Gomes, Mariana Bortholazzi Almeida, Desiree Tamara Scheidt, Paulo A. Raymundo-Pereira, Osvaldo N. Oliveira Jr, Bruno Campos Janegitz, Sergio Antonio Spinola Machado, Emanuel Carrilho
Summary: This study reports the development of a low-cost immunosensor based on gold-modified screen-printed carbon electrodes for the fast and sensitive determination of S proteins from SARS-CoV and SARS-CoV-2. The immunosensor showed high selectivity, sensitivity, and a low limit of detection, allowing for direct determination of target proteins in saliva samples. The immunoplatform presented in this study has the potential for mass testing in point-of-care settings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hui Ling Ma, Laudemir Carlos Varanda, Janice Rodrigues Perussi, Emanuel Carrilho
Summary: In this study, Hypericin-loaded oil-in-water nanoemulsions (NEs) were successfully produced to enhance the solubility and bioavailability of Hypericin. The physical and biological properties of the NEs were characterized, showing significant cytotoxic effects against breast cancer cells. The results suggest that the Hypericin-loaded NEs could be a promising approach for enhancing the efficacy of Hypericin in cancer treatment.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Rosilene A. Prestes, Luiz A. Colnago, Emanuel Carrilho, Nelson R. Antoniosi Filho, Maria Isabel R. Alves
Summary: This article examines the effects of Citrus sudden death (CSD) on the fatty acid composition and presents an alternative method of diagnosing CSD using fatty acid profiles.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2022)
Article
Biochemical Research Methods
Banny S. B. Correia, Vinicius G. Ferreira, Priscila M. F. D. Piagge, Mariana B. Almeida, Nilson A. Assuncao, Joyce R. S. Raimundo, Fernando L. A. Fonseca, Emanuel Carrilho, Daniel R. Cardoso
Summary: This study used metabolomics to compare Covid-19 patients with a control group, revealing alterations in metabolite levels and identifying six metabolites that could potentially serve as prognostic markers for the disease. The metabolic changes observed suggest that SARS-CoV-2 infection disrupts the energetic system in the human body, supporting viral replication and correlating with the severity of clinical conditions in patients.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Luiz R. G. Silva, Jessica S. Stefano, Luiz O. Orzari, Lais C. Brazaca, Emanuel Carrilho, Luiz H. Marcolino-Junior, Marcio F. Bergamini, Rodrigo A. A. Munoz, Bruno C. Janegitz
Summary: A low-cost and disposable graphene polylactic (G-PLA) 3D-printed electrode modified with gold particles (AuPs) was developed to detect the cDNA of SARS-CoV-2 and creatinine, a potential biomarker for COVID-19. The study demonstrated that this approach offers a fast, simple, and cost-effective analysis.
Article
Nanoscience & Nanotechnology
Elsa M. Materon, Faustino R. Gomez, Mariana B. Almeida, Flavio M. Shimizu, Ademar Wong, Kelcilene B. R. Teodoro, Filipe S. R. Silva, Manoel J. A. Lima, Monara Kaelle S. C. Angelim, Matias E. Melendez, Nelson Porras, Pedro M. Vieira, Daniel S. Correa, Emanuel Carrilho, Osvaldo N. Oliveira Jr, Ricardo B. Azevedo, Debora Goncalves
Summary: Low-cost, instrument-free colorimetric tests were developed using plasmonic biosensors with Au nanoparticles functionalized with polyclonal antibodies. The tests showed high sensitivity and accuracy in detecting SARS-CoV-2, and could be used in dynamic light scattering and UV-vis spectroscopy. By processing the images collected from smartphone cameras with image processing and machine learning algorithms, 100% accurate COVID-19 diagnosis for saliva samples could be achieved.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Pharmacology & Pharmacy
Hui Ling Ma, Ana Carolina Urbaczek, Fayene Zeferino Ribeiro de Souza, Claudia Bernal, Janice Rodrigues Perussi, Emanuel Carrilho
Summary: This study developed a microchip to generate constant shear stress for investigating the efficacy of HyPDT on HUVECs. The microchip showed its potential in promoting the release of VEGF and inducing cell damage and death under shear stress, suggesting its importance in evaluating the therapeutic outcome in preclinical tests.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Ivana F. Rosa, Ana P. B. Pecanha, Tabata R. B. Carvalho, Leonardo S. Alexandre, Vinicius G. Ferreira, Lucas B. Doretto, Beatriz M. Souza, Rafael T. Nakajima, Patrick da Silva, Ana P. Barbosa, Leticia Gomes-de-Pontes, Camila G. Bomfim, Glaucia M. Machado-Santelli, Antonio Condino-Neto, Cristiane R. Guzzo, Jean P. S. Peron, Magaiver Andrade-Silva, Niels O. S. Camara, Anali M. B. Garnique, Renata J. Medeiros, Fausto K. Ferraris, Leonardo J. G. Barcellos, Jose D. Correia-Junior, Jorge Galindo-Villegas, Monica F. R. Machado, Angela Castoldi, Susana L. Oliveira, Camila C. Costa, Marco A. A. Belo, Giovane Galdino, German G. Sgro, Natalia F. Bueno, Silas F. Eto, Flavio P. Veras, Bianca H. V. Fernandes, Paulo R. S. Sanches, Eduardo M. Cilli, Guilherme Malafaia, Rafael H. Nobrega, Aguinaldo S. Garcez, Emanuel Carrilho, Ives Charlie-Silva
Summary: The study investigates the potential effect of red photobiomodulation (PBM) as a therapy for COVID-19. The findings suggest that inflammation is a significant feature of the disease, and PBM can help regulate the inflammatory response. Metabolomics analysis also reveals shared impacts on metabolic pathways between PBM and COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Materials Science, Biomaterials
Jessica F. Feitor, Lais C. Brazaca, Amanda M. Lima, Vinicius G. Ferreira, Giulia Kassab, Vanderlei S. Bagnato, Emanuel Carrilho, Daniel R. Cardoso
Summary: Globalization has increased concerns about disease transmission and the need for efficient drug screening methods. Organ-on-a-chip technology has emerged as a viable alternative, simulating organ characteristics and predicting drug effects accurately. However, most devices still rely on materials from the micromachining industry, raising concerns about the environmental impact of plastic waste. This critical review discusses recent advances and scalability of organ-on-a-chip technology, as well as the importance of sustainability in research and production.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Chemistry, Analytical
Marija Jozanovic, Iva Pukles, Nikola Sakac, Emanuel Carrilho, Aniko Kilar, Brunislav Matasovic, Mirela Samardzic, Mateja Budetic, Ferenc Kilar
Summary: Microchip electrophoresis is the most promising method for integrated lab-on-a-chip devices, and the use of nanomaterials in electrophoresis has opened up vast possibilities. This comprehensive review provides an overview of recent applications of nanomaterials in microchip electrophoresis, including their use as stationary and pseudostationary phases, as well as for analyte preconcentration. The review aims to highlight the increased capabilities of microchip electrophoresis and nanomaterial synergy in analytical separation methods.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Rosilene A. Prestes, Luiz A. Colnago, Emanuel Carrilho, Nelson R. Antoniosi Filho, Maria Isabel R. Alves
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2022)
Article
Engineering, Biomedical
Hui L. Ma, Wanlu Li, Mian Wang, Laudemir C. Varanda, Janice R. Perussi, Y. Shrike Zhang, Emanuel Carrilho
Summary: Advances in biomimetic three-dimensional melanoma models have great potential for drug screening and disease modeling. In this study, a GelMA-based 3D melanoma model using microemulsion was developed for evaluating the effectiveness of photodynamic therapy. The results showed that microemulsion loaded with hypericin exhibited higher phototoxicity and cell accumulation compared to free hypericin, and the cells cultured in the 3D system displayed lower susceptibility to the drug.
BIO-DESIGN AND MANUFACTURING
(2022)
Article
Food Science & Technology
Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong
Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio
Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni
Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai
Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim
Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco
Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Review
Food Science & Technology
Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse
Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley
Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis
Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi
Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
Article
Food Science & Technology
Daria Nowinski, Tomasz Czapka, Irena Maliszewska
Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2024)
