4.4 Letter

Activation state of platelets in experimental severe hemophilia A

Journal

HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
Volume 97, Issue 7, Pages 1115-1116

Publisher

FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2012.065235

Keywords

-

Categories

Ask authors/readers for more resources

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Cell Biology

Intravenous abciximab as a rescue therapy for immediate reocclusion after successful mechanical thrombectomy in acute ischemic stroke patients

Francois Delvoye, Stephane Loyau, Julien Labreuche, Guillaume Taylor, Benjamin Maier, Michel Piotin, Raphael Blanc, Simon Escalard, Lucas Di Meglio, Malek Ben Maacha, Hocine Redjem, Stanislas Smajda, Gabriele Ciccio, Solene Hebert, Candice Sabben, Martine Jandrot-Perrus, Alain Maertens De Noordhout, Mikael Mazighi, Benoit Ho-Tin-Noe, Jean-Philippe Desilles

Summary: This study investigated the use of the glycoprotein IIb/IIIa antagonist abciximab in treating immediate reocclusion after mechanical thrombectomy for acute ischemic stroke. The results showed that abciximab treatment led to higher recanalization rates without increasing bleeding complications. In vitro experiments further demonstrated that abciximab effectively limits thrombus accretion by blocking platelet aggregation.

PLATELETS (2022)

Article Cardiac & Cardiovascular Systems

Platelets and Antiplatelet Medication in COVID-19-Related Thrombotic Complications

Waltraud C. Schrottmaier, Anita Pirabe, David Pereyra, Stefan Heber, Hubert Hackl, Anna Schmuckenschlager, Laura Brunnthaler, Jonas Santol, Kerstin Kammerer, Justin Oosterlee, Erich Pawelka, Sonja M. Treiber, Abdullah O. Khan, Matthew Pugh, Marianna T. Traugott, Christian Schoergenhofer, Tamara Seitz, Mario Karolyi, Bernd Jilma, Julie Rayes, Alexander Zoufaly, Alice Assinger

Summary: Anticoagulation is associated with reduced mortality in COVID-19, but the role of antiplatelet therapy remains unclear. Adverse outcome in COVID-19 is related to dysregulation of the coagulation system, but the contribution of platelets to thrombotic complications is still uncertain.

FRONTIERS IN CARDIOVASCULAR MEDICINE (2022)

Editorial Material Hematology

It talees guts to boost platelet reactivity and inflammation

Julie Rayes

Summary: The intestinal IL-33-ST2-serotonin axis plays a key role in immune-endocrinal crosstalk during hemostasis and platelet-dependent neutrophil recruitment during inflammation.

BLOOD (2022)

Editorial Material Hematology

GPVI and collagen: the final word?

Alexandre Slater, Martine Jandrot-Perrus

Summary: The authors of this study report the structure of platelet glycoprotein VI complexed with collagen-related peptides and propose the structural basis of GPVI clustering on collagen fibers.

BLOOD (2022)

Review Hematology

Platelet-inspired nanomedicine in hemostasis thrombosis and thromboinflammation

Shruti Raghunathan, Julie Rayes, Anirban Sen Gupta

Summary: Platelets, derived from megakaryocytes, play a vital role in hemostasis and can be used as a platform for disease-targeted drug delivery. Platelet defects can lead to bleeding complications, while platelet-inspired technologies have the potential to improve therapeutic efficacy.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2022)

Article Cardiac & Cardiovascular Systems

Preferential uptake of SARS-CoV-2 by pericytes potentiates vascular damage and permeability in an organoid model of the microvasculature

Abdullah O. Khan, Jasmeet S. Reyat, Harriet Hill, Joshua H. Bourne, Martina Colicchia, Maddy L. Newby, Joel D. Allen, Max Crispin, Esther Youd, Paul G. Murray, Graham Taylor, Zania Stamataki, Alex G. Richter, Adam F. Cunningham, Matthew Pugh, Julie Rayes

Summary: This study found that the interaction between pericytes and endothelial cells is disrupted in COVID-19 infection, leading to increased microvascular endothelial permeability, which may result in thrombotic and bleeding complications.

CARDIOVASCULAR RESEARCH (2022)

Article Hematology

CLEC-2 Supports Platelet Aggregation in Mouse but not Human Blood at Arterial Shear

Joshua H. Bourne, Christopher W. Smith, Natalie J. Jooss, Ying Di, Helena C. Brown, Samantha J. Montague, Mark R. Thomas, Natalie S. Poulter, Julie Rayes, Steve P. Watson

Summary: C-type lectin-like receptor 2 (CLEC-2) is highly expressed on platelets and plays a critical role in lymphatic development. This study demonstrates that CLEC-2 supports thrombus formation at sites of inflammation without affecting hemostasis. It suggests that CLEC-2 could be a promising therapeutic target for thromboinflammatory disorders.

THROMBOSIS AND HAEMOSTASIS (2022)

Article Immunology

SARS-CoV-2 Spike- and Nucleoprotein-Specific Antibodies Induced After Vaccination or Infection Promote Classical Complement Activation

Rachel E. Lamerton, Edith Marcial-Juarez, Sian E. Faustini, Marisol Perez-Toledo, Margaret Goodall, Sian E. Jossi, Maddy L. Newby, Iain Chapple, Thomas Dietrich, Tonny Veenith, Adrian M. Shields, Lorraine Harper, Ian R. Henderson, Julie Rayes, David C. Wraith, Steve P. Watson, Max Crispin, Mark T. Drayson, Alex G. Richter, Adam F. Cunningham

Summary: This study investigates the interaction between SARS-CoV-2 antibodies and complement activation using an ELISA assay. The results suggest that the downstream effects of antibody-dependent complement activation may vary depending on the disease status and the specific antigen targeted.

FRONTIERS IN IMMUNOLOGY (2022)

Article Hematology

S100A8/A9 drives the formation of procoagulant platelets through GPIbα

Martina Colicchia, Waltraud C. Schrottmaier, Gina Perrella, Jasmeet S. Reyat, Jenefa Begum, Alexandre Slater, Joshua Price, Joanne C. Clark, Zhaogong Zhi, Megan J. Simpson, Joshua H. Bourne, Natalie S. Poulter, Abdullah O. Khan, Phillip L. R. Nicolson, Matthew Pugh, Paul Harrison, Asif J. Iqbal, George E. Rainger, Steve P. Watson, Mark R. Thomas, Nicola J. Mutch, Alice Assinger, Julie Rayes

Summary: S100A8/A9, secreted by activated myeloid cells, has antimicrobial, proinflammatory, and prothrombotic properties. Increased levels of S100A8/A9 in COVID-19 patients are associated with poor outcomes. S100A8/A9 is mainly detected in neutrophils and deposited on vessel walls in COVID-19 lungs. Platelets interact with S100A8/A9, leading to the formation of procoagulant platelets.

BLOOD (2022)

Article Biochemistry & Molecular Biology

Coagulation Factor XIIIa and Activated Protein C Activate Platelets via GPVI and PAR1

Ilaria De Simone, Constance C. F. M. J. Baaten, Martine Jandrot-Perrus, Jonathan M. Gibbins, Hugo ten Cate, Johan W. M. Heemskerk, Chris Jones, Paola E. J. van der Meijden

Summary: Platelet and coagulation activation are reciprocal processes, and factors other than thrombin or fibrin can induce platelet activation via GPVI and PAR receptors.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Hematology

A gain-of-function filamin A mutation in mouse platelets induces thrombus instability

Frederic Adam, Alexandre Kauskot, Lamia Lamrani, Jean Solarz, Christelle Soukaseum, Christelle Reperant, Cecile Denis, Hana Raslova, Jean-Philippe Rosa, Marijke Bryckaert

Summary: This study investigates the thrombotic potential of a male patient with a FLNa mutation in an in vivo model. They establish a mutant FlnA knock-in mouse model which phenocopies the patient's platelets showing increased platelet functions in vitro. However, in vivo, the mutant FlnA mice exhibit thrombus instability evidenced by increased re-bleeding and arteriolar embolies.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2022)

Article Immunology

Severity of SARS-CoV-2 infection is associated with high numbers of alveolar mast cells and their degranulation

Olga Krysko, Joshua H. Bourne, Elena Kondakova, Elena A. Galova, Katharine Whitworth, Maddy L. Newby, Claus Bachert, Harriet Hill, Max Crispin, Zania Stamataki, Adam F. Cunningham, Matthew Pugh, Abdullah O. Khan, Julie Rayes, Maria Vedunova, Dmitri V. Krysko, Alexander Brill

Summary: This study found a strong association between SARS-CoV-2 infection and activation of mast cells (MC), which is likely driven by the inflammatory response. The levels of MC proteases were increased in the serum of COVID-19 patients and correlated with disease severity. The results suggest that MC protease levels could predict the course of the disease and that including MC-stabilizing drugs in the treatment scheme might be beneficial for severe COVID-19 patients.

FRONTIERS IN IMMUNOLOGY (2022)

Article Oncology

Human Bone Marrow Organoids for Disease Modeling, Discovery, and Validation of Therapeutic Targets in Hematologic Malignancies

Abdullah O. Khan, Antonio Rodriguez-Romera, Jasmeet S. Reyat, Aude-Anais Olijnik, Michela Colombo, Guanlin Wang, Wei Xiong Wen, Nikolaos Sousos, Lauren C. Murphy, Beata Grygielska, Gina Perrella, Christopher B. Mahony, Rebecca E. Ling, Natalina E. Elliott, Christina Simoglou Karali, Andrew P. Stone, Samuel Kemble, Emily A. Cutler, Adele K. Fielding, Adam P. Croft, David Bassett, Gowsihan Poologasundarampillai, Anindita Roy, Sarah Gooding, Julie Rayes, Kellie R. Machlus, Bethan Psaila

Summary: This study presents a human bone marrow organoid that can support the growth and survival of primary cells from patients with myeloid and lymphoid blood cancers. This organoid model allows for mechanistic studies of blood cancers within their microenvironment and serves as an ex vivo tool for prioritizing new therapeutics.

CANCER DISCOVERY (2023)

Review Hematology

Basic Mechanisms of Hemolysis-Associated Thrombo-Inflammation and Immune Dysregulation

Jordan D. D. Dimitrov, Lubka T. T. Roumenina, Gina Perrella, Julie Rayes

Summary: Regardless of the cause, hemolytic diseases are associated with thrombosis, inflammation, and immune dysregulation, resulting in organ damage and poor outcomes. Hemolysis not only leads to anemia and loss of the anti-inflammatory functions of red blood cells, but also releases damage-associated molecular patterns, such as ADP, hemoglobin, and heme, which trigger a hyperinflammatory and hypercoagulable state through various receptors and signaling pathways. Extracellular free heme, in particular, can activate platelets, endothelial cells, innate cells, as well as the coagulation and complement cascades, thereby promoting thrombo-inflammatory events. This review discusses the main mechanisms by which hemolysis, especially heme, drives this thrombo-inflammatory environment and explores the impact of hemolysis on the host response to secondary infections.

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2023)

Article Hematology

Efficacy of platelet-inspired hemostatic nanoparticles on bleeding in von Willebrand disease murine models

Stephanie Roullet, Norman Luc, Julie Rayes, Jean Solarz, Dante Disharoon, Andrew Ditto, Emily Gahagan, Christa Pawlowski, Thibaud Sefiane, Frederic Adam, Caterina Casari, Olivier D. Christophe, Michael Bruckman, Peter J. Lenting, Anirban Sen Gupta, Cecile Denis

Summary: The lack of innovation in von Willebrand disease (VWD) is due to the complexity and heterogeneity of the disease and a lack of recognition of the impact of bleeding symptoms experienced by patients. This study proposes a new approach using synthetic platelet (SP) nanoparticles for the treatment of VWD-2B and severe VWD. The results show that SP can improve thrombus formation and reduce blood loss in VWD-2B and VWF-KO mice.

BLOOD (2023)

No Data Available