Article
Hematology
Cansu Koyunlar, Emanuele Gioacchino, Disha Vadgama, Hans de Looper, Joke Zink, Mariette N. D. ter Borg, Remco Hoogenboezem, Marije Havermans, Mathijs A. Sanders, Eric Bindels, Elaine Dzierzak, Ivo P. Touw, Emma de Pater
Summary: This study demonstrates the crucial role of Gata2 in the regulation of hematopoietic stem cell (HSC) development and function. Gata2 haploinsufficiency affects the transcriptional activation of HSCs and repression of endothelial cell identity, resulting in incomplete maturation of HSCs. Moreover, hematopoietic-specific induction of Gfi1b expression can restore HSC production in gata2b-null zebrafish embryos.
Article
Multidisciplinary Sciences
Alessandro Moro, Anne van Nifterick, Ruud F. Toonen, Matthijs Verhage
Summary: This study identified dynamins as organizers of dense-core vesicle fusion sites in mammalian neurons, playing a crucial role in the release of neuropeptides and neuromodulators. Genetic or pharmacological inactivation of dynamins strongly impaired dense-core vesicle exocytosis, while synaptic vesicle exocytosis remained unaffected. The study concluded that mammalian dynamins regulate dense-core vesicle fusion sites by modulating the balance between fusogenic and non-fusogenic syntaxin-1, promoting its availability for SNARE complex formation and dense-core vesicle exocytosis.
Article
Cell Biology
Marcos A. Carpio, Robert E. Means, Allison L. Brill, Alva Sainz, Barbara E. Ehrlich, Samuel G. Katz
Summary: BOK plays a crucial role in regulating the contact between the ER and mitochondria, which is essential for apoptosis. Lack of BOK results in decreased proximity between the ER and mitochondria, altered protein composition of MAMs, and ultimately affects calcium transfer and apoptosis.
Article
Hematology
Michela Faleschini, Nicole Papa, Marie-Christine Morel-Kopp, Caterina Marconi, Tania Giangregorio, Federica Melazzini, Valeria Bozzi, Marco Seri, Patrizia Noris, Alessandro Pecci, Anna Savoia, Roberta Bottega
Summary: A novel GFI1B variant was identified in this study, which causes exon 9 skipping and overexpression of a shorter p32 isoform. The clinical data of patients with different GFI1B variants leading to the same effect on p32 expression were reported and the potential role of GFI1B in carcinogenesis regulation was investigated.
Article
Chemistry, Multidisciplinary
Jose M. Palomo
Summary: Artificial enzymes based on protein structure modification have recently seen significant advancements, with successful synthesis using multidisciplinary strategies. However, the challenge remains in creating artificial enzymes with multiple active sites, potentially opening up new directions for enzymatic tools in sustainable chemistry. Current technologies for designing artificial enzymes with two or more active sites are limited, highlighting the need for further research in this area.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Shaohui Wang, Xueying Song, Alex Rajewski, Chintda Santiskulvong, Homayon Ghiasi
Summary: The establishment of latent herpes simplex virus (HSV) infection involves the presence of latency-associated transcript (LAT) in various cells, including dendritic cells, neurons, and innate lymphoid cells. This indicates that HSV-1 can establish a quiescent/latent infection in nonneuronal cells, enhancing its survival in the host.
Article
Cell Biology
Albert J. H. Suurmeijer, Arjen H. G. Cleven, Cristina R. Antonescu, Lauren A. Duckworth, Karen J. Fritchie, Steven D. Billings, Josephine K. Dermawan
Summary: This study describes three cases of soft tissue tumors that have EWSR1::GFI1B gene fusions, which resemble soft tissue angiofibroma in morphology. These tumors present as unencapsulated infiltrative nodules composed of bland fibroblastic spindle cells and a branching vascular network. Targeted RNA sequencing identified gene fusions between EWSR1 exons 1-7 and GFI1B exons 6-11 or 7-11 in all three cases. There were no local recurrences or distant metastases during the limited follow-up period. Overall significance: 8 points.
Article
Endocrinology & Metabolism
Wang-Ren Qiu, Meng-Yue Guan, Qian-Kun Wang, Li-Liang Lou, Xuan Xiao
Summary: This study constructed a model for predicting pupylation proteins using functional domain GO annotation and Word Embedding for feature extraction. Random Under-sampling and Synthetic Minority Over-sampling Technique were used to balance the dataset, and Extreme Gradient Boosting algorithm was applied for prediction. The results showed high accuracy and good performance of the model.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Infectious Diseases
Thulasika Senthakumaran, Aina E. F. Moen, Tone M. M. Tannaes, Alexander Endres, Stephan A. A. Brackmann, Trine B. B. Rounge, Vahid Bemanian, Hege S. S. Tunsjo
Summary: This study analyzed the microbial composition in patients with colorectal cancer, adenomatous polyps, and healthy controls. The results showed that oral biofilm-associated bacteria, such as Fusobacterium, Gemella, and Parvimonas, were enriched in cancer patients. In addition, another group of bacteria was identified in both cancer and polyp patients, suggesting their potential role in polyp development and early stages of colorectal cancer.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Oncology
Longlong Liu, Pradeep Kumar Patnana, Xiaoqing Xie, Daria Frank, Subbaiah Chary Nimmagadda, Minhua Su, Donghua Zhang, Thorsten Koenig, Frank Rosenbauer, Marie Liebmann, Luisa Klotz, Wendan Xu, Jan Vorwerk, Felix Neumann, Jana Huve, Andreas Unger, Jurgen Gunther Okun, Bertram Opalka, Cyrus Khandanpour
Summary: Recent studies have revealed the important role of transcription factor GFI1B in metabolic regulation during hematopoiesis and leukemia development. Gfi1b deletion leads to activation of mitochondrial respiration and alteration of energy metabolism towards oxidative phosphorylation (OXPHOS). Gfi1b also epigenetically regulates multiple genes involved in fatty acid oxidation (FAO). The correlation between Gfi1b expression level and metabolic phenotype is influenced by genetic variations in AML cells. Inhibition of FAO or OXPHOS significantly hinders leukemia progression, and Gfi1b-deficient AML cells are more sensitive to drugs targeting OXPHOS and FAO, suggesting new potential therapeutic strategies.
Article
Immunology
Andrea Kosta, Abdelilah Mekhloufi, Lorenzo Lucantonio, Alessandra Zingoni, Alessandra Soriani, Marco Cippitelli, Angela Gismondi, Francesca Fazio, Maria Teresa Petrucci, Angela Santoni, Helena Stabile, Cinzia Fionda
Summary: This study evaluated the role of the GAS6/TAM signaling pathway in the regulation of NKG2D ligand expression and NK cell recognition of MM. The results showed that GAS6, MERTK, and AXL depletion led to downregulation of MICA and inhibition of NKG2D-mediated NK cell degranulation. GAS6 derived from bone marrow stromal cells also increased MICA expression in MM cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maaike G. J. M. van Bergen, Rinske van Oorschot, Saskia M. Bergevoet, Aniek O. de Graaf, Evelyn L. R. T. M. Tonnissen, Ellen Stevens-Linders, Kornelia Neveling, Pascal W. T. C. Jansen, Marijke P. A. Baltissen, Michiel Vermeulen, Amit Mandoli, Joost H. A. Martens, Frank Preijers, Joop H. Jansen, Bert A. van der Reijden
Summary: The genomic locus 8 kb downstream of the GFI1B transcription factor is associated with clonal hematopoiesis and myeloproliferative neoplasms, with a polymorphism rs621940-G in particular. However, studies show that rs621940-G does not affect GFI1B expression, autorepression, or growth of immature myeloid cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Chemistry, Physical
Liang Tan, Jiamei Zhu, Xiaodong He, Min Zhou, Shuangquan Zhang
Summary: Understanding the mechanism of toluene absorption by phosphonium ionic liquids (PILs) is crucial for the removal of volatile organic compounds. Quantum chemical calculations revealed that interactions between PILs and toluene are mainly driven by hydrogen bonds, C-H··π bond interactions, and electrostatic attraction. The results indicated that PILs have great potential for toluene absorption, with the cationic structure playing a key role in providing sufficient space for accommodating toluene.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Geochemistry & Geophysics
Cheng Mei, Sylvain Barbot, Yunzhong Jia, Wei Wu
Summary: This article discusses the impact of frictional sliding stability on the spectrum of fault slip, exploring the controls of fault stability and the dependence of frictional properties on load-point velocity through experiments and theoretical considerations. It suggests that in addition to the stiffness ratio of the loading system, another parameter ratio related to velocity and state dependence plays a role in controlling faulting and rupture dynamics. Variations of these two non-dimensional parameters may help explain the wide range of rupture styles and recurrence patterns observed in nature.
EARTH AND PLANETARY SCIENCE LETTERS
(2022)
Article
Immunology
Yuyang Hou, Meng Zhang, Qiannan Jiang, Yuping Yang, Jiang Liu, Ke Yuan, Zheng Sun, Xiuxiang Liu
Summary: We used a newly developed ultra-sensitive metagenomic sequencing method, 2bRAD-M, to investigate the microbial signatures of central nervous system infections in neonates. By applying this method, we identified potential biomarkers that have significantly different relative abundance based on cerebrospinal fluid examination results, which can aid in the diagnosis of neonatal bacterial meningitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Emiliana Eusebio-Ponce, Francisco Javier Candel, Robert Paulino-Ramirez, Irene Serrano-Garcia, Eduardo Anguita
Summary: This study analyzed the seroprevalence and trends of HTLV-1/2 among blood donors in Santo Domingo, Dominican Republic, finding a period prevalence of 0.26% and a marked predominance of replacement donation. The study showed a relatively low and steady trend of HTLV-1/2 seroprevalence in the region during the studied period.
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2021)
Article
Infectious Diseases
Ignacio Callejas Caballero, Marta Illan Ramos, Arantxa Berzosa Sanchez, Eduardo Anguita, Jose Tomas Ramos Amador
Summary: Severe neutropenia in infants and children, although usually benign, can potentially lead to life-threatening infections. Autoimmune neutropenia is a rare hematological disorder characterized by the destruction of neutrophils induced by autoantibodies, often triggered by viral infections. Conservative management may suffice for cases with a benign course of the disease.
BMC INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Julia Gonzalez-Rincon, Jose A. Garcia-Vela, Sagrario Gomez, Belen Fernandez-Cuevas, Sara Nova-Gurumeta, Nuria Perez-Sanz, Miguel Alcoceba, Marcos Gonzalez, Eduardo Anguita, Javier Lopez-Jimenez, Eva Gonzalez-Barca, Lucrecia Yanez, Ernesto Perez-Persona, Javier de la Serna, Miguel Fernandez-Zarzoso, Guillermo Deben, Francisco J. Penalver, Maria C. Fernandez, Jaime Perez de Oteyza, M. angeles Andreu, M. angeles Ruiz-Guinaldo, Raquel Paz-Arias, M. Dolores Garcia-Malo, Valle Recasens, Rosa Collado, Raul Cordoba, Belen Navarro-Matilla, Margarita Sanchez-Beato, Jose A. Garcia-Marco
Summary: Chronic Lymphocytic Leukemia (CLL) is the most common leukemia in Western countries, known for its variable clinical course influenced by mutational status of genes. Studies have identified recurrent somatic mutations in genes related to cell cycle, DNA repair, RNA metabolism, and splicing. Results of this study suggest that, except for TP53 gene mutations, the mutational status of other genes does not seem to affect the positive outcomes of rituximab maintenance therapy in CLL patients after frontline FCR treatment.
Article
Medicine, General & Internal
Silvia Marce, Blanca Xicoy, Olga Garcia, Marta Cabezon, Natalia Estrada, Patricia Velez, Concepcion Boque, Miguel Sagues, Anna Angona, Raul Teruel-Montoya, Francisca Ferrer-Marin, Paula Amat, Juan Carlos Hernandez-Boluda, Mariana M. Ibarra, Eduardo Anguita, Montserrat Cortes, Andres Fernandez-Ruiz, Sandra Fontanals, Lurdes Zamora
Summary: The study found that the expression of e14a2 or e13a2 transcripts in chronic myeloid leukemia (CML) patients does not affect treatment outcomes. Specifically, e14a2 transcripts are associated with better molecular responses in the first 6 months of treatment, while e13a2 is linked to improved long-term overall survival.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Rafael Diaz de la Guardia, Talia Velasco-Hernandez, Francisco Gutierrez-Aguera, Heleia Roca-Ho, Oscar Molina, Cesar Nombela-Arrieta, Alex Bataller, Jose Luis Fuster, Eduardo Anguita, Susana Vives, Lurdes Zamora, Josep Nomdedeu, Maria Teresa Gomez-Casares, Manuel Ramirez-Orellana, Helene Lapillonne, Veronica Ramos-Mejia, Juan Carlos Rodriguez-Manzaneque, Clara Bueno, Belen Lopez-Millan, Pablo Menendez
Summary: AML is a highly heterogeneous disease, and patient stratification based on risk is crucial for treatment decisions. Using NSGS mice, researchers found that engraftment levels were mostly independent of risk group, with HR AML patients showing significantly superior engraftment compared to FR or IR patients. These findings provide a valuable platform for testing targeted therapies in risk-stratified AML patient samples.
Article
Cell Biology
Candela Barettino, Alvaro Ballesteros-Gonzalez, Andres Aylon, Xavier Soler-Sanchis, Leticia Orti, Selene Diaz, Isabel Reillo, Francisco Garcia-Garcia, Francisco Jose Iborra, Cary Lai, Nathalie Dehorter, Xavier Leinekugel, Nuria Flames, Isabel Del Pino
Summary: This study investigates the role of the tyrosine kinase receptor ErbB4 in the formation and function of serotonergic circuits by disrupting gene expression specific to serotonergic subsets. It shows that ErbB4 expression in a subset of serotonergic neurons is necessary for axonal arborization to the forebrain, but not for innervation of other targets. Additionally, ErbB4 deficiency in serotonergic neurons leads to specific behavioral deficits in memory processing, involving aversive or social components.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Eduardo Anguita, Alberto Chaparro, Francisco Javier Candel, Carlos Ramos-Acosta, Neus Martinez-Micaelo, Nuria Amigo, Maria Jose Torrejon, Guillermo Llopis-Garcia, Maria del Mar Suarez-Cadenas, Mayra Matesanz, Juan Gonzalez del Castillo, Francisco Javier Martin-Sanchez
Summary: This study aimed to identify blood biomarkers related to mitochondrial and cell metabolism in older patients with acute and stable HFpEF. The results showed that patients in the decompensated phase had increased mitochondrial superoxide production and mass, lower mitochondrial DNA copy number and LDHB expression, and higher lactate level compared to the stable phase. The stable phase was characterized by a sharp reduction in formate level. The findings highlighted the relationship between HFpEF and aging and suggested potential therapeutic targets for monitoring and treatment of patients.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Oncology
Belen Lopez-Millan, Paula Costales, Francisco Gutierrez-Aguera, Rafael Diaz de la Guardia, Heleia Roca-Ho, Meritxell Vinyoles, Alba Rubio-Gayarre, Remi Safi, Julio Castano, Paola Alejandra Romecin, Manuel Ramirez-Orellana, Eduardo Anguita, Irmela Jeremias, Lurdes Zamora, Juan Carlos Rodriguez-Manzaneque, Clara Bueno, Francisco Moris, Pablo Menendez
Summary: The study found that EC-70124 is a promising and safe agent for the treatment of FLT3-ITDMUT AML, exhibiting robust and specific antileukemia activity while sparing healthy hematopoietic cells.
Article
Multidisciplinary Sciences
Ricardo Sanchez, Sara Dorado, Yanira Ruiz-Heredia, Alejandro Martin-Munoz, Juan Manuel Rosa-Rosa, Jordi Ribera, Olga Garcia, Ana Jimenez-Ubieto, Gonzalo Carreno-Tarragona, Maria Linares, Laura Rufian, Alexandra Juarez, Jaime Carrillo, Maria Jose Espino, Mercedes Caceres, Sara Exposito, Beatriz Cuevas, Raul Vanegas, Luis Felipe Casado, Anna Torrent, Lurdes Zamora, Santiago Mercadal, Rosa Coll, Marta Cervera, Mireia Morgades, Jose Angel Hernandez-Rivas, Pilar Bravo, Cristina Seri, Eduardo Anguita, Eva Barragan, Claudia Sargas, Francisca Ferrer-Marin, Jorge Sanchez-Calero, Julian Sevilla, Elena Ruiz, Lucia Villalon, Maria del Mar Herraez, Rosalia Riaza, Elena Magro, Juan Luis Steegman, Chongwu Wang, Paula de Toledo, Valentin Garcia-Gutierrez, Rosa Ayala, Josep-Maria Ribera, Santiago Barrio, Joaquin Martinez-Lopez
Summary: In this study, a novel DNA-based next-generation sequencing (NGS) method was developed for the detection and monitoring of BCR::ABL1 kinase domain (KD) mutations in chronic myeloid leukemia (CML) and B-cell precursor acute lymphoblastic leukemia (ALL) Philadelphia (Ph)-positive patients. This method showed high sensitivity and specificity, and was able to detect mutations even in patients with low levels of disease.
SCIENTIFIC REPORTS
(2022)
Article
Pathology
Chloe L. Fisher, Richard Dillon, Eduardo Anguita, Deborah J. Morris-Rosendahl, Ali R. Awan
Summary: This study presents a novel method for genomic rearrangement detection based on third-generation long-read sequencing. The method overcomes time and cost issues and demonstrates its utility in patients with acute myeloid leukemia. It not only detects expected rearrangements, but also discovers novel translocations.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Endocrinology & Metabolism
Ines Amich, Eduardo Anguita, Silvia Escribano-Serrat, Cristina Alvarez, Diego Rodriguez-Munoz, Veronica Garcia, Rocio Bello, Jose Alberto Pena-Pedrosa, Neus Martinez-Micaelo, Nuria Amigo, Pablo Ortiz, Maria Jose Torrejon, Lisardo Bosca, Javier Martin-Sanchez, Ana Aranda, Susana Alemany
Summary: Metabolic reprogramming is important in fighting COVID-19 infection, and thyroid hormones play a key role in regulating metabolism. This study compared euthyroid patients and those with hypothyroidism treated with levothyroxine (LT4) in terms of biochemical, metabolomic, cytokine, and clinical parameters. No significant differences were found between the two groups, except for TSH and ferritin levels in the ward and TSH and FT4 levels in the ICU. Age was negatively correlated with FT3, and patients with low FT3 and older age had worse prognosis and higher levels of IL-6 and IL-10. A metabolomic cluster indicative of a high ketogenic profile was identified in non-critical hospitalized patients with low FT3 levels.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ruben Gil-Redondo, Ricardo Conde, Maider Bizkarguenaga, Chiara Bruzzone, Ana Lain, Beatriz Gonzalez-Valle, Milagros Iriberri, Carlos Ramos-Acosta, Eduardo Anguita, Juan Ignacio Arriaga Lariz, Pedro Pablo Espana Yandiola, Miguel Angel Moran, Mario Ernesto Jimenez-Mercado, Leire Egia-Mendikute, Maria Luisa Seco, Hartmut Schaefer, Claire Cannet, Manfred Spraul, Asis Palazon, Nieves Embade, Shelly C. Lu, Julien Wist, Jeremy K. Nicholson, Jose M. Mato, Oscar Millet
Summary: This study investigated the natural history of COVID-19 disease at the molecular level and characterized the metabolic and immunological phenoreversion in hospitalized severe patients and non-hospitalized recovered patients. The findings suggest that acute patients experience metabolic and immunological dysregulation, resulting in a slower recovery time, while non-hospitalized recovered patients do not show any associated metabolic or immune alterations.
Article
Microbiology
Salvador Meseguer, Mari-Paz Rubio, Begona Lainez, Beatriz Perez-Benavente, Raul Perez-Moraga, Sergio Romera-Giner, Francisco Garcia-Garcia, Olalla Martinez-Macias, Antonio Cremades, Francisco J. Iborra, Oscar Candelas-Rivera, Fernando Almazan, Enric Esplugues
Summary: It has been found that levels of SERINC5 are reduced in COVID-19 patients, and the repression of SERINC5 expression may be caused by viral non-coding small RNAs (svRNAs).
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ricardo Pires das Neves, Monica Chagoyen, Antonio Martinez-Lorente, Carlos Iniguez, Ana Calatrava, Juana Calabuig, Francisco J. Iborra
Summary: This study reveals the importance of thiol groups from cysteines in cellular response to ROS and their role in signaling. Different subcellular compartments have different amounts of these thiol groups, with the nucleolus having the highest concentration. These findings provide insights into the differential sensitivity of cells to ROS.
Letter
Biochemistry & Molecular Biology
Francisco J. Iborra, Cristina Marti, Virtu Calabuig-Navarro, Petros Papadopoulos, Salvador Meseguer, Pedro M. Iborra, Francisco Garcia, Antonio Martinez-Lorente, Fernando Almazan, Juana Calabuig
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)