Article
Obstetrics & Gynecology
Su Zhou, Yueyue Xi, Yingying Chen, Tong Wu, Wei Yan, Milu Li, Meng Wu, Aiyue Luo, Wei Shen, Tao Xiang, Shixuan Wang
Summary: This study reveals the role of wild-type p53-induced phosphatase 1 (WIP1) in the regulation of primordial follicle development, showing that inhibiting WIP1 phosphatase accelerates primordial follicle loss via the p53-BAX-caspase-3 pathway.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Agriculture, Dairy & Animal Science
Shunshun Han, Jianping Wang, Can Cui, Chunlin Yu, Yao Zhang, Diyan Li, Menggen Ma, Huarui Du, Xiaosong Jiang, Qing Zhu, Chaowu Yang, Huadong Yin
Summary: This study explores the regulatory molecular mechanisms of follicular atresia in chickens and reveals the role of autophagy and apoptosis in this process. It also highlights the importance of fibromodulin in regulating autophagy and provides insights into broodiness and poultry production improvement.
Article
Cell Biology
Tuo Zhang, Tianhe Ren, Huan Lin, Yuntong Tong, Jixian Zhang, Jie Nie, Yingjie Zhu, Yiting Wang, Bangming Jin, Chunlin Zhang, Tengxiang Chen, Meina He
Summary: ASH1L is an important epigenetic molecule that controls oocyte apoptosis during meiotic prophase I in mice. It regulates the capacity of the primordial follicle pool and plays a role in DNA double-strand break repair. Its expression also affects p63 and p-CHK2.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Su Zhou, Yueyue Xi, Yingying Chen, Fangfang Fu, Wei Yan, Milu Li, Yaling Wu, Aiyue Luo, Ya Li, Shixuan Wang
Summary: This study revealed that WIP1 expression is significantly lower in atretic follicle granulosa cells and appropriate WIP1 expression is essential for healthy follicular development. Inhibiting WIP1 activity accelerates ovarian aging by promoting follicular atresia and primordial follicle activation, leading to decreased ovarian reserve.
Article
Biochemistry & Molecular Biology
Malgorzata J. McEvoy, Emilia Sinderewicz, Leo Creedon, Marion McAfee, Agnieszka W. Jonczyk, Katarzyna K. Piotrowska-Tomala, Dariusz J. Skarzynski
Summary: This study investigates newly discovered mechanisms of atresia and cell death processes in bovine ovarian follicles, finding that necroptosis is an alternative mechanism responsible for the death of bovine granulosa and theca cells. Analysis of mRNA expression of RIPK1 and RIPK3 supports this conclusion, highlighting the importance of both apoptosis and necroptosis in follicular cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Quynh-Nhu Nguyen, Nadeen Zerafa, Jock K. Findlay, Martha Hickey, Karla J. Hutt
Summary: The research found that mice lacking the apoptosis gene Puma retained 100% of their follicles 24 hours after chemotherapy treatment and showed efficient repair of DNA damage 8 hours post-treatment. Investigation into DNA repair pathways revealed induction of the homologous recombination pathway in damaged cell types, while non-homologous end joining played an insignificant role in primordial follicle oocytes.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
L. Cacciottola, A. Camboni, A. Cernogoraz, J. Donnez, M. M. Dolmans
Summary: The size of the ovarian follicle pool is regulated by both apoptosis and autophagy. Apoptosis is responsible for the physiological decline of the follicle pool over time and increases in the event of prior chemotherapy. Autophagy plays a major role in the diminution of the follicle pool before puberty.
HUMAN REPRODUCTION
(2023)
Article
Reproductive Biology
Jing Xu, Mary B. Zelinski
Summary: In vitro follicle development (IVFD) is a valuable model for understanding folliculogenesis, screening ovarian toxicity, and preserving fertility and species. This review discusses the culture systems used in IVFD across various species and the improvements made in follicle growth and oocyte maturation through optimization of culture conditions. The evaluation of oocyte quality following IVFD, including epigenetic and genetic profile, maturation, embryonic development, and pregnancy outcomes, is summarized. The review also highlights the limitations and knowledge gaps in IVFD for proper oocyte development. This information contributes to the goal of producing competent oocytes with genetic integrity for successful embryonic development and healthy offspring.
BIOLOGY OF REPRODUCTION
(2022)
Article
Environmental Sciences
Si-Cheng Zhao, Zhi-Ran Xu, Chang-Long Xu, Quan-Kuo He, Guang-Ming Yang, Yan-Ping Li, Yu-Shen Luo, Hai-Long Wang, Zhong-Quan Qi, Yu Liu
Summary: Exposure to nickel sulfate negatively affects ovarian homeostasis and oocyte quality, triggering ovarian fibrosis and inflammation, and causing structural and functional disorders of mitochondria.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Obstetrics & Gynecology
Y. Zhou, S. Richard, N. J. Batchelor, D. E. Oorschot, G. M. Anderson, M. W. Pankhurst
Summary: The present findings suggest that AMH-mediated follicle atresia only occurs in early follicles before they become sensitive to FSH. Additionally, the study found that AMH overexpression leads to increased apoptosis rates of granulosa cells and oocytes in the ovaries. This indicates that the role of AMH is not to preserve ovarian reserve, but to prevent excessive follicle accumulation.
HUMAN REPRODUCTION
(2022)
Article
Reproductive Biology
Michal Andrzej Kosior, Riccardo Esposito, Natascia Cocchia, Federica Piscopo, Valentina Longobardi, Nunzio Antonio Cacciola, Giorgio Antonio Presicce, Giuseppe Campanile, Hilde Aardema, Bianca Gasparrini
Summary: The aim of this study was to evaluate the seasonal effect on the metabolomic profile of the ovarian follicle in Italian Mediterranean buffalo and identify potential strategies to improve oocyte competence during the non-breeding season. Analysis of follicular fluid, follicular cells, cumulus cells, and oocytes revealed seasonal differences in metabolite content, suggesting alterations in metabolic pathways during the non-breeding season. Pathway enrichment analysis identified metabolites linked to glutathione, energy generation, amino acid metabolism, and phospholipid biosynthesis. The identification of positive and negative competence markers in the follicular fluid provides a basis for optimizing the follicular environment and improving oocyte competence during the non-breeding season.
Article
Environmental Sciences
Wei Song, Ang Li, Qian-Qian Sha, Shao-Yuan Liu, Yong Zhou, Chang-Yin Zhou, Xue Zhang, Xiao-Zhen Li, Jia-Xin Jiang, Fei Li, Chao Li, Heide Schatten, Xiang-Hong Ou, Qing-Yuan Sun
Summary: This study found that maternal exposure to 4-vinylcyclohexene diepoxide (VCD) during pregnancy significantly affects the fertility and health of mice offspring. It reduces litter size and ovarian reserve, while increasing the occurrence of microtia in F1 mice. Mechanistically, VCD inhibits meiotic prophase I progression and leads to increased expression of DNA damage markers. RAD51 and DMC1 are extensively recruited to repair DNA double strand breaks in the oocytes of VCD-exposed offspring.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Endocrinology & Metabolism
Guangxin Li, Jingkai Gu, Xiaomei Zhou, Ting Wu, Xian Li, Renwu Hua, Zhuo Hai, Yuan Xiao, Jiaping Su, Willian S. B. Yeung, Kui Liu, Chenxi Guo, Tianren Wang
Summary: Chemotherapy often leads to ovarian damage, but the role of mitochondria in this process is unknown. Using a mouse model, this study investigated mitochondrial homeostasis in oocytes and found that the oocyte-specific deletion of Clpp impaired both fertility and mitochondrial function. Furthermore, treatment with the chemotherapeutic drug CTX exacerbated the damage, leading to decreased oocyte competence and folliculogenesis. Overall, Clpp is essential for oocyte competence and its deficiency worsened cyclophosphamide induced ovarian damage.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Reproductive Biology
Qiaochu Wang, Karla J. Hutt
Summary: Cisplatin can decrease mitochondrial membrane potential in oocytes, leading to cell death. Five weeks after treatment, the number of mature oocytes harvested from cisplatin treated mice was significantly lower, but the surviving oocytes had normal mitochondrial parameters.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Environmental Sciences
Yang Yang, Guohao Wang, Yaya Li, Jiabao Hu, Yajun Wang, Zhen Tao
Summary: This study provides detailed cellular morphology of follicular atresia in silver pomfret and identifies stage-specific genes using high-throughput RNA sequencing. The downregulation of genes in cytokine-cytokine receptor interaction suggests that somatic cells may directly induce follicle atresia. The study also reveals the involvement of immune cells in the process of follicle atresia.
FRONTIERS IN MARINE SCIENCE
(2022)
