Journal
GUT AND LIVER
Volume 8, Issue 5, Pages 508-518Publisher
EDITORIAL OFFICE GUT & LIVER
DOI: 10.5009/gnl13237
Keywords
Sox9; Cdx2-transgenic mice; Stem cell marker
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Funding
- Japan Society for the Promotion of Science [22114002]
- KEIRIN RACE
- [21590793]
- [21590794]
- Grants-in-Aid for Scientific Research [22114002, 23590926] Funding Source: KAKEN
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Background/Aims: Doublecortin and CaM kinase-like-1 (DCAMKL1) is a marker of stem cells expressed predominantly in the crypt base in the intestine. However, DCAMKL1-positive cells have been shown to be differentiated tuft cells rather than quiescent progenitors. Tuft cells are the only epithelial cells that express cyclooxygenase 2 (COX-2) in the normal intestinal epithelium. We previously generated Cdx2-transgenic mice as model mice for intestinal metaplasia and gastric carcinoma. In the current study, we investigated the association between COX-2 and DCAMKL1 in gastric carcinoma. Methods: We examined the association between COX-2 and DCAMKL1 expression in gastric carcinomas in clinical samples (early gastric well-differentiated adenocarcinoma) and Cdx2-transgenic mice; and the DCAMKL1-transgenic mouse stomach using immunohistochemistry and quantitative real-time polimerase chain reaction. Results: The COX-2-expressing cells were scattered, not diffusely expressed, in gastric carcinomas from humans and Cdx2-transgenic mice. DCAMKL1-positive cells were also scattered in the gastric carcinomas, indicating that tuft cells could still be present in gastric carcinoma. COX-2 was expressed in DCAMKL1-positive tuft cells in Cdx2- and DCAMKL1-transgenic mouse stomachs, whereas the Sox9 transcription factor was ubiquitously expressed in gastric carcinomas, including COX-2-positive cells. Conclusions: COX-2 is expressed in DCAMKL1-expressing quiescent tuft cells in gastric carcinoma.
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