Journal
GREEN CHEMISTRY
Volume 14, Issue 3, Pages 734-740Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2gc16428k
Keywords
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Funding
- Fundacao para a Ciencia e a Tecnologia [SFRH/BD/37830/2007]
- CAPES [551223/2010-3]
- PIBIC from FAPITEC
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The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C(8)mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.
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