Article
Biochemistry & Molecular Biology
Meng Qiao, Junjie Zhang, Jingmin Li, Lu Xing, Xue Zhou, Yunchang Xie, Xing Zhang
Summary: This study successfully constructed an expression system and immobilized the enzymes, obtaining high expression levels and good water solubility for hyaluronic acid synthase and chondroitin synthase. The use of metal-organic frameworks as carriers efficiently catalyzed the synthesis of hyaluronic acid and chondroitin, with good stability and reusability.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Ao Dong, Chengzhi Liu, Xiaoting Hua, Yunsong Yu, Yan Guo, Dongshu Wang, Xiankai Liu, Huan Chen, Hengliang Wang, Li Zhu
Summary: This study analyzed the properties of surface polysaccharides and glycosyltransferases in E. coli through data mining. The majority of polysaccharides were composed of D-hexopyranose, and the linkages between monosaccharides were mainly alpha-1,3 and beta-1,3 bonds. A database was constructed to facilitate diagnosis and typing of E. coli.
Article
Biochemistry & Molecular Biology
Anindita Roy, Yuma Miyai, Alessandro Rossi, Krishna Paraswar, Umesh R. Desai, Yukio Saijoh, Balagurunathan Kuberan
Summary: The study presents a novel approach to produce low molecular weight heparosan and heparosan oligosaccharides by genetically engineering non-pathogenic E. coli strains, offering a promising solution to reduce reliance on animal sources for heparin production.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Multidisciplinary Sciences
Ke Xia, James T. Hagan, Li Fu, Brian S. Sheetz, Somdatta Bhattacharya, Fuming Zhang, Jason R. Dwyer, Robert J. Linhardt
Summary: The application of solid-state nanopore devices for analyzing synthetic heparan sulfate GAG chains is feasible, with clear differences between signals generated by different GAGs detected through machine learning and data analysis. These findings suggest that ultrasensitive GAG analysis could be achieved using SS nanopore detection and well-characterized molecular training sets.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Johanna J. Kenyon, Anastasiya A. Kasimova, Anastasiya N. Sviridova, Anna M. Shpirt, Mikhail M. Shneider, Yuliya Mikhaylova, Andrei A. Shelenkov, Anastasiya Popova, Andrei Perepelov, Alexander S. Shashkov, Andrei S. Dmitrenok, Alexander O. Chizov, Yuriy A. Knirel
Summary: A novel KL144 capsular polysaccharide biosynthesis gene cluster was identified, and its structure was analyzed. Comparison of different gene clusters revealed shared genes and discussed enzymes that may influence the formation of glycosidic linkages in the structures.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Marianna Barros Silva, Livia de Lourdes de Sousa Pinto, Luiz Henrique Medeiros, Airton Araujo Souza Jr, Suely Ferreira Chavante, Luciana Guimaraes Alves Filgueira, Rafael Barros Gomes Camara, Guilherme Lanzi Sassaki, Hugo Alexandre Oliveira Rocha, Giulianna Paiva Viana Andrade
Summary: Oreochromis niloticus (tilapia) is a commonly cultivated fish species, and its farming generates organic waste. In this study, tilapia viscera were processed to obtain chondroitin sulfate (CST), a biomolecule that showed potential as an anti-inflammatory drug. The CST obtained from tilapia waste significantly reduced leukocyte migration in an acute peritonitis model, indicating its pharmacological activity.
Review
Biotechnology & Applied Microbiology
Zi-Xu Liu, Si-Ling Huang, Jin Hou, Xue-Ping Guo, Feng-Shan Wang, Ju-Zheng Sheng
Summary: Valuable polysaccharides are typically produced through fermentation using wild-type or metabolically-engineered microbial strains, with high-throughput screening techniques being used to identify cells with high levels of polysaccharide synthesis. Strategies such as fluorescence labeling, fluorescence quenching, and biosensors are effective for cell sorting in industrial strains and pathway optimization for polysaccharide synthesis. Additionally, high-throughput screening using individual whole cells is critical in the discovery and evolution of enzymes involved in polysaccharide biosynthesis, with these enzymes and their mutants being valuable catalysts for saccharide synthesis.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Riya Banerjee, K. Jayaram Kumar, John F. Kennedy
Summary: This article provides an overview of recent developments in polysaccharides and their use in medical administration, emphasizing their excellent characteristics for medicine. Acidic polysaccharides, such as Pectin, Xanthan gum, Carrageenan, Alginate, and Glycosaminoglycan, are being researched as potential drug delivery systems. However, the structural activity correlations between different acidic polysaccharides and their combination effects are often overlooked in research, limiting their scope of use. This review aims to explore these correlations and expand the potential applications of acidic polysaccharides in drug delivery systems.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Peng He, Xing Zhang, Ke Xia, Dixy E. Green, Sultan Baytas, Yongmei Xu, Truong Pham, Jian Liu, Fuming Zhang, Andrew Almond, Robert J. Linhardt, Paul L. DeAngelis
Summary: Complex carbohydrates play important roles in organisms, including structure, energy, and communication. This study developed sulfur-linked polysaccharides with potentially altered conformational states, which are resistant to digestion by the important enzyme heparanase. These polysaccharides have the potential to be used in cancer therapeutics and protein/sugar interaction modulators.
NATURE COMMUNICATIONS
(2022)
Review
Plant Sciences
Jordan D. Julian, Olga A. Zabotina
Summary: This review discusses the composition of plant cell walls and the biosynthesis process of xyloglucan. It focuses on the research of xyloglucan in Arabidopsis thaliana, Oryza sativa, and Solanum lycopersicum, and also explores the recent advances in understanding the glycosyltransferases involved in this complex process.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jacqueline J. Ehrlich, Richard M. Weerts, Sayane Shome, Alan T. Culbertson, Richard B. Honzatko, Robert L. Jernigan, Olga A. Zabotina
Summary: Glycosyltransferases (GTs) are enzymes that add sugars to various acceptor substrates, and it has been found that some plant XXTs do not exhibit high substrate specificity, showing significant promiscuity in their in vitro reactions, which provides a new perspective for studying substrate specificity of GTs.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Chemistry, Physical
Jian-Qun Deng, Zhen Lu, Juan Liu, Yan Zhao, Xu-Ben Hou, Xue-Ping Guo, Wen-Jie Jiang, Feng-Shan Wang, Ju-Zheng Sheng
Summary: This study presents a biocatalytic approach for the size-controlled synthesis of heparosan oligosaccharides using two single-function glycosyltransferases. By screening and modifying enzymes, the researchers successfully synthesized size-defined heparosan oligosaccharides with minimized byproduct formation.
CATALYSIS SCIENCE & TECHNOLOGY
(2022)
Article
Plant Sciences
Kristen A. Engle, Robert A. Amos, Jeong-Yeh Yang, John Glushka, Melani A. Atmodjo, Li Tan, Chin Huang, Kelley W. Moremen, Debra Mohnen
Summary: Homogalacturonan (HG) is a crucial pectic glycan in plant growth, development, and defense against pathogens, synthesized by members of the GALACTURONOSYLTRANSFERASE (GAUT) family. Different GAUT members exhibit varying levels of HG:GalAT activity, with GAUT13 and GAUT14 showing unique de novo HG synthesis capabilities.
Article
Chemistry, Applied
Malgorzata Sulewska, Monika Berger, Manuela Damerow, David Schwarzer, Falk F. R. Buettner, Andrea Bethe, Manuel H. Taft, Hans Bakker, Martina Muehlenhoff, Rita Gerardy-Schahn, Bernard Priem, Timm Fiebig
Summary: In this study, we utilized the enzyme repertoire of E. coli K5 and the E. coli K5-specific bacteriophage φK5B to synthesize and depolymerize heparosan. We studied the elongation mechanism of E. coli K5 heparosan polymerases KfiA and KfiC using a fluorescently labeled heparosan nonamer as a priming acceptor, and found that the enzymes act in a distributive manner, producing heparosan of low dispersity. We also identified the tailspike protein KflB of φK5B as heparosan lyase and characterized its depolymerization mechanism.
CARBOHYDRATE POLYMERS
(2023)
Article
Microbiology
Rebecca A. Wilkes, Jacob Waldbauer, Ludmilla Aristilde
Summary: This study elucidates the metabolic mechanisms facilitating gluconeogenic fast-growth phenotypes in Pseudomonas putida and Comamonas testosteroni, two Proteobacteria with distinct metabolic networks. P. putida relies on a cyclic connection between the Entner-Doudoroff and Embden-Meyerhoff-Parnas pathways, while C. testosteroni bypasses the oxidative pentose phosphate pathway during gluconeogenic growth. Both species deplete protein investment into connected glycolysis pathways to minimize futile carbon cycling and favor gluconeogenic metabolism.
Article
Biochemistry & Molecular Biology
Sumit Bhattacharyya, Kemal Solakyildirim, Zhenqing Zhang, Mei Ling Chen, Robert J. Linhardt, Joanne K. Tobacman
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2010)
Review
Biochemical Research Methods
Bo Yang, Kemal Solakyildirim, Yuqing Chang, Robert J. Linhardt
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2011)
Article
Biochemical Research Methods
Fuming Zhang, Bo Yang, Mellisa Ly, Kemal Solakyildirim, Zhongping Xiao, Zhenyu Wang, Julie M. Beaudet, Amanda Y. Torelli, Jonathan S. Dordick, Robert J. Linhardt
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2011)
Article
Biochemical Research Methods
Tatiana N. Laremore, Mellisa Ly, Kemal Solakyildirim, Dmitri V. Zagorevski, Robert J. Linhardt
ANALYTICAL BIOCHEMISTRY
(2010)
Article
Chemistry, Multidisciplinary
Seok Joon Kwon, Kyung Bok Lee, Kemal Solakyildirim, Sayaka Masuko, Mellisa Ly, Fuming Zhang, Lingyun Li, Jonathan S. Dordick, Robert J. Linhardt
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2012)
Article
Chemistry, Applied
Chao Cai, Kemal Solakyildirim, Bo Yang, Julie M. Beaudet, Amanda Weyers, Robert J. Linhardt, Fuming Zhang
CARBOHYDRATE POLYMERS
(2012)
Article
Biochemical Research Methods
Meredith M. Dinges, Kemal Solakyildirim, Cynthia K. Larive
Article
Chemistry, Analytical
Sibel Irmak, Kemal Solakyildirim, Arif Hesenov, Oktay Erbatur
JOURNAL OF ANALYTICAL CHEMISTRY
(2010)
Correction
Biochemistry & Molecular Biology
Xue Zhao, Bo Yang, Kemal Solakyildirim, Eun Ji Joo, Toshihiko Toida, Kyohei Higashi, Robert J. Linhardt, Lingyun Li
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Chemistry, Medicinal
Julie M. Beaudet, Amanda Weyers, Kemal Solakyildirim, Bo Yang, Majde Takieddin, Shaker Mousa, Fuming Zhang, Robert J. Linhardt
JOURNAL OF PHARMACEUTICAL SCIENCES
(2011)
Article
Chemistry, Multidisciplinary
Kemal Solakyildirim, Daryl N. Bulloch, Cynthia K. Larive
MAGNETIC RESONANCE IN CHEMISTRY
(2014)
Review
Biochemical Research Methods
Kemal Solakyildirim
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Mariana Barboza, Kemal Solakyildirim, Trina A. Knotts, Jonathan Luke, Melanie G. Gareau, Helen E. Raybould, Carlito B. Lebrilla
Summary: This study utilized a novel technology to analyze the N-glycome of different brain regions, revealing distinct expression profiles of glycans in functional brain areas, laying the foundation for studying the specific roles of N-glycans in brain health and disease. By studying the cell membrane N-glycome, it demonstrated the spatial distribution of glycan variations in different brain regions, providing insights into the complex glyco-architecture of the brain.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Spectroscopy
Kemal Solakyildirim, Lingyun Li, Robert J. Linhardt
MASS SPECTROMETRY LETTERS
(2018)