Article
Oncology
Laura Miro, Julia Lopez, Pedro E. Guerrero, Neus Martinez-Bosch, Noemi Manero-Ruperez, Mireia Moreno, M. Rosa Ortiz, Esther Llop, Pilar Navarro, Rosa Peracaula
Summary: This study evaluated the potential of a sialyltransferase inhibitor to reduce tumor sialoglycans and revert the malignant phenotype in pancreatic cancer. The inhibitor treatment decreased sialic acid levels and weakened the tumor's growth and migration capabilities in both human pancreatic cancer cells and mouse models. Furthermore, it increased immune cell infiltration. These findings suggest that inhibiting cell sialyltransferases could be a novel therapeutic strategy for pancreatic cancer.
Article
Biochemistry & Molecular Biology
Seonghun Kim, Jinhyuk Lee, Doo-Byoung Oh, Ohsuk Kwon
Summary: The invertebrate sialyltransferase cST3Gal-I from the sea squirt Ciona savignyi shows a preference for O-glycans as substrates, effectively transferring Neu5Ac to core 1 O-glycan compared to its human orthologue. Structural modeling suggests that the active sites of cST3Gal-I are similar to a mammalian enzyme, porcine ST3Gal-I, indicating a potential ancestral preference for O-glycans in C. savignyi.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Oncology
Thomas Kolben, Lena Mueller, Sarah Meister, Lucia Keilmann, Christina Buschmann, Fabian Trillsch, Alexander Burges, Bastian Czogalla, Sophie Mittler, Elisa Schmoeckel, Stefanie Corradini, Sven Mahner, Udo Jeschke, Mirjana Kessler, Susanne Beyer
Summary: This study is first to examine the expression of SLeX, SLeA, and LeY in EC, serving as potential additional prognostic markers for the future. Further studies are needed to understand the underlying mechanisms.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2022)
Article
Chemistry, Analytical
Cheng Wang, Xiangjun Wang, Jiayu Lu, Di Wei, Nan Zhou, Youjia Li, Yuanji Wang, Huaizhen He
Summary: In this study, a fluorescence analysis method based on a DBF probe was established to detect sLeX levels in urine samples of prostate cancer patients. The method showed rapid and sensitive screening capabilities, allowing for early non-invasive screening, disease development monitoring, and prognostic detection of prostate cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Medicine, Research & Experimental
Ranim Al Saoud, Amar Hamrouni, Adi Idris, Walaa K. Mousa, Tareq Abu Izneid
Summary: Metastasis is a major cause of cancer-related deaths, posing significant health and economic burdens. Hypersialylation, characterized by excessive sialylated glycans on tumor cells, enables metastasis by repelling and detaching cells from the original tumor. Sialylation is mediated by sialyltransferases (STs), and upregulation of STs leads to increased tumor hypersialylation, ultimately allowing immune evasion.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Endocrinology & Metabolism
Liming Yu, Jun Peng, Chieko Mineo
Summary: Sialylation is a common modification in eukaryotic cells that plays a key role in various biological processes. It regulates protein-protein interactions, intracellular localization, vesicular trafficking, and signal transduction. Sialylation of lipoproteins is closely related to lipoprotein metabolism and atherogenesis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anne Bordron, Marie Morel, Cristina Bagacean, Maryvonne Dueymes, Pierre Pochard, Anne Harduin-Lepers, Christophe Jamin, Jacques-Olivier Pers
Summary: Sialylation plays a crucial role in autoimmunity, with hyposialylation often leading to inflammation and organ damage. A better understanding of this mechanism will help improve treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Rita Matos, Kaori L. Fonseca, Stefan Mereiter, Ana Raquel Maceiras, Joana Gomes, Cristina Vilaplana, Fatima Gartner, Pedro N. S. Rodrigues, Celso A. Reis, Margarida Saraiva, Ana Magalhaes
Summary: The study reveals an increased expression of the sialylated glycan structure Sialyl-Lewis X (SLeX) in lung epithelium upon M. tuberculosis infection, accompanied by altered tissue transcriptomic signature. This suggests that glycosylation-involved molecular mechanisms may modulate host response to M. tuberculosis infection, potentially shaping TB disease outcome.
Article
Chemistry, Multidisciplinary
Zechariah Thompson, Alexander J. Steed, Jack Millot, Yinghua Chen, J. A. Cowan
Summary: This study reports the use of metallopeptide-based scaffolds for sugar recognition and catalysis. The metallopeptide showed preference for specific carbohydrates and was able to remove antigens from the surface of leukemia cells. These metallopeptides may have potential therapeutic applications as artificial metalloglycosidases.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Physical
Xiaoju Zheng, Lin Zhu, Tianlu Li, Wenjia Xu, Dongke Liu, Juzheng Sheng, Hongzhi Cao, Yikang Shi, Fengshan Wang
Summary: Glycosylation provides a solution for modifying peptide drugs, improving stability and receptor selectivity. The study introduces a enzymatic modular assembly strategy for the synthesis of active glycopeptides, which significantly increases the half-lives of the peptides in human serum.
Article
Virology
Yue Kang, Zhi-Tong Mai, Lee-Fong Yau, Run-Feng Li, Tian-Tian Tong, Chun-Guang Yang, Ka-Man Chan, Zhi-Hong Jiang, Yutao Wang, Zi-Feng Yang, Jing-Rong Wang
Summary: This study revealed that the antiviral activity of ARB may be due to the inhibition of ST and the resulting destruction of SA receptors in host cells. ST inhibition has been proposed as a novel approach for antiviral treatment, and several compounds are currently being explored. ARB is the first antiviral drug on the market to possess ST inhibiting function. This study provides crucial evidence for the clinical usage of ARB and suggests it as a lead compound for the development of host-targeting antiviral drugs.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei Xiong, Wenxin Liu, Shogo Nishida, Daichi Komiyama, Wei Liu, Jotaro Hirakawa, Hiroto Kawashima
Summary: Asthma is an allergic disease characterized by severe leukocyte infiltration in the lungs, which can be significantly suppressed by the anti-sLe(x) monoclonal antibody F2, leading to reduced eosinophil infiltration, proliferation, serum IgE levels, as well as levels of Th2 cytokines and chemokines. The study suggests that sLe(x) carbohydrate antigen could be a novel therapeutic target for allergic asthma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ying Wang, Weie Zhao, Si Mei, Panyu Chen, Tsz-Ying Leung, Cheuk-Lun Lee, William S. B. Yeung, Jian-Ping Ou, Xiaoyan Liang, Philip C. N. Chiu
Summary: The protein C1orf56 was found to be a SLeX-binding protein on capacitated spermatozoa, playing a potential role in the acrosome reaction following sperm-egg binding. Clinical study showed an association between C1orf56 expression in the acrosome-reacted population and fertilization rate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
William H. D. Bowles, Tracey M. Gloster
Summary: Sialidases and sialyltransferases play essential roles in various organisms, including humans, in recognition and cell function. Inhibitors targeting these enzymes have been used in treating diseases such as cancer, but there is an ongoing need for novel potent and specific inhibitors to combat resistance.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Oncology
Makoto Tanio, Akifumi Muramoto, Hitomi Hoshino, Masataka Murahashi, Yoshiaki Imamura, Osamu Yokoyama, Motohiro Kobayashi
Summary: The study assessed the impact of sLe(x) and sLe(a), as well as E-selectin ligand carbohydrates, on metastasis and prognosis in ccRCC.
Membrane expression of HECA-452-positive sLe(x)/sLe(a) glycans correlated with shortened progression-free and overall survival.
Expression of functional E-selectin ligand carbohydrates showed a more significant correlation with poor prognosis in ccRCC patients than HECA-452 positivity.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2021)
Review
Biochemistry & Molecular Biology
Wanqing Li, John B. McArthur, Xi Chen
CARBOHYDRATE RESEARCH
(2019)
Article
Medicine, General & Internal
Juliette Rousse, Apolline Salama, Shani Leviatan Ben-Arye, Petra Hruba, Janka Slatinska, Gwenaelle Evanno, Odile Duvaux, Dominique Blanchard, Hai Yu, Xi Chen, Jean-Marie Bach, Vered Padler-Karavani, Ondrej Viklicky, Jean-Paul Soulillou
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Biochemistry & Molecular Biology
Melissa M. Matthews, John B. McArthur, Yanhong Li, Hai Yu, Xi Chen, Andrew J. Fisher
Article
Biochemistry & Molecular Biology
Yanhong Li, Riyao Li, Hai Yu, Xue Sheng, Jing Wang, Andrew J. Fisher, Xi Chen
Article
Food Science & Technology
Valerie Weinborn, Yanhong Li, Ishita M. Shah, Hai Yu, David C. Dallas, J. Bruce German, David A. Mills, Xi Chen, Daniela Barile
INTERNATIONAL DAIRY JOURNAL
(2020)
Article
Chemistry, Physical
Jiali Zhang, Hai Yu, Bradley Harris, Yunbo Zheng, Umit Celik, Lan Na, Roland Faller, Xi Chen, Dominik R. Haudenschild, Gang-yu Liu
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Virology
Karen N. Barnard, Brynn K. Alford-Lawrence, David W. Buchholz, Brian R. Wasik, Justin R. LaClair, Hai Yu, Rebekah Honce, Stefan Ruhl, Petar Pajic, Erin K. Daugherity, Xi Chen, Stacey L. Schultz-Cherry, Hector C. Aguilar, Ajit Varki, Colin R. Parrish
JOURNAL OF VIROLOGY
(2020)
Article
Virology
Runxia Liu, Chithra Sreenivasan, Hai Yu, Zizhang Sheng, Simon J. Newkirk, Wenfeng An, David F. Smith, Xi Chen, Dan Wang, Feng Li
Article
Chemistry, Physical
Riyao Li, Hai Yu, Saddam M. Muthana, Daron Freedberg, Xi Chen
Article
Microbiology
Tri Nguyen, Sohyoung Lee, Yi-An Yang, Changhwan Ahn, Ji Hyun Sim, Tiffany G. Kei, Karen N. Barnard, Hai Yu, Shawn K. Millano, Xi Chen, Colin R. Parrish, Jeongmin Song
Article
Chemistry, Physical
Lan Na, Hai Yu, John B. McArthur, Tamashree Ghosh, Thomas Asbell, Xi Chen
Article
Oncology
Ron Amon, Ronit Rosenfeld, Shahar Perlmutter, Oliver C. Grant, Sharon Yehuda, Aliza Borenstein-Katz, Ron Alcalay, Tal Marshanski, Hai Yu, Ron Diskin, Robert J. Woods, Xi Chen, Vered Padler-Karavani
Article
Biochemistry & Molecular Biology
Libo Zhang, Hai Yu, Yuanyuan Bai, Bijoyananda Mishra, Xiaoxiao Yang, Jing Wang, Evan B. Yu, Riyao Li, Xi Chen
Summary: Carbohydrate-protein conjugates have diverse applications, from vaccines to high-throughput assays. A process combining chemoenzymatic synthesis, squarate linker conjugation, and neoglycoprotein immobilization on fluorescent magnetic beads was developed for a suspension multiplex array platform. This platform allowed for analysis of glycan and GBP interactions, providing useful insights into human galectins' multivalent glycan-binding properties.
Article
Biochemistry & Molecular Biology
Wanqing Li, Marcos D. Battistel, Hannah Reeves, Lisa Oh, Hai Yu, Xi Chen, Lee-Ping Wang, Daron Freedberg
Article
Chemistry, Organic
John B. McArthur, Abhishek Santra, Wanqing Li, Anoopjit S. Kooner, Ziqi Liu, Hai Yu, Xi Chen
ORGANIC & BIOMOLECULAR CHEMISTRY
(2020)