Article
Chemistry, Physical
Rylee Wander, Andrea M. Kaminski, Zhangjie Wang, Eduardo Stancanelli, Yongmei Xu, Vijayakanth Pagadala, Jine Li, Juno M. Krahn, Truong Quang Pham, Jian Liu, Lars C. Pedersen
Summary: The study presents the crystal structures of 3-OST-5 with substrates and PAP, revealing its substrate specificity and anti-factor Xa activity. The enzyme prefers to sulfate a 6-O-sulfo glucosamine saccharide that is surrounded by glucuronic acid. Additionally, it was found that substrate specificity is not only determined by the side chains of amino acid residues in the active site, but also by the conformational flexibility of 2-O-sulfated iduronic acid in the saccharide substrates.
Article
Biochemistry & Molecular Biology
Federico M. Ruiz, Francisco J. Medrano, Anna-Kristin Ludwig, Herbert Kaltner, Nadezhda V. Shilova, Nicolai V. Bovin, Hans-Joachim Gabius, Antonio Romero
Summary: This study presents the first crystallographic information on the N-terminal extension of rat galectin-5, revealing the structured organization around the central Trp77 of the carbohydrate recognition domain. The ligand-bound structure demonstrates a shift in the amino acid alignment, potentially acting as a molecular switch for new contacts in this region.
Article
Immunology
Juliane Guenther, Sebastian Peter Galuska
Summary: Galectins are a diverse family of carbohydrate-binding proteins found in vertebrates, and their evolutionary history has been revealed through genomic analyses. The study highlights the origin and evolution of galectin family members in different animal groups, including primitive Bilateria and jawed vertebrates. Additionally, the research identifies a previously neglected galectin-related protein in Gnathostomata.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Chemistry, Medicinal
Hongqun Liu, Sang-Youn Hwang, Samuel S. S. Lee
Summary: Cirrhotic cardiomyopathy refers to abnormal cardiac function in cirrhosis patients without primary cardiac disease. Its pathogenesis involves two pathways: abnormal liver structure and function due to cirrhosis and synthetic liver failure, and portal hypertension accompanied by leaky gut and systemic inflammation. Current therapies for cirrhotic cardiomyopathy are limited due to baseline vasodilation in cirrhotic patients, making new treatment modalities necessary. Galectin-3 inhibitors, such as modified citrus pectin, N-acetyllactosamine, TD139, and GB0139, show potential therapeutic benefits with their anti-apoptotic, anti-oxidative, and anti-inflammatory effects.
Article
Biochemistry & Molecular Biology
Kristyna Slamova, Jakub Cerveny, Zuzana Meszaros, Tereza Friede, David Vrbata, Vladimir Kren, Pavla Bojarova
Summary: Gal-4 is a carbohydrate-binding lectin expressed in the epithelial cells of the gastrointestinal tract, regulating cell proliferation, apoptosis, adhesion, and migration by cross-linking glycans on cell membranes or extracellular matrix components. Its altered expression in tumor tissue is associated with various cancers, and this study provides insights into its binding preferences and the influence of multivalency. These findings may contribute to the design of efficient ligands for Gal-4 with diagnostic or therapeutic potential.
Article
Chemistry, Medicinal
Khuchtumur Bum-Erdene, Patrick M. Collins, Matthew W. Hugo, Somayeh S. Tarighat, Fei Fei, Chandan Kishor, Hakon Leffler, Ulf J. Nilsson, John Groffen, I. Darren Grice, Nora Heisterkamp, Helen Blanchard
Summary: In this study, novel Galectin-3 antagonists with enhanced affinity and selectivity were designed and synthesized. Experimental results demonstrated the specific inhibition of Galectin-3-induced BCP-ALL cell agglutination and decreased viability of ALL cells. Therefore, these compounds show promise as important leads for cancer therapeutics.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yi Zheng, Jiyong Su, Michelle C. Miller, Jie Geng, Xuejiao Xu, Tao Zhang, Maksim Mayzel, Yifa Zhou, Kevin H. Mayo, Guihua Tai
Summary: This study reveals a novel interaction mode of galectin-3 with negatively charged sugar molecules and provides structural details. The new sugar orientation and interactions have essentially the same thermodynamic binding parameters as lactose.
Article
Biochemistry & Molecular Biology
Andressa Rocha de Oliveira Sousa, Francisco Regivanio Nascimento Andrade, Renata Pinheiro Chaves, Bruno Lopes de Sousa, Dimas Batista de Lima, Racquel Oliveira da Silva Souza, Claudio Gleidiston Lima da Silva, Claudener Souza Teixeira, Alexandre Holanda Sampaio, Celso Shiniti Nagano, Romulo Farias Carneiro
Summary: CCL, a sponge lectin, with determined primary structure, shows high biotechnological potential and promising results against Leishmania infantum, indicating its potential use in the field of human health.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Chemistry, Organic
Ajaz Ahmed, Debaraj Mukherjee
Summary: This article presents a method for the stereoselective synthesis of orthogonally protected N-substituted beta-aminooxysugars from sugar epoxides and nitrones derived from aromatic aldehydes. Both ether- and ester-protected sugar epoxides can be used in the synthesis. The synthesized aminooxy sugars can react with aldehydes bearing or free reducing sugars under heating conditions to yield N-O-linked 1,1-/1,5/1,6-disaccharide mimetics in good yield.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xiaocong Wang, Melinda S. Hanes, Richard D. Cummings, Robert J. Woods
Summary: Sulfated glycans have significant potential in molecular pathology as biomarkers for various diseases. However, there is a lack of sulfate-recognizing lectins. In this study, a mutated form of E-selectin was engineered using computational approaches, which showed highly specific recognition of 6'-sulfo-sLex. The mutations in the recombinant lectin eliminated repulsions with the sulfate moiety and abolished interactions with the endogenous ligand.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Lingxin Zhu, Yi Tang, Xiao-Yan Li, Samuel A. Kerk, Costas A. Lyssiotis, Xiaoyue Sun, Zijun Wang, Jung-Sun Cho, Jun Ma, Stephen J. Weiss
Summary: The interaction between metalloproteins Mmp9 and Mmp14 and carbohydrate-binding protein galectin-3 plays a critical role in osteoclast-mediated bone resorption. The ability of Mmp9 and Mmp14 to degrade galectin-3 on the cell surface depends on its binding protein Lrp-1. These findings reveal a previously unrecognized galectin-3/Lrp1 axis that regulates both the transcriptional programs and intracellular signaling cascades essential for osteoclast function.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Asumi Uemura, Satomi Nadanaka, Hiroshi Kitagawa, Jun-ichi Tamura
Summary: Researchers synthesized biotinylated chondroitin sulfate tetrasaccharides, CS-AD and CS-DA, in a stereo-controlled manner. It was found that the CS-D-specific monoclonal antibody MO-225 exhibited stronger binding to CS-DA compared to CS-DD or-AD.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Cynthia Buelck, Elisabeth E. L. Nystroem, Tomas Koudelka, Michael Mannbar-Frahm, Gerrit Andresen, Mariem Radhouani, Florian Tran, Franka Scharfenberg, Friederike Schrell, Fred Armbrust, Eileen Dahlke, Bei Zhao, Alex Vervaeke, Franziska Theilig, Philip Rosenstiel, Philipp Starkl, Stephan P. Rosshart, Helmut Fickenscher, Andreas Tholey, Gunnar C. Hansson, Christoph Becker-Pauly
Summary: The metalloproteases meprin alpha and meprin beta are highly expressed in the healthy gut but significantly decreased in inflammatory bowel disease. They form covalently linked heterodimers in the colon, and the major intestinal substrate for these heterodimers is galectin-3. The cleavage of galectin-3 by meprin alpha/beta heterodimers may play a key role in colon host-microbiome homeostasis.
Article
Biochemistry & Molecular Biology
Mohamed Gatie, Danielle M. Spice, Amritpal Garha, Adam McTague, Mariam Ahmer, Alexander Timoshenko, Gregory M. Kelly
Summary: The regulation of protein O-GlcNAcylation plays a crucial role in stem cell pluripotency and differentiation. This study investigates the impact of O-GlcNAc modifications on extraembryonic endoderm (XEN) differentiation, focusing on the protein galectin-3. The results reveal that galectin-3 expression and secretion are influenced by O-GlcNAc modifications, but inhibiting global O-GlcNAcylation does not affect pluripotency or XEN differentiation in embryonic stem (ES) cells.
Article
Biochemistry & Molecular Biology
Stellee M. P. Biscaia, Cassiano Pires, Francislaine A. R. Livero, Daniel L. Bellan, Israel Bini, Silvina O. Bustos, Renata O. Vasconcelos, Alexandra Acco, Marcello Iacomini, Elaine R. Carbonero, Martin K. Amstalden, Fabio R. Kubata, Richard D. Cummings, Marcelo Dias-Baruffi, Fernanda F. Simas, Carolina C. Oliveira, Rilton A. Freitas, Celia Regina Cavichiolo Franco, Roger Chammas, Edvaldo S. Trindade
Summary: Melanoma is a highly metastatic and rapidly progressing cancer. The melanoma microenvironment plays a crucial role in drug resistance and tumor recurrence. The study found that MG-Pe can bind to Galectin-3 and has antitumor and antimetastatic effects, especially when used in combination with chemotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Nadezhda Antipova, Polina Obukhova, Tatiana Ovchinnikova, Oxana Galanina, Ola Blixt, Horst Kunz, Alexander Filatov, Yuriy Knirel, Jacques LePendu, Sergey Khaidukov, Nicolai Bovin
MOLECULAR IMMUNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Anton Zalygin, Daria Solovyeva, Ivan Vaskan, Stephen Henry, Marcel Schaefer, Pavel Volynsky, Alexander Tuzikov, Elena Korchagina, Ivan Ryzhov, Alexey Nizovtsev, Konstantin Mochalov, Roman Efremov, Eleonora Shtykova, Vladimir Oleinikov, Nicolai Bovin
Correction
Cell Biology
Gabriel Garcia Caballero, Donella Beckwith, Nadezhda V. Shilova, Adele Gabba, Tanja J. Kutzner, Anna-Kristin Ludwig, Joachim C. Manning, Herbert Kaltner, Fred Sinowatz, Mare Cudic, Nicolai V. Bovin, Paul V. Murphy, Hans-Joachim Gabius
HISTOCHEMISTRY AND CELL BIOLOGY
(2020)
Article
Cell Biology
Gabriel Garcia Caballero, Donella Beckwith, Nadezhda Shilova, Adele Gabba, Tanja J. Kutzner, Anna-Kristin Ludwig, Joachim C. Manning, Herbert Kaltner, Fred Sinowatz, Mare Cudic, Nicolai Bovin, Paul Murphy, Hans-Joachim Gabius
HISTOCHEMISTRY AND CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Nadezhda Antipova, Polina Obukhova, Oxana Galanina, Mikhail Gorbach, Inna Popova, Sergey Khaidukov, Natalia Grishchenko, Nikolai Tupitsyn, Jacques Le Pendu, Nicolai Bovin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Sergio A. Machado, Momal Sharif, Govindasamy Kadirvel, Nicolai Bovin, David J. Miller
Review
Biochemistry & Molecular Biology
Alexander Tuzikov, Alexander Chinarev, Nadezhda Shilova, Elena Gordeeva, Oxana Galanina, Tatyana Ovchinnikova, Marcel Schaefer, Nicolai Bovin
Summary: Polyacrylamide conjugates of glycans are widely used in glycobiology for immobilizing glycans and as multivalent inhibitors. The biotin tag allows quantitative immobilization on any surface and acts as a tracer for detecting carbohydrate-binding proteins. Additionally, this review covers less common but significant applications, as well as details on the glycopolymers themselves and their interactions with proteins.
GLYCOCONJUGATE JOURNAL
(2021)
Article
Medicine, Research & Experimental
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Yuriy Knirel, Polina Obukhova, Alexey Nokel, Roman Kunetskiy, Svetlana Tsygankova, Daniel Bello-Gil, Cristina Costa, Rafael Manez, Nicolai Bovin
Summary: The study compared the specificity of alpha-Gal antibodies in mice and humans, finding that the mouse model recognizes a broader range of human blood group B epitopes and glycans without the alpha-galactosyl residue compared to human antibodies, which show a preference for binding bacterial polysaccharides. This suggests caution should be exercised in using the mouse model in xenotransplantation experiments when epitope specificity is critical.
XENOTRANSPLANTATION
(2021)
Article
Hematology
Radhika Nagappan, Willy A. Flegel, Kshitij Srivastava, Eleanor C. Williams, Ivan Ryzhov, Alexander Tuzikov, Oxana Galanina, Nadezhda Shilova, Gennady Sukhikh, Holly Perry, Nicolai V. Bovin, Stephen M. Henry
Summary: This study evaluated the feasibility of using SARS-CoV-2 peptide Kode Technology-modified red cells to develop an assay for detecting SARS-CoV-2 antibodies. The results showed that C19-kodecytes have high specificity and sensitivity on routine serologic platforms, making them suitable for serological testing related to COVID-19.
Article
Microbiology
Adrien Breiman, Nathalie Ruvoen-Clouet, Marie Deleers, Tiffany Beauvais, Nicolas Jouand, Jezabel Rocher, Nicolai Bovin, Nathalie Labarriere, Hanane El Kenz, Jacques Le Pendu
Summary: The study reveals that COVID-19 patients have significantly lower levels of anti-Tn antibodies compared to non-infected individuals, indicating both quantitative and qualitative differences. The anti-Tn antibodies are correlated with the development of specific antiviral response and are also lower in individuals with blood types A and B.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Developmental Biology
Momal Sharif, Karl Kerns, Peter Sutovsky, Nicolai Bovin, David J. Miller
Summary: Studies have shown that progesterone-induced sperm release requires active CatSper channels and proteasomal degradation, indicating that hyperactivation and proteolysis are vital parts of the mechanism for sperm movement.
Article
Chemistry, Organic
Alexey V. Nizovtsev, Nicolai V. Bovin
Summary: A new convenient synthetic route for 2-aminopropane-1,2,3-tricarboxylic acid is described, involving two initial steps conducted in a one-pot procedure. The successful synthesis is achieved through the cyclization of hippuric acid and subsequent hydrolysis with HCl.
SYNTHESIS-STUTTGART
(2021)
Article
Immunology
Marina M. Ziganshina, Nadezhda Shilova, Nailia R. Khasbiullina, Anastasia Terentyeva, Elena L. Dolgopolova, Alexey Yu Nokel, Ekaterina L. Yarotskaya, Roman G. Shmakov, Nicolai Bovin, Gennady T. Sukhikh
Summary: A possible mechanism of immune tolerance in pregnancy is the production of blocking antibodies in the placenta, which protect the fetus from the mother's immune system. The specificity and nature of these antibodies are still unknown. However, the study found that the repertoire of placental antibodies is less variable and qualitatively different from those in the peripheral blood, and this repertoire is narrower in women with pre-eclampsia compared to healthy pregnancy. Some antibodies are found exclusively in the placenta, while others are only found in the placenta of healthy women.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
E. Slivka, A. B. Tuzikov, S. Khaidukov, V. A. Komarova, S. M. Henry, N. Bovin, E. M. Rapoport
Summary: This study compared the properties of three synthetic analogs and found that they have similar localization patterns and can be interchanged when studying the incorporation/release processes. However, there are some differences in the insertion process and release kinetics.
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
(2022)
Review
Nanoscience & Nanotechnology
A. Yu Nokel, N. Bovin, N. Shilova
Summary: The review discusses the main methods for modifying plastic as a functional material for manufacturing biochips, including physical, chemical, and physical-chemical methods for introducing functional groups and achieving effective immobilization of biomolecules. The advantages and disadvantages of existing approaches are discussed in terms of their applicability to various tasks.
NANOBIOTECHNOLOGY REPORTS
(2022)