Article
Oncology
Borwyn A. Wang, Hrishikesh M. Mehta, Srinivasa R. Penumutchu, Blanton S. Tolbert, Chonghui Cheng, Marek Kimmel, Torsten Haferlach, Jaroslaw P. Maciejewski, Seth J. Corey
Summary: This study discovers two different isoforms of CSF3R in myelodysplastic syndromes and identifies the role of the splicing factor SRSF2 in regulating their expression, which affects granulopoiesis.
Article
Biochemistry & Molecular Biology
Ali Calderon-Aparicio, Bi-Dar Wang
Summary: Prostate cancer is the most frequently diagnosed cancer in American men and often leads to treatment resistance, relapse, and decreased survival rates. Aberrant RNA splicing, which increases proteome diversity, has been linked to prostate cancer development and progression. Targeting aberrant splicing mechanisms through pharmacological and molecular approaches offers potential for improved clinical outcomes in prostate cancer patients.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Oncology
Xiangyu Li, Woonghee Kim, Muhammad Arif, Chunxia Gao, Andreas Hober, David Kotol, Linnea Strandberg, Bjorn Forsstrom, Asa Sivertsson, Per Oksvold, Hasan Turkez, Morten Grotli, Yusuke Sato, Haruki Kume, Seishi Ogawa, Jan Boren, Jens Nielsen, Mathias Uhlen, Cheng Zhang, Adil Mardinoglu
Summary: Pyruvate kinase muscle type (PKM) is a key enzyme in glycolysis and plays an important role in cancer. The association between PKM expression and the survival outcome of patients with different cancers is controversial. System biology methods were employed to reveal the prognostic value and potential biological functions of PKM transcripts in different human cancers.
Article
Cell Biology
Erliang Guo, Xionghui Mao, Xueying Wang, Lunhua Guo, Changming An, Cong Zhang, Kaibin Song, Guohui Wang, Chunbin Duan, Xiwei Zhang, Xianguang Yang, Zhennan Yuan, Ji Sun, Xiaomei Li, Weiwei Yang, Hongxue Meng, Susheng Miao
Summary: High expression of ANLN is associated with poor prognosis in HNSCC patients. The two major splice variants of ANLN, ANLN-201 and ANLN-210, are highly expressed in HNSCC tissues and cell lines. ANLN promotes tumor growth and invasion in HNSCC by interacting with c-Myc and hnRNPC in different mechanisms.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Seungwoo Cha, Chang Pyo Hong, Hyun Ah Kang, Ji-Sook Hahn
Summary: Gcr1, an important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently found to have two isoforms, Gcr1(U) and Gcr1(S), produced from un-spliced and spliced transcripts. Gcr1(U) forms an active complex with Gcr2 homodimer, whereas Gcr1(S) acts as a homodimer without Gcr2.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sana Fatima, Swati Gupta, Abdul Burhan Khan, Sayeed Ur Rehman, Mohamad Aman Jairajpuri
Summary: Alpha-1-antichymotrypsin (ACT) is a protein that regulates the activity of proteases and is associated with diseases. Through bioinformatics and molecular biology, a novel isoform of ACT with a new N-terminus has been discovered, which lacks a signal sequence but retains activity. Additionally, a truncated transcript lacking certain structural elements has been identified, suggesting a potential non-inhibitory role. Further investigation is required to validate these findings.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lennart Saenger, Julian Bender, Katja Rostowski, Ralph Golbik, Hauke Lilie, Carla Schmidt, Sven-Erik Behrens, Susann Friedrich
Summary: Our study reveals that AUF1 can directly promote the binding of miRNA to its target site, thus modulating gene expression; the RNA remodelling activities of AUF1 are isoform-specific, with isoforms containing the YGG motif acting as competent RNA chaperones.
Article
Biochemistry & Molecular Biology
Monikaben Padariya, Robin Fahraeus, Ted Hupp, Umesh Kalathiya
Summary: The study reveals the binding specificity of UPF1 with different mRNA motifs and the impact of insertion on UPF1 catalytic activity. Experimental results demonstrate two structural conformations of UPF1 and enhance understanding of the NMD pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Sophie K. F. De Salis, Lanxin Li, Zheng Chen, Kam Wa Lam, Kristen K. Skarratt, Thomas Balle, Stephen J. Fuller
Summary: This review summarizes recent advances in understanding the structure and function of alternative spliced (AS) P2X7 isoforms, as well as their associations with cancer and potential role in modulating the inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Jiaqian Liang, Ze Hong, Boyue Sun, Zhaoxi Guo, Chen Wang, Juanjuan Zhu
Summary: Alternative splicing of pre-mRNA generates protein isoforms that increase diversity, affecting biological processes like innate immunity. The cGAS-STING pathway, with key molecules undergoing alternative splicing, regulates innate immunity. Protein isoforms in this pathway exhibit different functions in interferon response and can help host avoid pathway overactivation. Viruses can also use alternative splicing to resist host antiviral responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ashley Lui, Robert Sparks, Rekha Patel, Niketa A. Patel
Summary: This study identified a novel sortilin splice variant related to glucose metabolism, whose presence may be associated with insulin resistance in adipocytes. This finding provides a new potential target for the management of type 2 diabetes and metabolic syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Saleh Althenayyan, Mohammed H. H. AlMuhanna, Abdulkareem AlAbdulrahman, Bandar Alghanem, Suliman A. A. Alsagaby, Abdulaziz Alfahed, Glowi Alasiri, Mohammad Azhar Aziz
Summary: There is a need for prognostic biomarkers in colorectal cancer metastasis for personalized medicine. Alternative splicing analysis identified ADAM12 and MUC4 as strong biomarker candidates. Their isoform expression was characterized in colorectal cancer cell lines and validated in patient cohorts. The study also demonstrated the potential of isoforms as predictive biomarkers in response to treatment. This comprehensive study highlights the importance of studying alternative splicing isoforms for cancer prognosis.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Genetics & Heredity
Achala Fernando, Chamikara Liyanage, Afshin Moradi, Panchadsaram Janaththani, Jyotsna Batra
Summary: This study comprehensively characterized IRX4 in prostate cancer, identifying novel IRX4 transcripts and protein isoforms, shedding light on its potential role in PCa progression.
Article
Cell Biology
Claire Bryant, Amy Webb, Alexander S. Banks, Dawn Chandler, Rajgopal Govindarajan, Shipra Agrawal
Summary: Podocytes are specialized epithelial cells that play a crucial role in maintaining kidney function. They express specific variants of the PPAR gamma gene, which are different from those found in adipose tissue. Understanding these podocyte-specific PPAR gamma variants could lead to the development of targeted therapeutic treatments.
Article
Immunology
QinLei Gu, Kenneth S. Tung, Ulrike M. Lorenz
Summary: To achieve a healthy and functional immune system, there is a delicate balance between the activation of Tcon cells and the suppression by Treg cells. SHP-1 plays a role in modulating this balance by regulating Tcon cell resistance to Treg-mediated suppression. However, the role of SHP-1 in Treg function is still not fully understood.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zutan Li, Bingbing Jin, Jingya Fang
Summary: In this study, we propose MetaAc4C, an advanced deep learning model for accurate identification of N4-acetylcytidine (ac4C) sites using pre-trained BERT and various optimization techniques. By adapting generative adversarial networks to address data imbalance and augmenting training RNA samples, our model outperforms existing methods in terms of ACC, MCC, and AUROC.