Journal
GENOMICS
Volume 100, Issue 4, Pages 252-263Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2012.06.012
Keywords
Abiotic stress; Protoplast co-transfection; Dehydration-responsive element (DRE); Protein-protein docking; Setaria italica; WD40 protein; Electrophoretic mobility shift assay (EMSA)
Funding
- NIPGR
- Council of Scientific and Industrial Research
- NIPGR, New Delhi
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A differentially expressed transcript, encoding a putative WD protein (Setaria italica WD40:SiWD40), was identified in foxtail millet. Tertiary structure modeling revealed that its C-terminus possesses eight blade beta-propeller architecture. Its N-terminal has three alpha-helices and two 3(10)-helices and was highly induced by different abiotic stresses. The SiWD40:GFP fusion protein was nuclear localized. Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (ORE). A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter. Thus, for the first time, we report that DREs probably regulate expression of SiWD40 during environmental stress. Molecular docking analysis revealed that the circumference of the beta-propeller structure was involved in an interaction with a SiCullin4 protein, supporting the adaptability of SiWD40 to act as a scaffold. Our study thus provides a vital clue for near future research on the stress-regulation of WD proteins. (C) 2012 Elsevier Inc. All rights reserved.
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