Article
Microbiology
Qiushui Wang, Xin Zhou, Yue Liu, Yan Han, Jia Zuo, Jie Deng, Liyan Yuan, Lijuan Gao, Wenbo Bai
Summary: This study showed that the application of mixed oligosaccharides had significant effects on the bacterial communities in cucumber plants' phyllosphere, rhizosphere, and bulk soil. The presence of mixed oligosaccharides increased the abundance of potential growth-promoting bacteria and altered bacterial interaction networks, leading to improved plant growth and yield.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Tao Wang, Xiaohong Lu, Chenggang Xiang, Xiaojun Li, Wenqian Liu, Qing Wang, Zixi Liu, Lihong Gao, Wenna Zhang
Summary: This study identified 221 CsaP450 genes in the cucumber genome and found that 15 of them can be systemically transported in graft experiments, showing specific enrichment in oxidoreductase activity and secondary metabolic pathways. Comparison with other plants revealed differences in the structures and motifs of these cucumber CsaP450 genes.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Biochemical Research Methods
Hironobu Fujita, Ayane Osaku, Yuto Sakane, Koki Yoshida, Kayoko Yamada, Seia Nara, Takahito Mukai, Masayuki Su'etsugu
Summary: In this study, the negative supercoiling of whole genomes extracted from Escherichia coli and Vibrio natriegens cells was achieved using a nick/gap repair and negative supercoiling reaction. Supercoiled chromosomes were separated by size and enzymatic replication of chromosomes was also achieved. The development of genetically stable chromosomes allowed for successful electroporation and will greatly accelerate the democratization of genome synthetic biology.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yunyan Kang, Jiale Tong, Wei Liu, Zhongli Jiang, Gengzheng Pan, Xianpeng Ning, Xian Yang, Min Zhong
Summary: In this study, 37 putative cucumber MLP genes were identified and classified into three groups. It was found that only tandem duplication occurred in the evolution of these genes. The promoter region of these CsMLP genes contained multiple regulatory cis-elements related to stress, hormone, light, and growth response, suggesting their involvement in various biological processes. Transcriptome analysis revealed a reprogramming of MLPs expression in response to Phytophthora melonis infection. Knockdown and overexpression experiments demonstrated the involvement of CsMLP1 and CsMLP5 in disease tolerance and lesion expansion. Overall, this study provides insights into the function and evolutionary relationship of MLP genes in cucumber.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Hosakatte Niranjana Murthy, Yaser Hassan Dewir, Dayanand Dalawai, Nasser Al-Suhaibani
Summary: This study assessed the chemical composition of wild cucumber seeds and seed oils, finding that wild cucumber seed oil is rich in fatty acids, proteins, carbohydrates, minerals, and fiber, making it a potential raw material in food applications.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Vera S. Bogdanova, Natalia V. Shatskaya, Anatoliy V. Mglinets, Oleg E. Kosterin, Gennadiy V. Vasiliev
Summary: Plastids and mitochondria have their own unique genomes and evolution paths, with six different structural types of the pea mitochondrial genome identified in this study. The discordant inheritance of plastids and mitochondria in wild peas may be due to plastid-nuclear incompatibility, impacting organelle inheritance. The analysis of a nuclear gene, His5, indicated that wild peas in Southern Europe likely originated from hybridization between peas from the eastern Mediterranean region.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2021)
Article
Environmental Sciences
Xudong Ma, Yanfang Gu, Chanjuan Liang
Summary: This study found that rice showed higher tolerance to MCs stress compared to cucumber, possibly by regulating PPs activity. Among PPs, PP2A activity was more sensitive to MCs, and rice had higher expression levels of PP2A and less decrease in PP2A activity compared to cucumber leaves.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Ian Small, Joanna Melonek, Alexandra-Viola Bohne, Joerg Nickelsen, Christian Schmitz-Linneweber
Summary: Plant organellar RNA metabolism is regulated by numerous nucleus-encoded RNA-binding proteins (RBPs) that control RNA stability, processing, and degradation. These post-transcriptional processes are critical for the production of essential components in chloroplasts and mitochondria, which are necessary for organellar biogenesis and plant survival. While many organellar RBPs have been identified, our understanding of their mechanisms and kinetics in fulfilling their functions remains incomplete. This review provides a comprehensive summary of plant organellar RNA metabolism, focusing on the mechanistic aspects and kinetics of RBP functions.
Article
Biochemistry & Molecular Biology
Zhixing Qing, Yao Shi, Lida Han, Pengkun Li, Ziou Zha, Chang Liu, Xiubin Liu, Peng Huang, Yisong Liu, Qi Tang, Kewu Zeng, Jianguo Zeng, Yuan Zhou
Summary: This study isolated ten cucurbitacin C analogues, including six compounds with inhibitory effects on tumor cell lines. Cucurbitacin C6 and C7 exhibited significant inhibition on HepG2 cell line. Cucumber is an important source of specialized metabolites in the food and medicinal industries.
Review
Plant Sciences
Rebecca Grumet, Ying-Chen Lin, Stephanie Rett-Cadman, Ajaz Malik
Summary: Cucumber fruits exhibit extensive variation in morphological features such as size, shape, waxiness, spines, warts, and flesh thickness. Genetic factors influencing floral organ establishment, cell division and cell cycle regulation, hormone biosynthesis and response, sugar transport, trichome development, and cutin, wax, and pigment biosynthesis have been identified as factors influencing cucumber fruit morphology. Understanding and manipulating these genetic factors can lead to the development of cucumber varieties with desired traits and improved qualities for consumers.
Article
Biochemistry & Molecular Biology
Lin Chen, Fenghua Zheng, Zili Feng, Yue Li, Muxuan Ma, Guoping Wang, Hongbo Zhao
Summary: The research found that the expression of the CsVI2 gene and the activity of vacuolar invertase in cucumber seedlings were significantly induced by drought stress. In addition, drought stress led to changes in the expression of certain related genes and alterations in the activities of sucrose metabolism enzymes. Transgenic cucumber seedlings overexpressing CsVI2 exhibited enhanced vacuolar invertase activity and drought tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Shuangshuang Zhang, Yanshuo Liang, Jie Zhang, Stefano G. A. Draisma, Delin Duan
Summary: The study compared the mitochondrial and chloroplast genomes of Sargassum polycystum and Sargassum plagiophyllum, finding shared conserved synteny, sequence regions, and gene number among these and other Sargassum species. The synonymous and non-synonymous substitution rates in the mitochondrial and chloroplast genomes showed significant differences, highlighting divergence between the two types of organelle genomes. Phylogenetic analysis confirmed the close relationship between the two sequenced species and S. ilicifolium.
Article
Plant Sciences
Omar Aristeo Pena-Moran, Jesus Jimenez-Perez, Litzia Ceron-Romero, Maribel Rodriguez-Aguilar
Summary: This study examines the toxic activity of colchicine on cucumber plants and finds a high degree of similarity between cucumber and human tubulins, suggesting potential ecotoxicological concerns when using microtubule inhibitors.
Article
Biology
Tomas Panek, Dovile Barcyte, Sebastian C. Treitli, Kristina Zahonova, Martin Sokol, Tereza Sevcikova, Eliska Zadrobilkova, Karin Jaske, Naoji Yubuki, Ivan Cepicka, Marek Elias
Summary: By studying the newly discovered non-photosynthetic lineage Leontynka, new insights into organellar genome evolution in Chlamydomonadales can be gained. Leontynka’s unique characteristics of plastid and mitochondrial genomes make it an interesting model for studying organellar genome evolution.
Article
Plant Sciences
Zelong Zhang, Haifang He, Minghui Yan, Chenchen Zhao, Caiyan Lei, Jingjing Li, Fengming Yan
Summary: The study revealed that CCYV infection altered plant metabolism by suppressing defensive flavonoids and terpenoids metabolism while triggering lipids, amino acids, and nucleotides metabolism. This makes plants more susceptible to whitefly attack, ultimately facilitating virus transmission.
Article
Chemistry, Physical
Sabine Holz, Michael Kube, Grzegorz Bartoszewski, Bruno Huettel, Carmen Buettner
Article
Agronomy
Karolina Kazminska, Ewelina Hallmann, Anna Rusaczonek, Aleksandra Korzeniewska, Miroslaw Sobczak, Joanna Filipczak, Karol Seweryn Kuczerski, Jaroslaw Steciuk, Monika Sitarek-Andrzejczyk, Marek Gajewski, Katarzyna Niemirowicz-Szczytt, Grzegorz Bartoszewski
MOLECULAR BREEDING
(2018)
Article
Agronomy
Renata Slomnicka, Helena Olczak-Woltman, Aleksandra Korzeniewska, Dariusz Gozdowski, Katarzyna Niemirowicz-Szczytt, Grzegorz Bartoszewski
MOLECULAR BREEDING
(2018)
Article
Biochemistry & Molecular Biology
Barczak-Brzyzek Anna, Brzyzek Grzegorz, Koter Marek, Gawronski Piotr, Filipecki Marcin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Plant Sciences
Piotr Gawronski, Aleksandra Palac, Lars B. Scharff
Article
Genetics & Heredity
Karolina Kazminska, Ewelina Hallmann, Aleksandra Korzeniewska, Katarzyna Niemirowicz-Szczytt, Grzegorz Bartoszewski
Article
Agronomy
Malgorzata Tanska, Dorota Ogrodowska, Grzegorz Bartoszewski, Aleksandra Korzeniewska, Iwona Konopka
Review
Biochemistry & Molecular Biology
Jakub Mielecki, Piotr Gawronski, Stanislaw Karpinski
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biotechnology & Applied Microbiology
Anna Hawliczek, Leszek Bolibok, Katarzyna Tofil, Ewa Borzecka, Joanna Jankowicz-Cieslak, Piotr Gawronski, Adam Kral, Bradley J. Till, Hanna Bolibok-Bragoszewska
Article
Plant Sciences
Piotr Gawronski, Pawel Burdiak, Lars B. Scharff, Jakub Mielecki, Magdalena Gorecka, Magdalena Zaborowska, Dario Leister, Cezary Waszczak, Stanislaw Karpinski
Summary: Chloroplast-to-nucleus retrograde signaling is crucial for cell function, plant growth, and development. The discovery of dual-localized transcription factors sheds light on the communication between chloroplasts and the nucleus, contributing to a better understanding of the signaling events in the chloroplast-nucleus cross-talk.
Article
Cell Biology
Piotr Gawronski, Christel Enroth, Peter Kindgren, Sebastian Marquardt, Stanislaw Karpinski, Dario Leister, Poul Erik Jensen, Jeppe Vinther, Lars B. Scharff
Summary: The study revealed that mRNA secondary structure influences translation in plastids, with increased accessibility of the translation initiation region of psbA mRNA after exposure to high light. The presence of a putative regulatory protein in the 5 ' UTR of psbA was identified, which may cause structural opening of the translation initiation region, facilitating ribosome access. This suggests that changes in mRNA secondary structure could represent a general mechanism for translational regulation of psbA and other plastid genes.
Article
Biochemistry & Molecular Biology
Renata Slomnicka, Helena Olczak-Woltman, Miroslaw Sobczak, Grzegorz Bartoszewski
Summary: This study characterized the cytological and transcriptomic response of cucumber to Pseudomonas syringae pv. lachrymans, finding that different cucumber lines exhibited distinct responses to the pathogen, with the resistant line Gy14 showing a more massive transcriptomic response compared to the susceptible line B10. This suggests that dynamic transcriptomic response may be related to host resistance against the invading pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Denise F. Dostatny, Aleksandra Korzeniewska, Grzegorz Bartoszewski, Ryszard Rawski, Karolina Kazminska, Bronislovas Gelvonauskis
Summary: This study compiles the results of three-year expeditions aimed at plant crop collection in Lithuania, Eastern Europe. A total of 1010 samples of seeds, bulbs, and plant grafts were obtained, with majority belonging to families like Cucurbitaceae, Fabaceae, and Solanaceae. The collected material could be valuable for breeding programs and genetic diversity studies.
Review
Biotechnology & Applied Microbiology
Emilia Olechowska, Renata Slomnicka, Karolina Kazminska, Helena Olczak-Woltman, Grzegorz Bartoszewski
Summary: Cold stress is a major cause of yield losses in plants in temperate climates. Improving cold tolerance in cucumber, which is sensitive to low temperatures, would benefit cucumber producers. Despite numerous studies, the molecular mechanisms of cold tolerance in cucumber have not been fully elucidated.
JOURNAL OF APPLIED GENETICS
(2022)
Article
Cell Biology
Pawel Burdiak, Jakub Mielecki, Piotr Gawronski, Stanislaw Karpinski
Summary: In Arabidopsis, CRK5 acts as a negative regulator of leaf aging and is antagonistically regulated by WRKY53. They play roles in chlorophyll synthesis/degradation, senescence, and stomatal conductance.