Article
Virology
Daniel D. T. Andrews, Marli Vlok, Dorssa Akbari Bani, Brenna N. Hay, Yasir Mohamud, Leonard J. Foster, Honglin Luo, Christopher M. Overall, Eric Jan
Summary: Host antiviral factors use the retinoic acid-inducible gene I (RIG-I)-like receptor pathway to sense virus infection and trigger the production of interferons, which establish an antiviral state. The chaperone protein 14-3-3 & epsilon; plays a key role in this pathway by facilitating the delivery of the viral sensor protein RIG-I to the mitochondria. However, the enteroviral 3C protease cleaves 14-3-3 & epsilon; during infection, disrupting its function and promoting virus infection. This study reveals a novel viral strategy that evades the host antiviral response.
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Jun Kang, Mengqian Huang, Jinyu Li, Keke Zhang, Cheng Zhu, Sihua Liu, Zhenwei Zhou, Tao Wang, Zhiyun Wang
Summary: EV-D68 is a globally emerging pathogen causing severe respiratory illnesses mainly in children. The structural protein VP3 of EV-D68 interacts with IRF7 and inhibits its phosphorylation and nuclear translocation, resulting in the repression of IFN production. VP3 also inhibits the ubiquitination of IRF7 induced by TRAF6 through competitive interaction. The control of IRF7 by VP3 may contribute to the subversion of host innate immune responses by EV-D68.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Megan Culler Freeman, Alexandra Wells, Jessica Ciomperlik-Patton, Michael M. Myerburg, Liheng Yang, Jennifer Konopka-Anstadt, Carolyn B. Coyne
Summary: EV-D68 virus is associated with severe respiratory illness and acute flaccid myelitis, potentially transmitted through respiratory and enteric routes. Recent isolates show decreased sensitivity to acid and temperature, with respiratory epithelium inducing a robust defense response to restrict infection.
Article
Chemistry, Medicinal
Linjie Yan, Ruiyuan Cao, Hongjie Zhang, Yuexiang Li, Wei Li, Xiaoyuan Li, Shiyong Fan, Song Li, Wu Zhong
Summary: Phosphoamidate derivatives of nucleoside NITD008 were synthesized and evaluated for their antiviral activities against EV71 and EV-D68, with compounds 15f and 151 showing the most promising results. Compound 151 demonstrated the highest selectivity index against both viruses, indicating its potential as a candidate for antiviral drug development in treating EV71 and EV-D68 infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Hui Li, Yunfang Yao, Yu Chen, Shuangling Zhang, Zhi Deng, Wentao Qiao, Juan Tan
Summary: In this study, the interaction between immune-associated protein TRAF3 interacting protein 3 (TRAF3IP3) and EV71 3C(pro) was investigated. It was found that TRAF3IP3 can inhibit EV71 replication, while 3C(pro) can resist this inhibition through proteolytic cleavage of TRAF3IP3. The nuclear export signal (NES) of TRAF3IP3 plays a role in altering the localization of 3C(pro) and inhibiting EV71 replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Yuling Zhang, Leling Xu, Zhe Zhang, Xin Su, Zhiyun Wang, Tao Wang
Summary: This study reveals a novel mechanism by which EV-D68 inhibits ISG transcription and antagonizes the antiviral responses of host type I interferon. SOCS3 plays a key role in this process by inhibiting the phosphorylation of STAT3. A designed SOCS3 inhibitor can significantly reduce the levels of EV-D68.
Article
Virology
Xiaoman Wo, Yuan Yuan, Yong Xu, Yang Chen, Yao Wang, Shuoxuan Zhao, Lexun Lin, Xiaoyan Zhong, Yan Wang, Zhaohua Zhong, Wenran Zhao
Summary: This study revealed that during EV-A71 infection, TDP-43 undergoes cleavage and translocation to the cytoplasm, mediated by viral proteases. This suggests a potential role of TDP-43 in the pathogenesis of EV-A71 infection.
Review
Immunology
Furong Qing, Zhiping Liu
Summary: Interferon regulatory factor 7 (IRF7) is a crucial member of the IRFs family, located downstream of the pattern recognition receptors (PRRs)-mediated signaling pathway, and plays a vital role in the production of type I interferon (IFN-I). Its activation can inhibit various viral and bacterial infections, suppress the growth and metastasis of certain cancers, but it may also impact the tumor microenvironment and promote the development of other cancers.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Yan Deng, Sheng-lan Guo, Jia-quan Li, Shan-shan Xie, Ying-chuan Zhou, Bin Wei, Qian Wang, Fen Wang
Summary: IRF7 upregulation attenuates pulmonary vascular remodeling, decreases pulmonary arterial pressure, improves right ventricular structure and function, and may serve as a potential molecular target for PH therapy.
Article
Microbiology
Rui Liu, Li Gao, Fuchun Yang, Xiaohan Li, Changjun Liu, Xiaole Qi, Hongyu Cui, Yanping Zhang, Suyan Wang, Xiaomei Wang, Yulong Gao, Kai Li
Summary: The study reveals that duck enteritis virus (DEV) evades host immune response by inhibiting the DNA sensing pathway through targeting interferon regulatory factor 7 (IRF7). This finding provides new insights into DNA sensing in ducks and the mechanisms of DEV to evade host antiviral immunity.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Chahui Tan, Xingliang Qin, Yongyao Tan, Xinhuai Dong, Delin Chen, Linyue Liang, Jinling Li, Ruoxi Niu, Kaiyuan Cao, Zhenjian He, Guohong Wei, Mingxing Huang, Xun Zhu
Summary: Enterovirus A71 (EV-A71) is a major global health threat and it has been found that the host restriction factor SHFL can inhibit EV-A71 replication through interacting with its 3Dpol protein. This study validated the upregulation of SHFL in response to EV-A71 infection and identified the molecular mechanism by which SHFL promotes the degradation of EV-A71 3Dpol via the ubiquitin-proteasome pathway. These findings greatly enhance our understanding of the interaction between host and EV-A71.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Junzhuo Si, Xia Tang, Lei Xu, Huichao Fu, Huayi Li, Yonglin He, Jiajia Bao, Jialing Tang, Anlong Li, Nan Lu, Chun Yang
Summary: This study identified significantly altered circRNAs, lncRNAs, miRNAs, and mRNAs pathways in RD cells infected with EV-D68, building a ceRNA regulatory network centered on miRNA. The majority of differentially expressed mRNAs were found to be related to DNA binding, transcription regulation, and various signaling pathways. The hub mRNAs including EGR1, Fos, and Jun were screened out through a protein interaction network. The Fos and ARRDC3 genes were demonstrated to inhibit EV-D68 viral replication in RD cells, suggesting potential drug targets for further research.
Article
Virology
Huan Zhang, Pengfei Jiang, Zeliang Chen, Dang Wang, Yanrong Zhou, Xinyu Zhu, Shaobo Xiao, Liurong Fang
Summary: An epidemic of Norovirus has been occurring worldwide recently. This study found that NoV3CL(pro) acts as a new interferon antagonist by cleaving NEMO, suppressing interferon production.
Article
Virology
Shanshan Fan, Zihang Xu, Pengfei Liu, Yali Qin, Mingzhou Chen
Summary: Several viruses inhibit the formation of RNA processing bodies, including enterovirus 71, which relies on its 2A protease to block P-body formation and promote viral replication. The interaction between DDX6 and viral RNA also facilitates viral replication.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Tran Thao Vy Le, Phuc-Chau Do
Summary: Hand, foot, and mouth disease is a common infection with no available vaccination or therapy. Current research focuses on the differences between strains of Enterovirus-A71, requiring testing of antiviral agents on multiple strains or mutations. This study evaluated previously reported inhibitors and investigated potential candidates using molecular docking, discovering that Hesperidin might be the most promising candidate for inhibiting various strains of EV-A71.
FRONTIERS IN MICROBIOLOGY
(2022)
Editorial Material
Infectious Diseases
Dafna Yahav, Dana Yelin, Isabella Eckerle, Christiane S. Eberhardt, Jianwei Wang, Bin Cao, Laurent Kaiser
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Chaolin Huang, Lixue Huang, Yeming Wang, Xia Li, Lili Ren, Xiaoying Gu, Liang Kang, Li Guo, Min Liu, Xing Zhou, Jianfeng Luo, Zhenghui Huang, Shengjin Tu, Yue Zhao, Li Chen, Decui Xu, Yanping Li, Caihong Li, Lu Peng, Yong Li, Wuxiang Xie, Dan Cui, Lianhan Shang, Guohui Fan, Jiuyang Xu, Geng Wang, Ying Wang, Jingchuan Zhong, Chen Wang, Jianwei Wang, Dingyu Zhang, Bin Cao
Summary: This study aimed to investigate the long-term health consequences of discharged COVID-19 patients and associated risk factors, particularly disease severity. Patients with more severe illness during hospitalization showed more severe impaired pulmonary diffusion capacities and abnormal chest imaging manifestations, highlighting the need for targeted interventions for long-term recovery.
Article
Multidisciplinary Sciences
Pei Li, Ruixuan Guo, Yan Liu, Yingtao Zhang, Jiaxin Hu, Xiuyuan Ou, Dan Mi, Ting Chen, Zhixia Mu, Yelin Han, Zihan Chen, Zhewei Cui, Leiliang Zhang, Xinquan Wang, Zhiqiang Wu, Jianwei Wang, Qi Jin, Zhaohui Qian
Summary: Bat coronavirus RaTG13 shares high genome similarity with SARS-CoV-2 and uses human ACE2 for entry. Various animal ACE2s, including Rhinolophus affinis bat ACE2, can act as entry receptors for SARS-CoV-2 and RaTG13. Mutagenesis analysis identified critical residues in spike proteins for recognition of host ACE2. These findings contribute to understanding coronavirus entry, host range, and virus-host coevolution.
Article
Multidisciplinary Sciences
Yisha Liang, Guigen Zhang, Qiheng Li, Lin Han, Xiaoyou Hu, Yu Guo, Wanyin Tao, Xiaomin Zhao, Mingzhe Guo, Tianyu Gan, Yimin Tong, Yongfen Xu, Zhuo Zhou, Qiang Ding, Wensheng Wei, Jin Zhong
Summary: Through a genome-wide CRISPR-Cas9 screening, the study identified E3 ligase TRIM26 as a critical host factor for HCV replication, with its deficiency impairing HCV genome replication. Mechanistic studies revealed that TRIM26 mediates the ubiquitination of HCV-encoded NS5B protein and promotes HCV genome replication, while the unique structure of mouse TRIM26 prevents its support for HCV replication, unlike human TRIM26 which can promote HCV infection.
Article
Immunology
Wenjing Wang, Zhuo Zhou, Xia Xiao, Zhongqin Tian, Xiaojing Dong, Conghui Wang, Li Li, Lili Ren, Xiaobo Lei, Zichun Xiang, Jianwei Wang
Summary: The study found that SARS-CoV-2 nsp12 suppresses host antiviral responses by attenuating specific IFN promoter activation and impacting the function of IRF3. These findings provide new insights into the viral pathogenesis.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Letter
Medicine, General & Internal
Zhiqiang Wu, Qi Jin, Guizhen Wu, Jian Lu, Mingkun Li, Deyin Guo, Ke Lan, Luzhao Feng, Zhaohui Qian, Lili Ren, Wenjie Tan, Wenbo Xu, Weizhong Yang, Jianwei Wang, Chen Wang
Article
Biology
Shiyou Zhu, Ying Liu, Zhuo Zhou, Zhiying Zhang, Xia Xiao, Zhiheng Liu, Ang Chen, Xiaojing Dong, Feng Tian, Shihua Chen, Yiyuan Xu, Chunhui Wang, Qiheng Li, Xuran Niu, Qian Pan, Shuo Du, Junyu Xiao, Jianwei Wang, Wensheng Wei
Summary: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a global health crisis. Through genome-wide screening, novel host factors (LDLRAD3, TMEM30A, and CLEC4G) have been identified as functional receptors for SARS-CoV-2, playing critical roles in infection.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Changchang Cao, Zhaokui Cai, Xia Xiao, Jian Rao, Juan Chen, Naijing Hu, Minnan Yang, Xiaorui Xing, Yongle Wang, Manman Li, Bing Zhou, Xiangxi Wang, Jianwei Wang, Yuanchao Xue
Summary: Researchers have used vRIC-seq technology to reveal the tertiary structure of the SARS-CoV-2 genome in virions, finding an unentangled globule conformation. They also discovered the role of long-range duplexes and higher-order junctions in the sequential packaging of the viral genome, with some mutations potentially contributing to more stable duplex structures.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Xiaoman Liu, Fengwen Xu, Lili Ren, Fei Zhao, Yu Huang, Liang Wei, Yingying Wang, Conghui Wang, Zhangling Fan, Shan Mei, Jingdong Song, Zhendong Zhao, Shan Cen, Chen Liang, Jianwei Wang, Fei Guo
Summary: MARCH8 acts as an important player in the host anti-influenza virus defense by targeting M2 protein and inhibiting viral replication. The discovery that H1N1 influenza A virus has evolved to resist MARCH8-mediated ubiquitination and degradation sheds light on the inhibitory effects of MARCH8 on virus transmission within the human population.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Jianyuan Zhao, Yongxin Zhang, Minghua Wang, Qian Liu, Xiaobo Lei, Meng Wu, SaiSai Guo, Dongrong Yi, Quanjie Li, Ling Ma, Zhenlong Liu, Fei Guo, Jianwei Wang, Xiaoyu Li, Yucheng Wang, Shan Cen
Summary: Three compounds were identified to exhibit remarkable potency in inhibiting RNA synthesis driven by SARS-CoV-2 RdRp, showing potential for drug development against COVID-19.
ACS INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Chi Zhang, Weiyun Li, Xiaobo Lei, Zhenfei Xie, Linlin Qi, Hui Wang, Xia Xiao, Jun Xiao, Yuxiao Zheng, Chen Dong, Xin Zheng, Shiyang Chen, Jianfeng Chen, Bing Sun, Jun Qin, Qiwei Zhai, Jinsong Li, Bin Wei, Jianwei Wang, Hongyan Wang
Summary: The study reveals that in viral infection, LPA suppresses interferon production through the LPA1 receptor, affecting immune responses. Additionally, LPA1 and ROCK1/2 may impact viral infections by promoting vascular leaking or lung fibrosis. Therefore, targeting the LPA1-ROCK module could be beneficial for treating SARS-CoV-2 or other virus infections.
Article
Cell Biology
Shaojun Zhang, Wenze Huang, Lili Ren, Xiaohui Ju, Mingli Gong, Jian Rao, Lei Sun, Pan Li, Qiang Ding, Jianwei Wang, Qiangfeng Cliff Zhang
Summary: Comparative analysis revealed common and virus-specific host responses, identifying vRNA-associated proteins that either promote or restrict viral infection. The study highlighted how SARS-CoV-2 hijacks the host factor IGF2BP1 to stabilize vRNA and enhance viral translation. Interactome-informed drug repurposing led to the identification of potential broad-spectrum antivirals, including Cepharanthine and Trifluoperazine, with efficacy against the emerging SARS-CoV-2 B.1.351 variant.
Article
Biochemistry & Molecular Biology
Yuehui Zhang, Limin Shang, Jing Zhang, Yuchen Liu, Chaozhi Jin, Yanan Zhao, Xiaobo Lei, Wenjing Wang, Xia Xiao, Xiuyuan Zhang, Yujiao Liu, Linlin Liu, Meng-Wei Zhuang, Qingkun Mi, Chunyan Tian, Jianwei Wang, Fuchu He, Pei-Hui Wang, Jian Wang
Summary: The study systematically investigates the interactions between 29 viral proteins and human cells, revealing key proteins involved in SARS-CoV-2 infection. It identifies the membrane protein ITGB1 as a mediator of viral entry and uncovers proteins that inhibit the interferon pathway. The research proposes potential drugs for COVID-19 treatment and provides valuable insights into viral infection mechanisms.
CELL CHEMICAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Lixue Huang, Qun Yao, Xiaoying Gu, Qiongya Wang, Lili Ren, Yeming Wang, Ping Hu, Li Guo, Min Liu, Jiuyang Xu, Xueyang Zhang, Yali Qu, Yanqing Fan, Xia Li, Caihong Li, Ting Yu, Jiaan Xia, Ming Wei, Li Chen, Yanping Li, Fan Xiao, Dan Liu, Jianwei Wang, Xianguang Wang, Bin Cao
Summary: The study aimed to compare the health outcomes and sequelae symptoms between 6 months and 12 months after discharge among COVID-19 survivors. Most survivors had good physical and functional recovery during the 1-year follow-up, but their health status at 12 months was still lower than that of the control population.
Article
Immunology
Danwei Yu, Yuanmei Zhu, Tao Jiao, Tong Wu, Xia Xiao, Bo Qin, Huihui Chong, Xiaobo Lei, Lili Ren, Sheng Cui, Jianwei Wang, Yuxian He
Summary: The study identified the critical role of the membrane-proximal external region (MPER) sequence of SARS-CoV-2 spike fusion protein in viral infectivity and developed novel lipopeptides with significantly improved inhibitory activity against SARS-CoV-2 fusion and infection. The new inhibitors also showed potent activity against emerging SARS-CoV-2 variants and cross-inhibited other human CoVs. Structural characterization revealed the mechanisms underlying the high binding and inhibition of the new inhibitors, providing important insights for peptide therapeutics development against SARS-CoV-2 and other CoVs.
EMERGING MICROBES & INFECTIONS
(2021)