4.2 Article

Informative microsatellites for genetic population studies of black-faced lion tamarins (Leontopithecus caissara)

Journal

GENETICS AND MOLECULAR BIOLOGY
Volume 34, Issue 1, Pages 173-175

Publisher

SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572010005000111

Keywords

lion tamarins; endangered species; genetic diversity; New World primate; SSR transferability

Funding

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [05/04346-2, 07/07409-0]
  2. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
  3. The Margot Marsh Biodiversity Foundation
  4. Rufford

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Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagui Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for genetic investigation in other congeneric species.

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