Article
Biotechnology & Applied Microbiology
Lili Du, Keanning Li, Tianpeng Chang, Bingxing An, Mang Liang, Tianyu Deng, Sheng Cao, Yueying Du, Wentao Cai, Xue Gao, Lingyang Xu, Lupei Zhang, Junya Li, Huijiang Gao
Summary: This study identified candidate genes affecting subcutaneous fat deposition in beef cattle through genomics and transcriptomics analysis. Significant SNPs within or near 11 genes associated with backfat thickness were identified. Additionally, 13 overlapping genes related to fat deposition were determined. Finally, seven biomarkers associated with fat accumulation were screened. These findings provide novel insights into the regulation mechanism underlying bovine fat accumulation.
Article
Biotechnology & Applied Microbiology
Lili Du, Keanning Li, Tianpeng Chang, Bingxing An, Mang Liang, Tianyu Deng, Sheng Cao, Yueying Du, Wentao Cai, Xue Gao, Lingyang Xu, Lupei Zhang, Junya Li, Huijiang Gao
Summary: This study conducted genomics and transcriptomics analysis to detect potential genes affecting subcutaneous fat deposition in beef cattle, identified important genes related to fat deposition, and ultimately screened out seven potential biomarkers. These findings provide new insights into the regulatory mechanism underlying bovine fat accumulation.
Article
Microbiology
Venkata Vinay Kumar Bandarupalli, Benoit St-Pierre
Summary: In ruminant livestock production, ruminal acidosis is caused by the high intake of starch-rich feed, leading to a state of subacute acidosis (SARA) and eventually acute acidosis due to lactate accumulation. This study identified two bacterial operational taxonomic units (OTUs) enriched from rumen fluid cultures, Bt-01708_Bf and Bt-01899_Ap, which can metabolize lactate into distinct subgroups based on other metabolic capabilities. The genomic analysis revealed the presence of lactate dehydrogenase, lactate transporter, and pathways for short chain fatty acids production and glycogen synthesis in these bacterial species.
Article
Multidisciplinary Sciences
Marco Antonio Perpetuo de Sousa, Flavia Regina Florencio de Athayde, Mariangela Bueno Cordeiro Maldonado, Andressa Oliveira de Lima, Marina Rufino S. Fortes, Flavia Lombardi Lopes
Summary: The study found that approximately 71.6% of the predicted miRNA targets were altered as a result of SNPs located within the seed region of the mature miRNAs, potentially affecting miRNA maturation. Analysis revealed that 48.6% of the sequences had values sufficient enough to alter miRNA maturation.
Article
Veterinary Sciences
Wei He, Xibi Fang, Xin Lu, Yue Liu, Guanghui Li, Zhihui Zhao, Junya Li, Runjun Yang
Summary: This study verified the relationship between the expression of ACSF3 and cellular triglyceride production, and analyzed the relationship between ACSF3 polymorphism and economic traits and fatty acid composition in Chinese Simmental cattle. ACSF3 plays a crucial role in regulating cellular triacylglycerol and long-chain polyunsaturated fatty acid levels, and polymorphism could serve as a useful molecular marker for marker-assisted selection in beef cattle breeding for intramuscular fat deposition traits.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Siyuan Wang, Jie Liu, Weiming Zhao, Guofu Wang, Shuxin Gao
Summary: This study examined the differences between BF and PF in Angus and Simmental cattle, revealing that BF has smaller cell area but greater lipid deposition ability. RNA-seq identified a large number of genes, indicating that genes related to fat metabolism were highly expressed in four tissues, and DEGs differed significantly between Angus and Simmental cattle.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Matthew A. Scott, Amelia R. Woolums, Cyprianna E. Swiderski, Andy D. Perkins, Bindu Nanduri, David R. Smith, Brandi B. Karisch, William B. Epperson, John R. Blanton
Summary: This study compared the blood transcriptomes of two distinct populations of post-weaned beef cattle, with and without developing bovine respiratory disease (BRD) within 28 days of arrival. A total of 132 unique differentially expressed genes (DEGs) were identified between groups stratified by disease severity, providing insights into potential biomarkers and functional mechanisms for predicting BRD development and severity. ROC curves generated from expression data of six specific genes showed good-to-excellent predictability for classifying disease occurrence and severity, highlighting their potential in clinical application for BRD management.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Huan Chen, Tao Peng, Hanle Shang, Xianglong Shang, Xianghui Zhao, Mingren Qu, Xiaozhen Song
Summary: Dietary supplementation with Puerarin enhances intramuscular fat deposition in heat-stressed beef cattle by regulating lipid metabolism pathways.
FRONTIERS IN NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Piera Mazzone, Antonella Di Paolo, Linda Petrucci, Martina Torricelli, Sara Corneli, Carla Sebastiani, Marcella Ciullo, Martina Sebastianelli, Silva Costarelli, Eleonora Scoccia, Fiorella Sbarra, Federica Gabbianelli, Giovanni Chillemi, Alessio Valentini, Giovanni Pezzotti, Massimo Biagetti
Summary: This preliminary study aimed to verify the association between MAP infectious status and the presence of single nucleotide polymorphisms (SNPs) in candidate genes in Italian Marchigiana beef cattle. A longitudinal study was conducted, analyzing DNA samples from 112 bovines and focusing on the differences in genotypic frequencies of the candidate genes IL10RA and IL12RB2. The results revealed potential resistance traits to PTB in the Marchigiana breed, as SNP genotypes associated with PTB resistance were found more frequently.
Article
Agriculture, Dairy & Animal Science
Farhad Bordbar, Mohammadreza Mohammadabadi, Just Jensen, Lingyang Xu, Junya Li, Lupei Zhang
Summary: Genome-wide association studies were used to identify SNPs and candidate genes related to carcass depth and hind leg circumference in Simmental beef cattle. Several potential SNPs and candidate genes, including CDKAL1 and E2F3, were identified, which can help breeders understand and improve meat yield.
Article
Agriculture, Dairy & Animal Science
Virginia Mara Pereira Ribeiro, Gabriela Canabrava Gouveia, Fabio Luiz Buranelo Toral
Summary: The measurement age of a longitudinal trait and pre-sampling procedures in beef cattle herds can influence the identification of functional candidate genes associated with a trait. Different genes may play a more significant role in body weight at various ages, and sequential sampling can affect the results of genome-wide association studies for longitudinal traits.
JOURNAL OF ANIMAL BREEDING AND GENETICS
(2023)
Article
Multidisciplinary Sciences
Lili Du, Tianpeng Chang, Bingxing An, Mang Liang, Xinghai Duan, Wentao Cai, Bo Zhu, Xue Gao, Yan Chen, Lingyang Xu, Lupei Zhang, Junya Li, Huijiang Gao
Summary: This study investigated the molecular mechanism underlying water holding capacity (WHC) in Chinese Simmental beef cattle by conducting RNA sequencing on longissimus dorsi (LD) muscles. Significant correlations were found between WHC and water loss, IMF content, and specific genes were identified as potential novel candidate genes affecting WHC. The findings offer important insight for understanding the regulation of WHC and improving beef quality.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Yiming Chang, Hua Yue, Cheng Tang
Summary: This study investigated the prevalence and molecular characteristics of BRSV in beef cattle with BRDC in China, revealing wide geographical distribution of BRSV in China, with subgroup III strains being dominant and showing unique evolutionary trends. This contributes to a better understanding of the prevalence and genetic evolution of BRSV.
Article
Biochemistry & Molecular Biology
Tianzhen Wang, Qunhao Niu, Tianliu Zhang, Xu Zheng, Haipeng Li, Xue Gao, Yan Chen, Huijiang Gao, Lupei Zhang, George E. Liu, Junya Li, Lingyang Xu
Summary: This study conducted an integrative analysis of gene-based GWAS and eQTL analysis to identify candidate genes for carcass yield traits in beef cattle. The results revealed shared genes that may play important roles in carcass yield traits and further demonstrated the effects of two genes, PON3 and PRIM2, on the proliferation and differentiation of bovine skeletal muscle satellite cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Samir Id-Lahoucine, Joaquim Casellas, Aroa Suarez-Vega, Pablo A. S. Fonseca, Flavio S. Schenkel, Mehdi Sargolzaei, Angela Canovas
Summary: This study analyzed the genomic data of 441,802 Holstein cattle and identified 604 chromosomal regions showing significant transmission ratio distortion (TRD). The majority of these regions exhibited allelic TRD patterns, with reduced viability for carrier offspring or lethality for homozygous individuals. Functional analysis also revealed candidate genes related to embryonic development, DNA repair, and meiotic processes. These findings have implications for improving breeding success in cattle.
Article
Agriculture, Dairy & Animal Science
Roberta Ferreira Leite, Joao Diego de Agostini Losano, Giulia Kiyomi Vechiato Kawai, Bruno Rui, Ken Kawaoka Nagai, Vivian Cardoso Castiglioni, Adriano Felipe Perez Siqueira, Mayra Elena Ortiz D'Avila Assumpcao, Pietro Sampaio Baruselli, Marcilio Nichi
Summary: This study used conventional and functional techniques to evaluate sperm samples from bulls with known fertility history, revealing associations between mitochondrial dysfunctions, oxidative stress, and damage to sperm structures in lesser fertility bulls. Greater fertility bulls demonstrated better sperm quality and indicators of functional cell structures. The importance of evaluating and correlating different sperm functions to understand the effects of distinct parameters on sperm fertilization capacity was highlighted.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Shahram Niknafs, Marina R. S. Fortes, Sungbo Cho, John L. Black, Eugeni Roura
Summary: This study investigated how amino acid preferences change with growth rates in broiler chickens. Differences in amino acid metabolism were found between chickens with extreme growth rates, with SG preferring NEAA and FG rejecting NEAA. Transcriptomic and proteomic analyses revealed genes and proteins associated with differences in growth rate.
Article
Agriculture, Dairy & Animal Science
Barbara M. Nascimento, Roberto Carvalheiro, Rodrigo de A. Teixeira, Laila T. Dias, Marina R. S. Fortes
Summary: This study evaluated the genotype x environment interaction for scrotal circumference measured at different ages in Brahman bulls. Weak evidence of GxE effect was found at 12 and 18 months, recommending these ages for selection. No changes in sire rankings were observed across environments, making selection more straightforward at these ages.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Cell Biology
Felipe Eduardo Luedke, Caroline Pereira da Costa, Camilla Mota Mendes, Thais Rose dos Santos Hamilton, Marcella Pecora Milazzotto, Mayra E. O. A. Assumpcao, Marcelo Demarchi Goissis
Summary: This study found that supplementation of fetal bovine serum (FBS) in embryonic culture medium can affect cell differentiation during early bovine embryo development. Absence of FBS reduces blastocyst rates, but there is no reduction when 33% volume renewal of the medium is done at 90 hours post insemination. Additionally, FBS supplementation alters the allocation of trophectoderm (TE) and primitive endoderm cells.
Article
Agriculture, Dairy & Animal Science
Bismarck M. Santiago, Welder A. Baldassini, Marcos R. Chiaratti, Anand K. Pandey, Juliana A. Torrecilhas, Rodrigo N. S. Torres, Richard Ribeiro, Dante P. D. Lanna, Guilherme L. Pereira, Rogerio A. Curi, Luis Artur L. Chardulo, Otavio R. Machado Neto
Summary: This study analyzed the impact of castration on the expression of genes in skeletal muscles and the quality of beef. Results showed that castrated steers had increased expression of genes related to fat metabolism compared to intact bulls. This led to higher intramuscular fat content and different fatty acid profiles in the beef produced by steers and bulls. Despite a 180-day feeding period, crossbred bulls did not exhibit substantial increases in intramuscular fat or improved fatty acid profile compared to steers.
Article
Biochemistry & Molecular Biology
German Dario Ramirez-Zamudio, Maria Julia Generoso Ganga, Guilherme Luis Pereira, Ricardo Perecin Nociti, Marcos Roberto Chiaratti, Reinaldo Fernandes Cooke, Luis Artur Loyola Chardulo, Welder Angelo Baldassini, Otavio Rodrigues Machado-Neto, Rogerio Abdallah Curi
Summary: The aim of this study was to analyze the effects of different diets on adipogenesis and lipogenesis in calves. It was found that calves receiving creep feeding had higher intramuscular fat content and marbling score compared to those without creep feeding. Differentially expressed genes related to fatty acid metabolism, cholesterol biosynthesis, and insulin sensitivity were identified.
Article
Agriculture, Dairy & Animal Science
Wellington B. Santos, Camila B. Pereira, Amanda M. Maiorano, Cherlynn D. Silva Arce, Welder A. Baldassini, Guilherme L. Pereira, Luis Artur L. Chardulo, Otavio R. M. Neto, Henrique N. Oliveira, Rogerio A. Curi
Summary: Significant progress has been made in the genetic improvement of livestock species, particularly through genomics and predicting breeding values. This study aims to estimate individual genomic inbreeding coefficient and identify runs of homozygosity and heterozygosity throughout the genome of Quarter Horse racing line. Moderately high genomic inbreeding was observed, with candidate genes related to crucial biological processes and muscle disorders identified. These findings have important implications for selecting animals with regenerative capacity and developing treatments for muscle disorders.
JOURNAL OF ANIMAL BREEDING AND GENETICS
(2023)
Article
Biochemical Research Methods
Bismarck Santiago, Welder Baldassini, Otavio Machado Neto, Luis Artur Chardulo, Rodrigo Torres, Guilherme Pereira, Rogerio Curi, Marcos Roberto Chiaratti, Pedro Padilha, Laura Alessandroni, Mohammed Gagaoua
Summary: This study investigated the skeletal muscle proteome of crossbred bulls and steers to explain the differences in carcass and meat quality traits. The results showed that steers had better meat quality, while bulls had higher carcass fatness. The proteomic analysis revealed differences in protein expression related to energy metabolism, catabolic processes, oxidative stress, and muscle contraction.
JOURNAL OF PROTEOMICS
(2023)
Article
Agriculture, Dairy & Animal Science
Rodrigo de Nazare Santos Torres, Larissa de Melo Coelho, Caren Paludo Ghedini, Otavio Rodrigues Machado Neto, Luis Artur Loyola Chardulo, Juliana Akamine Torrecilhas, Roberta de Lima Valenca, Welder Angelo Baldassini, Marco Tulio Costa Almeida
Summary: The objective of this study was to evaluate the effects of different nutritional strategies on reducing enteric methane emission in feedlot sheep using meta-analytical methods. The results showed that lipid inclusion in diets had the greatest effectiveness in reducing methane emission, followed by tannins. Rumen defaunation and essential oils inclusion had no effect on methane production. Further studies are needed to confirm the effects of saponins and nitrate supplementation on methane emission.
SMALL RUMINANT RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Rodrigo de Nazare Santos Torres, Joao Pedro Amaral Bertoco, Maria Carolina Goncalves de Arruda, Larissa de Melo Coelho, Josimari Regina Paschoaloto, Otavio Rodrigues Machado Neto, Jane Maria Bertocco Ezequiel, Marco Tulio Costa Almeida, Luis Artur Loyola Chardulo, Welder Angelo Baldassini
Summary: Molasses is commonly used in the diet of ruminant animals, but its effect on carcass parameters is not well-established. This study aimed to evaluate the impact of molasses inclusion in the diet of feedlot cattle on performance and carcass parameters. Thirteen peer-reviewed publications with 45 treatment means were analyzed, and the effect of molasses on beef cattle diets was assessed through weighted mean differences. The findings showed that the inclusion of molasses improved dry matter digestibility but reduced NDF digestibility, carcass weight, subcutaneous, and visceral fat. The level of molasses inclusion and the experimental period were the main factors influencing the responses.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Food Science & Technology
Welder Baldassini, Marcelo Coutinho, Gregori Rovadoscki, Bruna Menezes, Murilo Tagiariolli, Juliana Torrecilhas, Julia Leonel, Guilherme Pereira, Rogerio Curi, Otavio Machado Neto, Luis Artur Chardulo
Summary: This study compared the effects of hanging the carcass by the Achilles tendon (AS) versus pelvic suspension (PS) on meat quality traits. The results showed that PS improved the sensory tenderness of Nellore bulls and Brangus heifers. Moreover, PS method allowed for a reduction in aging time and could meet the demand of consumer markets for a certain level of eating quality.
Article
Biochemistry & Molecular Biology
Ariane Enara Pedro, Juliana Akamine Torrecilhas, Rodrigo Nazare Santos Torres, German Dario Ramirez-Zamudio, Welder Angelo Baldassini, Luis Artur Loyola Chardulo, Rogerio Abdallah Curi, Gustavo Henrique Russo, Juliane Arielly Napolitano, Gustavo Lucas Bezerra Tinoco, Thiago Barcaca Mariano, Jordana Luiza Caixeta, Philipe Moriel, Guilherme Luis Pereira
Summary: This study evaluated the effects of early weaning on the skeletal muscle metabolism of calves. Gene expression analysis revealed differences in gene expression related to lipid and fatty acid metabolism. These findings suggest that diet can influence energy metabolism and promote postnatal adipogenesis and lipogenesis.
Article
Agriculture, Dairy & Animal Science
Henrique Biasotto Morais, Luis Artur Loyola Chardulo, Welder Angelo Baldassini, Isabella Cristina de Castro Lippi, Gabriela Belinassi Orsi, Clandio Favarini Ruviaro
Summary: This study analyzed the environmental impacts and economic factors of a beef cattle feedlot system. Using a partial Life Cycle Assessment approach, it was found that feed production is the most important source of greenhouse gas emissions, and increased marbling degree in meat can lead to an increase in environmental impacts.
Review
Agriculture, Dairy & Animal Science
Carlos Alonso Paco Nagaki, Thais Rose dos Santos Hamilton, Mayra Elena Ortiz D Avila Assumpcao
Summary: The evaluation of sperm chromatin plays a crucial role in predicting bull reproductive capacity, although the physiological mechanisms of protamination are still poorly understood.
ANIMAL REPRODUCTION
(2022)
