Stress-Induced Mutation Rates Show a Sigmoidal and Saturable Increase Due to the RpoS Sigma Factor in Escherichia coli

Stress-induced mutagenesis was investigated in the absence of selection for growth fitness by using synthetic biology to control perceived environmental stress in Escherichia coli. We find that controlled intracellular RpoS dosage is central to a sigmoidal, saturable three- to fourfold increase in mutation rates and associated changes in DNA repair proteins.