Article
Biotechnology & Applied Microbiology
Kaori Itto-Nakama, Shun Watanabe, Shinsuke Ohnuki, Naoko Kondo, Ryota Kikuchi, Toru Nakamura, Wataru Ogasawara, Ken Kasahara, Yoshikazu Ohya
Summary: In this study, AI models were developed to predict ethanol yields in yeast fermentation cultures under high-sugar stress conditions using cell morphological data. The best performance was achieved by the neural network algorithm with an R2 value of 0.95, and it could predict ethanol yields up to 60 minutes in advance. Morphological data from cells cultured in low-glucose medium were not suitable for accurate prediction under high-glucose stress conditions. Feeding experiments revealed phase-dependent morphological changes. Monitoring yeast morphology under stress can aid in understanding intracellular physiological conditions and improve the management and stable production of desired biocommodities.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Multidisciplinary Sciences
George C. Kapetanakis, Luis Santos Sousa, Charlotte Felten, Loic Mues, Philippe Gabant, Laurence Van Nedervelde, Isabelle Georis, Bruno Andre
Summary: The growth of lactic acid bacteria is a common problem in bioethanol production, and yeast strains used in this process can support their proliferation by excreting amino acids. In this study, it was found that the deletion of a specific amino acid transporter gene reduced the ability of yeast to promote lactic acid bacteria growth. It is suggested that using yeast derivatives lacking this amino acid transporter gene may be a way to prevent bacterial contaminations during fermentation.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Environmental
Haoxuan Chen, Yunhao Zheng, Mingyu Wang, Yan Wu, Maosheng Yao
Summary: Breath-borne volatile organic compounds (VOCs) have been studied as biomarkers in medical diagnosis and environmental health research. In this study, the release of cellular VOCs from Saccharomyces cerevisiae after toxicant exposures and the underlying mechanism were investigated. The findings showed that different toxicant exposures caused distinctive VOC profiles in yeast cells. Ethyl acetate and ethyl n-propionate levels changed in response to all the toxicants tested, suggesting their potential for environmental toxicity monitoring. RNA-seq results revealed significant changes in metabolic and signaling pathways related to ribosome, carbohydrate, and amino acid metabolisms. These changes in metabolism may have contributed to the observed release of VOCs, such as ethyl acetate, from yeast cells. This study provides further evidence of the potential of VOCs released from living organisms for monitoring and protecting against toxic exposures, while also offering insights into the mechanistic changes in breath-borne VOCs observed in rats and humans exposed to air toxicants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Agricultural Engineering
Fengguang Zhao, Yaping Zhang, Jian Hu, Ce Shi, Xiang Ao, Shengding Wang, Ying Lin, Zhongwei Sun, Shuangyan Han
Summary: A haploid gene-deletion library was screened to identify 22 vanillin-tolerant mutants and investigate the tolerance mechanisms. The deleted genes in these mutants were found to be involved in phosphate and inositol polyphosphate metabolism and intracellular sterol transport. Activation of the phosphate signaling pathway was unfavorable for yeast under vanillin pressure, while inositol polyphosphates played a role in altering vanillin tolerance by regulating phosphate metabolism. Additionally, reducing sterol transport from the plasma membrane enhanced tolerance to vanillin. Two representative yeast deletions, pho84 Delta and lam3 Delta, showed good growth performance and rapid ethanol production in the presence of vanillin.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Shuangping Liu, Donglin Ma, Zhihui Li, Hailong Sun, Jieqi Mao, Ying Shi, Xiao Han, Zhilei Zhou, Jian Mao
Summary: This study assessed the types and amounts of YAN sources and genes regulating higher alcohol synthesis in a yeast used for huangjiu brewing. It found that NH4Cl was most effective in reducing higher alcohol content, providing insights for controlling huangjiu quality by adding NH4Cl.
Article
Green & Sustainable Science & Technology
Dagnew Bitew, Marye Alemu, Anteneh Tesfaye, Berhanu Andualem
Summary: This study aimed to isolate efficient ethanologenic microbes from Ethiopian traditional fermented beverages, evaluate their ethanol yield using banana peel as a substrate, and optimize fermentation conditions. The selected yeast strains Saccharomyces cerevisiae showed high tolerance to stress conditions and produced significant amounts of ethanol from banana peel waste. The results suggest that these strains are promising candidates for bioethanol production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2024)
Article
Cell Biology
Yoko Kagohashi, Michiko Sasaki, Alexander I. May, Tomoko Kawamata, Yoshinori Ohsumi
Summary: This study provides insights into the function and mechanism of Atg15 phospholipase, further understanding the disruption of autophagosome inner membrane structure.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhiling Kuang, Jiyuan Ke, Jiong Hong, Zhongliang Zhu, Liwen Niu
Summary: PCI domain proteins play important roles in post-transcriptional gene regulation. In this study, the structure of yeast Thp3-Csn12-Sem1 ternary complex was determined, revealing significant structural differences compared to the Sac3-Thp1-Sem1 complex. This structure provides a foundation for further exploring its specific recruitment to the spliceosome.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kunio Nakatsukasa, Munetaka Fujisawa, Xiaotan Yang, Tomoyuki Kawarasaki, Fumihiko Okumura, Takumi Kamura
Summary: This study found that Tgl4 is a stable protein that can be inactivated through dephosphorylation, playing an important regulatory role in the cell cycle.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Rostislav Boltyanskiy, Mary Ann Odete, Fook Chiong Cheong, Laura A. Philips
Summary: Total holographic characterization (THC) is an efficient, automated, label-free method for accurately identifying cell viability. It determines the size and index of refraction of individual particles using the Lorenz-Mie theory of light scattering, and can effectively distinguish living and dead cells by their refractive index.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Wilson J. F. Lemos, Renato L. Binati, Nicolo Bersani, Sandra Torriani
Summary: The study evaluated the ability of different non-Saccharomyces yeasts to produce glutathione, with Metschnikowia spp. showing the highest concentrations.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Guiping Gong, Yueping Zhang, Zibai Wang, Luo Liu, Shuobo Shi, Verena Siewers, Qipeng Yuan, Jens Nielsen, Xu Zhang, Zihe Liu
Summary: This study developed an efficient tool for genome disruption and single-nucleotide conversion, utilizing a gRNAtRNA array and SpCas9-NG (GTR 2.0). The tool achieved near 100% efficiency for gene disruptions in yeast cells covering all 16 possible NGN PAMs and all 12 possible single-nucleotide conversions (N to N). Furthermore, the tool was able to achieve 66.67% mutation efficiency for multiplexed single-nucleotide conversions and 100% mutation efficiency for generating multiple conversions in different genes simultaneously. GTR 2.0 will significantly enhance the scope, efficiency, and capabilities of yeast genome editing, serving as a versatile and valuable addition to the toolbox of synthetic biology and metabolic engineering.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Liyuan Cai, Jiangkun Yu, Rudy Hartanto, Desheng Qi
Summary: This study demonstrates that dietary supplementation with Saccharomyces cerevisiae, Clostridium butyricum, and their combination effectively improves rumen fermentation function and growth performance of heat-stressed goats.
Article
Biochemistry & Molecular Biology
Klaudia Kuczera, Katarzyna Naparlo, Miroslaw Soszynski, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Summary: Capsaicin (CAP) is a common food constituent in red peppers and has bactericidal and fungicidal activity. This study aimed to investigate the toxicity of CAP to Saccharomyces cerevisiae yeast and test whether oxidative stress plays a role. CAP showed good antioxidant properties but also generated hydrogen peroxide through autoxidation. CAP inhibited yeast growth, especially in mutants deficient in superoxide dismutase 1 or catalase T. However, oxidative stress did not mediate CAP's toxicity, as there was no increase in ROS levels. CAP caused a decrease in membrane fluidity, suggesting that disturbance of membrane properties is the main cause of CAP toxicity to yeast.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
I-Chen Chen, Yi-Ching Liu, Yen-Hsien Wu, Shih-Hsing Lo, Shu-Chi Wang, Chia-Yang Li, Zen-Kong Dai, Jong-Hau Hsu, Chung-Yu Yeh, Yu-Hsin Tseng
Summary: Pulmonary hypertension (PH) is a severe progressive disease caused by the uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs). The down-regulation of Mitofusin-2 (MFN2) is known to contribute to PH. In this study, it was found that hypoxia increases the viability and reduces the expression of MFN2 in PASMCs. However, MFN2 overexpression and the use of proteasome inhibitors restored MFN2 expression, inhibited hypoxia-induced proliferation, and induced the expression of cleaved-PARP1, suggesting their potential in improving the hypoxia-induced proliferation of PASMCs.