Association Between Polymorphism of the Tumor Necrosis Factor Alpha-308 Gene Promoter and Colon Cancer in the Chinese Population

Tumor necrosis factor (TNF) is a type of cytokine that inhibits tumorigenesis. Several studies have assessed the relationship between the polymorphism of TNF-alpha 308G/A and the susceptibility to colon cancer; however, the results have been controversial. A case-control study was carried out to investigate whether TNF-alpha 308G/A gene polymorphism was associated with the risk of colon cancer in a group of 180 cases and 180 controls from Heilongjiang, China. DNA was isolated from peripheral blood, and gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results of our study showed that colon cancer cases had a significantly higher frequency of TNF-alpha 308AA genotype (odds ratio = 9.42, 95% confidence interval = 1.18, 75.15; p = 0.03) than controls. When stratified by the tumor location, tumor size, growth pattern, differentiation, and stage of colon cancer, no statistically significant results was observed. The present study demonstrated that TNF-alpha 308AA genotype was associated with a higher risk of colon cancer in the Chinese population. Confirmation of these findings in other populations is required.