Journal
GENESIS
Volume 50, Issue 2, Pages 112-118Publisher
WILEY
DOI: 10.1002/dvg.20798
Keywords
mouse models of cancer; BAC recombineering; BAC transgenesis
Categories
Funding
- NCI NIH HHS [R01 CA130867, R01 CA130867-04] Funding Source: Medline
Ask authors/readers for more resources
The Notch1 receptor plays a critical role in cell fate decisions during development. Activation of Notch signaling has been implicated in several types of cancer, particularly T-cell acute lymphoblastic leukemia (T-ALL). Consequently, several transgenic mouse strains have been made to study the role of Notch1 in T-ALL. However, the existing Notch1 transgenic lines mimic a translocation event found in only similar to 1% of T-ALL cases. Here we describe three novel NOTCH1 transgenic mouse strains that have Cre-inducible expression of the entire human NOTCH1 locus, each possessing a common mutation found in T-ALL. Unlike existing Notch1 transgenic strains, these NOTCH1 transgenic strains express full-length receptors from anendogenous human promoter that should be susceptible to a number of Notch antagonists that have recently been developed. These strains will allow researchers to modulate Notch signaling to study bothnormal development and cancer biology. genesis 50:112118, 2012. (C) 2011 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available