Article
Multidisciplinary Sciences
Laurens H. Lindenburg, Teodors Pantelejevs, Fabrice Gielen, Pedro Zuazua-Villar, Maren Butz, Eric Rees, Clemens F. Kaminski, Jessica A. Downs, Marko Hyvonen, Florian Hollfelder
Summary: The study focused on the role of RAD51-interacting modules within BRCA2, creating chimeric repeats to measure binding to RAD51. Surprisingly, certain shuffled module combinations showed stronger binding than natural repeats, with weak correlation to affinities of natural repeats. The strongest chimera, BRC8-2, showed improved binding to RAD51 and functioned in preventing the formation of nuclear RAD51 foci in human cells after ionizing radiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Shun Liu, Hua Chen, Yan Yin, Defen Lu, Guoming Gao, Jie Li, Xiao-Chen Bai, Xuewu Zhang
Summary: This study reveals that the nuclear protein BCCIP alpha binds to FAM46 and inhibits its PAP activity, thus affecting RNA stability. Structural analysis shows that BCCIP alpha, despite sharing most of the sequence with BCCIPII, adopts a unique fold completely different from BCCIPII. The unique fold of BCCIP alpha underlies its interaction with and functional regulation of FAM46.
Article
Genetics & Heredity
Jeanette H. Sutherland, William K. Holloman
Summary: BRC is a conserved sequence motif in BRCA2 tumor suppressor protein family, which interacts with RAD51 in DNA repair. It consists of two tetrameric sequence modules with hydrophobic residues separated by an intervening spacer region. Brh2 in Ustilago maydis is the only fungal BRCA2 ortholog with a single BRC, while others contain multiple tandem repeats. Comparative sequence analysis identified BRCA2 orthologs in other fungal phyla. A biological assay system was developed to evaluate BRC functionality and the importance of conserved amino acid residues in DNA repair. Certain BRC mutant variants, called antimorphs, were found to cause more severe DNA repair phenotypes than the null.
Article
Multidisciplinary Sciences
Luke A. Greenhough, Chih-Chao Liang, Ondrej Belan, Simone Kunzelmann, Sarah Maslen, Monica C. Rodrigo-Brenni, Roopesh Anand, Mark Skehel, Simon J. Boulton, Stephen C. West
Summary: Homologous recombination is a vital process in life, involved in replication fork protection, DNA repair, and genetic exchange. Mutations in key recombination genes increase the risk of various cancers and Fanconi anemia. The RAD51 paralogues, including RAD51B, RAD51C, RAD51D, and XRCC2, play important roles in these processes, as demonstrated by their structure and function in the RAD51B-RAD51C-RAD51D-XRCC2 complex (BCDX2). BCDX2 stimulates RAD51 filament formation for recombinational DNA repair, crucial for tumor avoidance.
Article
Biotechnology & Applied Microbiology
Claire Milsted, Bo Dai, Nelson Garcia, Lu Yin, Yan He, Shahryar Kianian, Wojciech Pawlowski, Changbin Chen
Summary: This study used phage display to investigate the interactions of maize RAD51A1 with other proteins. The results revealed several peptide sequences that bind to ZmRAD51A1, supporting a potential role for ZmRAD51A1 in transcriptional regulation and plant defense.
Article
Biochemistry & Molecular Biology
Jakub Muraszko, Karol Kramarz, Bilge Argunhan, Kentaro Ito, Gabriela Baranowska, Yumiko Kurokawa, Yasuto Murayama, Hideo Tsubouchi, Sarah Lambert, Hiroshi Iwasaki, Dorota Dziadkowiec
Summary: Rrp1 plays a role in modulating Rad51 function by alleviating toxicity associated with excessive Rad51 levels through its ATPase domain. It interacts directly with Rad51, removes it from double-stranded DNA, and has E3 ubiquitin ligase activity with Rad51 as a substrate, suggesting a multi-tiered regulation of Rad51 by Rrp1.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Veterinary Sciences
Zida Zhu, Taisuke Kitano, Masami Morimatsu, Kazuhiko Ochiai, Toshina Ishiguro-Oonuma, Kosuke Oosumi, Xianghui Lin, Koichi Orino, Yasunaga Yoshikawa
Summary: BRCA2 mutations are associated with mammary tumors in dogs and humans. The highly conserved region (HCR) near the BRC repeats of BRCA2 regulates its interaction with RAD51. HCR mutations may contribute to tumorigenesis in both species.
VETERINARY SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mathieu Lussier-Price, Haytham M. Wahba, Xavier H. Mascle, Laurent Cappadocia, Veronique Bourdeau, Christina Gagnon, Sebastian Igelmann, Kazuyasu Sakaguchi, Gerardo Ferbeyre, James G. Omichinski
Summary: The N-terminal region of SUMO proteins plays an auto-inhibition role in promoting PML-NB formation, and this auto-inhibition can be relieved by zinc, leading to increased PML-NB formation within cells.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Immunology
Adeline W. W. Chang, Scot E. E. Dowd, Gordon Brackee, Joe A. A. Fralick, Govindsamy Vediyappan
Summary: Staphylococcus aureus is an opportunistic pathogen that can cause various infections, including severe diseases such as pneumonia and sepsis. It can form biofilms on different surfaces and has an innate resistance to antibiotics and host defenses. Infections associated with biofilms can result in longer hospitalizations, surgeries, and even death. A small cyclic peptide called gurmarin, identified from a plant source, has been found to inhibit the formation of S. aureus biofilms in vitro and in vivo without affecting bacterial viability. The peptide shows potential for therapeutic use.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuyi Lu, Mingjuan Ou, Qianwen Ye, Xin Tong, Yuxing Guo, Daodong Pan, Zhen Wu
Summary: In this study, a bifunctional protein named LPxT-GYLEQ was developed by replacing the C-terminal hydrophobic domain with a synthetic antioxidant peptide. The LPxT-GYLEQ protein showed enhanced antioxidant activity in H2O2-damaged HepG2 cells and exhibited good gastrointestinal tolerance function. The protein also demonstrated strong adhesion in an intestinal cell model, offering a novel strategy for the protection of antioxidant peptides in oral delivery systems.
PROCESS BIOCHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Na Li, Ying Ma, Niuniu Chen, Xiaoyu Tang, Shanshan Lv
Summary: This study investigates the effect of microwave on the stability of peptide bonds in silk protein, and finds that microwave irradiation causes minor damage and negligible cleavage of the peptide bonds compared to conventional heating methods, making it suitable for silk protein dissolution.
ADVANCED FIBER MATERIALS
(2022)
Article
Veterinary Sciences
Kei Shimakawa, Kazuhiko Ochiai, Sachi Hirose, Eri Tanabe, Masaki Michishita, Motoharu Sakaue, Yasunaga Yoshikawa, Masami Morimatsu, Tsuyoshi Tajima, Masami Watanabe, Yoshikazu Tanaka
Summary: This study investigated the relationship between RAD51 expression and radiosensitivity in canine mammary tumor cell lines. The results showed that CHMm, with high RAD51 expression, was more sensitive to radiation than CHMp. Additionally, CHMp cells formed functional RAD51 foci and induced cell cycle arrest after irradiation, while CHMm cells did not.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Madison B. Adolph, Taha M. Mohamed, Swati Balakrishnan, Chaoyou Xue, Florian Morati, Mauro Modesti, Eric C. Greene, Walter J. Chazin, David Cortez
Summary: RAD51 and RADX play crucial roles in DNA repair and replication, regulating the stability of the genome by controlling the activity of RAD51. RADX inhibits RAD51 activity and stabilizes replication forks. BRCA2 can overcome RADX-mediated inhibition of RAD51.
Review
Physiology
Jinseung Jeong, Inhwan Hwang, Dong Wook Lee
Summary: The chloroplast proteome of plants consists of proteins encoded by both the chloroplast genome and the nuclear genome. Nuclear-encoded chloroplast proteins are synthesized in the cytosol and targeted to the chloroplasts post-translationally. Transit peptides play a crucial role in this import process by interacting with the TOC/TIC translocons.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mengfan Tang, Zhen Chen, Chao Wang, Xu Feng, Namsoo Lee, Min Huang, Huimin Zhang, Siting Li, Yun Xiong, Junjie Chen
Summary: ASF1 associates with RIF1 and regulates its functions in the DNA damage response, restoring homologous recombination in BRCA1-deficient cells and decreasing telomere fusion in TRF2-depleted cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Martin R. Higgs, Koichi Sato, John J. Reynolds, Shabana Begum, Rachel Bayley, Amalia Goula, Audrey Vernet, Karissa L. Paquin, David G. Skalnik, Wataru Kobayashi, Minoru Takata, Niall G. Howlett, Hitoshi Kurumizaka, Hiroshi Kimura, Grant S. Stewart
Article
Biochemistry & Molecular Biology
Shinichi Machida, Yoshimasa Takizawa, Masakazu Ishimaru, Yukihiko Sugita, Satoshi Sekine, Jun-ichi Nakayama, Matthias Wolf, Hitoshi Kurumizaka
Article
Biochemistry & Molecular Biology
Yusuke Okamoto, Watal M. Iwasaki, Kazuto Kugou, Kazuki K. Takahashi, Arisa Oda, Koichi Sato, Wataru Kobayashi, Hidehiko Kawai, Ryo Sakasai, Akifumi Takaori-Kondo, Takashi Yamamoto, Masato T. Kanemaki, Masato Taoka, Toshiaki Isobe, Hitoshi Kurumizaka, Hideki Innan, Kunihiro Ohta, Masamichi Ishiai, Minoru Takata
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Di Luo, Daiki Kato, Jumpei Nogami, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Hidetoshi Kono
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Akihisa Osakabe, Zdravko J. Lorkovic, Wataru Kobayashi, Hiroaki Tachiwana, Ramesh Yelagandula, Hitoshi Kurumizaka, Frederic Berger
NUCLEIC ACIDS RESEARCH
(2018)
Article
Multidisciplinary Sciences
Akihito Harada, Kazumitsu Maehara, Yusuke Ono, Hiroyuki Taguchi, Kiyoshi Yoshioka, Yasuo Kitajima, Yan Xie, Yuko Sato, Takeshi Iwasaki, Jumpei Nogami, Seiji Okada, Tetsuro Komatsu, Yuichiro Semba, Tatsuya Takemoto, Hiroshi Kimura, Hitoshi Kurumizaka, Yasuyuki Ohkawa
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Yoshimasa Takizawa, Hiroki Tanaka, Shinichi Machida, Masako Koyama, Kazumitsu Maehara, Yasuyuki Ohkawa, Paul A. Wade, Matthias Wolf, Hitoshi Kurumizaka
Article
Biochemical Research Methods
Yasuhiro Arimura, Tomonori Kono, Kuniki Kino, Hitoshi Kurumizaka
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Kana Tanabe, Jiaan Liu, Daiki Kato, Hitoshi Kurumizaka, Kenzo Yamatsugu, Motomu Kanai, Shigehiro A. Kawashima
SCIENTIFIC REPORTS
(2018)
Correction
Biochemistry & Molecular Biology
Wakana Iwasaki, Yuta Miya, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroaki Tachiwana, Takehiko Shibata, Wataru Kagawa, Hitoshi Kurumizaka
Article
Cell Biology
Akihito Harada, Kazumitsu Maehara, Tetsuya Handa, Yasuhiro Arimura, Jumpei Nogami, Yoko Hayashi-Takanaka, Katsuhiko Shirahige, Hitoshi Kurumizaka, Hiroshi Kimura, Yasuyuki Ohkawa
NATURE CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Tomoya Kujirai, Haruhiko Ehara, Yuka Fujino, Mikako Shirouzu, Shun-ichi Sekine, Hitoshi Kurumizaka
Article
Multidisciplinary Sciences
Tomoya Kujirai, Christian Zierhut, Yoshimasa Takizawa, Ryan Kim, Lumi Negishi, Nobuki Uruma, Seiya Hirai, Hironori Funabiki, Hitoshi Kurumizaka
Review
Cell Biology
Tomoya Kujirai, Haruhiko Ehara, Shun-ichi Sekine, Hitoshi Kurumizaka
Summary: In eukaryotes, genomic DNA is packaged into chromatin and the nucleosome acts as a barrier to transcription. The RNA polymerase II elongation complex disassembles the nucleosome during transcription to allow passage. After transcription, the nucleosome is rebuilt by transcription-coupled nucleosome reassembly, which plays a crucial role in preserving epigenetic information. The histone chaperone FACT is involved in nucleosome disassembly, maintenance, and reassembly during transcription in chromatin. Recent structural studies have provided insights into the structural transitions of the nucleosome during transcription.
Article
Multidisciplinary Sciences
Masahiro Nishimura, Yoshimasa Takizawa, Kayo Nozawa, Hitoshi Kurumizaka
Summary: The study reveals the structure of p53 binding to a nucleosome, providing valuable insights into the mechanism by which p53 binds to its target DNA and changes the chromatin structure for gene activation.