Article
Cell Biology
Yuan Gao, Xue-Yan He, Xiaoli S. Wu, Yu-Han Huang, Shushan Toneyan, Taehoon Ha, Jonathan J. Ipsaro, Peter K. Koo, Leemor Joshua-Tor, Kelly M. Bailey, Mikala Egeblad, Christopher R. Vakoc
Summary: The transcriptional repressor ETV6 competes with EWS-FLI1 for binding to DNA elements, leading to disrupted transcription regulation and accelerated Ewing sarcoma development. Inactivating ETV6 allows EWS-FLI1 to hyper-activate these cis-elements, promoting mesenchymal differentiation and growth of the tumor.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Sidra Mumtaz, Muhammad Usman Rashid, Rizwan Ullah Khan, Naila Malkani
Summary: This study found that miR-4482 and miR-3912 can suppress ERG expression and its downstream target genes, thereby halting the progression of prostate cancer. These miRNAs may be employed as a potential therapeutic target for miRNA-based therapy against prostate cancer.
Article
Biochemistry & Molecular Biology
Zeyu Chen, Eri Arai, Omar Khan, Zhen Zhang, Shin Foong Ngiow, Yuan He, Hua Huang, Sasikanth Manne, Zhendong Cao, Amy E. Baxter, Zhangying Cai, Elizabeth Freilich, Mohammed A. Ali, Josephine R. Giles, Jennifer E. Wu, Allison R. Greenplate, Mohamed A. Hakeem, Qingzhou Chen, Makoto Kurachi, Kito Nzingha, Viktoriya Ekshyyan, Divij Mathew, Zhuoyu Wen, Nancy A. Speck, Alexis Battle, Shelley L. Berger, E. John Wherry, Junwei Shi
Summary: Improving the effector activity of antigen-specific T cells is a major goal in cancer immunotherapy. Although several effector T cell-driving transcription factors have been identified, the transcriptional coordination of T-EFF biology remains poorly understood. This study shows that genetic deletion of Fli1 enhances T-EFF responses by restraining T-EFF lineage differentiation.
Article
Biology
Perihan Yagmur Guneri-Sozeri, Gulden Ozden-Yilmaz, Asli Kisim, Ece Cakiroglu, Aleyna Eray, Hamdiye Uzuner, Gokhan Karakulah, Devrim Pesen-Okvur, Serif Senturk, Serap Erkek-Ozhan
Summary: Bladder cancer, with the form of urothelium carcinoma, causes more than 150,000 deaths annually. The histopathological classification of muscle-invasive (MIBC) and non-muscle-invasive (NMIBC) is crucial in affecting the prognosis and progression of the disease. This study utilized H3K27ac ChIP-seq to define the regulatory landscape of MIBC and NMIBC, and integrated the findings with existing data. The analysis revealed FRA1 and FLI1 as key transcription factors that differentially regulate the MIBC regulatory landscape.
COMMUNICATIONS BIOLOGY
(2023)
Article
Engineering, Biomedical
Jung-Kyu Han, Youngchul Shin, Min-Hwan Sohn, Saet-Byeol Choi, Dasom Shin, Youngrang You, Jong-Yeon Shin, Jeong-Sun Seo, Hyo-Soo Kim
Summary: Direct conversion of aHDFs into functional ECs was achieved by delivering ER71, KLF2, and TAL1, along with two EMT inhibitors, followed by a second-stage conversion process, resulting in improved efficiency. The genetic and epigenetic profiles of the converted iECs resembled those of functional ECs.
Article
Rheumatology
Peter E. Lipsky, Ronald Van Vollenhoven, Thomas Doerner, Victoria P. Werth, Joan T. Merrill, Richard Furie, Milan Petronijevic, Benito Velasco Zamora, Maria Majdan, Fedra Irazoque-Palazuelos, Robert Terbrueggen, Nikolay Delev, Michael Weiswasser, Shimon Korish, Mark Stern, Sarah Hersey, Ying Ye, Allison Gaudy, Zhaohui Liu, Robert Gagnon, Shaojun Tang, Peter H. Schafer
Summary: The pharmacodynamics and pharmacokinetics of oral iberdomide were evaluated in patients with active systemic lupus erythematosus (SLE). The results showed that iberdomide significantly modulated the patients' white blood cells and cytokines, and selectively reduced certain gene signatures, leading to clinical efficacy in patients with specific gene characteristics.
ANNALS OF THE RHEUMATIC DISEASES
(2022)
Article
Clinical Neurology
Erika De Francesco, Michele Terzaghi, Elisa Storelli, Luca Magistrelli, Cristoforo Comi, Massimiliano Legnaro, Marco Mauri, Franca Marino, Maurizio Versino, Marco Cosentino
Summary: The study revealed a distinct molecular signature in CD4+ T cells of idiopathic rapid eye movement sleep behavior disorder subjects, resembling cells from PD patients, suggesting early involvement of peripheral immunity in PD.
MOVEMENT DISORDERS
(2021)
Article
Biochemistry & Molecular Biology
Xinan H. Yang, Andrew Goldstein, Yuxi Sun, Zhezhen Wang, Megan Wei, Ivan P. Moskowitz, John M. Cunningham
Summary: Single-cell transcriptome analysis is crucial for understanding cellular state transitions. BioTIP, utilizing tipping-point theory and feature selection, overcomes challenges in identifying critical transition signals and inferring lineage-determining transcription factors.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Benjamin P. Song, Michelle F. Ragsac, Krissie Tellez, Granton A. Jindal, Jessica L. Grudzien, Sophia H. Le, Emma K. Farley
Summary: By conducting high-throughput screens on genomic elements in developing Ciona embryos, this study identifies an enhancer near Lama gene that is critical for notochord development. Transcription factors Zic and ETS regulate enhancer activity and their binding sites are conserved in Lama1 introns in mouse and human. Furthermore, a similar binding site logic is observed in other Ciona and vertebrate Bra enhancers. The study uncovers the importance of enhancer grammar and discovers conserved signatures of enhancer logic and grammar in chordates.
Article
Plant Sciences
Fan Qi, Fu Wang, Chunxiao Xiaoyang, Zhenhui Wang, Yujie Lin, Zhanwu Peng, Jun Zhang, Ningning Wang, Jian Zhang
Summary: This study conducted transcriptome analysis to analyze the gene expression profiles of different organs in flax. The results identified differentially expressed genes and organ-specific expression patterns. Gene ontology enrichment analysis revealed the biological processes and molecular functions associated with organ growth. The study also investigated the role of AP2 transcription factors in regulating flax organ development and stress response. Overall, this research provides valuable insights into the molecular mechanisms of flax organ development and has implications for genetic improvement of flax crops.
Review
Biochemistry & Molecular Biology
Mengyuan Wang, Qian Li, Lingbo Liu
Summary: Gene expression regulation involves multiple processes and various regulatory factors. This review provides an overview of key factors involved in gene expression regulation, such as transcription factors, chromatin accessibility, histone modifications, DNA methylation, and RNA modifications. Additionally, methods for detecting these regulatory factors are also described.
Article
Genetics & Heredity
Qiao Liu, Kui Hua, Xuegong Zhang, Wing Hung Wong, Rui Jiang
Summary: DeepCAGE is a deep learning framework that accurately predicts chromatin accessible regions in a variety of cell types, and exhibits superior performance in the classification and regression of chromatin accessibility signals.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Cell Biology
Granton A. Jindal, Alexis T. Bantle, Joe J. Solvason, Jessica L. Grudzien, Agnieszka D'Antonio-Chronowska, Fabian Lim, Sophia H. Le, Benjamin P. Song, Michelle F. Ragsac, Adam Klie, Reid O. Larsen, Kelly A. Frazer, Emma K. Farley
Summary: Transcriptional enhancers play a crucial role in directing precise gene expression patterns during development. Suboptimal or low-affinity binding sites are necessary for precise gene expression during heart development. Single-nucleotide variants (SNVs) can optimize the affinity of ETS binding sites and lead to gain-of-function (GOF) gene expression and phenotypic changes.
DEVELOPMENTAL CELL
(2023)
Review
Endocrinology & Metabolism
Robert S. Viger, Karine de Mattos, Jacques J. Tremblay
Summary: Understanding how genes are turned on and off is crucial for the development and function of different cell types. Transcription factors, such as the GATA family, play essential roles in controlling gene expression in the male gonads, impacting testis development, fetal testis gene expression, testicular disease, and XY sex differentiation in humans.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Caixia Hou, Abhisek Mandal, Juergen Rohr, Oleg Tsodikov
Summary: The ETS family transcription factors ERG and FLI1 play a key role in oncogenesis of prostate cancer and Ewing sarcoma, while MTM functions as an effective antagonist of ERG and FLI1. Studies of crystal structures and DNA binding reveal the disruption mechanism of MTM analogues on the interaction of ERG and FLI1.
Article
Health Policy & Services
Sheila L. Mason, Katherine E. Chike-Harris, Bettina M. Gyr, Emily Johnson
JOURNAL OF PEDIATRIC HEALTH CARE
(2019)
Article
Biochemistry & Molecular Biology
Tamara K. Nowling, Jennifer Dziadyk Fulton, Katherine Chike-Harris, Gary S. Gilkeson
MOLECULAR IMMUNOLOGY
(2008)
Review
Health Care Sciences & Services
Katherine E. Chike-Harris, Cathy Durham, Ayaba Logan, Gigi Smith, Ragan DuBose-Morris
Summary: A thorough literature review reveals the lack of standardized approach to integrating telehealth education into healthcare training programs. Different studies show inconsistency in the depth and breadth of telehealth content incorporated into curricula, highlighting the need for national competencies and standardized curriculum development in this area.
TELEMEDICINE AND E-HEALTH
(2021)
Article
Environmental Sciences
Michelle Nichols, Ronald Teufel, Sarah Miller, Mohan Madisetti, Christine San Giovanni, Katherine Chike-Harris, Lacy Jones, Margaret Prentice, Kenneth Ruggiero, Teresa Kelechi
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2020)
Article
Nursing
Carolyn M. Rutledge, Jennifer O'Rourke, Anne M. Mason, Katherine Chike-Harris, Lyn Behnke, Lolita Melhado, Loureen Downes, Tina Gustin
Summary: This article describes the development of telehealth competencies for education and practice using the Four P's of Telehealth framework. A modified Delphi technique was used to identify, develop, and evaluate telehealth competencies. The competencies were aligned with telehealth domains, expected activities, and outcomes to guide curriculum development, practice, and future research.
Article
Nursing
Katherine E. Chike-Harris
Summary: Telehealth education is recommended for nurse practitioners to increase their confidence in technology. This article provides an overview of how telehealth was integrated into an NP program of study.
JNP-JOURNAL FOR NURSE PRACTITIONERS
(2021)
Article
Nursing
Katherine E. Chike-Harris, Kelli Garber, Anne Derouin
Summary: The article highlights the need for graduate nursing students to acquire skills and competencies in telehealth, but acknowledges the lack of training in many nursing programs. To overcome barriers, it suggests that nursing faculty should use resources and the experiences of early adopters of telehealth to integrate adequate telehealth preparation into all graduate nursing programs.
Article
Education & Educational Research
Katherine E. Chike-Harris, Elizabeth Harmon, Kathryn van Ravenstein
NURSING EDUCATION PERSPECTIVES
(2020)
Article
Pediatrics
Kimberly R. Leslie, Katherine Chike-Harris
CLINICAL PEDIATRICS
(2018)
Meeting Abstract
Rheumatology
John L. Svenson, Katherine Chike-Harris, May Amria, Tamara Nowling
ARTHRITIS AND RHEUMATISM
(2008)