Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 169, Issue 3, Pages 192-196Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2010.09.014
Keywords
Orexin; Cloning; Skate; Elasmobranch; Feeding; Neuropeptide
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
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A 815 base pairs (bp) cDNA encoding for preproorexin (preproOX) was cloned in winter skate, a cartilaginous fish. Winter skate preproOX is 159 amino acids (aa) long and contains a 34 aa orexin A and 28 aa orexin B. The amino acid sequence of winter skate preproOX is more similar to tetrapod preproOXs (36-40% identity) than teleost preproOXs (23-33% identity). Whereas orexin B appears relatively well conserved among vertebrates, orexin A displays more variability, in particular due to an insertion sequence that is present in teleost fish, but not in skate and tetrapods. RT-PCR studies show that preproOX mRNA has a widespread distribution within the brain and is present in several peripheral tissues, including gastrointestinal tract, heart and testes. Fasting induced increases in preproOX expression in the hypothalamus, suggesting that orexin might play a role in the regulation of food intake in winter skate. (C) 2010 Elsevier Inc. All rights reserved.
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