Article
Cell Biology
Dong-Dong Wu, Sheng Jin, Ruo-Xiao Cheng, Wen-Jie Cai, Wen-Long Xue, Qing-Qing Zhang, Le-Jie Yang, Qi Zhu, Meng-Yao Li, Ge Lin, Yi-Zhen Wang, Xue-Pan Mu, Yu Wang, Igor Ying Zhang, Qi Zhang, Ying Chen, Sheng-Yang Cai, Bo Tan, Ye Li, Yun-Qian Chen, Pu-Juan Zhang, Chen Sun, Yue Yin, Ming-Jie Wang, Yi-Zhun Zhu, Bei-Bei Tao, Jia-Hai Zhou, Wei-Xue Huang, Yi-Chun Zhu
Summary: This study investigates the possibility of a mechanism beyond post-translational modifications to regulate protein function. It shows that hydrogen sulfide (H2S) can bind to the active-site copper of Cu/Zn-SOD, enhancing electrostatic forces and facilitating electron transfer. The physiological relevance of this H2S effect is demonstrated in in vitro and in vivo models where the cardioprotective effects of H2S depend on Cu/Zn-SOD.
Article
Nanoscience & Nanotechnology
Xiangshi Sun, Kongtong Yu, Yulin Zhou, Shiyan Dong, Wenji Hu, Yating Sun, Yuhuan Li, Jing Xie, Robert J. Lee, Fengying Sun, Yifan Ma, Shengnian Wang, Betty Y. S. Kim, Yifan Wang, Zhaogang Yang, Wen Jiang, Youxin Li, Lesheng Teng
Summary: The newly synthesized amphiphilic polymers can self-assemble into micellar nanoparticles with favorable hydrolysis kinetics, biocompatibility, and long circulation time. These materials not only exhibit better inflammation-targeting effects, but also do not induce an acidic microenvironment during degradation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biology
ShylajaNaciyar Mohandass, Mangalalakshmi Ragavan, Dineshbabu Gnanasekaran, Uma Lakshmanan, Prabaharan Dharmar, Sushanta Kumar Saha
Summary: Synthetic azo dyes are widely used but their improper discharge poses a threat to the environment. Genetically modified cyanobacteria with enhanced H2O2 accumulation through SOD overexpression can effectively degrade these dyes, suggesting a valuable bioremediation strategy.
Article
Biochemistry & Molecular Biology
Bindu Chandrasekharan, Claudia Montllor-Albalate, Alyson E. Colin, Joshua L. Andersen, Young C. Jang, Amit R. Reddi
Summary: Cu/Zn superoxide dismutase (Sod1) catalyzes the breakdown of superoxide radicals into oxygen and hydrogen peroxide, which acts as a signaling molecule. In yeast, Sod1 is involved in a redox signaling pathway that connects nutrient availability to energy metabolism control. This pathway stabilizes plasma membrane kinases Yck1/2, promoting glycolytic growth and repressing respiration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Plant Sciences
Li Tian, Junjiao Li, Caimin Huang, Dandan Zhang, Yan Xu, Xingyong Yang, Jian Song, Dan Wang, Nianwei Qiu, Dylan P. G. Short, Patrik Inderbitzin, Krishna Subbarao, Jieyin Chen, Xiaofeng Dai
Summary: The accumulation of ROS is a common defense mechanism in higher plants against pathogen attack. Previous studies showed that Cu/Zn-SOD1 (VdSOD1) in plants is upregulated following infection, indicating its role in pathogen virulence. Deletion of VdSOD1 leads to increased sensitivity to ROS generator menadione and relies on Golgi reassembly stacking protein for secretion. Overall, VdSOD1 plays a significant role in scavenging ROS and promoting virulence in V. dahliae.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ahmet Can Timucin, Suleyman Selim Cinaroglu, Osman Ugur Sezerman, Emel Timucin
Summary: The metallation status of human Cu/Zn superoxide dismutase 1 (SOD1) is crucial in the development of amyotrophic lateral sclerosis (ALS). Mutations at the ALS-linked positions H63R and K136A significantly affect dimer dynamics, leading to demetallation and potential ALS toxicity. This study highlights the potential use of atomistic simulations in studying disease variants related to SOD1.
FRONTIERS IN CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Xiaowen Wang, Hong Zhang, Russell Sapio, Jun Yang, Justin Wong, Xin Zhang, Jessie Y. Guo, Sharon Pine, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian, Dimitri G. Pestov, X. F. Steven Zheng
Summary: The study reveals a crucial role of nuclear SOD1 in ribosome biogenesis and proliferation in KRAS-driven lung cancer.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Chemical
Diana A. Fernandes, Paula Leandro, Eunice Costa, M. Luisa Corvo
Summary: This study investigated the effects of spray drying parameters on the properties of a Cu,Zn-SOD-based DPI powder, finding that the powders generated under different conditions exhibited fine particle fractions and high enzyme activity retention without loss of protein conformational stability. The technology proved suitable for preparing DPI powders containing Cu,Zn-SOD within the specified working ranges.
Article
Multidisciplinary Sciences
Anuradha Gautam, Fatima Nazish Khan, Surabhi Priya, Krishan Kumar, Shivani Sharda, Tanushri Kaul, Ishwar Singh, Sapna Langyan, Pranjal Yadava
Summary: Maize, an important crop, exhibits high drought tolerance, which may be attributed to the stress-tolerant properties of its SOD2 protein. We cloned and analyzed the Sod2 gene from the drought-tolerant HKI 335 maize inbred line. Comparative modeling revealed the similarity between HKI 335 SOD2 and a thermostable SOD from Potentilla atrosanguinea.
Article
Neurosciences
Natalia Lopez-Gonzalez del Rey, Miguel Angel Garcia-Cabezas
Summary: This review article summarizes the developmental, cellular, structural, and connectional features of the primate striatum and relates them to patterns of neurodegeneration in humans and non-human primate models. By understanding the primate anatomy and its relation to human pathology, new insights into the molecular, cellular, and connectional factors underlying the degeneration of striatal neurons and circuits can be gained. The article provides an overview of the types of neurons, cyto-, myelo-, and chemoarchitecture, developmental origin, organization of corticostriatal projections, and the topography and time-course of neurodegenerative diseases in both humans and non-human primates.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Karen E. Butler, Yoshihiko Takinami, Adam Rainczuk, Erin S. Baker, Blaine R. Roberts
Summary: Native mass spectrometry combined with IMS-MS technology can be used to study the tertiary and quaternary structure of proteins, validate other structural determination methods, and provide more comprehensive structural information.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Suffora Akhter, Salman Khursheed, Farukh Arjmand, Sartaj Tabassum
Summary: In this study, bio-compatible water-soluble conjugates of Co(II), Cu(II), and Zn(II) were synthesized and characterized. The spectroscopic techniques and crystallography confirmed the structure of the complexes. The DNA binding interaction studies revealed the mode of binding, DNA cleavage, chemical shift changes, EPR parameter alterations, SOD mimetic activity, and cytotoxicity of the complexes. Molecular docking studies further confirmed the electrostatic interaction of the complexes with DNA groove region.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Angelique N. Besold, Vinit Shanbhag, Michael J. Petris, Valeria C. Culotta
Summary: Copper is essential for all organisms, with ceruloplasmin being the most abundant extracellular copper-binding molecule in serum. Studies suggest that ceruloplasmin may be a preferred source of copper for fungal pathogens.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Chemistry, Physical
Mingju Lu, Jialu Wang, Guoyuan Ren, Fengjuan Qin, Zhiqiang Zhao, Kai Li, Wenxing Chen, Yuqing Lin
Summary: In this study, highly active nanozymes with excellent SOD-mimicking properties were prepared through a simple and low-cost method using copper foam and graphene oxide. These nanozymes exhibit higher catalytic activity compared to natural enzymes and do not possess typical enzyme mimic properties found in other nanomaterials. Furthermore, they successfully eliminate active oxygen in cigarette smoke, showing promise in the treatment of lung injury and inflammatory diseases related to free radical production.
Article
Biotechnology & Applied Microbiology
Jun Lin, Wenqian Jiang, Lin Chen, Huilian Zhang, Yang Shi, Xin Liu, Weiwen Cai
Summary: Banknotes can serve as a valuable genetic resource for discovering novel microbial gene sequences, including potentially economically valuable enzymes. High-throughput genomic sequencing technology was used to characterize the microbial diversity on US dollars and Chinese Renminbis, leading to the discovery of a significant number of novel gene sequences, one of which was experimentally verified for its catalytic activity.
Article
Cell Biology
Sara Al-Ghadban, Zaidmara T. Diaz, Hallie J. Singer, Karya B. Mert, Bruce A. Bunnell
Article
Cell Biology
Mark A. A. Harrison, Rachel M. Wise, Brooke P. Benjamin, Emily M. Hochreiner, Omair A. Mohiuddin, Bruce A. Bunnell
Summary: ASCs from obese donors exhibit reduced stem cell characteristics and promote a pro-inflammatory phenotype in macrophage and microglial cells. This highlights the importance of considering individual donor characteristics such as obesity when selecting donors and cells for use in ASC therapeutic applications and regenerative medicine.
Article
Biochemistry & Molecular Biology
Qi Gao, Claire Rhee, Masahiro Maruyama, Zhong Li, Huaishuang Shen, Ning Zhang, Takeshi Utsunomiya, Elijah Ejun Huang, Zhenyu Yao, Bruce A. Bunnell, Hang Lin, Rocky S. Tuan, Stuart B. Goodman
Summary: Wear debris from joint arthroplasties can cause inflammation and implant failure, with macrophages playing a significant role in bone healing and regeneration. M1 macrophages are pro-inflammatory, while M2 macrophages aid in tissue repair. A 3D co-culture system showed that in the presence of inflammation-inducing particles, co-culturing MSCs with M2 macrophages led to increased osteogenic marker expression and enhanced bone formation.
Article
Cell & Tissue Engineering
Fabiana Zanata, Lowry Curley, Elizabeth Martin, Annie Bowles, Bruce A. Bunnell, Xiying Wu, Lydia Masako Ferreira, Jeffrey M. Gimble
Summary: This study found similarities between ASC and DF in terms of surface marker expression, differentiation potential, and gene expression, but ASC may have a relatively stronger adipogenesis. However, there is substantial overlap between ASC and DF phenotypes based on biochemical and differentiation metrics.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Qi Gao, Zhong Li, Claire Rhee, Shiqi Xiang, Masahiro Maruyama, Elijah Ejun Huang, Zhenyu Yao, Bruce A. Bunnell, Rocky S. Tuan, Hang Lin, Michael S. Gold, Stuart B. Goodman
Summary: The immune system modulates the function of fibroblasts, and different sources of differentiated fibroblasts may lead to divergent results. M2 macrophages may be a critical target for the clinical treatment of fibrosis induced by polyethylene particles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Fokhrul Hossain, Samarpan Majumder, Justin David, Bruce A. Bunnell, Lucio Miele
Summary: The study investigates how obesity modulates the gut microbiome in a TNBC mouse model, finding that obesity decreases alpha diversity. Obesity was the only significant factor explaining the similarity of bacterial communities. In functional profile analysis, obesity, tumor presence, and obesity-tumor interaction were significant in explaining variation, with obesity having the strongest correlation.
Editorial Material
Cell Biology
Bruce A. Bunnell
Summary: This article discusses the importance and potential clinical applications of stem cells within adipose tissue, which are multipotent and secrete various cytokines.
Article
Cell & Tissue Engineering
Katie M. Hamel, Kara Q. Liimatta, Jorge A. Belgodere, Bruce A. Bunnell, Jeffrey M. Gimble, Elizabeth C. Martin
Summary: This review article provides important insights into the role of adipose stromal/stem cells (ASCs) in tumor and wound healing, as well as the impact of donor age on ASC behavior in these processes.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Cell Biology
Rachel M. Wise, Sara Al-Ghadban, Mark A. A. Harrison, Brianne N. Sullivan, Emily R. Monaco, Sarah J. Aleman, Umberto M. Donato, Bruce A. Bunnell
Summary: Preconditioning with rapamycin can enhance the therapeutic potential of human adipose-derived stem cells (hASCs) by improving their immunomodulatory properties. This strategy may be effective in treating inflammation-driven pathological conditions.
Article
Endocrinology & Metabolism
Fahima Munmun, Omair A. Mohiuddin, Van T. Hoang, Matthew E. Burow, Bruce A. Bunnell, Veronica M. Sola, Agata R. Carpentieri, Paula A. Witt-Enderby
Summary: This study demonstrates for the first time the role of MEK1/2 and MEK5 in modulating melatonin-mediated actions on bone formation in vivo and in a sex-specific manner.
JOURNAL OF PINEAL RESEARCH
(2022)
Article
Cell Biology
Benjamen T. O'Donnell, Tia A. Monjure, Sara Al-Ghadban, Clara J. Ives, Michael P. L'Ecuyer, Claire Rhee, Monica Romero-Lopez, Zhong Li, Stuart B. Goodman, Hang Lin, Rocky S. Tuan, Bruce A. Bunnell
Summary: This study explores the link between pre-diabetes and osteoarthritis, showing that pre-diabetic conditions can affect the characteristics of adipose stem cells in the infrapatellar fat pad, including their inflammatory gene expression and lipid hormone secretion. The findings suggest that the heightened systemic inflammation associated with untreated T2D may prime the adipose stem cells to exhibit enhanced anti-inflammatory characteristics, potentially influencing the onset and progression of osteoarthritis.
Review
Oncology
Bruce A. Bunnell, Elizabeth C. Martin, Margarite D. Matossian, Courtney K. Brock, Khoa Nguyen, Bridgette Collins-Burow, Matthew E. Burow
Summary: The increase in obesity has led to more adipose stromal/stem cells (ASCs) in the body, which have been shown to impact cancer progression. Obesity-induced alterations in the biological properties of ASCs contribute to enhanced tumorigenesis and metastasis of cancer cells. The interaction between obesity and ASCs plays a role in the development and aggressiveness of various cancers.
CANCER AND METASTASIS REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Zhong Alan Li, Shilpa Sant, Sung Kwon Cho, Stuart B. Goodman, Bruce A. Bunnell, Rocky S. Tuan, Michael S. Gold, Hang Lin
Summary: Synovial joint disorders like osteoarthritis and rheumatoid arthritis are common worldwide, but current clinical management does not fully restore joint function. Organ-on-chip (OoC) technology, which can culture multiple human cell-derived tissues/organs, shows potential in developing better therapies for joint diseases.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Meagan J. Makarczyk, Sophie Hines, Haruyo Yagi, Zhong Alan Li, Alyssa M. Aguglia, Justin Zbikowski, Anne-Marie Padget, Qi Gao, Bruce A. Bunnell, Stuart B. Goodman, Hang Lin
Summary: Osteoarthritis (OA) is a common joint disease that causes pain and disability. The miniJoint model, which mimics the nature of OA in humans, was used to explore the potential of co-treatment with anti-inflammation and chondroinducing agents. The study demonstrated that the combined treatment with BMP-7 and NF-kappa B ODNs reduced inflammation and promoted glycosaminoglycan formation in cartilage, indicating the potential for developing disease-modifying OA drugs.
Article
Genetics & Heredity
Widler Casy, Irvin T. Garza, Xin Chen, Thomas Dong, Yuhui Hu, Mohammed Kanchwala, Cynthia B. Trygg, Charles Shyng, Chao Xing, Bruce A. Bunnell, Stephen E. Braun, Steven J. Gray
Summary: The use of AAV capsid libraries with selection strategies has successfully generated novel AAVs with enhanced features. However, the inability to sequence the complete capsid gene in a high-throughput manner has limited capsid engineering. To overcome this limitation, AAV capsid shuffled libraries were generated and directed evolution was applied in mice and non-human primates to yield AAVs compatible for translational applications. The use of single molecule real-time sequencing and nuclei-enrichment step provided insights for variant identification in the central nervous system.