Article
Oncology
Daejin Lim, Kwangsoo Kim, Taner Duysak, EunA. So, Jae -Ho Jeong, Hyon E. Choy
Summary: A novel anti-cancer therapy based on avian-host-specific serotype Salmonella enterica serovar Gallinarum deficient in ppGpp synthesis is reported. The study showed that this strain specifically targeted and accumulated in tumor tissues, leading to a strong anti-cancer effect in mouse models.
MOLECULAR THERAPY ONCOLYTICS
(2023)
Article
Microbiology
Yan Li, Pei Zhang, Pengcheng Du, Yujiao Mu, Shenghui Cui, Seamus Fanning, Zhiqiang Wang, Ruichao Li, Li Bai
Summary: Hybrid plasmids formed by fusion events in Salmonella enterica serovar 4,[5],12:i:- possess high plasticity and can recombine with other plasmids to form fusion plasmids of different sizes. The structural polymorphisms of plasmids are mainly mediated by insertion sequences and can rearrange the internal structures of the plasmids. This study provides insights into the transmission risk and evolution of multidrug resistance plasmids.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Microbiology
Lester Hiley, Rikki M. A. Graham, Amy V. Jennison
Summary: This study identified two genes possibly associated with change of phage type in IncI1 plasmids. IncI1 plasmids were classified as either 'Delta' or 'Col' plasmids, and genetic recombination leading to a switch between Delta and Col gene sets was observed at the rfsF site. Widespread distribution of these plasmids with extensive regions showing high sequence identity was also indicated.
Article
Infectious Diseases
Meiling Liu, Kunpeng Zhu, Xinge Li, Yiran Han, Chaojie Yang, Hongbo Liu, Xinyin Du, Xuebin Xu, Haiyan Yang, Hongbin Song, Shaofu Qiu, Ying Xiang
Summary: This study investigated the resistance mechanism of a Salmonella Typhimurium strain isolated from an infant's faecal sample, which exhibited resistance to multiple antibiotics. Through whole genome sequencing, multilocus sequence typing, and plasmid analysis, it was found that the strain carried multiple antimicrobial resistance genes and plasmids.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Yinxia Li, Yaowen Zhang, Maoyi Chen, Jie Hu, Haoran Zhang, Ying Xiang, Haiyan Yang, Shaofu Qiu, Hongbin Song
Summary: The study identified a multidrug-resistant Salmonella strain carrying the mcr-1 gene in China, showing resistance to multiple antibiotics. The plasmid pJZ26 could be transferred to E. coli and harbored other resistance genes, posing a challenge to clinical treatment.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Microbiology
Seul Kim, Eunsuk Kim, Hyunjin Yoon
Summary: Salmonella alters cellular processes to improve intracellular fitness during host infection by rewiring cellular transcriptional regulation through alternative sigma factors such as rpoS. Deprivation of outer membrane protein YcfR in Salmonella Typhimurium leads to decreased intracellular survival and downregulation of SPI-2 genes, which is abolished in the absence of rpoS. RpoS-associated stress responses in Salmonella due to impaired envelope integrity may reciprocally downregulate the expression of SPI-2 genes to reduce its virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Fabio Fiorino, Elena Pettini, Oliver Koeberling, Annalisa Ciabattini, Gianni Pozzi, Laura B. Martin, Donata Medaglini
Summary: STm vaccine based on GMMA technology can induce long-lasting anti-bacterial immunity in mice and have a preventive effect on iNTS disease.
Article
Microbiology
Na Sun, Yanying Song, Cong Liu, Mengda Liu, Lanping Yu, Fangkun Wang
Summary: This study aimed to clarify the mechanism of Salmonella survival and reproduction in the host by analyzing differentially expressed genes through high-throughput sequencing. A total of 1340 significant DEGs were screened, with functional annotation revealing their roles in regulation, metabolism, transport, binding, pathogenesis, and motility. Among the upregulated DEGs, 26 were identified as candidate key genes for survival and proliferation in vivo.
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Manal Mohammed, Beata Orzechowska
Summary: The study aimed to characterize phage resistance mechanisms and genomic differences in S. Typhimurium DT104 and DT104b that may explain divergent phage reaction patterns, but found no unique genetic determinants. Experimental assessment of phage-specific receptors on the bacterial cell surface and analysis of bacterial transcriptome using RNA sequencing were suggested to further understand bacterial susceptibility to phages. The use of Anderson phage typing scheme of Salmonella Typhimurium can help improve the understanding of host-phage interactions and lead to the development of phage-based technologies for infection control.
Article
Microbiology
Jin-Fei Zhang, Liang-Xing Fang, Man-Xia Chang, Ming Cheng, Hui Zhang, Teng-Fei Long, Qian Li, Xin-Lei Lian, Jian Sun, Xiao-Ping Liao, Ya-Hong Liu
Summary: Understanding the fitness costs associated with plasmid carriage is crucial for understanding the mechanisms of plasmid maintenance in bacteria. This study investigated the compensatory mechanisms used by Salmonella enterica serovar Typhimurium to maintain a multidrug-resistant plasmid in the presence and absence of antibiotics. It was found that specific plasmid genes and the MDR regions played a role in the fitness costs, and plasmid deletions could compensate for these costs. The study also found that chromosomal gene mutations and altered mRNA expression correlated with changes in bacterial physiology. These findings contribute to our understanding of plasmid maintenance and antibiotic resistance evolution.
Article
Immunology
Shamima Nasrin, Fabien J. Fuche, Khandra T. Sears, Jennifer A. Jones, Myron M. Levine, Raphael Simon, Sharon M. Tennant
Summary: Non-typhoidal Salmonella is a major cause of gastroenteritis, but research on vaccines is limited. Researchers developed a live attenuated O:8 vaccine with good tolerability and immune response against COPS, showing protection in a mouse model.
Article
Microbiology
Somaieh Sabzali, Majid Bouzari
Summary: Salmonellosis is a significant global foodborne disease, with multidrug-resistant Salmonella spp. becoming a worldwide problem. This study isolated and characterized bacteriophages specific to Salmonella Paratyphi B and S. Typhimurium, with a focus on antibiotic resistance and ESBL gene prevalence. The isolated phages show potential for controlling food contamination and phage therapy for infected animals.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Multidisciplinary Sciences
Danielle J. Ingle, Rebecca L. Ambrose, Sarah L. Baines, Sebastian Duchene, Anders Goncalves da Silva, Darren Y. J. Lee, Miriam Jones, Mary Valcanis, George Taiaroa, Susan A. Ballard, Martyn D. Kirk, Benjamin P. Howden, Jaclyn S. Pearson, Deborah A. Williamson
Summary: Three distinct lineages of Salmonella 4,[5],12:i:- have emerged in Australia, likely reflecting differential antimicrobial selection pressures, with two lineages associated with travel to South-East Asia and the third lineage endemic to Australia. These antimicrobial-resistant lineages efficiently infect and survive in host cells, suggesting a suppression of host immune response facilitating their persistence.
NATURE COMMUNICATIONS
(2021)