Article
Multidisciplinary Sciences
Yulian Tang, Anni Ni, Shu Li, Lishuang Sun, Genliang Li
Summary: This study investigated the expression and localization of P4HB in the testis tissues of Chinese mitten crab (Eriocheir sinensis). The results showed that P4HB was expressed in both adult and juvenile E. sinensis testis tissues, with different expression patterns observed in different male germ cells at various developmental stages.
Article
Environmental Sciences
Lan -Min Wang, Ke Jia, Zhen-Fang Li, Hong -Yu Qi, Ding -Xi Liu, Ya-Jing Liang, Shuang-Li Hao, Fu-Qing Tan, Wan-Xi Yang
Summary: Recent studies found that TiO2 nanoparticles (TiO2-NPs) have reproductive toxicity in males, but there are few reports on the toxicity of TiO2-NPs in crustaceans. In this study, freshwater crustacean Eriocheir sinensis was used to investigate the male toxicity of TiO2-NP exposure and its underlying mechanisms. TiO2-NPs of 3 nm and 25 nm at a dose of 30 mg/kg bw induced apoptosis and damage to the haemolymph-testis-barrier (HTB) and seminiferous tubule structure. The mTORC1-mTORC2 imbalance induced by TiO2-NPs was involved in the mechanism of AJ and HTB disruption, resulting in spermatogenesis in E. sinensis.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Ding -Xi Liu, Zhen-Fang Li, Yan-Shuang Zhao, Lan-Min Wang, Hong -Yu Qi, Zhan Zhao, Fu-Qing Tan, Wan-Xi Yang
Summary: The study found that Es-beta-CATENIN participates in maintaining the hemolymph-testes barrier in the spermatogenesis of E. sinensis. By interacting with other proteins, it maintains the integrity of the hemolymph-testes barrier and also regulates cell proliferation and apoptosis, playing a crucial role in normal sperm release.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Hong-Yu Qi, Zhen-Fang Li, Lan-Min Wang, Zhan Zhao, Jia-Ming Wang, Fu-Qing Tan, Wan-Xi Yang
Summary: In this study, we identified the macromolecule es-Myosin VI in Eriocheir sinensis and found its co-localization with F-actin in the testis. Knockdown of es-Myosin VI led to a decrease in spermatozoa count, disruption of intercellular junctions, and damage to the hemolymph-testis barrier. We also observed changes in the localization of es-LHR and consistent results in the MAPK signalling pathway and spermatogenesis defects between the es-LHR and es-Myosin VI knockdown groups. These findings suggest that es-Myosin VI plays an important role in male reproduction in Crustacea.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Developmental Biology
Bang-Hong Wei, Jia-Hao Ni, Tong Yang, Shuang-Li Hao, Wan-Xi Yang
Summary: This study identified three Piwi proteins highly expressed in the testis of Eriocheir sinensis, playing roles in nucleus formation and apoptosis pathways in germ cells to maintain germ cell integrity. Knockdown of PIWIs resulted in abnormal nucleus formation and inhibition of apoptosis, leading to an increase in abnormal spermatozoa.
Article
Fisheries
Lvjing Luo, Yulian Tang, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Genliang Li
Summary: The aim of this study was to investigate the regulatory role of miR-133b on the expression of SAP30 during sperm nuclear decondensation. Using various techniques, the expressions of SAP30 at mRNA and protein levels and miR-133b targeting SAP30 were analyzed in the testis tissues of adult and juvenile Eriocheir sinensis. The results showed that SAP30 was differentially expressed in decondensed nuclei of E. sinensis testis tissue and miR-133b targeting SAP30 was also differentially expressed in adult and juvenile crab testis tissues, indicating a potential regulatory relationship between SAP30 and miR-133b.
AQUACULTURE REPORTS
(2023)
Article
Anatomy & Morphology
Jia-Ming Wang, Zhen-Fang Li, Hong-Yu Qi, Zhan Zhao, Wan-Xi Yang
Summary: The actin binding proteins es-Arp3 and es-Eps8 are found to be crucial for cell adhesion and spermatogenesis in the Chinese mitten crab. Their deficiency results in delayed spermatozoa release, shedding of germ cells, disorganized actin filaments, and abnormal expression of junctional proteins.
Article
Genetics & Heredity
Chao Li, Ruifang Yu, Huan Liu, Jiashan Qiao, Fenghao Zhang, Shumei Mu, Mingshen Guo, Han Zhang, Yanqin Li, Xianjiang Kang
Summary: This study investigates the function of mitochondrial proteins in acrosome formation in Eriocheir sinensis sperm. By comparing different inducers and observing morphological changes, it explores the role of mitochondrial proteins in acrosome formation. LC-MS/MS analysis reveals a total of 945 proteins released during the acrosome reaction, with 17.57% derived from mitochondria. Immunofluorescence analysis confirms the independent involvement of malate dehydrogenase and voltage-dependent anion channel 3 in acrosomal membrane formation.
Article
Fisheries
Lishuang Sun, Anni Ni, Yulian Tang, Shu Li, Genliang Li
Summary: The protein Add1 is involved in the decondensation of sperm nuclei and the maintenance and protection of non-condensed chromatin during spermiogenesis of the Chinese mitten crab, Eriocheir sinensis.
AQUACULTURE INTERNATIONAL
(2022)
Article
Cell Biology
Zhen-Fang Li, Hong-Yu Qi, Jia-Ming Wang, Zhan Zhao, Fu-Qing Tan, Wan-Xi Yang
Summary: This study cloned mTORC1/rpS6 and mTORC2/PKC proteins from the testis of Eriocheir sinensis for the first time and found that they regulate spermatogenesis in crustaceans through Arp3-mediated actin microfilament organization. These findings reveal the importance of the mTOR signaling pathway in spermatogenesis in arthropods.
CELL AND TISSUE RESEARCH
(2023)
Article
Cell Biology
Zhen-Fang Li, Shuang-Li Hao, Lan-Min Wang, Hong-Yu Qi, Jia-Ming Wang, Fu-Qing Tan, Wan-Xi Yang
Summary: This study identified two core components of the mTOR signaling pathway, es-Raptor and es-Rictor, in spermatogenesis of the Chinese mitten crab. These proteins are crucial for maintaining the actin filament network and cell junctions, which are essential for successful sperm production.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chao Li, Jiao Chen, Shumei Mu, Han Zhang, Mingshen Guo, Yanqin Li, Zhaohui Zhang, Xianjiang Kang
Summary: This study identified seven Rab proteins in the sperm proteome of Eriocheir sinensis and confirmed the involvement of Rab2 and Rab6 in acrosome formation. These findings provide a foundation for understanding the functions of Rab proteins during spermatogenesis in Decapoda animals.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Multidisciplinary Sciences
Nan Yang, Wenjing Li, Wenrong Feng, Meiyao Wang, Aimin Liu, Yongkai Tang, Shengyan Su
Summary: This study focused on the de novo genome and full-length transcriptome analysis of Eriocheir sinensis, a well-known species of hairy crab found in Yangcheng Lake, to gain a deeper understanding of the genetic factors influencing its growth and development. The findings contribute to indirect comprehension of the genetic variations underlying individual traits and establish a basis for further investigations on the Chinese mitten crab.
Article
Food Science & Technology
Long Zhang, Ning-Ping Tao, Xugan Wu, Xichang Wang
Summary: Chinese mitten crab, as an important seafood in China, is affected by the color and bitterness of its hepatopancreas, which can significantly impact consumer acceptance. This study investigated the metabolic relationship between key metabolites and the color and bitterness in the hepatopancreas of the crab. The results suggest that bile acids and bitter free amino acids may contribute to the bitterness of the hepatopancreas.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biology
Ke Jia, Fu-Qing Tan, Shuang-Li Hao, Wan-Xi Yang
Summary: This study revealed the crucial role of Es-SOX8 protein in the spermatogenesis of E. sinensis, and showed that it can directly regulate the transcription of Es-BMP2, thus affecting the sperm nucleus deformation process.
Article
Fisheries
Muxue Tang, Xuejie Li, Lei Yang, Qun Wang, Weiwei Li
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2020)
Article
Gastroenterology & Hepatology
Dan Li, Nan Li, Yi-Fan Zhang, Haiying Fu, Mingqian Feng, Dina Schneider, Ling Su, Xiaolin Wu, Jing Zhou, Sean Mackay, Josh Kramer, Zhijian Duan, Hongjia Yang, Aarti Kolluri, Alissa M. Hummer, Madeline B. Torres, Hu Zhu, Matthew D. Hall, Xiaoling Luo, Jinqiu Chen, Qun Wang, Daniel Abate-Daga, Boro Dropublic, Stephen M. Hewitt, Rimas J. Orentas, Tim F. Greten, Mitchell Ho
Review
Fisheries
Weiwei Li, Qun Wang
Summary: Crustaceans, such as crabs and shrimp, lack adaptive immunity and rely solely on innate immunity for pathogen defense. The white spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimps and is a widely prevalent viral disease. Crabs serve as interesting models for studying immune responses to WSSV infection, with recent reviews focusing on antiviral functions such as exosome-mediated apoptosis and alternatively spliced Down syndrome cell adhesion molecule.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yue-Hong Zhao, Hao Li, Hui Zhao, Wei-Kang Sun, Qun Wang, Wei-Wei Li
Summary: Interleukins (ILs) are cytokines crucial for immunity, typically found only in vertebrates. However, a IL-16-like gene (EsIL-16) was identified in the Chinese mitten crab, which promotes cell proliferation and interacts with integrin beta 1 to protect against bacterial infection.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Fisheries
Yu Song, Kaimin Zhou, Xingyu Nan, Yukai Qin, Ke Zhao, Weiwei Li, Qun Wang
Summary: The study found that ML proteins were involved in bacterial resistance in Chinese mitten crab, with one member, EsML3, being upregulated in hemocytes following bacterial challenge and binding to Gram-negative and Gram-positive bacteria. EsML3 mediates cellular immune responses by promoting bacterial clearance and phagocytosis against bacterial infection in crab.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Hao Li, Xing-Kun Jin, Kai-Min Zhou, Hui Zhao, Yue-Hong Zhao, Qun Wang, Wei-Wei Li
Summary: A novel Dscam signaling pathway was investigated in crab, revealing its crucial functions during immune responses in invertebrates. Bacterial infection induced the cleavage of Dscam ICDs and facilitated nuclear translocation, promoting hemocyte proliferation and host protection. ADAM10 was found to play a key role in Dscam shedding activity post bacterial stimulation, mediating the cleavage of ICDs by gamma-secretase.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Fisheries
Hao Li, Yuehong Zhao, Xiaoli Zhang, Hui Zhao, Weiwei Li, Qun Wang
Summary: In this study, the transcriptional response of Eriocheir sinensis to different forms of Dscam ICDs was investigated. The results showed that different forms of Dscam ICDs can affect key immune, metabolic, and cell proliferation-regulated genes and pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Kaimin Zhou, Longwei Bai, Xingyu Nan, Ke Zhao, Yu Song, Weiwei Li, Qun Wang
Summary: In this study, the essential role of FADD in the IMD signaling pathway in the Chinese mitten crab Eriocheir sinensis was demonstrated. Knockdown of EsFADD led to impaired immune response and increased susceptibility to bacterial infection. The findings highlight the evolutionary conservation of FADD in crustaceans and provide insights into innate immune defense in invertebrates.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Jiying Li, Ke Zhao, Hao Li, Kaimin Zhou, Qun Wang, Weiwei Li
Summary: The study revealed that the Dscam gene in the Chinese mitten crab has different variants that play a role in immune regulation, with significant increase in expression levels after bacterial stimulation. The two variants also differ in their regulation of antimicrobial peptides, proportion of carried exons, and bacterial phagocytosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhao, Zhe Chen, Hao Li, Yue-Hong Zhao, Qun Wang, Wei-Wei Li
Summary: This study demonstrates that bacteria can induce hemocyte proliferation in Chinese mitten crabs, and EsCsn5 plays a negative regulatory role in this process by controlling the degradation of EsCyclin E. Knockdown of EsCsn5 enhances hemocyte proliferation and reduces survival rate in crabs, leading to increased bacterial concentration in the hemolymph.
Article
Immunology
Kaimin Zhou, Yukai Qin, Yu Song, Ke Zhao, Weijuan Pan, Xingyu Nan, Yue Wang, Qun Wang, Weiwei Li
Summary: The study reveals the antibacterial role of a member of the C-type lectin family, EsIgLectin, in the Chinese mitten crab. EsIgLectin is expressed in hemocytes and intestines, and it plays a critical role in bacterial binding, agglutination, growth inhibition, and clearance. It promotes bacterial phagocytosis in hemocytes and protects against inflammation in the intestines, functioning in a polymeric Ig receptor-dependent manner.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Fisheries
Yue Wang, Xingyu Nan, Shuqi Hao, Ke Zhao, Yanan Guo, Qun Wang, Weiwei Li
Summary: AKT plays an important role in regulating cell metabolism and cell proliferation in Eriocheir sinensis. The EsAkt protein is highly conserved across species, and its expression is up-regulated in hemocytes after stimulation with gram-positive or gram-negative bacteria. Knockout of the EsAkt gene reduces cell glucose metabolism and inhibits cell proliferation.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Weikang Sun, Hao Li, Yuehong Zhao, Longwei Bai, Yukai Qin, Qun Wang, Weiwei Li
Summary: The study identified three conserved VG domains in the Chinese mitten crab, highlighting functional similarities of Vg in vertebrates and invertebrates. Among these domains, DUF1943 and VWD showed bacterial binding activity, with only the VWD domain inhibiting bacterial proliferation. Furthermore, the study demonstrated that EsVg regulates hemocyte phagocytosis by binding with EspIgR through the DUF1943 domain, promoting bacterial clearance and protecting the host from infection.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Fisheries
Longwei Bai, Kaimin Zhou, Hao Li, Yukai Qin, Qun Wang, Weiwei Li
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Zoology
Qing Li, Haitao Zhao, Lin He, Hongdan Yang, Qun Wang
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.
