Journal
GENE
Volume 516, Issue 1, Pages 184-189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2012.11.052
Keywords
Chlorella species; Temperature stress; HSP70B; Clonal assay; Western blotting
Categories
Funding
- Bulgarian Ministry of Education, Young and Science [D-002-317]
- RAS
- BAS
- WETLANET, FP7 CSA - SUPPORT ACTION [229802]
- Program MCB RAS
Ask authors/readers for more resources
The cellular response of three Chlorella species that differ in their temperature preferences and tolerance - Chlorella vulgaris Antarctic, C vulgaris strain 8/1 - thermophilic, and Chlorella kesslery - mesophilic - is analysed. Interspecies variability is found for the sensitivity of the species, with respect to the constitutive and induced levels of HSP70B. Based on their sensitivity to heat, the species were ranked as follows: C vulgaris > C vulgaris 8/1>C kesslery. A higher constitutive level and the well-expressed overproduction of HSP70B estimated for C. vulgaris are most likely among the factors that allow it to survive in the extreme Antarctic environment. The overexpression of HSP70B could be characterised as a short-lived stress response as the most pronounced enhancement is measured at 2 h after the stress. We also show that HSP70B may be used as a marker of thermal stress in these organisms. Our results contribute to the hypothesis of the conserved functional properties of HSP70B as a mechanism of thermotolerance in plants. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available