Article
Cell Biology
Yajie Wang, Zhengyang Li, Shizan Xu, Wenjun Li, Mengyun Chen, Miao Jiang, Xiaoming Fan
Summary: FIRRE is overexpressed in colorectal cancer and is associated with cell migration, invasion, proliferation, lymphatic metastasis, and distant metastasis. FIRRE physically interacts with PTBP1, leading to the translocation of PTBP1 from nucleus to cytoplasm and stabilization of BECN1 mRNA. FIRRE also contributes to autophagy activity.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Qiao-Xia Zhang, Yu-Ming Pan, Hong-Li Xiao, Na An, Si -Si Deng, Xin DU
Summary: Alternative splicing is a crucial post-transcriptional regulation process in tumors, and PTBP1 might be a potential target for AML therapy.
Review
Pharmacology & Pharmacy
Jianguo Feng, Jianlong Zhou, Yunxiao Lin, Wenhua Huang
Summary: This article highlights the importance of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) in regulating RNA metabolism and its dysregulation in various diseases. The selective binding sites of hnRNP A1 to RNA and DNA are summarized, along with the co-regulatory factors that interact with hnRNP A1. The potential therapeutic implications of targeting hnRNP A1 are discussed, including small-molecule drugs and biomedicines.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Infectious Diseases
Vinithra Iyer, Sheetal Tushir, Shreekant Verma, Sudeshna Majumdar, Srimonta Gayen, Rakesh Mishra, Utpal Tatu
Summary: The study reveals the split nature of the Hsp90 gene in G. lamblia, with two ORFs separated on chromosome 5 and connected through a unique trans-splicing reaction. The presence of canonical nucleotide elements in independent pre-mRNAs suggests the involvement of spliceosomes in trans-splicing of Hsp90. Research also shows that an RNA helicase interacts with HspN pre-mRNA, and the trans-spliced genes of Giardia are spatially close in the nucleus.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Immunology
Allison R. Wagner, Haley M. Scott, Kelsi O. West, Krystal J. Vail, Timothy C. Fitzsimons, Aja K. Coleman, Kaitlyn E. Carter, Robert O. Watson, Kristin L. Patrick
Summary: The study reveals that SR/hnRNPs proteins play a crucial role in regulating macrophage gene expression and alternative splicing, with different functions in infected and uninfected cells. These proteins influence thousands of macrophage transcripts in differential isoform usage, impacting immune response and susceptibility to pathogens. The findings suggest a model where pre-mRNA splicing is critical for controlling innate immune gene expression and infection outcomes in macrophages ex vivo.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Geriatrics & Gerontology
Yiying Zhou, Ke Zhang, Fangmin Wang, Jiali Chen, Shanshan Chen, Manqing Wu, Miaojun Lai, Yisheng Zhang, Wenhua Zhou
Summary: This study investigates the regenerative mechanisms of knockdown the RNA-binding protein polypyrimidine tract binding protein (PTB) in reversing depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons. The findings suggest that supplementing cholinergic neurons after PTB knockdown may be a promising therapeutic strategy to revert depression-like behaviors and cognitive impairment.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Review
Cell Biology
Shirui Dai, Chao Wang, Cheng Zhang, Lemeng Feng, Wulong Zhang, Xuezhi Zhou, Ye He, Xiaobo Xia, Baihua Chen, Weitao Song
Summary: Polypyrimidine tract-binding protein (PTB) functions by shuttling between the nucleus and the cytoplasm, and is involved in RNA splicing and metabolism, with significant implications for physiological and pathological processes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ping Chang, Hsin-Yu Hsieh, Shih-Long Tu
Summary: In this study, the splicing factor RBP45d was identified in Arabidopsis, functioning with PRP39a in temperature-mediated flowering. RBP45d participates in initial pre-mRNA splicing and modulates temperature-sensitive alternative splicing of FLOWERING LOCUS M to control flowering time in Arabidopsis.
Article
Chemistry, Physical
Jussara Amato, Nunzia Iaccarino, Federica D'Aria, Francesco D'Amico, Antonio Randazzo, Concetta Giancola, Attilio Cesaro, Silvia Di Fonzo, Bruno Pagano
Summary: This study investigates the conformational equilibria of sequences forming noncanonical DNA structures in the promoter regions of important oncogenes BCL2 and KRAS, and their dependence on pH and cell-mimicking molecular crowding conditions. The results reveal that the i-motif structures in the promoters of BCL2 and KRAS are highly dynamic and exist in pH-dependent equilibrium with hairpin and hybrid i-motif/hairpin species. The study provides valuable insights into the factors that influence the molecular-level equilibrium between different conformational species involved in oncogene expression.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Plant Sciences
Shuai Lu, Cong Gao, Yongzhou Wang, Yingying He, Junrong Du, Moxian Chen, Hua Zhao, Hui Fang, Baohua Wang, Yunying Cao
Summary: This study identified U2AF65A in plant species, conducted phylogenetic and bioinformatic analyses, and studied the localization and stress responses of OsU2AF65A in rice. Using Arabidopsis and rice mutants, the importance of U2AF65A in plant growth and stress responses was demonstrated. These findings provide an overview of the U2AF65 gene family and its role in plant development and stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Jana Koch, Sebastian J. Schober, Sruthi Hindupur, Caroline Schoening, Florian G. Klein, Klaus Mantwill, Maximilian Ehrenfeld, Ulrike Schillinger, Timmy Hohnecker, Pan Qi, Katja Steiger, Michaela Aichler, Jurgen E. Gschwend, Roman Nawroth, Per Sonne Holm
Summary: The combination of CDK4/6 inhibitors and oncolytic viruses enhances their anti-tumor effect by depleting Retinoblastoma protein levels, promoting virus replication and increasing the production of oncolytic viruses in cancer cells, leading to a more efficient tumor treatment.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Naoko Kajitani, Stefan Schwartz
Summary: This review highlights the importance of RNA-binding proteins in controlling HPV gene expression, particularly in regulating the oncogenic E6 and E7 genes and immunogenic L1 and L2 proteins, which play a role in immune evasion during carcinogenesis.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Zhen Li, Zekang Ye, Jiazheng Ma, Qian Gu, Jianzhen Teng, Xiaoxuan Gong
Summary: The study revealed that miR-133b alleviated doxorubicin-induced cardiomyocyte apoptosis and cardiac fibrosis by targeting PTBP1 and TAGLN2, suggesting that miR-133b may be a potential biomarker for doxorubicin-induced cardiac injury.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Eliezra Glasser, Debanjana Maji, Giulia Biancon, Anees Mohammed Keedakkatt Puthenpeedikakkal, Chapin E. Cavender, Toma Tebaldi, Jermaine L. Jenkins, David H. Mathews, Stephanie Halene, Clara L. Kielkopf
Summary: This study reveals the flexibility of U2AF2 in recognizing polypyrimidine tracts of nascent transcripts and the tolerance of nucleotide substitutions at the central position of the Py tract. The results highlight the importance of RNA flexibility for protein recognition and provide insights into the relationship between splice site motifs and pre-mRNA splicing efficiencies.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kaushik Saha, Gourisankar Ghosh
Summary: This study reveals a novel function of U1 snRNP in defining splicing substrates in mammals. It demonstrates how SR proteins and U1 snRNP cooperate to generate a substrate for spliceosome assembly. The excess U1 snRNP selectively displaces some SR proteins, allowing for sequential recognition of splice signals. This mechanism may be employed by other splicing regulatory proteins.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Federica Vurro, Ylenia Jabalera, Silvia Mannucci, Giulia Glorani, Alberto Sola-Leyva, Marco Gerosa, Alessandro Romeo, Maria Grazia Romanelli, Manuela Malatesta, Laura Calderan, Guillermo R. Iglesias, Maria P. Carrasco-Jimenez, Concepcion Jimenez-Lopez, Massimiliano Perduca
Summary: MamC-expressed BMNPs have advantages in stable functionalization and molecule release, but face challenges in cell internalization. Encapsulation with PLGA and functionalization with TAT peptide enhance internalization without compromising cell viability.
Review
Biochemistry & Molecular Biology
Marilisa Galasso, Simona Gambino, Maria Grazia Romanelli, Massimo Donadelli, Maria Teresa Scupoli
Summary: This study discusses the role of catalase in cancer, emphasizing its dichotomous role in cancer and providing a comprehensive description of the regulatory mechanisms associated with catalase at multiple levels.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Medicine, General & Internal
Maurizio Di Marco, Francesca Urbano, Agnese Filippello, Stefania Di Mauro, Alessandra Scamporrino, Nicoletta Miano, Giuseppe Coppolino, Giuseppe L'Episcopo, Stefano Leggio, Roberto Scicali, Salvatore Piro, Francesco Purrello, Antonino Di Pino
Summary: This study found that subjects with prediabetes exhibit increased platelet reactivity and a proinflammatory profile, which are associated with early markers of cardiovascular disease.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Stefania Di Mauro, Alessandra Scamporrino, Agnese Filippello, Maurizio Di Marco, Maria Teresa Di Martino, Francesca Scionti, Antonino Di Pino, Roberto Scicali, Roberta Malaguarnera, Francesco Purrello, Salvatore Piro
Summary: This study identified a specific molecular signature of diabetic kidney disease (DKD) by analyzing the transcriptome of serum samples from diabetic patients. Downregulation of certain transcripts, including mitochondrial RNAs, was found to be associated with DKD and correlated with markers of kidney function and other clinical parameters. These mitochondrial RNAs showed high diagnostic performance in distinguishing between different stages of DKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Stefania Di Mauro, Agnese Filippello, Alessandra Scamporrino, Francesco Purrello, Salvatore Piro, Roberta Malaguarnera
Summary: Metformin is a commonly prescribed medication for diabetes treatment, but there are concerns about the risk of lactic acidosis due to its similarity to other drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Pilotti, Attilio Cannata, Giacomo Magnani, Fabio Bignami, Andrea Corsi, Maria Teresa Valenti, Mariam Shallak, Greta Forlani, Maria Grazia Romanelli
Summary: This study identified a signature of eight miRNAs associated with HTLV-2 infection and found that altered miRNA expression patterns were correlated with impaired Th cell differentiation and cytokine signaling pathways. Additionally, the study showed that the RUNX2 protein was significantly more expressed in the presence of HTLV-2 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Agnese Filippello, Stefania Di Mauro, Alessandra Scamporrino, Sebastiano Alfio Torrisi, Gian Marco Leggio, Antonino Di Pino, Roberto Scicali, Maurizio Di Marco, Roberta Malaguarnera, Francesco Purrello, Salvatore Piro
Summary: The dysfunctions of intestinal cells in obesity and associated diabetes may be related to impaired intestinal cell development. Lipotoxicity affects the differentiation of specific intestinal cell types, including enterocytes, goblet cells, and CCK cells. These findings provide new targets for obesity and diabetes therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chiara Stefani, Antonella Sangalli, Elena Locatelli, Tania Federico, Giovanni Malerba, Maria Grazia Romanelli, Gustavo Adolfo Arganaraz, Bosco Christiano Maciel Da Silva, Alberto Jose Duarte Da Silva, Jorge Casseb, Enrique Roberto Arganaraz, Alessandra Ruggiero, Donato Zipeto
Summary: This study found a correlation between unstable HLA-C variants and rapid progression to AIDS, providing new insights into the impact of host genetic factors on AIDS progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Andrea Corsi, Cristina Bombieri, Maria Teresa Valenti, Maria Grazia Romanelli
Summary: Tau microtubule-associated proteins play important roles in neurons and are associated with several neurodegenerative diseases. Recent studies have focused on understanding the regulation of Tau splicing to develop new treatment approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alessandra Scamporrino, Stefania Di Mauro, Agnese Filippello, Grazia Di Marco, Antonino Di Pino, Roberto Scicali, Maurizio Di Marco, Emanuele Martorana, Roberta Malaguarnera, Francesco Purrello, Salvatore Piro
Summary: Diabetic kidney disease (DKD) is a complication that significantly increases the risk of end-stage kidney disease and cardiovascular events. The identification of novel, highly sensitive, and specific early biomarkers to diagnose DKD and predict kidney function decline is crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Anna Lagni, Virginia Lotti, Erica Diani, Giada Rossini, Ercole Concia, Claudio Sorio, Davide Gibellini
Summary: Research shows that individuals with cystic fibrosis (CF) have mild symptoms when infected with SARS-CoV-2, suggesting that CFTR expression and function may be involved in the replication of the virus. In laboratory experiments, CFTR inhibitors effectively inhibited the replication of SARS-CoV-2, indicating the importance of CFTR in the virus replication process and providing new insights for further research and potential treatments.
Review
Biochemistry & Molecular Biology
Sabrina Scilletta, Maurizio Di Marco, Nicoletta Miano, Agnese Filippello, Stefania Di Mauro, Alessandra Scamporrino, Marco Musmeci, Giuseppe Coppolino, Francesco Di Giacomo Barbagallo, Giosiana Bosco, Roberto Scicali, Salvatore Piro, Francesco Purrello, Antonino Di Pino
Summary: The classic progression of diabetic kidney disease (DKD) involves glomerular hyperfiltration, microalbuminuria, proteinuria, and decline in estimated glomerular filtration rate (eGFR) leading to dialysis. Recent evidence challenges this concept, suggesting that eGFR decline may occur independently from the development of albuminuria. The identification of a new DKD phenotype, non-albuminuric DKD, has raised questions about its pathogenesis and the association between different phenotypes and cardiovascular risk. There is a lack of studies analyzing the effects of drugs on different DKD phenotypes, leading to a lack of specific guidelines for therapy.
Review
Cell Biology
Luca Dalle Carbonare, Arianna Minoia, Sharazed Zouari, Francesca Cristiana Piritore, Anna Vareschi, Maria Grazia Romanelli, Maria Teresa Valenti
Summary: Bone-muscle crosstalk is vital for maintaining the homeostasis of skeletal and muscle tissue, and is facilitated by different molecular signals including osteokines and myokines. The recent discovery of non-coding RNAs has further emphasized their role in promoting crosstalk between bone and muscle. Physical activity not only influences the bone and muscular systems individually, but also enhances their crosstalk by modulating the production of circulating molecular factors. In this review, we will discuss the effects of physical activity on bone and muscle cells, focusing on the biomolecular mechanisms that regulate their cellular interactions.
Review
Microbiology
Erica Diani, Anna Lagni, Virginia Lotti, Emil Tonon, Riccardo Cecchetto, Davide Gibellini
Summary: Flaviviruses cause a wide range of diseases with severe clinical manifestations, resulting in over 400 million infections and an estimated 20,000 deaths worldwide each year. However, there are limited antiviral treatments available, posing a significant public health issue.
Article
Biochemistry & Molecular Biology
Elisa Orlandi, Elisa De Tomi, Rachele Campagnari, Francesca Belpinati, Monica Rodolfo, Elisabetta Vergani, Giovanni Malerba, Macarena Gomez-Lira, Marta Menegazzi, Maria Grazia Romanelli
Summary: This study investigates the influence of sex hormones on the expression of RNASEL and miR-146a genes in melanoma cells. The results suggest that miR-146a overexpression promotes melanoma by suppressing RNase-L protein expression and inducing high levels of ERK1/2. Additionally, testosterone and 17 beta-estradiol have differential effects on the endogenous expression of RNASEL and miR-146a genes.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.
