Journal
GENE
Volume 487, Issue 2, Pages 170-173Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2011.07.005
Keywords
Junmu No. 1 white swine; A-FABP; Intron 1; Polymorphism; Protein expression; Meat quality
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Funding
- Tackle Key Problems in Science and Technology Program from Jilin Province [09ZDGG008]
- Young Foundation of Scientific Research from Agronomy Department of Jilin University [4305050102E2]
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This study was designed to investigate the single nucleotide polymorphism (SNP) in intron 1 of the gene A-FABP in 127 Junmu No. 1 white swine using PCR-SSCP. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism of intron 1 was due to a point mutation in position 3481 bp of A-FABP, giving 3 genotypes (CC, CD and DD). Association analysis indicated that the polymorphism had a significant effect on marbling (P<0.05). Genotype DD had higher marbling than CD and CC, but the difference between CD and CC was no significant. Polymorphism had a highly significant effect on intramuscular fat (IMF) content (P<0.01). DD was higher than CD, which was higher than CC. No significant conclusions can be drawn regarding other traits. Immunoblot analysis of A-FABP levels was carried out on 3 different genotype individuals. Expression was markedly reduced in DD compared with genotype CC. Thus A-FABP may be a candidate gene or a quantitative trait locus-linked gene associated with meat quality traits. (C) 2011 Elsevier B.V. All rights reserved.
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