Article
Multidisciplinary Sciences
Hanqing Tang, Josphat K. Saina, Zhi-Cheng Long, Jinming Chen, Can Dai
Summary: This study assembled transcriptome sequences of Sagittaria trifolia and developed effective EST-SSR markers. A total of 86 microsatellite loci were identified and verified for their stable and polymorphic bands. These markers could be useful for examining population genetic structure, differentiation, and parentage analyses in ecological and evolutionary studies of S. trifolia.
Article
Plant Sciences
Ana Dovile Juskyte, Ingrida Mazeikiene, Vidmantas Stanys
Summary: By analyzing the transcriptome of blackcurrants, this study identified potential R genes associated with resistance to blackcurrant reversion virus. This finding is significant for targeted research and genetic improvement in blackcurrant breeding programs.
Article
Entomology
Haoliang Chen, Minghui Xie, Lulu Lin, Yongzhi Zhong, Feng Zhang, Weihua Su
Summary: Spodoptera frugiperda, an important pest on maize, has spread to many developing countries in Africa and Asia, posing a threat to food security. This study used second-generation sequencing technology to analyze the gene expression changes of S. frugiperda after treatment with three different pesticides, providing insights into pesticide detoxification in this pest.
JOURNAL OF INSECT SCIENCE
(2022)
Article
Agronomy
Tae-Heon Kim, Young-Mi Yoon, Jin-Cheon Park, Jong-Ho Park, Kyong-Ho Kim, Yang-Kil Kim, Jae-Han Son, Tae-Il Park
Summary: This study used transcriptome sequencing to investigate the seedling stage of oat and developed 30 dCAPS markers, which are important for oat genetic analysis, cultivar identification, and breeders' rights protection.
Article
Biotechnology & Applied Microbiology
Li-Min Chen, Xiao-Wei Li, Tian-Jun He, Peng-Ju Li, Yuan Liu, Shu-Xing Zhou, Quan-Cong Wu, Ting-Ting Chen, Yao-Bin Lu, You-Ming Hou
Summary: The study found that tomato significantly reduces concentrations of amino acids, fructose, sucrose, jasmonic acid, salicylic acid, and total phenols compared to eggplant after infestation with Tuta absoluta, with fewer transcript changes at the transcriptional level. Signaling genes, especially those associated with salicylic acid in eggplant, play important roles in mediating plant immunity against Tuta absoluta.
Article
Plant Sciences
Yang Liu, Xiaomei Fang, Tian Tang, Yudong Wang, Yinhuan Wu, Jinyu Luo, Haotian Wu, Yingqian Wang, Jian Zhang, Renwu Ruan, Meiliang Zhou, Kaixuan Zhang, Zelin Yi
Summary: This study sequenced the transcriptome of common buckwheat and Ukrainian daliqiao using high-throughput sequencing technology, obtaining a large amount of gene information and differentially expressed genes. SSR and EST-SSR loci were also detected. The clustering results verified the genetic relationship between common buckwheat and Ukrainian daliqiao. These findings are important for enriching the genetic information and marker-assisted selection breeding of buckwheat.
Article
Agronomy
Hassan S. Al-Zahrani, Tarek A. A. Moussa, Hameed Alsamadany, Rehab M. Hafez, Michael P. Fuller
Summary: This study investigated the gene expression and genetic variation in a super strain of tomatoes (cv. super strain B) cultivated under drought stress in Saudi Arabia. The results showed that specific genes, such as ATP binding genes and protein kinase genes, were widely regulated in response to drought stress. Additionally, several pathways related to metabolism and biosynthesis of secondary metabolites were identified.
Article
Genetics & Heredity
Hanjing Liu, Yuli Zhang, Zhen Wang, Yingjuan Su, Ting Wang
Summary: This study characterized and developed EST-SSRs from transcriptome sequences of Cephalotaxus oliveri, revealing genetic information and potential applications. The identified SSRs were mainly trinucleotide repeats, located in different functional regions, and suitable for genetic diversity analysis. The developed markers lay the foundation for further genetic and adaptive evolution studies in C. oliveri and related species.
FRONTIERS IN GENETICS
(2021)
Article
Genetics & Heredity
Weiwei Luo, Ying Zhou, Junru Wang, Xiaomu Yu, Jingou Tong
Summary: The study utilized Isoform-sequencing technology and RNA-seq data to uncover the transcriptomic landscape of bighead carp in early growth stage, identifying key differentially expressed genes (DEGs) and pathways involved in growth and bone development. This resource could serve as a basis for further investigations into the genetic regulation of growth in fish.
FRONTIERS IN GENETICS
(2021)
Article
Forestry
Ju Wang, Shuai Guo, Yongxin Zhang, Feng Zhang, Yufei Yun, Guosheng Zhang
Summary: This study sequenced the transcriptomes of open-pollinated progeny seedlings of Chinese pine from different provenances and analyzed the expression of genes involved in signaling pathways. It also detected EST-SSR loci and developed polymorphic primers for genetic diversity study and marker-assisted breeding.
Article
Biochemistry & Molecular Biology
Cheng Qin, Mohammad Abass Ahanger, Bo Lin, Ziguang Huang, Jie Zhou, Nadeem Ahmed, Suilong Ai, Nabil Sa Mustafa, Muhammad Ashraf, Lixin Zhang
Summary: The study showed that ACh treatment promoted differential gene expression in Nicotiana benthamiana under salt stress, decreased antioxidant enzyme activity, and increased cell wall biosynthesis and hormone signaling pathway gene expression.
Article
Genetics & Heredity
Niyan Xiang, Bojie Lu, Tao Yuan, Tiange Yang, Jiani Guo, Zhihua Wu, Hong Liu, Xing Liu, Rui Qin
Summary: This study made progress in the molecular genetics and evolution research of the unique Chinese plant Chrysosplenium macrophyllum through transcriptome sequencing and EST-SSR markers, and found a high level of genetic diversity in C. macrophyllum. These developed molecular markers will be significant for the study of genetic diversity and evolutionary history of C. macrophyllum and other Chrysosplenium species.
Article
Multidisciplinary Sciences
Venugopal Vidya, Duraisamy Prasath, Mohandas Snigdha, Ramasamy Gobu, Charles Sona, Chandan Suravi Maiti
Summary: This study identified and validated expressed sequence tag (EST)-simple sequence repeat (SSR) markers from ginger for the first time, providing valuable resources for genetic diversity analysis and molecular verification of ginger genotypes. Gene ontology results indicated that the SSR loci identified in ginger are involved in various biological processes such as metabolism, growth, and development. The validation of 12 novel EST-SSR markers using PCR amplification and capillary electrophoresis revealed a total of 111 alleles, with an average of 9.25 alleles per locus and allele sizes ranging between 115-189bp.
Article
Environmental Sciences
Yunbang Zhang, Jian Gao, Yunhai Zhang, Yuanchao Zou, Xiaojuan Cao
Summary: The elongate loach is an endemic species in the middle and upper reaches of the Yangtze River in China, and is currently endangered due to overfishing and habitat destruction. This study conducted transcriptome sequencing and developed polymorphic SSR markers for the loach, providing a basis for genetic information and further research on population genetics and breeding of this endangered species in China.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Genetics & Heredity
Jin Li, Changbing Zhang, Shiyong Chen, Keke Jiang, Hao Guan, Wenhui Liu
Summary: By analyzing the transcriptome data of E. breviaristatus, this study obtained 30,668 SSR markers and evaluated their transferability and genetic diversity. The results provide a basis for the conservation and management of E. breviaristatus and valuable resources for exploring genetic relationships among species in the Elymus genus.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.