Article
Pharmacology & Pharmacy
Joshua Lott, Emily M. Jutkiewicz, Manojkumar A. Puthenveedu
Summary: The synthetic cannabinoid WIN55,212-2 (WIN) disrupts the Golgi apparatus and microtubule network in cells, independent of cannabinoid receptors. This unique effect may contribute to the physiological and therapeutic effects of WIN.
MOLECULAR PHARMACOLOGY
(2022)
Article
Immunology
Mario Perez-Diego, Alba Angelina, Leticia Martin-Cruz, Andres de la Rocha-Munoz, Angel Maldonado, Carmen Sevilla-Ortega, Oscar Palomares
Summary: This study found that WIN55,212-2 induced the differentiation of hmoDCs that are less responsive to LPS stimulation and able to activate Tregs. Additionally, WIN55,212-2 inhibits the pro-inflammatory polarization of human macrophages by suppressing cytokine production, inflammasome activation, and pyroptotic cell death. These findings shed light on the molecular mechanisms of cannabinoids in exerting anti-inflammatory effects in myeloid cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
M. F. Nagoor Meeran, Farah Laham, Sheikh Azimullah, Charu Sharma, Ahmed Juma Al Kaabi, Saeed Tariq, Ernest Adeghate, Sameer N. Goyal, Shreesh Ojha
Summary: The study found that BCP has significant protective effects in ISO-induced myocardial injury, reducing myocardial damage and regulating multiple signaling pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Senthil Jayarajan, Joseph J. Meissler, Martin W. Adler, Toby K. Eisenstein
Summary: The selective CB2 agonist O-1966 can block the proliferative response of mouse spleen cells and induce regulatory T cells in vitro. In vivo studies showed that O-1966 prolongs skin graft survival time and reduces spleen cell proliferation in response to allogeneic cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Ying Zhang, Amy Gilmour, Young-Hoon Ahn, Laureano de la Vega, Albena T. Dinkova-Kostova
Summary: SFN inhibits mTOR in a concentration- and time-dependent manner through inhibiting HDAC6 and decreasing the catalytic activity of AKT, even in the absence of NRF2.
Article
Pharmacology & Pharmacy
Jia Liu, Zhuojun Liu, Jing Zhang, Xiaofang Chen, Junge Chen, Linlin Sui, Jian Yu
Summary: In this study, the researchers found that Ibrutinib has a stronger cytotoxic effect on endothelial cells compared to Zanubrutinib, while Acalabrutinib has a very weak cytotoxic effect. Ibrutinib was also found to induce endothelial dysfunction through the stimulation of BMP4 expression. The findings suggest the potential use of Ibrutinib in the treatment of angiogenesis-dependent cancers.
Article
Immunology
Kaoru Murakami, Daisuke Kamimura, Rie Hasebe, Mona Uchida, Nobuya Abe, Reiji Yamamoto, Jing-Jing Jiang, Yasuhiro Hidaka, Yuko Nakanishi, Shuzo Fujita, Yuki Toda, Nobuhiro Toda, Hiroki Tanaka, Shizuo Akira, Yuki Tanaka, Masaaki Murakami
Summary: RAP99-LPS, an extract of Rhodobacter azotoformans, acts as a TLR4 agonist to enhance immune system activation, promoting anti-viral and anti-tumor activity in vivo.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Danielle I. Lee, Michael Murninkas, Sigal Elyagon, Yoram Etzion, Hope D. Anderson
Summary: CB13 has no effect on basal hemodynamic properties of rat hearts, but inhibits tachypacing-induced shortening of AERP. CB13 increases protein expression of PGC1 alpha, suggesting upregulation of mitochondrial biogenesis, and diminishes the downregulation of Connexin43 induced by tachypacing. These findings suggest that peripheral activation of CBR could be a new treatment strategy to prevent AR in AF patients.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Ayat Zagzoog, Asher L. Brandt, Tallan Black, Eunhyun D. Kim, Riley Burkart, Mikin Patel, Zhiyun Jin, Maria Nikolaeva, Robert B. Laprairie
Summary: This study assessed select SCRAs recently identified as potential CB1R and CB2R agonists, finding several SCRAs displaying high bias for cAMP inhibition or beta arrestin2 recruitment and receptor subtype selectivity between CB1R and CB2R.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jiangyinzi Shang, Sophie Hines, Meagan J. Makarczyk, Hang Lin, MaCalus V. Hogan, Alan Yan
Summary: Studies on human chondrocytes indicate that the effects of marijuana derivatives on normal cartilage are concentration-dependent, with potential protective effects on cartilage degradation associated with osteoarthritis. However, further research is needed to understand the extent of these effects.
Article
Immunology
Adam Worob, Cody J. Wenthur
Summary: Synthetic cannabinoid receptor agonists (SCRAs) are compounds that mimic the psychoactive components in cannabis and are easily accessible for recreational use. This study developed cross-reactive anti-SCRA bioconjugate vaccines that can detect multiple SCRAs, providing potential diagnostic and therapeutic interventions against SCRA intoxication.
Article
Biochemistry & Molecular Biology
Franziska Kopietz, Yazeed Alshuweishi, Silvia Bijland, Fatmah Alghamdi, Eva Degerman, Kei Sakamoto, Ian P. Salt, Olga Goransson
Summary: The study aimed to evaluate the effects of two AMPK activators on glucose uptake in adipocytes, showing that A-769662 significantly reduced insulin-stimulated glucose uptake, but neither activator had clear effects on Akt/AS160 signaling. The results suggest that AMPK activation alone does not inhibit glucose uptake in adipocytes and that the effects of AICAR and A-769662 are AMPK-independent.
BIOCHEMICAL JOURNAL
(2021)
Article
Pharmacology & Pharmacy
David B. Finlay, Thuy Nguyen, Thomas F. Gamage, Shuli Chen, Daniel G. Barrus, Purvi R. Patel, Brian F. Thomas, Jenny L. Wiley, Yanan Zhang, Michelle Glass
Summary: This study compared the synthetic cannabinoid receptor agonist EG-018 and its analogues with existing putative neutral antagonists of CB1 receptor. The results showed highly variable signaling outcomes for EG-018 analogues, with some compounds demonstrating potential as neutral antagonists. These findings provide important insights for the development of CB1 neutral antagonists.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2022)
Article
Medical Laboratory Technology
Liesl K. Janssens, Dimitri Boeckaerts, Simon Hudson, Daria Morozova, Annelies Cannaert, David M. Wood, Caitlin Wolfe, Bernard De Baets, Michiel Stock, Paul Dargan, Christophe P. Stove
Summary: This study conducted a screening of serum samples using an activity-based method to detect novel synthetic cannabinoid receptor agonists (SCRAs). The results showed that the activity-based screening had excellent performance and the combination with machine learning models enabled automated sample scoring, which could complement conventional analytical methods.
CLINICAL CHEMISTRY
(2022)
Article
Physiology
Stewart Christie, Vladimir Zagorodnyuk
Summary: This study demonstrates the selective inhibitory effect of CB2 receptor agonists on capsaicin-sensitive mucosal bladder afferents, with no impact on muscular-mucosal afferents. This finding may have implications for understanding the role of endocannabinoids in modulating bladder function and sensation in health and diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
