Article
Gastroenterology & Hepatology
Laure Brigitte Kouitcheu Mabeku, Mohamadou Bello Epesse, Stephane Fotsing, Roland Kamgang, Magloire Tchidjo
Summary: In this study, the diagnostic results of rapid urease test, stool antigen test, and serology in detecting H. pylori infection were compared in Cameroon. The stool antigen test showed higher sensitivity and specificity compared to serology, and could be used as a noninvasive method for assessing H. pylori infection. Further diagnostic tests are needed to confirm seropositive cases identified through serological testing.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Medicine, General & Internal
Nesrin Gareayaghi, Bekir Kocazeybek
Summary: Reports suggest an increasing prevalence of clarithromycin resistance in children. This study investigated primary clarithromycin resistance in Helicobacter pylori strains isolated from symptomatic and asymptomatic children. It found a significant difference in point mutations between age groups and symptoms, indicating the potential impact of clarithromycin-resistant H. pylori strains on gastrointestinal symptoms in children.
Article
Gastroenterology & Hepatology
Enming Qiu, Shaoqin Jin, Zhuo Xiao, Qianyun Chen, Qiaohui Wang, Huayong Liu, Chanfang Xie, Chong Chen, Zhou Li, Shuai Han
Summary: The study established a rapid, simple, and inexpensive noninvasive method based on CRISPR-Cas12a technology for detecting Helicobacter pylori in stool specimens. The method demonstrated high sensitivity and specificity, with the ability to distinguish H. pylori from other pathogens, making it a promising supplemental test in clinical practice. Further evaluation against gold standard tests is necessary to assess its sensitivity and specificity.
Article
Chemistry, Analytical
Haimin Zou, Chiliang Lin, Haocheng Zan, Yuyao Hu, Xiaruiyan Xu, Dongsheng Wang, Qiuju Wang, Yaoqi Xie, Chen Zhou
Summary: In this study, a fluorescent aptasensor based on CHA-HCR was developed for rapid and sensitive detection of H. pylori in stool samples. The aptasensor showed a wide detection range and low limit of detection, and the results were highly consistent with the 13C-UBT, highlighting its potential in H. pylori screening and other biomarker analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Infectious Diseases
Magnus Halland, Rashidul Haque, Jost Langhorst, James H. Boone, William A. Petri
Summary: The study evaluated the performance of two novel enzyme immunoassays in detecting H. pylori antigen in stool, showing high sensitivity and specificity for diagnosing H. pylori infection. Comparing to a composite reference method, the H. PYLORI QUIK CHEK (TM) and H. PYLORI CHEK (TM) assays demonstrated excellent clinical performance.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Microbiology
Xiaosen Jiang, Zheng Xu, Tongda Zhang, Yuan Li, Wei Li, Hongdong Tan
Summary: This study utilized whole genome sequencing to analyze the genetic epidemiology of H. pylori, developing the HpTT typing tool. Asia was found to be the largest source of isolates, with Switzerland and Australia as main sources of widespread isolates in their continents. The development of a user-friendly website allows researchers to easily compare and analyze their data against the global database.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Wenwen Liu, Gang Lu, Yu Wang, Zhenghong Chen, Yunyun Gao, Zhipeng Yin, Yi Wu, Xiaoqian Lv, Pengbo Guo, Yinghui Zhao
Summary: In order to eradicate Helicobacter pylori (H. pylori) and reduce the risk of gastric cancer, a novel loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) method was established for H. pylori detection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Manouchehr Ahmadi Hedayati, Delniya Khani, Hamed Bashiri
Summary: This study investigated the correlation between H. pylori virulence genes and Fgf7 gene expression in gastric antral epithelial cells. The results showed that H. pylori infection and pathogenic genes were positively correlated with Fgf7 gene expression, suggesting a potential involvement of H. pylori infection in gastric cancer development through the regulation of Fgf7 expression.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Pharmacology & Pharmacy
Romelia Pop, Alexandru-Flaviu Tabaran, Andrei Paul Ungur, Andrada Negoescu, Cornel Catoi
Summary: Helicobacter pylori is a leading bacterial carcinogen and the main cause of gastroduodenal diseases. The high rates of antimicrobial drug resistance pose challenges to current therapies, making the exploration of new treatment options necessary. Silver nanoparticles offer advantages in improving H. pylori therapy and overcoming drug resistance.
Article
Gastroenterology & Hepatology
Toshihiko Kakiuchi, Muneaki Matsuo, Yasuhisa Sakata, Kazuma Fujimoto
Summary: This study developed a bioluminescent enzyme immunoassay (BLEIA) method to detect Helicobacter pylori antigen in feces. The results showed that the BLEIA method was more sensitive than currently marketed kits, making it a useful tool for diagnosing H. pylori infection, especially in noninvasive testing scenarios like in children.
CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Silvana Bonilla, Jeffrey Goldsmith, Paul Mitchell, Athos Bousvaros
Summary: In this study, a novel next-generation sequencing (NGS) based analysis was used to test the antimicrobial susceptibility of stool samples in pediatric patients. The results were compared with the current gold standard method of gastric biopsy culture. The NGS-based analysis showed high concordance with the traditional method for most antibiotics, except for metronidazole resistance in some patients.
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
(2023)
Review
Gastroenterology & Hepatology
Xinlu Ren, Yanyan Shi, Baojun Suo, Xingyu Yao, Haoping Lu, Cailing Li, Yuxin Zhang, Liya Zhou, Xueli Tian, Zhiqiang Song
Summary: The genotypic testing of clarithromycin resistance from stool specimens is an accurate, convenient, noninvasive, and rapid detection technology, providing a definitive diagnosis of clarithromycin resistance and guiding the rational antibiotic selection.
Article
Gastroenterology & Hepatology
Yusaku Kajihara, Tadashi Shimoyama, Ichiro Mizuki
Summary: This study aimed to investigate the detection performance of stool antigen test (SAT) using different domestically produced kits for Helicobacter pylori (H. pylori) infection during proton pump inhibitor (PPIs) use. The results showed that SAT maintained good sensitivity and specificity, regardless of PPIs use.
Article
Biotechnology & Applied Microbiology
Jia Jia, Chongwen Zhang, Yaqi Liu, Yanqiang Huang, Yuefan Bai, Xudong Hang, Liping Zeng, Dongqing Zhu, Hongkai Bi
Summary: Armeniaspirol A (ARM1) is a potent antibacterial agent against Helicobacter pylori, demonstrating the ability to disrupt bacterial cell membranes, inhibit and eliminate biofilm formation, and show efficient therapeutic efficacy in a mouse model.
MICROBIAL BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
M. Keskin, A. Yavuz
Summary: This study aims to evaluate the diagnostic accuracy of the gastric biopsy Helicobacter pylori antigen stool test and compare it with histopathological evaluation and antigen stool test as reference standards. The results show that the gastric biopsy test has the highest coherence with histopathological evaluation and achieves high sensitivity and specificity in detecting H. pylori. This new method provides a more accurate and efficient way to detect H. pylori and eradicate it without waiting for histopathological evaluation.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Review
Parasitology
Tamara Nordmann, Steffen Borrmann, Michael Ramharter
Summary: Hypersensitivity to artemisinin derivatives is a medically important adverse drug reaction, but its frequency and underlying mechanisms are still unknown. It is suggested to provide alternative drugs for patients who cannot tolerate artemisinin derivatives and continue to develop novel non-artemisinin-based combination therapies.
TRENDS IN PARASITOLOGY
(2022)
Article
Immunology
Sophia Schulte, Janna Heide, Christin Ackermann, Sven Peine, Michael Ramharter, Maria Sophia Mackroth, Robin Woost, Thomas Jacobs, Julian Schulze Zur Wiesch
Summary: This study found that EXP1-specific CD4+ T cells had relatively low ex vivo frequency in malaria patients, with a distinct phenotype compared to bulk CD4+ T cells, showing a highly activated effector memory phenotype with elevated levels of co-inhibitory receptors and activation markers.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Immunology
Johannes Brandi, Cari Lehmann, Lea-Christina Kaminski, Julian Schulze Zur Wiesch, Marylyn Addo, Michael Ramharter, Maria Mackroth, Thomas Jacobs, Mathias Riehn
Summary: The overwhelming activation of T cells in acute malaria is associated with severe outcomes, but T cells expressing co-inhibitory molecules are not exhausted, and acquire additional suppressive capacity, representing a potential immune regulatory pathway to prevent further T cell activation during acute malaria.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Infectious Diseases
Paulin N. Essone, Bayode R. Adegbite, Marien J. M. Mbadinga, Armel Mbouna, Fabrice Lotola-Mougeni, Ayodele Alabi, Jean R. Edoa, Bertrand Lell, Abraham S. Alabi, Ayola A. Adegnika, Michael Ramharter, Joel F. D. Siawaya, Martin P. Grobusch, Peter G. Kremsner, Selidji T. Agnandji
Summary: This study evaluated the diagnostic utility of creatine kinase-MB (CK-MB) and hepcidin (HEPC) for tuberculosis (TB) and found that their levels were statistically different between confirmed TB cases and non-TB cases. Combining them with two other biomarkers can improve the diagnostic accuracy.
Correction
Immunology
Thaisa Lucas Sandri, Andrea Kreidenweiss, Simon Cavallo, David Weber, Sascha Juhas, Miriam Rodi, Tamirat Gebru Woldearegai, Markus Gmeiner, Luzia Veletzky, Michael Ramharter, Gildas B. Tazemda-Kuitsouc, Pierre Blaise Matsiegui, Benjamin Mordmueller, Jana Held
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Quique Bassat, Oumou Maiga-Ascofare, Jurgen May, Jerome Clain, Ghyslain Mombo-Ngoma, Mirjam Groger, Ayola A. Adegnika, Jean-Claude Dejon Agobe, Abdoulaye Djimde, Johannes Mischlinger, Michael Ramharter
Summary: The triple artemisinin-based combination therapy (TACT) is a new treatment paradigm for uncomplicated falciparum malaria, which aims to prevent the development and spread of multidrug resistance more effectively than current artemisinin-based combination therapy (ACT). However, TACT is associated with a slightly higher frequency of adverse drug reactions compared to ACT. The challenge lies in evaluating efficacy and tolerability, and providing objective evidence for the management of patients and prevention of anti-malarial drug resistance by TACT.
Article
Immunology
Mirjam Groger, Peter Akhideno, Christine J. Kleist, Femi O. Babatunde, Osahogie Edeawe, Julia Hinzmann, ThankGod Akhigbe, Joy Nwatuzor, Gloria Eifediyi, Jonas Mueller, Mette Hinrichs, Meike Pahlmann, Francisca Naana Sarpong, Christine Wagner, Anke Thielebein, Louis Aihonwalan, Till Koch, Maria Riedner, Ephraim Ogbaini-Emovon, Sylvanus Okogbenin, Stephan Guenther, Sebastian G. Wicha, Michael Ramharter, Lisa Oestereich, Sophie Duraffour, Cyril Erameh
Summary: This study examines the pharmacokinetics of ribavirin in the treatment of Lassa fever and finds consistent exposure across patients. While ribavirin shows low antiviral efficacy, it may have anti-inflammatory effects. Further research is needed to determine the role of ribavirin in the treatment of Lassa fever.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Melina Heinemann, Lukas Kleinjohann, Thierry Rolling, Doris Winter, Nina Hackbarth, Michael Ramharter, Marylyn Addo, Daniel Eibach, Richard O. Phillips, Alex Owusu-Ofori, Christof D. Vinnemeier
Summary: This longitudinal study aimed to assess the impact of antibiotic intake on the incidence of intestinal extended-spectrum b-lactamase-producing Enterobacterales (ESBL-PE) in Ghanaian pharmacy customers outside of hospitals. The study found that the rate of intestinal ESBL-PE carriage among pharmacy customers increased during the 28 days of follow-up, regardless of antibiotic intake. This highlights the urgent need for improved prevention strategies, development of new antibiotic drugs, and potential future elimination strategies.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Luzia Veletzky, Kirsten Alexandra Eberhardt, Jennifer Hergeth, Daniel Robert Stelzl, Rella Zoleko Manego, Ghyslain Mombo-Ngoma, Ruth Kreuzmair, Gerrit Burger, Ayola Akim Adegnika, Selidji Todagbe Agnandji, Pierre Blaise Matsiegui, Michel Boussinesq, Benjamin Mordmueller, Michael Ramharter
Summary: Loiasis, a filarial disease endemic in Central and West Africa, presents with varying clinical and hematological findings depending on the infection status. Eye worm positivity is associated with a wide range of clinical manifestations, while microfilaremia itself is not; Loa loa infection is correlated with eosinophilia.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Infectious Diseases
Rella Zoleko-Manego, Dearie G. Okwu, Christian Handrich, Lia B. Dimessa-Mbadinga, Malick A. Akinosho, Wilfrid F. Ndzebe-Ndoumba, Saskia D. Davi, Daniel Stelzl, Luzia Veletzky, Andrea Kreidenweiss, Tamara Nordmann, Ayola A. Adegnika, Bertrand Lell, Peter G. Kremsner, Michael Ramharter, Ghyslain Mombo-Ngoma
Summary: The first-line recommended artemisinin-based combination therapies (ACTs) and investigational second-generation ACTs used for the treatment of uncomplicated malaria were found to reduce the excretion of S. haematobium eggs in patients co-infected with urogenital schistosomiasis.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Review
Infectious Diseases
Brice Autier, Bruno Gottstein, Laurence Millon, Michael Ramharter, Beate Gruener, Solange Bresson-Hadni, Sarah Dion, Florence Robert-Gangneux
Summary: This narrative review examines the presentation of alveolar echinococcosis (AE) in immunocompromised patients and provides recommendations for patient management and future research. The findings indicate a significant association between AE and solid organ transplantation, chronic inflammatory/autoimmune diseases, and malignancies, but not HIV infection. AE in immunocompromised patients is characterized by early diagnosis, atypical imaging, and low sensitivity of serology.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Public, Environmental & Occupational Health
Friederike Reinsberg, Mary W. Moehlmann, Ralf Krumkamp, Lena Landsmann, Christian Heitkamp, Johannes Jochum, Marylyn Addo, Michael Ramharter, Christiane Radt, Camilla Rothe, Christof Vinnemeier, Benno Kreuels
Summary: This study investigated whether travelers who used antimalarial chemoprophylaxis experienced symptoms of illness and identified risk factors for non-adherence to prophylaxis. The results showed that the occurrence of symptoms during travel did not differ significantly between participants who used prophylaxis and those who did not. Risk factors for non-adherence to prophylaxis included being under the age of 30, traveling to West or Central Africa, and travel duration exceeding 14 days.
JOURNAL OF TRAVEL MEDICINE
(2023)
Article
Public, Environmental & Occupational Health
Farah Saffar, Melina Heinemann, Christian Heitkamp, Daniel Robert Stelzl, Michael Ramharter, Mirjam Schunk, Camilla Rothe, Silja Buehler
Summary: This study aimed to assess the appropriateness of medical management following suspected rabies exposure (SRE) in international travellers. The results showed that awareness for appropriate first aid and timely seeking of professional treatment is insufficient in German travellers. Travel practitioners need to educate travellers about rabies risk, prevention measures, and the correct behavior after SRE including adequate wound treatment and seeking immediate medical help for prophylaxis.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Cyril O. Erameh, Till Koch, Osahogie Isaac Edeawe, Lisa Oestereich, Till Omansen, Johannes Jochum, Donatus I. Adomeh, Odia Ikponmwonsa, Chris Aire, Meike Pahlmann, Danny A. Asogun, Ephraim Ogbaini-Emovon, Sylvanus A. Okogbenin, Stephan Guenther, Michael Ramharter, Peter E. Akideno, Benno Kreuels
Summary: A study conducted at a hospital in Nigeria utilized POCUS technique to examine LF patients and found various pathological findings such as ascites, pericardial effusion, and pleural effusion. The use of POCUS required minimal resources and training, and it may help guide the clinical management of LF patients.
JOURNAL OF INFECTION
(2023)
Article
Medicine, General & Internal
Dorothea Ekoka Mbassi, Ghyslain Mombo-Ngoma, Jana Held, Dearie Glory Okwu, Wilfrid Ndzebe-Ndoumba, Laura Charlotte Kalkman, Franck Aurelien Ekoka Mbassi, Lais Pessanha de Carvalho, Juliana Inoue, Malik Azeez Akinosho, Lia Betty Dimessa Mbadinga, Emmanuel Koffi Yovo, Benjamin Mordmueller, Peter Gottfried Kremsner, Ayola Akim Adegnika, Michael Ramharter, Rella Zoleko-Manego
Summary: This study assessed the tolerability and efficacy of ivermectin in Gabonese adults with P. falciparum infections. The results showed that ivermectin reduced the asexual parasites similarly to placebo, indicating its potential as a treatment for asymptomatic P. falciparum infections.
