Article
Biochemistry & Molecular Biology
Weiwei Wang, Caiqin Cao, Botao Zhang, Feiyu Wang, Dadong Deng, Jianhua Cao, Hua Li, Mei Yu
Summary: In this study, we analyzed the transcriptomes of the endometrium and myometrium in pigs and found distinct differences between the two layers during early pregnancy. The endometrium was primarily involved in immune response processes, while the myometrium was related to contraction pathways. Additionally, we identified histone modifications associated with gene expression in the two layers, providing insights into the gene regulatory mechanisms during implantation in pigs.
Article
Biotechnology & Applied Microbiology
Natalin Valeff, Damian O. Muzzio, Franziska Matzner, Marcos Dibo, Janine Golchert, Georg Homuth, Martin C. Abba, Marek Zygmunt, Federico Jensen
Summary: Pregnancy alters B cell activation, leading to a state of hypo-responsiveness where B cells have decreased response to BCR stimulation. This may be part of the maternal immune strategy for fetal tolerance.
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak
Summary: The study found that EMF treatment affects gene expression in the myometrium of pigs during the peri-implantation period, mainly involving defense and immune responses. Analysis revealed that DEGs enrich multiple signaling pathways, and also uncovered the regulatory mechanisms of EMF through long non-coding RNAs and single nucleotide variants on the transcriptomic profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
B. Atanasov, T. Dovenski, I Celeska, J. S. Stevenson
Summary: The results suggest that administering 1 dose of PGF(2 alpha) on day 8 instead of day 7 in an Ovsynch program can increase the proportion of cows with complete luteolysis. In the second experiment, cows receiving a second dose of PGF(2 alpha) on day 8 had a significantly higher proportion of complete luteolysis compared to those receiving only one dose on day 7 or day 8. Pregnancy per AI did not differ among the three treatments.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Jaya Bharati, N. H. Mohan, Satish Kumar, Jayashree Gogoi, Sai Kumar, Bosco Jose, Meeti Punetha, Sanjib Borah, Amit Kumar, Mihir Sarkar
Summary: RNA-Seq was used to identify differentially expressed genes (DEGs) in porcine corpus luteum (CL) during different physiological stages, revealing significant enrichment of gene functions in early, mid, and late stages. Apoptosis was most active in the regressed stage. Pathway analysis indicated lipid metabolism, translation, immune, and endocrine system pathways were associated with CL function. Gene network analysis identified novel hub genes AR, FOS, CDKN1A potentially regulating CL physiology.
Article
Reproductive Biology
T. Castro, J. C. Jacob, G. Stefani, R. R. Domingues, O. J. Ginther
Summary: The concentrations of progesterone (P4) and a metabolite of PGF2 alpha (PGFM) in mares show significant variations between the interovulatory interval (IOI) and early pregnancy. The study reveals different patterns of PGFM concentration fluctuations between the IOI and pregnancy, with pregnant mares exhibiting smaller PGFM pulse amplitudes.
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: The pituitary gland plays a crucial role in female reproduction. However, little is known about the changes in the transcriptome of the porcine pituitary during reproductive status. This study aimed to analyze and compare the transcriptome profile of the pituitary gland in pregnant pigs and pigs during the estrous cycle. The analysis revealed 482 differentially expressed genes, with 68 up-regulated and 414 down-regulated in pregnant pigs. These findings provide insights into the molecular mechanisms involved in maternal recognition of pregnancy in pigs.
Article
Endocrinology & Metabolism
Ga-Hyun Son, So Yeon Choi, Yeon-Ji Ju, Keun-Young Lee, Jae Jun Lee, Ji-Eun Song, Youngmi Kim, Sung Taek Park
Summary: The study found significant differences in the whole blood transcriptomic profiles of women with cervical insufficiency compared to controls, suggesting different immune responses in these women may impact pregnancy outcomes.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Miao Zhao, Xian Zou, Shenping Zhou, Jilong Liu, Chong Wang
Summary: In this study, the gene expression profiles of the receptive uterus in guinea pigs were analyzed and compared to the pre-receptive uterus. The results highlighted the importance of the inflammatory response in uterine receptivity and identified several hub genes involved in regulating endometrial receptivity. A cross-species comparison revealed that guinea pigs have a gene expression pattern of uterine receptivity more similar to humans than mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Reproductive Biology
Mariangela Bueno Cordeiro Maldonado, Valeska de Castro Lourenco, Lucas de Oliveira Bezerra, Isabella Rio Feltrin, Adriano Felipe Mendes, Cecilia Constantino Rocha, Guilherme Pugliesi, Alan D. Ealy, Claudia Maria Bertan Membrive, Marcelo Fabio Gouveia Nogueira
Summary: Early embryonic mortality due to maternal-fetal recognition failure is a major issue in the cattle industry. Modifying the levels and ratios of prostaglandin F2a and PGE2 can improve pregnancy establishment in cattle. This study investigated the effects of conjugated linoleic acid (CLA) on PGE2 and PGF2a synthesis and gene expression related to maternal-fetal recognition in bovine trophoblast cells (CT-1). CLA supplementation decreased PGE2 and PGF2a concentrations and influenced the ratio of PGE2 to PGF2a in CT-1. CLA also had a quadratic effect on the expression of MMP9, PTGES2, and PTGER4 genes.
Article
Biochemistry & Molecular Biology
Driele B. Santos, Geysson J. J. Fernandez, Luciana M. C. Pardini, Maria Ines M. C. Pardini, Adriana C. C. Ferrasi
Summary: Studying the transcriptome of canine mammary ductal carcinoma can help identify genes and pathways related to human breast cancer, leading to the discovery of biomarkers and therapeutic targets. This study identified 633 downregulated and 573 upregulated genes in carcinoma tissue compared to normal tissue, indicating deregulation in inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways. The differentially expressed genes may indicate disease aggressiveness and prognosis. The canine transcriptome serves as an excellent model for oncology research in both humans and animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Feng Yang, Hao Wu, Shuaishuai Wu, Daiyong Cui, Jingqian Zhou, Nan Li, Jinliang Ma, Shenming Zeng
Summary: Estrus synchronization in donkeys is important, but research on this topic is limited. This study investigated the effects of cloprostenol on the donkey corpus luteum and designed a successful protocol for estrus synchronization. Different doses and timing of cloprostenol treatment were tested, and the best protocol combining deslorelin, cloprostenol, and JVID was identified. The results showed that treatment with 0.4 mg cloprostenol on the third day following ovulation minimized the length of the luteal phase and estrous cycle. The synchronization rate varied among groups, with the highest rate observed in the group using the combined protocol. Pregnancy rates did not differ among the protocols. In conclusion, cloprostenol effectively induced luteolysis in donkeys, and the combined treatment protocol is efficient for estrus synchronization.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Surgery
J. B. van Praagh, J. G. de Wit, P. Olinga, J. J. de Haan, W. B. Nagengast, R. S. N. Fehrmann, K. Havenga
Summary: Anastomotic leakage in colorectal surgery patients is associated with errors in gene regulation, affecting cell division and various biological processes. The study provides potential gene and biological process targets for the treatment and diagnosis of patients at risk of anastomotic leakage.
BRITISH JOURNAL OF SURGERY
(2021)
Article
Pediatrics
Xiaoyu Pan, Tik Muk, Shuqiang Ren, Duc Ninh Nguyen, Rene L. Shen, Fei Gao, Per Torp Sangild
Summary: This study utilized a preterm pig model to investigate the blood transcriptomics for identifying new early blood biomarkers of NEC progression. The whole-blood transcriptome revealed upregulation of target genes in NEC cases with subtle clinical symptoms, mainly affecting the colon regions. Differential NEC-associated gene expressions could also be detected in dried blood spots, suggesting potential for easy collection of small blood volumes in infants.
PEDIATRIC RESEARCH
(2022)
Article
Oncology
Marta Brunetti, Ioannis Panagopoulos, Valeria Vitelli, Kristin Andersen, Tarjei S. Hveem, Ben Davidson, Ane Gerda Z. Eriksson, Pernille Kristina Bjerre Trent, Sverre Heim, Francesca Micci
Summary: This study aimed to gain insights into the chromosomal and genomic aberration patterns, as well as gene and miRNA expression profiles, in endometrial carcinomas (ECs). The study found that chromosome 1 was frequently rearranged, leading to gain of 1q. PTEN, PDGFRA, PIK3CA, and KIT were the most frequent pathogenic variants, and several other genes and miRNAs involved in carcinogenesis and immune response showed consistent deregulation. The study confirmed the high degree of genetic heterogeneity in EC tumors.
Article
Environmental Sciences
Anita Franczak, Agata Zmijewska, Kamila Zglejc, Mariusz Dziekonski, Ewa Waszkiewicz, Stanislaw Okrasa, Wieslaw Sobotka, Genowefa Kotwica
JOURNAL OF ELEMENTOLOGY
(2016)
Article
Developmental Biology
Hilde Nelis, Bartosz Wojciechowicz, Anita Franczak, Bart Leemans, Katharina D'Herde, Karen Goossens, Pieter Cornillie, Luc Peelman, Ann Van Soom, Katrien Smits
REPRODUCTION FERTILITY AND DEVELOPMENT
(2016)
Article
Developmental Biology
Hilde Nelis, Julie Vanden Bussche, Bartosz Wojciechowicz, Anita Franczak, Lynn Vanhaecke, Bart Leemans, Pieter Cornillie, Luc Peelman, Ann Van Soom, Katrien Smits
REPRODUCTION FERTILITY AND DEVELOPMENT
(2016)
Article
Developmental Biology
Justyna Kolakowska, Serhiy Souchelnytskyi, Ravi Kanth Rao Saini, Anita Franczak
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Developmental Biology
A. Franczak, K. Zglejc, E. Waszkiewicz, B. Wojciechowicz, M. Martyniak, W. Sobotka, S. Okrasa, G. Kotwica
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Developmental Biology
B. Wojciechowicz, G. Kotwica, K. Zglejc, E. Waszkiewicz, A. Franczak
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Agriculture, Dairy & Animal Science
K. Zglejc, M. Martyniak, E. Waszkiewicz, G. Kotwica, A. Franczak
REPRODUCTION IN DOMESTIC ANIMALS
(2018)
Article
Agriculture, Dairy & Animal Science
K. Zglejc, A. Franczak
REPRODUCTION IN DOMESTIC ANIMALS
(2017)
Article
Reproductive Biology
Marcin Martyniak, Anita Franczak, Genowefa Kotwica
REPRODUCTIVE BIOLOGY
(2018)
Article
Agriculture, Dairy & Animal Science
Anita Franczak, Kamila Zglejc-Waszak, Marcin Martyniak, Ewa Monika Waszkiewicz, Genowefa Kotwica
ANIMAL REPRODUCTION SCIENCE
(2018)
Article
Agriculture, Dairy & Animal Science
Marcin Martyniak, Kamila Zglejc-Waszak, Anita Franczak, Genowefa Kotwica
ANIMAL REPRODUCTION SCIENCE
(2018)
Article
Reproductive Biology
K. Zglejc-Waszak, E. M. Waszkiewicz, A. Franczak
Article
Reproductive Biology
Malgorzata Grzesiak, Ewa Waszkiewicz, Magdalena Wojtas, Kinga Kowalik, Anita Franczak
Article
Reproductive Biology
B. Wojciechowicz, J. Kolakowska, K. Zglejc-Waszak, M. Martyniak, G. Kotwica, A. Franczak
Article
Agriculture, Dairy & Animal Science
Agata Zmijewska, Wioleta Czelejewska, Mariusz Dziekonski, Alina Gajewska, Anita Franczak, Stanislaw Okrasa
ANIMAL REPRODUCTION SCIENCE
(2020)