Article
Pharmacology & Pharmacy
Francine Medjiofack Djeujo, Valentina Stablum, Elisa Pangrazzi, Eugenio Ragazzi, Guglielmina Froldi
Summary: This study found that aqueous decoctions of Vernonia amygdalina (VA) leaves and roots, commonly used in traditional African medicine as an antidiabetic remedy, contain luteolin and vernodalol. The research investigated the effects of these compounds on ff-glucosidase activity, glycation, reactive oxygen species formation, and cell viability. The study revealed that luteolin inhibited ff-glucosidase activity and the formation of advanced glycation end products, while vernodalol did not.
Article
Chemistry, Physical
Eslam Hafez, Samy M. Shaban, Min-Hyeong Kim, Ahmed Y. Elbalaawy, Do-gi Pyun, Dong-Hwan Kim
Summary: Wound dressings containing silver nanoparticles have been found to be effective in preventing bacterial infection and promoting wound healing. The preparation of silver nanoparticles with a uniform core-shell structure of poly(tannic acid) showed high antimicrobial activity, although their cytotoxicity hindered practical applications. The addition of natural cell viability enhancers significantly improved cell viability and increased the antimicrobial performance against bacteria. Integrating the prepared nanoparticles into activated carbon fibers for wound-dressing application showed excellent performance compared to commercial products.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Engineering, Chemical
Seema Menon, Jawaher J. Albaqami, Hamida Hamdi, Lincy Lawrence, Jose Padikkala, Shaji E. Mathew, Arunaksharan Narayanankutty
Summary: Oroxylum indicum Vent is a medium-sized deciduous tree that belongs to the family Bignoniaceae. The roots of this tree are used as one of the ten ingredients to prepare the Dasamula formulation in the Ayurvedic system of medicine in India. Although traditional uses are attributed to the medicinal plant, there are limited scientific data on its potential. The present study thus analyzed the cytotoxic and apoptotic potential of the plant against different cancer cells.
Article
Pharmacology & Pharmacy
Mandeep Dahiya, Rajendra Awasthi, Kamal Dua, Harish Dureja
Summary: In this study, magnetic nanoparticles were prepared using an emulsion solvent evaporation method and optimized using Box Behnken design. The optimized nanoparticles showed a mean particle size range of 71.81 +/- 4.4 to 384.1 +/- 10.5 nm, a polydispersity index range of 0.203 +/- 0.008 to 0.777 +/- 0.010, and a zeta potential range of -34.4 +/- 3.1 to -10.2 +/- 0.6 mV. The entrapment efficiency was 84.64 +/- 0.78%. TEM image confirmed the formation of spherical nanoparticles. The optimized formulation released 25.18%-56.63% and 54.38%-90.56% of loaded drug in phosphate buffer and 0.1 N HCl, respectively. The IC50 value of the optimized formulation in HepG2 cell lines was 38.12 μg/mL. The XRD analysis confirmed the amorphous form of the loaded drug. The average surface roughness for the magnetite and optimized formulation was 5.27025 nm and 8.29138 nm, respectively. The magnetization saturation for magnetite and optimized formulation was 28.58 emu/g and 25.46 emu/g, respectively. The electron paramagnetic resonance spectroscopy revealed a g-Lande factor of 4.30 for pure magnetite and 4.24 for the optimized formulation, indicating the superparamagnetic nature of the particles.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Oncology
Zahra Nozhat, Mina S. Khalaji, Mehdi Hedayati, Sima Kheradmand Kia
Summary: The choice of cell viability assays depends on factors such as cost, speed, and complexity. Each method has its advantages and disadvantages, and using at least two methods simultaneously can provide a more comprehensive evaluation of the effects of different agents on different cell types.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mohamed A. Abd Elhamid, Abd Elrahman S. Mandour, Tarek A. Ismail, Ahmed M. Al-Zohairy, Sanaa Almowallad, Leena S. Alqahtani, Ali Osman
Summary: This study analyzed the chemical composition, total phenolic and total flavonoid contents, and evaluated the antioxidant and in vitro cytotoxic activities of eight soybean cultivars. The results revealed that Giza 35 cultivar exhibited the highest total phenolic and total flavonoid contents, as well as the strongest antioxidant and cytotoxic activities.
Article
Biochemistry & Molecular Biology
Sara Ezazi-Toroghi, Shadi Salarinejad, Melika Kamkar-Vatanparast, Parisa Mokaberi, Zeinab Amiri-Tehranizadeh, Mohammad Reza Saberi, Jamshidkhan Chamani
Summary: This study investigated the interaction between calf thymus DNA (ctDNA) and Malathion in the absence and presence of Histone 1 (H1) using various techniques. The results showed that Malathion can quench the fluorescence of ctDNA, and the interaction behavior changes in the presence of H1.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Oncology
Noratiqah Mohtar, Thaigarajan Parumasivam, Amirah Mohd Gazzali, Chu Shan Tan, Mei Lan Tan, Rozana Othman, Siti Sarah Fazalul Rahiman, Habibah A. Wahab
Summary: This review focuses on the currently available nanoparticles for lung cancers and the type of cellular work that can be conducted to evaluate the effectiveness of a nanoparticulate system for this cancer type. Nanoparticles can improve the delivery of anticancer drugs, ensuring stability and bioavailability. Additional research is needed to confirm the safety and efficacy of developed nanoparticles in suitable in vitro and/or in vivo models.
Article
Biochemistry & Molecular Biology
Marketa Havrdova, Iztok Urbancic, Katerina Barton Tomankova, Lukas Malina, Katerina Polakova, Janez Strancar, Athanasios B. Bourlinos
Summary: Fluorescent carbon dots (CDs) are potential tools for cell labeling due to their advantageous properties. In this study, the researchers investigated the intracellular trafficking and cytotoxicity of cationic quaternized carbon dots (QCDs) in human cancer cell lines MCF-7 and HeLa. They found that QCDs uptake in MCF-7 cells resulted in cell death within the first two hours, with faster death at lower doses. QCDs at a concentration of 100 μg/mL entered the nucleus before cellular death, while at 200 μg/mL dose, QCDs penetrated into the nuclear area after causing blebbing of the cellular membrane. In HeLa cells, dose-dependent effects did not occur, but cell death in mitosis and genotoxicity were observed at all doses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Valeriana Pantea, Vitalie Cobzac, Olga Tagadiuc, Victor Palarie, Valentin Gudumac
Summary: Cancer is a global medical problem, with a shortage of specific anticancer drugs and significant side effects. The liver plays a crucial role in drug metabolism and is a major target of anticancer compounds. Thus, it is important to evaluate the cytotoxic effects of potential anticancer substances on liver cells. This study showed that new local thiosemicarbazide coordination compounds (CCT) had a selective effect on hepatocytes in vitro and were less hepatotoxic compared to doxorubicin, indicating their potential in the development of new anticancer drugs.
Article
Chemistry, Applied
Jaggaiah N. Gorantla, Sunaree Choknud, Eko Suyanto, Htun-Htun Win, Yanling Hua, Maniganda Santhi, Sirilak Wangngae, Anyanee Kamkaew, Mariena Ketudat-Cairns, Lalida Rojanathammanee, James R. Ketudat Cairns
Summary: In this study, various hydroxycinnamic and hydroxybenzoic acids were easily converted to phenolic amides (PAMs) through a one-step BOP-mediated amide coupling reaction. Fifteen PAMs were synthesized with yields above 40% and evaluated for cytotoxic activities against four cancer cell lines. Four amides exhibited promising IC50 values ranging from 5 to 55 μM, while tetradecyl-gallic-amide (13) specifically showed significant inhibitory effects on THP-1 leukemia cells with an IC50 value of 3.08 μM. These findings highlight the potential of phenolic amides as anticancer agents.
NATURAL PRODUCT RESEARCH
(2023)
Article
Polymer Science
Ha Pham Le Khanh, Daniel Nemes, Agnes Rusznyak, Zoltan Ujhelyi, Palma Feher, Ferenc Fenyvesi, Judit Varadi, Miklos Vecsernyes, Ildiko Bacskay
Summary: Polyethylene glycols (PEGs) are widely used in cosmetics, consumer care products, and the pharmaceutical industry due to their advantageous properties. However, little research has been done on the comparative study of PEGs with different molecular weights. This study aimed to investigate the effects of PEGs on cells and larvae, and correlate these effects with molecular weight and osmolality. The results showed that the effects of PEGs were correlated with their structure, molecular weight, osmolality, and cytotoxicity.
Article
Chemistry, Physical
Polina Bikmulina, Nastasia Kosheleva, Yuri Efremov, Artem Antoshin, Zahra Heydari, Valentina Kapustina, Valery Royuk, Vasily Mikhaylov, Victor Fomin, Massoud Vosough, Peter Timashev, Yury Rochev, Anastasia Shpichka
Summary: Cell viability is an important parameter for evaluating tissue equivalents and chemicals, and analyzing 3D cell systems requires a combination of multiple methods to obtain accurate results.
Article
Ophthalmology
Wendy Yen Nee See, Fazliana Ismail, Siti Hamimah Sheikh Abdul Kadir, Visvaraja Subrayan
Summary: The study aimed to investigate the effect of irradiated riboflavin on cellular viability in human tenon fibroblasts. The results showed that irradiated riboflavin induced concentration-dependent cell death, with a significant impact up to 48 hours, but the duration of treatment did not significantly affect the outcome. Further investigations are needed to determine the mechanism of cell death, with a postulation of apoptosis in irradiated riboflavin-treated cells.
CURRENT EYE RESEARCH
(2022)
Article
Environmental Sciences
Madhankumar Dhakshinamoorthy, Senthil Kumar Ponnusamy, Udaya Prakash Nyayiru Kannaian, Bhuvaneswari Srinivasan, Sripriya Nannu Shankar, Kannan Kilavan Packiam
Summary: An endophytic wild fungal strain Phyllosticta elongata MH458897 isolated from the Western Ghats region of Sathyamangalam Tiger Reserve Forest, has the potential to produce an effective anticancer drug Camptothecin (CPT). This endophytic fungus produces defense metabolites against plant pathogens, with CPT gaining attention for its effective anticancer activity. Optimized factors such as medium, pH, and incubation time were found to yield the highest amount of CPT, which showed cytotoxic effects on cancer cell lines in vitro.
ENVIRONMENTAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Babita Garg, Sarwar Beg, Ranjot Kaur, Rajendra Kumar, Om Prakash Katare, Bhupinder Singh
JOURNAL OF DRUG TARGETING
(2018)
Article
Immunology
Ravi Kumar Sharma, Shobha Sehgal, Naresh Sachdeva, Rajendra Kumar, Amod Gupta
IMMUNOLOGICAL INVESTIGATIONS
(2019)
Article
Chemistry, Medicinal
Monika Kaurav, Rajendra Kumar, Atul Jain, Ravi Shankar Pandey
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Pharmacology & Pharmacy
Atul Jain, Sumant Saini, Rajendra Kumar, Teenu Sharma, Rajan Swami, Om Prakash Katare, Bhupinder Singh
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Babita Garg, Sarwar Beg, Rajendra Kumar, O. P. Katare, Bhupinder Singh
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Article
Chemistry, Physical
Shivani Uppal, Aashima, Rajendra Kumar, Shweta Sareen, Khushwinder Kaur, S. K. Mehta
APPLIED SURFACE SCIENCE
(2020)
Article
Chemistry, Physical
Shivani Uppal, Pratibha Sharma, Rajendra Kumar, Khushwinder Kaur, Alka Bhatia, S. K. Mehta
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2020)
Article
Toxicology
Avaneesh Kumar Pandey, Rajendra Kumar, Nusrat Shafiq, Ritika Kondel, Shanky Garg, Harish Negi, Sunil Kumar Arora, Neelam Varma, Samir Malhotra
Summary: The study evaluated the clastogenicity potential of nanoformulations of ethionamide and levofloxacin, showing relatively lower chromosomal damage in mice treated with nanoparticles compared to conventional formulations.
HUMAN & EXPERIMENTAL TOXICOLOGY
(2021)
Article
Instruments & Instrumentation
Atul Jain, Teenu Sharma, Rajendra Kumar, O. P. Katare, Bhupinder Singh
Summary: The research focused on the development and evaluation of solid lipid nanoparticles (SLNs) of Raloxifene for enhanced biopharmaceutical performance against breast cancer, showing promising anticancer potential of the developed SLNs.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Oncology
Rajendra Kumar, Janet Mendonca, Olutosin Owoyemi, Kavya Boyapati, Naiju Thomas, Suthicha Kanacharoen, Max Coffey, Deven Topiwala, Carolina Gomes, Busra Ozbek, Tracy Jones, Marc Rosen, Liang Dong, Sadie Wiens, W. Nathaniel Brennen, John T. Isaacs, Angelo M. De Marzo, Mark C. Markowski, Emmanuel S. Antonarakis, David Z. Qian, Kenneth J. Pienta, Drew M. Pardoll, Michael A. Carducci, Samuel R. Denmeade, Sushant K. Kachhap
Summary: The study demonstrates that supraphysiologic levels of testosterone may inhibit a subset of prostate cancer by activating nucleic acid sensors and immune signaling pathways through two autophagy-mediated processes.
Article
Oncology
Channing J. Paller, Lin Wang, Wei Fu, Rajendra Kumar, Jennifer N. Durham, Nilofer S. Azad, Daniel A. Laheru, Ilene Browner, Sushant K. Kachhap, Kavya Boyapati, Thomas Odeny, Deborah K. Armstrong, Christian F. Meyer, Stephanie Gaillard, Julie R. Brahmer, Ivelisse Page, Hao Wang, Luis A. Diaz Jr
Summary: This phase I trial evaluated the safety and recommended dosing of intravenous mistletoe extract in cancer patients. The results showed that mistletoe extract demonstrated manageable toxicities with disease control and improved quality of life. Further research is needed to examine its effect on survival and chemotherapy tolerability.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Laura A. Sena, Rajendra Kumar, David E. Sanin, Elizabeth A. Thompson, D. Marc Rosen, Susan L. Dalrymple, Lizamma Antony, Yuhan Yang, Carolina Gomes-Alexandre, Jessica L. Hicks, Tracy Jones, Kiara A. Bowers, Jillian N. Eskra, Jennifer Meyers, Anuj Gupta, Alyza Skaist, Srinivasan Yegnasubramanian, Jun Luo, W. Nathaniel Brennen, Sushant K. Kachhap, Emmanuel S. Antonarakis, Angelo M. De Marzo, John T. Isaacs, Mark C. Markowski, Samuel R. Denmeade
Summary: This study identifies high androgen receptor (AR) activity as a predictive biomarker for response to bipolar androgen therapy (BAT) and supports a treatment paradigm for prostate cancer involving alternating between AR inhibition and activation.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Oncology
Naiju Thomas, Rajendra Kumar, Deven Topiwala, Kavya Boyapati, Suthicha Kanacharoen, Michael Carducci, Samuel Denmeade, Sushant K. Kachhap
Meeting Abstract
Oncology
Rajendra Kumar, Kavya Boyapati, Naiju Thomas, Deven Topiwala, Suthicha Kanacharoen, Olutosin Owoyemi, Max Coffey, Michael Carducci, Mark C. Markowski, Emmanuel S. Antonarakis, Drew Pardoll, Samuel Denmeade, Sushant K. Kachhap
Article
Pharmacology & Pharmacy
Shradha Bahuguna, Manish Kumar, Gajanand Sharma, Rajendra Kumar, Bhupinder Singh, Kaisar Raza