Article
Biochemistry & Molecular Biology
Siyi Zhang, Fei Yu, Jin Chen, Dan Yan, Danni Gong, Liangbo Chen, Junzhao Chen, Qinke Yao
Summary: A thin film composed of silk peptide, cellulose nanofibrils, and silver nanoparticles was developed for conjunctival reconstruction. The film improved the surface hydrophilicity of the scaffolds and enhanced biocompatibility. In vivo experiments on rabbits demonstrated that the modified film effectively restored the ocular surface structure and function within 12 days.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Jun Song, Zhongda Chen, Luis Larrea Murillo, Dexin Tang, Chen Meng, Xiangli Zhong, Tao Wang, Jiashen Li
Summary: This study demonstrated that the fibrous membranes play a crucial role in preparing tubular scaffolds for muscular artery regeneration. By combining SF with PLLA, the scaffold showed significantly improved cell adhesion rate and proliferation, indicating promising potential for future applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biophysics
Maria I. Echeverria Molina, Kyriakos Komvopoulos
Summary: Polymer degradation and mechanical properties play a crucial role in tissue engineering. In this study, the degradation behavior of electrospun PLLA bilayer microfibrous scaffolds in a pH-neutral medium was investigated. The interplay between chain scission and orderly chain rearrangement during degradation was found to be critical. The bilayer PLLA scaffolds with adequate matrix stiffness showed potential for tissue engineering and drug delivery applications.
JOURNAL OF BIOMECHANICS
(2023)
Article
Chemistry, Physical
Rita Pazmany, Krisztina S. Nagy, Akos Zsembery, Angela Jedlovszky-Hajdu
Summary: In this study, the researchers improved the cell penetration of fibrous hydrogel scaffolds using ultrasonication and enhanced the storing conditions and shelf life through freeze-drying process. The specific load capacity of the scaffold remained stable after ultrasonication, while the elongation break increased.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Pharmacology & Pharmacy
Alexa-Maria Croitoru, Yasin Karacelebi, Elif Saatcioglu, Eray Altan, Songul Ulag, Huseyin Kivanc Aydogan, Ali Sahin, Ludmila Motelica, Ovidiu Oprea, Bianca-Maria Tihauan, Roxana-Cristina Popescu, Diana Savu, Roxana Trusca, Denisa Ficai, Oguzhan Gunduz, Anton Ficai
Summary: This study developed a novel electro-responsive drug delivery system for controlled and localized drug release to target tissues with minimized side effects. The research focused on loading Quercetin (Q) into nanofiber scaffolds for wound dressing applications, demonstrating rapid drug release under electric field stimulation. The fabricated PLA/GO/Q scaffolds showed promising physico-chemical properties, electrical triggering capacity, antimicrobial efficacy, and biocompatibility, offering potential for applications in various therapeutic areas.
Article
Polymer Science
Feiyi Zhang, Lanlan Yu, Zhun Deng, Shuli Liu, Chenxuan Wang, Lei Liu
Summary: The study found that the wetting and anti-bacterial adhesion of a homo poly(amino acid) coating layer were affected by the amino acid composition, but the anti-bacterial adhesion activity was not necessarily related to the wetting property of the amino acid side-chain.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Hao Wang, Hong Xia, Wendan Yang, Zhenzhen Xu, Toshiaki Natsuki, Qing-Qing Ni
Summary: This study demonstrates the use of biomimetic coating technology inspired by mussels to improve the blood compatibility of small-diameter artificial blood vessels. The researchers show that treating the scaffolds with different concentrations of Fe(III) solutions affects the fiber surface morphology and mechanical properties. The results suggest that the TA/Fe(III) coating can enhance the performance of artificial blood vessels.
Review
Biotechnology & Applied Microbiology
Jose Carlos Rodriguez-Cabello, Israel Gonzalez De Torre, Miguel Gonzalez-Perez, Fernando Gonzalez-Perez, Irene Montequi
Summary: The cutting-edge strategies in biomaterials science are focused on mimicking the design of natural systems, particularly the structure of the extracellular matrix (ECM), while incorporating biomechanical and biomolecular cues for biomedical applications. Nanofiber-based scaffolds are actively studied for tissue engineering and regenerative medicine, with a focus on incorporating dynamics, biochemistry and structural features of the native ECM.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juan Uribe-Gomez, Dennis Schoenfeld, Andres Posada-Murcia, Michel-Manuel Roland, Anja Caspari, Alla Synytska, Sahar Salehi, Thorsten Pretsch, Leonid Ionov
Summary: Thermoplastic polyurethane elastomer fabricated via touch spinning technique can mimic the structure of muscle tissues and has important advantages for engineering skeletal muscles.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Weitong Chen, Luke Nichols, Landon Teer, Kailey Clinton, Lauren B. Priddy
Summary: A novel, single-step method of self-polymerized, dopamine-induced nano-hydroxyapatite (nHA) deposition on 3D printed poly(lactic-co-glycolic acid) (PLGA) scaffolds was proposed to enhance the surface properties and cell-material interactions of the scaffolds.
JOURNAL OF MATERIALS SCIENCE
(2022)
Article
Materials Science, Biomaterials
Tien-Li Ma, Shang-Chih Yang, Ting Cheng, Mei-Yun Chen, Jo-Hsuan Wu, Shu-Lang Liao, Wei-Li Chen, Wei-Fang Su
Summary: Poly(gamma-benzyl-l-glutamate) (PBG) biomimetic scaffolds show potential as candidate materials for corneal nerve regeneration and neurotrophic keratopathy treatment. The PBG scaffolds containing aligned fibers guide the growth and extension of trigeminal ganglia cells, and demonstrate low immune response and neurogenesis induction in in vivo experiments.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Biochemistry & Molecular Biology
Matthias Rohmer, Jan Freudenberg, Wolfgang Hubertus Binder
Summary: The secondary structure of poly(amino acids) is a useful tool for studying proteins. This article discusses the secondary structures of homopolymers made from glutamic acid, aspartic acid, and alpha-aminoisobutyric acid, and reviews factors such as side groups, solvent interactions, and chain interactions. The folding of hybrid-polymers, which consist of both oligo(amino acids) and synthetic polymers, is also explored. This overview is part of a larger study on protein fibrillation and supramolecular polymers, and provides insights into amyloid fibrillation, which is crucial for the design of biomolecules.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Multidisciplinary
Katarina McGarry, Eelya Sefat, Taylor C. Suh, Kiran M. Ali, Jessica M. Gluck
Summary: Polymer scaffolds are widely used in tissue engineering to improve the repair and regeneration of damaged tissue. Natural polymers have better cellular adhesion and proliferation, while synthetic polymers are highly tunable. An evaluation of the cellular response to collagen-based and PLA scaffolds showed that NIH3T3 cells preferred collagen-based scaffolds. Incorporating cellular binding mechanisms from natural polymers into biodegradable synthetics offers a promising direction for biomaterials to become biomimetic.
Article
Biochemistry & Molecular Biology
Lokesh Agrawal, Sunil Kumar Vimal, Paolo Barzaghi, Takashi Shiga, Marco Terenzio
Summary: A biomaterial formulation consisting of melanin and PHB has been developed, which exhibits physio-chemical, electrical, and biological compatibility with neural tissues. The blended scaffolds improve cell adhesion and growth of motor and sensory neurons, making it a potential regenerative modality for neural tissue injuries.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Simone I. S. Hendrikse, Rafael Contreras-Montoya, Amanda Ellis, Pall Thordarson, Jonathan W. Steed
Summary: The fundamental building blocks of life, such as nucleotides, amino acids, and saccharides, form various components and hierarchical structures in nature. Mimicking these biological systems and incorporating order and handedness into biomaterial design is necessary, with computational simulations playing a key role in improving biomaterial designs.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Ophthalmology
Lucie Tichotova, Hana Studenovska, Goran Petrovski, Stepan Popelka, Yaroslav Nemesh, Miroslava Sedlackova, Saskia Drutovic, Sonali Rohiwal, Pavla Jendelova, Slaven Erceg, Anna Brymova, Ana Artero-Castro, Lyubomyr Lytvynchuk, Zbynek Stranak, Zdenka Ellederova, Jan Motlik, Taras Ardan
Summary: RPE replacement is an effective modern option for saving vision in cases of retinal degeneration caused by dysfunction of the retinal pigment epithelium. Cultivating RPE cells on PDLLA nanofibrous membranes results in better maturation and long-term survival, making them a plausible source for replacing non-functioning RPEs during cell therapy.
ACTA OPHTHALMOLOGICA
(2022)
Article
Materials Science, Biomaterials
Nisa Irem Buyuk, Didem Aksu, Gamze Torun Kose
Summary: Combining PLA and 3D printer technology holds promise for personalized treatment of bone injuries and defects. A study found that scaffolds with a pore size of 370-460 μm showed the most ideal results in terms of cell retention, proliferation, and differentiation. 3D printed PLA scaffolds with a 60% filling rate were found to be promising for bone tissue engineering applications.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Takahiro Tadokoro, Mariana Bravo-Hernandez, Kirill Agashkov, Yoshiomi Kobayashi, Oleksandr Platoshyn, Michael Navarro, Silvia Marsala, Atsushi Miyanohara, Tetsuya Yoshizumi, Michiko Shigyo, Volodymyr Krotov, Stefan Juhas, Jana Juhasova, Duong Nguyen, Helena Kupcova Skalnikova, Jan Motlik, Hana Studenovska, Vladimir Proks, Rajiv Reddy, Shawn P. Driscoll, Thomas D. Glenn, Taratorn Kemthong, Suchinda Malaivijitnond, Zoltan Tomori, Ivo Vanicky, Manabu Kakinohana, Samuel L. Pfaff, Joseph Ciacci, Pavel Belan, Martin Marsala
Summary: Changing the excitatory neurotransmitter phenotype of secondary spinal cord neurons to inhibitory can successfully treat neuropathic pain without causing detectable side effects.
Article
Biochemistry & Molecular Biology
Lyubomyr Lytvynchuk, Annabelle Ebbert, Hana Studenovska, Richard Nagymihaly, Natasha Josifovska, David Rais, Stepan Popelka, Lucie Tichotova, Yaroslav Nemesh, Jana Cizkova, Jana Juhasova, Stefan Juhas, Pavla Jendelova, Janka Franekova, Igor Kozak, Slaven Erceg, Zbynek Stranak, Brigitte Mueller, Zdenka Ellederova, Jan Motlik, Knut Stieger, Taras Ardan, Goran Petrovski
Summary: The subretinal implantation of hRPE cells on nanofibrous membranes in minipigs was successfully achieved, and the implanted cells showed gradual attenuation over an eight-week observation period. The implantation area exhibited glial reaction and a neuroinflammatory response.
Article
Cell & Tissue Engineering
Jan Strnadel, Hugo M. Dumortier, Dominika Hajduchova, Romana Zahumenska, Vladimir Nosal, Marek Smolar, Juraj Marcinek, Michal Kalman, Sandra Mersakova, Dusan Brany, Stefan Juhas, Jana Juhasova, Hana Studenovska, Barbora Mitruskova, Stanislava Suroviakova, Slavomira Novakova, Henrieta Skovierova, Egon Kurca, Renata Pecova, Lukas Plank, Erika Halasova
Summary: In this study, a new iPS cell line was generated for modeling the sporadic form of ALS. The cell line was derived from skin fibroblasts obtained through reprogramming and characterized through various analyses, demonstrating its normal karyotype and ability to differentiate into cells from three germ layers.
STEM CELL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Lyubomyr Lytvynchuk, Zbynek Stranak, Hana Studenovska, David Rais, Stepan Popelka, Lucie Tichotova, Yaroslav Nemesh, Anastasiia Kolesnikova, Ruslan Nyshchuk, Anna Brymova, Zdenka Ellederova, Jana Cizkova, Jana Juhasova, Stefan Juhas, Pavla Jendelova, Richard Nagymihaly, Igor Kozak, Slaven Erceg, Susanne Binder, Brigitte Muller, Knut Stieger, Jan Motlik, Goran Petrovski, Taras Ardan
Summary: This study presents a surgical technique for subretinal transplantation of cultivated RPE cells using a cell carrier in minipigs. The success rate of this surgical approach was 93.1% in 29 surgeries over the past 8 years. The surgical placement was confirmed using in vivo fundus imaging. These findings provide a reference for future preclinical studies using large-eye animal models.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Biochemistry & Molecular Biology
Santosh Gupta, Lyubomyr Lytvynchuk, Taras Ardan, Hana Studenovska, Georgina Faura, Lars Eide, Ljubo Znaor, Slaven Erceg, Knut Stieger, Jan Motlik, Kapil Bharti, Goran Petrovski
Summary: The retinal pigment epithelium (RPE) plays a crucial role in maintaining the normal physiology of the eye, and damage to the RPE can lead to degenerative eye diseases. Currently, stem cell-derived RPE transplantation is being explored for treating early stages of age-related macular degeneration (AMD). This article focuses on developmental studies and stem cell-based research to understand the biology and derive functional RPEs.
Article
Materials Science, Multidisciplinary
Ilayda Duru, Nisa Irem Buyuk, Gamze Torun Kose, Dylan Widder Marques, Karina Ann Bruce, John Robert Martin, Duygu Ege
Summary: In this study, fullerenol (Ful), a water-soluble derivative of C-60 fullerene with antioxidant activity, is incorporated into calcium phosphate cements (CPCs) to enhance their osteogenic ability. The addition of Ful in CPCs results in improved mechanical performance, antioxidant activity, and cellular osteogenesis. The results suggest that Ful-loaded cements can be a promising additive for bone reconstruction procedures.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Engineering, Biomedical
Kenda Sabouni, Yetkin Ozturk, Erkan Kacar, Gamze Torun Kose, Fatma Nese Kok, Muhammet Kursat Kazmanli, Mustafa Kamil Urgen, Sakip Onder
Summary: In this study, the effect of (Ti, Mg)N thin film coated Ti6Al4V plates and screws on mineralization/osseointegration was investigated. The results showed that the Mg-containing coatings enhanced mineralization, cell attachment, and hydroxyapatite growth, leading to increased attachment at the implant tissue interface.
BIO-MEDICAL MATERIALS AND ENGINEERING
(2023)
Article
Biophysics
Ozge Karabiyik Acar, G. Delal Nozhatzadeh, A. Alperen Tuncer, Gamze Torun Kose, Erhan Aysan
Summary: This study compared the cell transplantation potentials of two commercial alginate brands and found that PRONATAL KF200, with a lower M/G ratio, was more rigid and robust, making it a promising choice for microcapsule preparation.
JOURNAL OF MECHANICS IN MEDICINE AND BIOLOGY
(2023)
Article
Endocrinology & Metabolism
O. Karabiyik Acar, H. Basoglu, M. Kegin, G. D. Nozhatzadeh, E. Hacihasanoglu, A. A. Tuncer, F. Sahin, G. Torun Kose, E. Aysan
Summary: This study aimed to develop a non-surgical transplantation approach for the treatment of chronic hypoparathyroidism. The researchers optimized the encapsulation technique of parathyroid cells to create micro-sized calcium-alginate beads. The findings showed that these microencapsulated cells had high viability and sustained PTH secretion both in vitro and in vivo.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2023)
Article
Polymer Science
Dilruba Baykara, Esra Pilavci, Songul Ulag, Oseweuba Valentine Okoro, Lei Nie, Amin Shavandi, Ayse Ceren Koyuncu, Ozlem Bingol Ozakpinar, Mehmet Eroglu, Oguzhan Gunduz
Summary: The aim of this study was to develop a 3D-printed wound dressing that can release amoxicillin in response to electrical stimulation. This was achieved by incorporating bismuth ferrite nanoparticles into a chitosan-based scaffold. The results showed that the scaffold had a suitable structure for drug loading and release, and the release of amoxicillin was successfully controlled by electrical stimulation.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Gorkem Cemali, Begum Okutan, Erde Can Safak, Gamze Torun Kose
Summary: Poly(propylene fumarate) (PPF) was synthesized and thermally cured with different weight ratios of vinylphosphonic acid (VPA) and diethyl vinylphosphonate (VPES). The biocompatibility and bone formation capacity of the PPF copolymers were evaluated for their potential as bone tissue scaffolds. The results showed that PPF/VPA copolymers had better cell attachment and higher ALP activity compared to PPF/VPES copolymers, indicating their suitability for bone regeneration.
JOURNAL OF POLYMER RESEARCH
(2023)
Article
Environmental Sciences
Ipek Celen Erdem, Ceren Unek, Pinar Akkus Sut, Ozge Karabiyik Acar, Meral Yurtsever, Fikrettin Sahin
Summary: This study aims to investigate the accumulation of polypropylene particles in plants. Through staining and chemical digestion methods, it was found that a significant amount of polypropylene particles accumulated in the roots and stems of maize and wheat plants, with maize showing a higher accumulation. Additionally, the presence of polypropylene particles in the digestion solutions was confirmed.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Medicine, General & Internal
Ozge Karabiyik Acar, Gulcin Delal Nozhatzadeh, Alperen Tuncer, Gamze Torun Kose, Ezgi Hacihasanoglu, Fikrettin Sahin, Erhan Aysan
Summary: This study successfully differentiated thyroid stem/progenitor cells into parathyroid-like cells using Activin A and sonic hedgehog proteins in a co-culture environment. It presents a potentially effective novel method for cell differentiation.
