Journal
FRUITS
Volume 63, Issue 5, Pages 319-323Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1051/fruits:2008030
Keywords
France; Musa sp.; Mycosphaerella fijiensis; Mycosphaerella musicola; disease resistance; methods
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Introduction. This protocol aims at (i) analysing the nature of host-parasite interactions (resistance or susceptibility), and (ii) studying the variability of the pathogenicity of different pathogen populations (intra/inter-specific) with regard to both virulence and aggressiveness using artificial inoculation with Mycosphaerella fijiensis or Mycosphaerella musicola on plants and banana leaf pieces. The principle, key advantages, starting plant material and time required for the method are presented. Materials and methods. Laboratory materials required and details of the sixteen steps of the protocol (conidial inoculum preparation, artificial inoculation on banana plants and artificial inoculation on banana leaf pieces) are described. Possible troubleshooting is discussed. Results. Typical symptoms of the different diseases are obtained after artificial inoculation.
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