Article
Peripheral Vascular Disease
Yuuki Shimizu, Kazuhisa Kondo, Ryo Hayashida, Ken-ichiro Sasaki, Masanori Ohtsuka, Yoshihiro Fukumoto, Shinichiro Takashima, Oto Inoue, Soichiro Usui, Masayuki Takamura, Masashi Sakuma, Teruo Inoue, Tokuichiro Nagata, Yoshihiro J. Akashi, Yoshihiro Yamada, Tamon Kato, Koichiro Kuwahara, Kaoru Tateno, Yoshio Kobayashi, Rei Shibata, Toyoaki Murohara
Summary: This multicenter clinical trial demonstrated that autologous adipose-derived regenerative cell (ADRC) transplantation is safe and effective for therapeutic angiogenesis in patients with critical limb ischemia (CLI). The overall survival rate was 100% at the post-operative period and 6 months follow-up. 94.1% of limbs were free from major amputation for 6 months. The quality of life score, ulcer size, and walking distance also improved in a significant number of patients.
Review
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Marta Rojas-Torres, Ma Carmen Duran-Ruiz
Summary: Critical limb ischemia is the most severe form of peripheral arterial disease, commonly caused by atherosclerosis leading to progressive blockage of arterial vessels. For patients not suitable for surgical intervention, angiogenic cell therapy has emerged as a promising alternative, showing potential for enhancing revascularization by promoting vessel formation and blood flow recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cardiac & Cardiovascular Systems
Weili Shi, Qiqi Xin, Rong Yuan, Yahui Yuan, Weihong Cong, Keji Chen
Summary: Mesenchymal stem cells play a crucial role in angiogenesis and vasculogenesis through their paracrine function, regulating the formation of blood vessels and improving ischemic myocardium. These cells are able to differentiate into various cell lineages and support the survival of myocardial tissue.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Hematology
Liming Zhao, Andrew S. Lee, Koki Sasagawa, Jan Sokol, Yuting Wang, Ryan C. Ransom, Xin Zhao, Chao Ma, Holly M. Steininger, Lauren S. Koepke, Mimi R. Borrelli, Rachel E. Brewer, Lorene L. Y. Lee, Xianxi Huang, Thomas H. Ambrosi, Rahul Sinha, Malachia Y. Hoover, Jun Seita, Irving L. Weissman, Joseph C. Wu, Derrick C. Wan, Jun Xiao, Michael T. Longaker, Patricia K. Nguyen, Charles K. F. Chan
Summary: The study identified two populations of vascular stem/progenitor cells (VSPCs) from adipose-derived mesenchymal stromal cells. Cotransplantation of VSPC1 and VSPC2 could form functional vessels and improve perfusion in the mouse hindlimb ischemia model.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Wahiba Dhahri, Sylvie Dussault, Nozha Raguema, Michel Desjarlais, Alain Rivard
Summary: This study found that Riociguat, an sGC stimulator, promotes angiogenesis and improves neovascularization after ischemia. The mechanism involves the activation of the p44/p42 MAP kinase pathway and an increase in the number and functions of PACs. This discovery could potentially serve as a new therapeutic strategy for reducing tissue ischemia in patients with severe atherosclerotic diseases.
Article
Peripheral Vascular Disease
Seyedeh-Leili Asadi-Yousefabad, Pegah Nammian, Seyed Mohammad Bagher Tabei, Sajad Daneshi, Jafar Fallahi, Vahid Razban, Mohammad Hasan Sheikhha
Summary: This study aimed to enhance the therapeutic potential of AT-MSCs by overexpressing miR-126 and provide an effective treatment for angiogenesis in CLI. The results demonstrated that the miR-126 treated group showed better outcomes in function scores, VEGF-A levels, and histopathology compared to the control group. Furthermore, miR-126 downregulated the expression of PIK3R2 and SPRED1 and prolonged the survival of MSCs in the muscles.
MICROVASCULAR RESEARCH
(2022)
Article
Cell & Tissue Engineering
Xing Zhang, Yihong Jiang, Qun Huang, Zhaoyu Wu, Hongji Pu, Zhijue Xu, Bo Li, Xinwu Lu, Xinrui Yang, Jinbao Qin, Zhiyou Peng
Summary: Exosomes secreted by ADSCs overexpressing GLO-1 promoted endothelial cell protection and angiogenesis in diabetic mice with limb ischemia, showing promising potential for clinical treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Christy Wing Tung Wong, Apurva Sawhney, Yalan Wu, Yi Wah Mak, Xiao Yu Tian, Hon Fai Chan, Anna Blocki
Summary: This study demonstrated that functional BDACs can be sourced under xeno- and/or serum-free conditions and showed superior therapeutic effects compared to traditional MSCs in a murine model of CLI. The secretome of BDACs contained a larger number of proteins with pro-angiogenic and anti-inflammatory properties, potentially contributing to their enhanced therapeutic potential.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Junya Suzuki, Yuuki Shimizu, Kazuhito Tsuzuki, Zhongyue Pu, Shingo Narita, Shukuro Yamaguchi, Takeshi Katagiri, Etsuo Iwata, Tomohiro Masutomi, Yusuke Fujikawa, Rei Shibata, Toyoaki Murohara
Summary: In this study, we demonstrated that local injection of ADRCs can promote angiogenesis in response to tissue ischemia without promoting remote tumor growth in a mouse model. Our findings indicate that therapeutic angiogenesis to the ischemic hindlimb using ADRCs seems to be safe regarding remote tumor growth.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hyeok Kim, Soon-Jung Park, Jae-Hyun Park, Sunghun Lee, Bong-Woo Park, Soon Min Lee, Ji-Won Hwang, Jin-Ju Kim, Byeongmin Kang, Woo-Sup Sim, Hyo-Jin Kim, Seung Hwan Jeon, Dong-Bin Kim, Jinah Jang, Dong-Woo Cho, Sung-Hwan Moon, Hun-Jun Park, Kiwon Ban
Summary: Researchers have successfully used a combination of two different types of stem cells to repair failed hearts by creating new blood vessels. This dual stem cell treatment improves cardiac function and results in a higher number of larger and stronger new blood vessels.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Engineering, Biomedical
Gyu Tae Park, Jae Kyung Lim, Eun-Bae Choi, Mi-Ju Lim, Bo-Young Yun, Dae Kyoung Kim, Jung Won Yoon, Yoon Gi Hong, Jae Hoon Chang, Seong Hwan Bae, Jung Yong Ahn, Jae Ho Kim
Summary: In this study, microvascular fragments, stromal vascular fractions, and mesenchymal stem cells were isolated from human lipoaspirate and their therapeutic efficacy and in vivo vasculogenic activity were investigated. The results showed that microvascular fragments had improved engraftment efficiency and vasculogenic activity compared to mesenchymal stem cells and stromal vascular fractions, making them highly useful for treating ischemic diseases and in tissue engineering.
BIOMATERIALS RESEARCH
(2023)
